CN213172389U

CN213172389U - Be used for balling up to cultivate enrichment tumour stem cell separator

Info

Publication number: CN213172389U
Application number: CN202020920830.7U
Authority: CN
Inventors: 赵杰; 巫立荣
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-27
Filing date: 2020-05-27
Publication date: 2021-05-11
Anticipated expiration: 2030-05-27

Abstract

The utility model discloses a be used for balling-up to cultivate enrichment tumour stem cell separator belongs to the medical equipment field. The utility model provides a be used for balling-up to cultivate enrichment tumour stem cell separator, is including placing the platform, the center of placing a diapire has set firmly the installation pole, the downside of placing the platform is equipped with the base, the internally mounted of base has driving motor, driving motor's drive shaft extends to the upside of base and installs the down tube, the bottom activity of installation pole is inserted and is established the tip at the down tube, the symmetry is equipped with two mounting brackets, two all rotate the cartridge on the lateral wall of the relative one side of mounting bracket and have the horizontally pivot. The utility model discloses a can be circumference wobbling place the platform and rock the culture dish, can make culture medium flow evenly in the culture dish after adding the culture medium, finally make culture medium evenly distributed in the culture dish, thereby improve the work efficiency when the homogeneity of tumour stem cell growth improves the separation.

Description

Be used for balling up to cultivate enrichment tumour stem cell separator

Technical Field

The utility model relates to the field of medical equipment, more specifically say, relate to a be used for balling-up to cultivate enrichment tumour stem cell separator.

Background

Medical equipment refers to instruments, devices, appliances, materials or other items used alone or in combination in the human body, and also includes required software. The medical equipment is the most basic element of medical treatment, scientific research, teaching, institutions and clinical discipline work, namely professional medical equipment and household medical equipment. The basic condition of medical equipment for continuously improving the technical level of medical science is also an important mark of the degree of modernization, and the medical equipment becomes an important field of modern medical treatment.

There is a growing body of evidence that a tumor cell population is a heterogeneous cell population, wherein only a tumor stem cell subset has the capabilities of unlimited proliferation, self-renewal, multidirectional differentiation and tumor formation, and is the root cause of the occurrence, progression and recurrence of malignant tumors, so that intensive research on the special biological characteristics of tumor stem cells has important scientific significance. At present, chinese patent application No. 200910190868.1 discloses a method for separating tumor stem cells, which separates tumor stem cells from differentiated tumor cells according to the mitochondrial membrane potential of the cells, wherein the cell subset with high mitochondrial membrane potential is rich in tumor stem cells, and the cell subset with low mitochondrial membrane potential is rich in differentiated tumor cells. The culture medium is required to be added for multiple times when the tumor stem cells are separated, and the culture medium is likely to be unevenly distributed when the culture medium is added, so that the growth of the tumor stem cells is uneven, the subsequent separation process is influenced, and the uniformity of the culture medium is required to be improved.

In view of this, we propose a separation device for enrichment of tumor stem cells in spherulitic culture.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved

An object of the utility model is to provide a be used for balling-up to cultivate enrichment tumour stem cell separator to the inhomogeneous problem of distribution when adding the culture medium that proposes in solving above-mentioned background art.

2. Technical scheme

The utility model provides a be used for balling-up to cultivate enrichment tumour stem cell separator, is including placing the platform, the center of placing a diapire has set firmly the installation pole, the downside of placing the platform is equipped with the base, the internally mounted of base has driving motor, driving motor's drive shaft extends to the upside of base and installs the down tube, the tip of establishing at the down tube is inserted in the bottom activity of installation pole, the symmetry is equipped with two mounting brackets on the base, two all rotate the cartridge on the lateral wall of the relative one side of mounting bracket and have the horizontally pivot, two the rotatory frame of cover in the installation pole outside is installed jointly to the relative one end of pivot, install on the inner wall of revolving frame with pivot vertically branch, branch activity is inserted and is established on the installation pole, it is equipped with fixture to place the bench.

Preferably, fixture sets up two fixed frames placing the bench including the symmetry, the spout has been seted up on placing the roof of platform, two the bottom of fixed frame all is equipped with the slider of mutually supporting with the spout, two common screw thread grafting has the control lever on the slider, the one end of control lever extends to the outside of placing the platform and installs the knob.

Preferably, the control rod is composed of two threaded rods with opposite thread directions, and the two threaded rods are respectively inserted on the two sliding blocks in a threaded manner.

Preferably, two bearings are fixedly sleeved on the control rod and are respectively fixedly embedded on the inner walls at two ends of the sliding chute.

Preferably, two limiting grooves are symmetrically formed in the top wall of the placing table, and the bottom ends of the two fixing frames are provided with limiting blocks matched with the limiting grooves.

Preferably, rubber pads are attached to the inner side walls of the two fixing frames.

3. Advantageous effects

Compared with the prior art, the utility model has the advantages of:

1. the utility model can shake the culture dish through the placing platform which can swing in a circle, the culture medium can flow uniformly in the culture dish after being added, and finally the culture medium is uniformly distributed in the culture dish, so that the growth uniformity of the tumor stem cells is improved, and the working efficiency during separation is improved;

2. the mechanism for clamping the culture dish is arranged on the placing table, the culture dish can be fixed quickly, the culture dish can be kept high stability when being shaken, the culture dish can be taken down conveniently, and the operation is simple, efficient and convenient.

Drawings

Fig. 1 is a schematic front view of the overall structure of the present invention;

fig. 2 is a top view of the superstructure of the present invention;

fig. 3 is a top view of the substructure of the present invention;

the reference numbers in the figures illustrate: 1. a placing table; 2. a chute; 3. a fixing frame; 4. a control lever; 5. a limiting groove; 6. a knob; 7. a slider; 8. a bearing; 9. mounting a rod; 10. rotating the frame; 11. a strut; 12. a mounting frame; 13. a rotating shaft; 14. a diagonal bar; 15. a drive motor; 16. a base.

Detailed Description

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "sleeved/connected", "connected", and the like are to be understood in a broad sense, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, the present invention provides a technical solution:

the utility model provides a be used for balling-up to cultivate enrichment tumour stem cell separator, including placing platform 1, the center of placing 1 diapire of platform has set firmly installation pole 9, the downside of placing platform 1 is equipped with base 16, the internally mounted of base 16 has driving motor 15, driving motor 15's drive shaft extends to base 16's upside and installs down tube 14, the tip of establishing at down tube 14 is inserted in the bottom activity of installation pole 9, the symmetry is equipped with two mounting brackets 12 on the base 16, it has horizontally pivot 13 all to rotate the cartridge on the lateral wall of two mounting brackets 12 relative one side, the one end that two pivots 13 are relative is installed jointly and is established the swivel mount 10 in the installation pole 9 outside, install branch 11 with pivot 13 vertically on the inner wall of swivel mount 10, branch 11 activity is inserted and is established on installation pole 9.

Because installation pole 9 movable sleeve is established on branch 11, consequently installation pole 9 can rotate around branch 11, branch 11 fixed mounting is on swivel mount 10, and the pivot 13 at swivel mount 10 accessible both ends rotates, consequently, when the tip that the bottom of installation pole 9 was followed down the down tube 14 rotates according to the circular shape route, installation pole 9 is whole can rotate in a flexible way, it rocks to drive and place platform 1 and be the circumference, place the culture dish and can drive the culture dish and steadily rock on placing platform 1, make inside culture medium evenly distributed, the rotational speed of rational adjustment actuating motor 15 can reduce centrifugal force, avoid the culture medium to spill.

Place and be equipped with fixture on the platform 1, fixture sets up two fixed frames 3 on placing platform 1 including the symmetry, all paste on the inside wall of two fixed frames 3 and be equipped with the rubber pad, improve the centre gripping effect to the culture dish, prevent to skid, place and seted up spout 2 on the roof of platform 1, the bottom of two fixed frames 3 all is equipped with the slider 7 of mutually supporting with spout 2, place the symmetry on the roof of platform 1 and seted up two spacing grooves 5, the bottom of two fixed frames 3 all is equipped with the stopper of mutually supporting with spacing groove 5, through the direction of the fixed frame 3 of stopper restriction, improve the stability of fixed frame 3. Common screw thread grafting has control lever 4 on two sliders 7, and the one end of control lever 4 extends to the outside of placing platform 1 and installs knob 6, and fixed cover is equipped with two bearings 8 on the control lever 4, and two bearings 8 are fixed respectively to be inlayed and are established on the inner wall at 2 both ends of spout, and control lever 4 comprises two threaded rod that screw thread opposite direction, and two threaded rod are the screw thread grafting respectively on two sliders 7.

The working principle is as follows: when placing the culture dish, put two fixed frames 3 with the culture dish between, then rotatory knob 6, control lever 4 rotates, because control lever 4 comprises the different threaded rod of two screw thread directions, consequently control lever 4 can give the screw drive power of two slider 7 equidirectionals not, make two sliders 7 move or move relatively to opposite direction, consequently make two sliders 7 relative and go and can make two fixed frames 3 press from both sides tight culture dish, make the culture dish can not drop when rocking, reverse rotation knob 6 can make two fixed frames 3 reverse migration when taking off the culture dish.

The present invention relates to the prior art of circuits and electronic components and modules, and can be implemented by those skilled in the art without redundancy, and the present invention also does not relate to improvements to the internal structure and method.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a be used for balling-up to cultivate enrichment tumour stem cell separator, is including placing platform (1), its characterized in that: the center of the bottom wall of the placing table (1) is fixedly provided with a mounting rod (9), the lower side of the placing table (1) is provided with a base (16), the inside of the base (16) is provided with a driving motor (15), the driving shaft of the driving motor (15) extends to the upper side of the base (16) and is provided with an inclined rod (14), the bottom end of the mounting rod (9) is movably inserted into the end part of the inclined rod (14), the base (16) is symmetrically provided with two mounting frames (12), the two mounting frames (12) are respectively rotatably inserted into the side wall on one side opposite to each other and are provided with a horizontal rotating shaft (13), one end opposite to each rotating shaft (13) is jointly provided with a rotating frame (10) sleeved outside the mounting rod (9), the inner wall of the rotating frame (10) is provided with a support rod (11) vertical to the rotating shaft (13), and the support rod (11) is movably inserted into the mounting rod (, and a clamping mechanism is arranged on the placing table (1).

2. The device for separating enriched tumor stem cells in spherulization culture according to claim 1, characterized in that: fixture sets up two fixed frames (3) on placing platform (1) including the symmetry, place and seted up spout (2) on the roof of platform (1), two the bottom of fixed frame (3) all is equipped with slider (7) of mutually supporting with spout (2), two common screw thread grafting has control lever (4) on slider (7), the one end of control lever (4) extends to the outside of placing platform (1) and installs knob (6).

3. The device for separating enriched tumor stem cells for spherulization culture according to claim 2, characterized in that: the control rod (4) is composed of two threaded rods with opposite thread directions, and the two threaded rods are respectively inserted on the two sliding blocks (7) in a threaded manner.

4. The device for separating enriched tumor stem cells for spherulization culture according to claim 2, characterized in that: two bearings (8) are fixedly sleeved on the control rod (4), and the two bearings (8) are respectively fixedly embedded on the inner walls at the two ends of the sliding groove (2).

5. The device for separating enriched tumor stem cells for spherulization culture according to claim 2, characterized in that: two limiting grooves (5) are symmetrically formed in the top wall of the placing table (1), and the bottom ends of the fixing frames (3) are provided with limiting blocks matched with the limiting grooves (5).

6. The device for separating enriched tumor stem cells for spherulization culture according to claim 2, characterized in that: rubber pads are attached to the inner side walls of the two fixing frames (3).