CN213246595U

CN213246595U - Biological freezing instrument

Info

Publication number: CN213246595U
Application number: CN202021897531.2U
Authority: CN
Inventors: 奉伟平; 谭启忠; 谢正; 刘必贵; 卜发平; 徐烈
Original assignee: Chongqing Benaji Liquid Nitrogen Biological Container Co ltd
Current assignee: Chongqing Benaji Liquid Nitrogen Biological Container Co ltd
Priority date: 2020-09-02
Filing date: 2020-09-02
Publication date: 2021-05-25
Anticipated expiration: 2030-09-02

Abstract

The utility model discloses a biological freezing instrument, which comprises a freezing cabinet with an opening at one side and a cabinet door covering the opening of the freezing cabinet, wherein a fan and a liquid nitrogen pipe surrounding the fan are arranged in the freezing cabinet, the rotating shaft of the fan is connected with a motor, the surface of the liquid nitrogen pipe facing the fan is provided with a liquid nitrogen spray hole, the inlet of the liquid nitrogen pipe is provided with a solenoid valve, a temperature sensor is also arranged in the freezing cabinet, the motor, the solenoid valve and the temperature sensor are electrically connected with a central control device, setting a proper cooling rate on a central control device according to the freezing characteristics of the organism, putting the biological product to be frozen into a freezer, the electromagnetic valve is opened to inject liquid nitrogen into the liquid nitrogen pipe, the liquid nitrogen is sprayed out from the liquid nitrogen spray holes, the fan blows the liquid nitrogen to the biological product to freeze the biological product, and the temperature sensor monitors the temperature in the freezer cabinet in real time, so that the problem that the freezing speed is too fast or too slow can be effectively solved. Practice proves that after temperature compensation, the freezing rate of 1-3 ℃ per minute can greatly improve the survival rate of the frozen cells.

Description

Biological freezing instrument

Technical Field

The utility model relates to a cryo-preservation apparatus technical field especially relates to a biological freezing appearance.

Background

Successful cryopreservation of biological samples depends on the rate of freezing, precise temperature control and reasonable temperature gradient (+ 50 ℃ -180 ℃). When the temperature is reduced to-180 ℃, most biological samples can be transferred to liquid nitrogen for long-term storage. During the process of cell cryopreservation, liquid water is converted into ice and cell metabolism is stopped. Meanwhile, water is lost, so that the concentration of salt and metabolites in the cells is changed, the osmotic pressure imbalance is caused, and the recovery of the frozen cells is directly influenced. Meanwhile, the freezing rate is too fast or too slow, which easily causes the loss of water in cells or the formation of large ice crystals, and is not favorable for the survival of the cells.

Those skilled in the art have therefore endeavored to develop a bio-freezer that allows for better control of the cooling temperature and rate.

Disclosure of Invention

In view of the above-mentioned defects in the prior art, the technical problem to be solved by the present invention is to provide a method.

In order to realize the purpose, the utility model provides a biological freezing appearance, close including one side open-ended freezer chest and lid cabinet door on the freezer chest opening, install the fan in the freezer chest and center on the liquid nitrogen pipe of fan, the pivot of fan is connected with the motor, the liquid nitrogen pipe towards the surface of fan is provided with the liquid nitrogen orifice, the entry of liquid nitrogen pipe is provided with the solenoid valve, still install temperature sensor in the freezer chest, motor, solenoid valve and temperature sensor electricity are connected with central control unit.

The cabinet door comprises a door panel and a first partition board which are stacked and assembled, and a continuous first sealing ring is pasted on one side, facing the opening of the freezer, of the edge of the first partition board.

A first heat insulation cavity is arranged between the door panel and the first partition plate, and first heat insulation foam is filled in the first heat insulation cavity.

The middle part of the first partition plate is provided with a hole, and the hole is covered with a second partition plate.

The central control device is electrically connected with a touch display screen arranged on the surface of the freezer.

The freezer includes that one side open-ended shell and unsettled install supporter in the shell, the supporter is just right the both sides wall of fan is provided with the ventilation net respectively, a lateral wall of shell is provided with just right the ventilation net and with the gas vent of mesh intercommunication on the ventilation net.

The outer wall of supporter with be provided with the thermal-insulated chamber of second between the shell, the thermal-insulated intracavity of second is filled there is the thermal-insulated foam of second.

The shell faces to the surface of the cabinet door is further provided with a sealing panel, and the sealing panel is attached with a second sealing ring.

The freezer cabinet is characterized by further comprising mounting brackets mounted at four corners in the shell, and the storage rack is mounted and fixed on the mounting brackets.

One of the mounting brackets is integrally connected with a temperature sensor connecting frame.

The utility model has the advantages that: the biological freezing instrument of the utility model comprises a freezing cabinet with an opening at one side and a cabinet door covered on the opening of the freezing cabinet, a fan and a liquid nitrogen pipe surrounding the fan are arranged in the freezing cabinet, the rotating shaft of the fan is connected with a motor, the surface of the liquid nitrogen pipe facing the fan is provided with liquid nitrogen spray holes, the inlet of the liquid nitrogen pipe is provided with a solenoid valve, a temperature sensor is also arranged in the freezing cabinet, the motor, the solenoid valve and the temperature sensor are electrically connected with a central control device, setting a proper cooling rate on a central control device according to the freezing characteristics of the organism, putting the biological product to be frozen into a freezer, the electromagnetic valve is opened to inject liquid nitrogen into the liquid nitrogen pipe, the liquid nitrogen is sprayed out from the liquid nitrogen spray holes, the fan blows the liquid nitrogen to the biological product to freeze the biological product, and the temperature sensor monitors the temperature in the freezer cabinet in real time, so that the problem that the freezing speed is too fast or too slow can be effectively solved. Practice proves that after temperature compensation, the freezing rate of 1-3 ℃ per minute can greatly improve the survival rate of the frozen cells.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

fig. 2 is a schematic view of the back structure of the cabinet door of the present invention;

fig. 3 is a side view of the cabinet door of the present invention;

FIG. 4 is a cross-sectional view of the structure at A-A in FIG. 3;

FIG. 5 is an exploded view of the present invention;

fig. 6 is a schematic view of a partial structure of the present invention.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and examples, wherein it is noted that, in the description of the invention, the terms "upper", "lower", "left", "right", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular manner, and therefore should not be construed as limiting the present invention. The terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

As shown in fig. 1 to 6, a biological freezing instrument comprises a freezing cabinet 1 with an opening on one side and a cabinet door 2 covering the opening of the freezing cabinet 1, a fan 3 and a liquid nitrogen pipe 4 surrounding the fan 3 are installed in the freezing cabinet 1, a motor 5 is connected with a rotating shaft of the fan 3, a liquid nitrogen spray hole is arranged on the surface of the liquid nitrogen pipe 4 facing the fan 3, an electromagnetic valve is arranged at the inlet of the liquid nitrogen pipe 4, a temperature sensor is also installed in the freezing cabinet 1, the motor 5, the electromagnetic valve and the temperature sensor are electrically connected with a central control device, setting a proper cooling rate on a central control device according to the freezing characteristics of the organism, putting the biological product to be frozen into a freezer, the electromagnetic valve is opened to inject liquid nitrogen into the liquid nitrogen pipe, the liquid nitrogen is sprayed out from the liquid nitrogen spray holes, the fan blows the liquid nitrogen to the biological product to freeze the biological product, and the temperature sensor monitors the temperature in the freezer cabinet in real time, so that the problem that the freezing speed is too fast or too slow can be effectively solved. Practice proves that after temperature compensation, the freezing rate of 1-3 ℃ per minute can greatly improve the survival rate of the frozen cells.

In this embodiment, still install fan 3 in the freezer 1 and around fan 3 and the electric heating pipe 20 of being connected with central control device electricity, electric heating pipe 20 can provide heating function, it is more accurate to control the temperature like this, central control device electricity is connected with the touch display screen 22 of installing on freezer 1 surface, convenient to use's human-computer interface, can be convenient write and input the lift temperature program on touch display screen, and look over the incasement temperature that temperature sensor measures in the freezer 1 in real time, operating condition, and the suggestion information of making mistakes.

The cabinet door 2 comprises a door panel 6 and a first clapboard 7 which are assembled in a stacking way, a continuous first sealing ring 10 is pasted on one side of the edge of the first clapboard 7 facing the opening of the freezer 1, a handle 21 is installed on the door panel 6, a first heat insulation cavity 8 is arranged between the door panel 6 and the first clapboard 7, and first heat insulation foam is filled in the first heat insulation cavity 8; the middle part of the first clapboard 7 is provided with a hole, the hole is covered with a second clapboard 9, and the heat insulation effect is good.

Freezer 1 includes one side open-ended shell 11 and unsettled supporter 12 of installing in shell 11, and freezer 1 is still including installing the installing support 16 in four angles departments in shell 11, and one of them installing support 16 integration is connected with temperature sensor link 17, and temperature sensor link 17 is used for fixed temperature sensor.

The shelf 12 is fixedly arranged on the mounting bracket 16, a second heat insulation cavity 13 is arranged between the outer wall of the shelf 12 and the shell 11, and second heat insulation foam is filled in the second heat insulation cavity 13; the surface of the casing 11 facing the cabinet door 2 is further provided with a sealing panel 14, and the sealing panel 14 is attached with a second sealing ring 15. The diameter of first sealing washer 10 is greater than the diameter of second sealing washer 15, and like this at cabinet door 2 lid back of closing, first sealing washer 10 and second sealing washer 15 play double-deck sealed effect, play the heat preservation effect.

The shelf 12 is provided with ventilating nets 18 on both sides facing the fan 3, one side wall of the shell 11 is provided with an exhaust port 19 facing the ventilating net 18 and communicated with the net hole on the ventilating net 18, the exhaust port 19 is provided with a second fan electrically connected with the central control device, and the exhaust port 19 is used for exhausting redundant cold air in the freezer cabinet 1, so that balance of internal and external pressure is ensured, air convection is increased, and uniformity of temperature is improved.

The utility model discloses a stainless steel structure, silence, first adiabatic foam and the adiabatic foam of second are the whole foaming of polycarboxylate and form, and intensity is high, can effectively reduce the box and warp. The unique annular liquid nitrogen injection design has uniform and consistent freezing effect, and prevents the frozen and stored sample from being prematurely crystallized.

In addition, the two electromagnetic valves are connected in parallel, the two electromagnetic valves are controlled, the two electromagnetic valves are used alternately, and the service life of the electromagnetic valves is greatly prolonged.

The central control device can also be externally connected with a printer and used for printing a temperature control report.

The utility model discloses both can be used for IVF, also can be used for the freezing of cardiac muscle cell, lymphocyte, tissue bank, bone marrow cell, tumor cell, plant cell.

The utility model discloses a theory of operation is:

the method is characterized in that a proper temperature rising and falling rate is set on a central control device according to the freezing characteristics of organisms, biological products to be frozen are placed in a freezing cabinet, a solenoid valve is opened to inject liquid nitrogen into a liquid nitrogen pipe, the liquid nitrogen is sprayed out from a liquid nitrogen spray hole, a fan blows the liquid nitrogen to the biological products through a ventilation net 18 on one side to freeze the biological products, redundant cold air is discharged through an exhaust port 19 through the ventilation net 18 on the other side, balance of internal and external pressure is guaranteed, accurate temperature rise can be carried out through an electric heating pipe 20 in the freezing process, the temperature sensor is guaranteed to monitor the temperature in the freezing cabinet in real time, and the problem that the freezing rate is too fast or too slow. Practice proves that after temperature compensation, the freezing rate of 1-3 ℃ per minute can greatly improve the survival rate of the frozen cells.

The foregoing has described in detail preferred embodiments of the present invention. It should be understood that numerous modifications and variations can be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions that can be obtained by a person skilled in the art through logic analysis, reasoning or limited experiments based on the prior art according to the concepts of the present invention should be within the scope of protection defined by the claims.

Claims

1. A biological freezing instrument is characterized in that: close including one side open-ended freezer chest (1) and lid cabinet door (2) on freezer chest (1) opening, install fan (3) in freezer chest (1) and center on the liquid nitrogen pipe (4) of fan (3), the pivot of fan (3) is connected with motor (5), liquid nitrogen pipe (4) towards the surface of fan (3) is provided with the liquid nitrogen orifice, the entry of liquid nitrogen pipe (4) is provided with the solenoid valve, still install temperature sensor in freezer chest (1), motor (5), solenoid valve and temperature sensor electricity are connected with central control device.

2. The biological freezer of claim 1, wherein: the cabinet door (2) comprises a door panel (6) and a first partition plate (7) which are stacked and assembled, and a continuous first sealing ring (10) is pasted on one side of the edge of the first partition plate (7) facing the opening of the freezer cabinet (1).

3. The biological freezer of claim 2, wherein: a first heat insulation cavity (8) is arranged between the door panel (6) and the first partition plate (7), and first heat insulation foam is filled in the first heat insulation cavity (8).

4. The biological freezer of claim 2, wherein: the middle part of the first clapboard (7) is provided with an opening, and the opening is covered with a second clapboard (9).

5. The biological freezer of claim 1, wherein: the central control device is electrically connected with a touch display screen (22) arranged on the surface of the freezer (1).

6. The biological freezer of claim 1, wherein: freezer (1) includes one side open-ended shell (11) and unsettled installation supporter (12) in shell (11), supporter (12) are just right the both sides wall of fan (3) is provided with ventilation net (18) respectively, a lateral wall of shell (11) is provided with just right ventilation net (18) and with exhaust port (19) of ventilation net (18) net surfing hole intercommunication.

7. The biological freezer of claim 6, wherein: a second heat insulation cavity (13) is arranged between the outer wall of the commodity shelf (12) and the shell (11), and second heat insulation foam is filled in the second heat insulation cavity (13).

8. The biological freezer of claim 6, wherein: the shell (11) faces to the surface of the cabinet door (2) and is further provided with a sealing panel (14), and the sealing panel (14) is attached with a second sealing ring (15).

9. The biological freezer of claim 6, wherein: freezer (1) is still including installing support (16) of four angles departments in shell (11), supporter (12) installation is fixed on installing support (16).

10. The biological freezer of claim 9, wherein: one of the mounting brackets (16) is integrally connected with a temperature sensor connecting frame (17).