CN213240145U - Portable soil enzyme activity detection device - Google Patents
Portable soil enzyme activity detection device Download PDFInfo
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- CN213240145U CN213240145U CN202021913158.5U CN202021913158U CN213240145U CN 213240145 U CN213240145 U CN 213240145U CN 202021913158 U CN202021913158 U CN 202021913158U CN 213240145 U CN213240145 U CN 213240145U
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Abstract
The utility model discloses a portable soil enzyme activity detection device, which comprises a dissolving bottle and a cover body; the dissolving bottle is a transparent container with an opening at the top; a sieve plate is fixed in the middle of the dissolving bottle; the sieve plate divides the interior of the dissolving bottle into a solid chamber and a liquid chamber; the outer side wall of the dissolving bottle is respectively provided with a scale mark corresponding to the solid chamber and the liquid chamber; the side wall of the dissolving bottle is provided with a liquid inlet corresponding to the liquid cavity, and the liquid inlet is positioned above the liquid cavity and below the sieve plate; the bottom of the dissolving bottle is provided with a liquid outlet; the cover body is detachably connected with the top opening of the dissolving bottle; the top of the cover body is provided with a feed inlet. The utility model discloses perfect soil solution extraction structure on current portable soil enzyme activity detection device's basis, add solid cavity and liquid cavity with soil and buffer respectively according to the scale mark, then will dissolve the bottle and invert the mixture, will dissolve the bottle again and just put the separation, easy operation is convenient, has improved the convenience that the portable field of soil enzyme activity detected.
Description
Technical Field
The utility model relates to a soil detection technology field, more specifically the utility model relates to a portable soil enzyme activity detection device that says so.
Background
In the technical field of soil detection, carbonic anhydrase is a zinc-containing metalloenzyme capable of catalyzing CO2With HCO3 -The conversion reaction between them. The carbonic anhydrase can accelerate the dissolution of carbonate rock and promote the deposition of carbonate, so that the carbonic anhydrase can have close relation with the carbon cycle of the global karst ecosystem, and the deep research on the carbon cycle of the karst ecosystem has important significance for revealing the carbon cycle rule in nature and reducing global carbon emission. The karst ecosystem is an important component of the land ecosystem, and the global karst area is 2200 km2The area of land is 15 percent, while the area of karst in China is 1/3 percent of the area of soil in China, which is a country where the karst is very developed, in order to scientifically know the function of carbonic anhydrase in the carbon cycle of a karst ecosystem, the distribution and activity of the carbonic anhydrase in the natural ecological environment of the karst, including soil and water, need to be known.
The current method for measuring carbonic anhydrase activity generally adopts a colorimetric method and a pH meter method (Brinkman,1933), wherein the pH meter method and the improved method thereof are most widely applied. The method requires the use of a pH meter, stopwatch, CO2Steel cylinders, and a reaction vessel and related reagents providing a low temperature reaction environment of less than 10 ℃. The method is usually carried out indoors by using a pH meter and a stopwatch to record the catalytic saturation CO of a sample2The time required for the carbonation of the water to cause a certain change in the pH value, and thus the activity of the carbonic anhydrase was calculated.
In the prior patent, the granted publication number is CN204958937U, the name is a portable soil/water sample carbonic anhydrase activity detection device, which provides a portable soil enzyme activity detection device for field measurement, but before measurement, quantitative extraction of soil dissolving liquid is needed, and the extraction is not beneficial to precise calculation in the field, which brings trouble to the use of the portable soil enzyme activity detection device in the field.
Therefore, how to provide a portable device for detecting the activity of the soil enzyme, which is convenient for the soil dissolving liquid to be accurately extracted, is a problem that needs to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a portable soil enzyme activity detection device aims at solving above-mentioned technical problem.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a portable soil enzyme activity detection device comprising: dissolving the bottle and the cap body;
the dissolving bottle is a transparent container with an opening at the top; a sieve plate is radially fixed in the middle of the dissolving bottle; the sieve plate divides the interior of the dissolving bottle into an upper solid chamber and a lower liquid chamber; the outer side wall of the dissolving bottle is respectively provided with a scale mark corresponding to the solid chamber and the liquid chamber; the side wall of the dissolving bottle is provided with a liquid inlet corresponding to the liquid cavity, and the liquid inlet is positioned above the liquid cavity and below the sieve plate; the bottom of the dissolving bottle is provided with a liquid outlet;
the cover body is detachably connected with the top opening of the dissolving bottle; the top of the cover body is provided with a feed inlet.
Through the technical scheme, the utility model discloses perfect soil solution extraction structure on current portable soil enzyme activity detection device's basis, add soil and buffer solution into solid cavity and liquid cavity according to demand volume reference scale respectively, then invert the dissolution bottle, make the buffer solution pass through the sieve by the liquid cavity and get into the solid cavity and mix, after mixing one end time, put the dissolution bottle upright again, make the soil solution after the mixture separate gradually and leak into the liquid cavity, can extract or pour the soil solution of corresponding demand through the liquid outlet; the operation is simple and convenient, and the convenience of portable field detection of the soil enzyme activity is further improved.
Preferably, in the portable soil enzyme activity detection device, the top edge of the sieve plate is provided with a plurality of elastic clamping strips; and filter paper is laid on the top surface of the sieve plate, and the elastic clamping strips compress the edges of the filter paper. Through laying filter paper, can further prevent that soil from getting into in the liquid cavity, improve the cleanliness factor of soil solution, the follow-up experiment of being convenient for.
Preferably, in the above portable soil enzyme activity detection device, the liquid inlet, the liquid outlet and the feed inlet are provided with sealing plugs. The problem of liquid leakage generated when the placing direction is switched is prevented.
Preferably, in the portable soil enzyme activity detection device, the dissolution bottle and the cover body are connected by screw fastening. Convenient to detach easily clears up inside.
Preferably, in the portable soil enzyme activity detection device, a lower bracket is fixed at the bottom of the dissolution bottle; the lower support comprises a plurality of lower support rods and lower seat rings, wherein the lower support rods are vertically fixed with the bottom surface of the dissolving bottle, and the lower seat rings are fixed at the ends of the lower support rods. The stability of the dissolving bottle is stronger when the dissolving bottle is placed, the dissolving bottle does not need to be held by hands, and the dissolving bottle can be directly placed on a plane.
Preferably, in the above portable soil enzyme activity detection device, the length of the liquid outlet from the bottom surface of the dissolution bottle is smaller than the length of the lower ring from the bottom surface of the dissolution bottle. The lower seat ring and the liquid outlet are prevented from position interference.
Preferably, in the portable device for detecting an activity of an agrohorticultural enzyme, an upper bracket is fixed to a top surface of the cover; the upper bracket comprises a plurality of upper supporting rods vertically fixed on the top surface of the cover body and an upper seat ring fixed at the end of the upper supporting rods. The stability of the dissolving bottle is stronger when the dissolving bottle is inverted, the dissolving bottle does not need to be held by hands and can be directly placed on a plane.
Preferably, in the above portable device for detecting an activity of an enzyme in soil, a length of the feed inlet from the top surface of the cover body is smaller than a length of the upper seat ring from the top surface of the cover body. The upper seat ring is prevented from generating position interference with the feed inlet.
Preferably, in the portable soil enzyme activity detection device, the maximum scale of the scale mark corresponding to the solid chamber is more than 30 ml. The splendid attire demand of experiment can be satisfied.
Preferably, in the portable soil enzyme activity detection device, the maximum scale of the scale corresponding to the liquid chamber is greater than 30 ml. The splendid attire demand of experiment can be satisfied.
According to the technical scheme, compare with prior art, the utility model discloses a portable soil enzyme activity detection device is provided, perfect soil solution extraction structure on current portable soil enzyme activity detection device's basis, add soil and buffer into solid cavity and liquid cavity according to demand volume reference scale mark respectively, then invert the dissolving bottle, make the buffer pass through the sieve by the liquid cavity and get into the solid cavity and mix, after mixing one end time, just put the dissolving bottle again, make the soil solution after the mixture separate gradually and leak into the liquid cavity, can extract or pour the soil solution of corresponding demand through the liquid outlet; the operation is simple and convenient, and the convenience of portable field detection of the soil enzyme activity is further improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic structural view of the external form provided by the present invention;
FIG. 2 is a structural sectional view of the present invention;
FIG. 3 is a sectional view of the structure of the present invention for adding soil and buffer solution into the solid chamber and the liquid chamber respectively;
FIG. 4 is a sectional view of the mixing structure of soil and buffer solution provided by the present invention;
fig. 5 is a structural sectional view of the soil dissolving liquid obtained by standing according to the present invention.
Wherein:
1-dissolving the bottle;
11-sieve plate; 111-elastic snap strips; 12-a solid chamber; 13-a liquid chamber; 14-scale mark; 15-liquid inlet; 16-a liquid outlet;
2-a cover body;
21-a feed inlet;
3-filter paper;
4-lower support;
41-lower support rod; 42-a lower seat ring;
5-mounting a bracket;
51-upper struts; 52-upper seat ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 and fig. 2, the embodiment of the utility model discloses a portable soil enzyme activity detection device, include: a dissolving bottle 1 and a cover body 2;
the dissolving bottle 1 is a transparent container with an opening at the top; a sieve plate 11 is fixed in the middle of the dissolving bottle 1 in the radial direction; the sieve plate 11 divides the interior of the dissolving bottle 1 into an upper solid chamber 12 and a lower liquid chamber 13; the outer side wall of the dissolving bottle 1 is respectively provided with a scale mark 14 corresponding to the solid chamber 12 and the liquid chamber 13; the side wall of the dissolving bottle 1 is provided with a liquid inlet 15 corresponding to the liquid cavity 13, and the liquid inlet 15 is positioned above the liquid cavity 13 and below the sieve plate 11; the bottom of the dissolving bottle 1 is provided with a liquid outlet 16;
the cover body 2 is detachably connected with the top opening of the dissolving bottle 1; the top of the cover body 2 is provided with a feed inlet 21.
In order to further optimize the above technical solution, the top edge of the sieve plate 11 has a plurality of elastic clamping strips 111; the top surface of the sieve plate 11 is paved with filter paper 3, and the elastic clamping strip 111 compresses the edge of the filter paper 3.
In order to further optimize the above technical solution, the liquid inlet 15, the liquid outlet 16 and the feed inlet 21 are provided with sealing plugs.
In order to further optimize the technical scheme, the dissolving bottle 1 and the cover body 2 are connected through thread fastening.
In order to further optimize the technical scheme, the bottom of the dissolving bottle 1 is fixed with a lower bracket 4; the lower bracket 4 comprises a plurality of lower support rods 41 vertically fixed with the bottom surface of the dissolution bottle 1, and a lower seat ring 42 fixed at the ends of the plurality of lower support rods 41.
In order to further optimize the above technical solution, the length of the liquid outlet 16 from the bottom surface of the dissolution flask 1 is smaller than the length of the lower ring 42 from the bottom surface of the dissolution flask 1.
In order to further optimize the technical scheme, an upper bracket 5 is fixed on the top surface of the cover body 2; the upper bracket 5 includes a plurality of upper support rods 51 fixed perpendicular to the top surface of the lid body 2, and an upper seat ring 52 fixed to the ends of the plurality of upper support rods 51.
In order to further optimize the above technical solution, the length of the feed inlet 21 from the top surface of the cap body 2 is smaller than the length of the upper seat ring 52 from the top surface of the cap body 2.
In order to further optimize the above solution, the maximum scale of the scale markings 14 corresponding to the solid chamber 12 is greater than 30 ml.
In order to further optimize the above solution, the maximum scale of the graduation marks 14 corresponding to the liquid chamber 13 is larger than 30 ml.
The utility model discloses a use method does:
referring to fig. 3, a desired amount of soil sample is added to the solid chamber 12 through the inlet port 21 and a desired amount of buffer is added to the liquid chamber 13 through the inlet port 15.
Referring to fig. 4, the solvent bottle 1 is turned upside down so that the buffer solution penetrates through the sieve plate 11 and the filter paper 3 into the solid chamber 12, mixed with the soil, and left for a period of time to be mixed uniformly, wherein the time is set according to the mixing requirement.
Referring to fig. 5, after the mixing is completed, the solvent bottle 1 is placed right side again, so that the soil dissolving liquid is separated into the liquid chamber 13, and the soil dissolving liquid for the experiment is obtained.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A portable soil enzyme activity detection device, comprising: a dissolving bottle (1) and a cover body (2);
the dissolving bottle (1) is a transparent container with an opening at the top; a sieve plate (11) is radially fixed in the middle of the dissolving bottle (1); the sieve plate (11) divides the interior of the dissolving bottle (1) into an upper solid chamber (12) and a lower liquid chamber (13); the outer side wall of the dissolving bottle (1) is respectively provided with a scale mark (14) corresponding to the solid chamber (12) and the liquid chamber (13); the side wall of the dissolving bottle (1) is provided with a liquid inlet (15) corresponding to the liquid cavity (13), and the liquid inlet (15) is positioned above the liquid cavity (13) and below the sieve plate (11); the bottom of the dissolving bottle (1) is provided with a liquid outlet (16);
the cover body (2) is detachably connected with the top opening of the dissolving bottle (1); the top of the cover body (2) is provided with a feed inlet (21).
2. The portable device for detecting the activity of the soil enzymes in accordance with claim 1, wherein the top surface edge of the sieve plate (11) is provided with a plurality of elastic clamping strips (111); filter paper (3) is laid on the top surface of the sieve plate (11), and the elastic clamping strips (111) compress the edges of the filter paper (3).
3. The portable device for detecting the activity of the soil enzyme according to claim 1, wherein the liquid inlet (15), the liquid outlet (16) and the liquid inlet (21) are provided with sealing plugs.
4. The portable device for detecting the activity of the soil enzymes according to claim 1, wherein the dissolving bottle (1) and the cover body (2) are connected by screw thread fastening.
5. The portable device for detecting the activity of the soil enzymes according to claim 1, wherein a lower bracket (4) is fixed at the bottom of the dissolving bottle (1); the lower support (4) comprises a plurality of lower support rods (41) which are vertically fixed with the bottom surface of the dissolving bottle (1), and a plurality of lower seat rings (42) which are fixed at the ends of the lower support rods (41).
6. The portable device for detecting the activity of the soil enzymes according to claim 5, wherein the length of the liquid outlet (16) from the bottom surface of the dissolution bottle (1) is less than the length of the lower ring (42) from the bottom surface of the dissolution bottle (1).
7. The portable device for detecting the activity of the soil enzyme according to claim 1, wherein an upper bracket (5) is fixed on the top surface of the cover body (2); the upper bracket (5) comprises a plurality of upper supporting rods (51) vertically fixed to the top surface of the cover body (2) and an upper seat ring (52) fixed to the end of each upper supporting rod (51).
8. The portable device for detecting the activity of the soil enzyme according to claim 7, wherein the length of the feed inlet (21) from the top surface of the cover body (2) is less than the length of the upper seat ring (52) from the top surface of the cover body (2).
9. A portable soil enzyme activity detection device as claimed in claim 1, wherein the maximum scale of the graduated scale (14) corresponding to the solid chamber (12) is greater than 30 ml.
10. A portable soil enzyme activity detection device according to claim 1, wherein the maximum scale of the graduated scale (14) corresponding to the liquid chamber (13) is greater than 30 ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021913158.5U CN213240145U (en) | 2020-09-04 | 2020-09-04 | Portable soil enzyme activity detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021913158.5U CN213240145U (en) | 2020-09-04 | 2020-09-04 | Portable soil enzyme activity detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213240145U true CN213240145U (en) | 2021-05-18 |
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ID=75870994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021913158.5U Active CN213240145U (en) | 2020-09-04 | 2020-09-04 | Portable soil enzyme activity detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213240145U (en) |
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2020
- 2020-09-04 CN CN202021913158.5U patent/CN213240145U/en active Active
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