CN213172368U - Instrument box capable of quickly separating tissues - Google Patents

Instrument box capable of quickly separating tissues Download PDF

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Publication number
CN213172368U
CN213172368U CN202021231114.4U CN202021231114U CN213172368U CN 213172368 U CN213172368 U CN 213172368U CN 202021231114 U CN202021231114 U CN 202021231114U CN 213172368 U CN213172368 U CN 213172368U
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circuit
central controller
key
electrically connected
buzzer
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朱家源
唐冰
胡志成
曹小玲
王鹏
黄韶斌
徐海琳
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Guangzhou Maishitian Medical Technology Co ltd
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Guangzhou Maishitian Medical Technology Co ltd
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Abstract

The utility model discloses an apparatus box of quickly separating tissue, include: the instrument box body is used for containing dissociation reagent and comprises an opening at the top and an openable cover arranged at the top of the instrument box body; the circuit control unit is installed in the instrument box body and comprises a central controller, a heating circuit, a temperature sensing circuit, a key circuit and a power supply circuit, wherein the central controller is in communication connection with the heating circuit, the temperature sensing circuit and the key circuit respectively, and the power supply circuit is in electric connection with the key circuit, the heating circuit, the temperature sensing circuit and the central controller respectively. The utility model provides an apparatus box of quickly separating tissue can provide invariable operating temperature, can also ensure that the apparatus box body sets for dissociation time.

Description

Instrument box capable of quickly separating tissues
Technical Field
The utility model relates to the field of medical equipment, concretely relates to instrument box of fast digestion separation tissue in order to acquire basement cell suspension.
Background
The wound surface means that the body loses the integrity of the skin due to internal or external factors, and the repair of the wound surface is always a major clinical problem. Clinically, it can be divided into acute and chronic wounds according to time. Acute wound includes serious diseases such as burn and wound, or skin defect formed after surgical excision of scar, skin complexion disease, benign and malignant tumor, etc., and chronic wound includes traumatic ulcer, diabetic ulcer, vascular ulcer, pressure ulcer, radioactive ulcer, tumor ulcer, scar ulcer, etc. Especially, the incidence rate of chronic wounds is on the trend of rising year by year, the disease course is long, the wounds are difficult to heal, the survival rate of skin grafting is low, and the skin function is lost after skin grafting, so that the life quality is reduced, and the social burden is heavy. There are reports in the literature that about 1% of people worldwide suffer from refractory wounds, and about 5% of medical expenses are used for wound repair. According to the American wound healing society data, more than 60% of chronic wounds can not be completely healed after half a year of treatment, and the annual medical cost is extremely high. The cost of chronic wound healing in europe accounts for 2% of the financial expenditure on health and health, and the medical cost of healing wound due to scar hyperplasia is up to $ 120 billion, and the cost is still increasing every year.
Surgical repair is the main treatment method at present, but the problems of traditional skin graft, skin flap graft, stamp skin graft, particle skin graft, reticular skin graft, composite skin graft and the like and emerging tissue engineering skin cannot be effectively solved at present. Especially, after the recovery of serious disease burn patients, scar hyperplasia and sweat gland loss are often caused, the skin loses the original function, and the life quality is low; chronic wounds are delayed and not healed, medical resources are greatly consumed by light patients, and serious patients are seriously harmed to life such as complicated infection, sepsis and the like. The reason for this is that the current various treatment methods and devices for skin repair do not meet the ideal requirements for artificial skin.
The previous study shows that: spraying the substrate cell suspension on the wound surface in the skin grafting can improve the healing rate of the wound surface, improve the later-stage skin healing quality, and even repair part of skin accessory organs, such as sweat glands and the like. At present, the acquisition of the basement cell suspension is mainly realized by digesting the thin skin slices by pancreatin, and the pancreatin is of animal origin, has drug residues and DNA residues, and also has risks of allergy and the like. In addition, the conditions in the cell dissociation process are strict, the treatment time also needs to be strictly controlled, the pollution of microorganisms or other impurities in the air needs to be avoided, the operation is very complicated, and an instrument box for containing a safe and efficient dissociation reagent needs to be developed urgently and is applied to obtaining the basal cell suspension.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a quick digestion separates apparatus box of tissue in order to acquire basal cell suspension, when the tissue that will dissociate needs place this apparatus box in, this apparatus box can provide invariable operating temperature and strictly the accuse dissociation time, obtains required basal cell suspension.
According to an aspect of the present invention, there is provided an instrument cartridge for rapid tissue separation, comprising: the instrument box body is used for containing dissociation reagents and is provided with a circuit control unit arranged in the instrument box body, wherein the instrument box body comprises an opening at the top and an openable cover arranged at the top of the instrument box body;
the circuit control unit comprises a central controller, a heating circuit, a temperature sensing circuit, a key circuit and a power supply circuit, wherein the central controller is respectively in communication connection with the heating circuit, the temperature sensing circuit and the key circuit, and the power supply circuit is respectively in electric connection with the key circuit, the heating circuit, the temperature sensing circuit and the central controller.
Therefore, when the key circuit senses an external operation, a primary control signal is generated and transmitted to the central controller, and starting is realized; the temperature sensing circuit senses the temperature of the dissociation reagent in the instrument box body so as to generate a first secondary control signal and transmit the first secondary control signal to the central controller; the central controller processes the first secondary control signal and compares the processed result with a preset temperature value to generate a second secondary control signal which is transmitted to the heating circuit; the heating circuit generates/stops generating heat based on the second secondary control signal; the power supply circuit supplies power to the central controller, the heating circuit and the temperature sensing circuit.
The utility model provides an apparatus box of quick separation tissue can provide invariable operating temperature, the temperature of real-time supervision dissociation reagent to compare and then through heating circuit's heat production or stop the heat production automatic adjustment dissociation reagent's temperature based on real-time supervision's temperature value and settlement temperature, make things convenient for the built-in dissociation reagent of box body to exert the biggest dissociation activity, ensure that the dissociation reagent accomplishes the tissue separation fast, acquire stratum basale cell, and then make into the cell suspension; the kit is packaged with the dissociation reagent, and can be sterilized and disinfected separately for use, and the dissociation reagent is added into the kit in a sterile environment, and can be used for dissociating tissue.
In some embodiments, the circuit control unit may further include a buzzer circuit connected with the power supply circuit; the key circuit may further include a time key for setting an operation time and a temperature key for setting an operation temperature,
the working time is calculated from the first time when the set working temperature is reached, when the working time reaches the set working time, the central controller generates a third secondary control signal and transmits the third secondary control signal to the buzzer circuit, and the buzzer vibrates;
when the working temperature reaches the set working temperature, the heating circuit stops generating heat; when the working temperature does not reach the set working temperature, the heating circuit generates heat. From this, can remind the user to organize the separation process to end based on the vibration that bee calling organ produced, avoid making dissociation time overlength because of neglecting to take out the tissue, destroy the integrality of basement cell, whole operation process is more intelligent. In addition, the working temperature during dissociation can be set through the instrument box, so that the high activity of the dissociation reagent is ensured, and cells are not inactivated due to overhigh working temperature during dissociation or can not be fully dissociated due to overlow working temperature.
In some embodiments, the instrument cartridge further includes a pair of ear-type handles disposed on either side of the cartridge. Therefore, the ear-shaped handles on the two sides of the box body enable a user to conveniently lift the instrument box body by hands.
In some embodiments, a display panel may be further disposed on the instrument box body, and the display panel is used for displaying the working time and the working temperature. Therefore, the working time and the working temperature are displayed through the display panel, the working temperature of the dissociation reagent and the dissociation time of the dissociation reagent are monitored in real time, the deviation of the working temperature of the dissociation reagent from a set value (namely the highest active temperature) or the shortage or the prolonging of the dissociation time are effectively prevented, and the monitoring is convenient.
In some embodiments, the dissociating agent may be tryptleTMExpress dissociation reagent. The reagent is a non-animal-derived recombinase, can efficiently dissociate tissues, has mild action on cells, can protect cell surface protein, and has small influence on cell functions.
In some embodiments, the heating circuit comprises a third mosfet and a heating plate; an output pin of the central controller is electrically connected with a grid electrode of a third MOS field effect transistor, a drain electrode of the third MOS field effect transistor is electrically connected with a first pin of the pin header J3, and a source electrode of the third MOS field effect transistor is grounded; the power supply VCC is electrically connected with a second pin of the pin header J3 so as to supply power for the heating circuit and the temperature sensing circuit; the third pin of the pin header J3 is electrically connected with the central controller, so that the temperature signal output by the temperature sensing circuit is transmitted to the central controller; the needle row seat J4 is butted with a needle row seat J3; the heating plate is connected between the first pin and the fourth pin of the pin header J4, and the temperature sensing circuit is connected between the second pin and the third pin of the pin header J4. Therefore, the central controller controls the third MOS field effect transistor to be switched on/off, the central controller controls the heating circuit to work or stop working, and the working temperature of the dissociation reagent can be controlled more accurately and intelligently through the set program.
In some embodiments, the power circuit may further include a voltage regulator and a battery, the key circuit and the voltage regulator are connected in series, and the battery supplies power to the central controller, the heating circuit, the temperature sensing circuit and the buzzer circuit through the voltage regulator. Therefore, through the voltage stabilizing effect of the voltage stabilizer, ripple voltage in the circuit or common-mode signals generated by self-inductance of the electric circuit are removed, and the effect of stabilizing working voltage is provided for the working circuit; on the other hand, the whole opening/closing machine of the instrument box can be controlled through the key circuit, so that an operator can conveniently control the instrument box.
In some embodiments, the key circuit may include an on-key, a first transistor, and a second mosfet;
one end of the power-on key is electrically connected with the central controller, and the other end of the power-on key is electrically connected with the base electrode of the first triode;
the anode/cathode of the battery is respectively and electrically connected with the emitter of the first triode and the drain of the second MOS field effect transistor, and the cathode/anode of the battery is grounded;
the grid electrode of the second MOS field effect transistor is respectively and electrically connected with the central controller and the collector electrode of the first triode, and the source electrode of the second MOS field effect transistor is electrically connected with the voltage stabilizer;
when the starting key is pressed, the power link between the battery and the central controller and the voltage stabilizer is instantly conducted, and the central controller generates a fourth secondary control signal and transmits the fourth secondary control signal to the second MOS field effect transistor, so that the battery and the voltage stabilizer are electrically conducted. Therefore, the first triode and the second MOS field effect transistor both play a switching role to control the initial conduction between the battery and the central controller, when a power-on key is pressed, the electric power conduction between the battery and the central controller is realized, the central controller outputs a fourth control signal to the grid electrode of the second MOS field effect transistor to control the conduction/disconnection of the second MOS field effect transistor, and further the working state of the instrument box is controlled by the central controller, when the power-on key is released, the battery is continuously electrically conducted with the voltage stabilizer, and further the central controller, the heating circuit, the temperature sensing circuit and the buzzer circuit are powered.
In some embodiments, the key circuit may further include a sixth diode, and the sixth diode is used for realizing unidirectional power conduction between the battery and the second mosfet. Therefore, when the anode of the battery is electrically connected with the drain electrode of the second MOS field effect transistor, only the current is allowed to flow from the battery end to the voltage stabilizer end, and the current is not allowed to flow reversely; when the negative electrode of the battery is electrically connected with the drain electrode of the second MOS field effect transistor, only the current is allowed to flow from the voltage stabilizer end to the battery end, but the current is not allowed to flow reversely, so that the damage to the circuit of the whole instrument box caused by the reverse connection of the battery is effectively avoided.
In some embodiments, the buzzer circuit may include a buzzer and a fourth transistor, one end of the buzzer is electrically connected to the voltage regulator, the other end of the buzzer is electrically connected to a collector of the fourth transistor, a base of the fourth transistor is electrically connected to the central controller, and an emitter of the fourth transistor is grounded. Therefore, the fourth triode plays a role of a switch, whether the buzzer circuit is conducted or not is controlled through the central controller, and whether the buzzer works or not is further controlled, so that the whole instrument box is more intelligent.
In some embodiments, the central controller may include a single-chip microcomputer, model PIC16F 676. The PIC16F676 singlechip uses an internal crystal oscillator, and an EEPROM (electrically erasable programmable read-Only memory) is internally provided with a power-down storage memory to record the use condition, so that the PIC16F676 singlechip is low in cost, high in cost performance and low in power consumption.
In some embodiments, the heating pad may be an FPC heating soft pad, and the temperature sensing circuit includes an NTC10K temperature sensor. The FPC heating soft board has the characteristics of high wiring density, good heat dissipation performance, light weight, thin thickness and the like, and is suitable for being used inside an instrument box for heating dissociation reagents; the NTC10K temperature sensor is a thermistor sensor, has moisture resistance, good insulation, high reliability, small time constant and small volume, and is suitable for temperature detection in medical instruments.
In some embodiments, the voltage regulator may be an HT7650 type voltage regulator. The HT7650 type voltage stabilizer is an ultra-low power consumption voltage stabilizer, outputs +5v voltage, and has stable performance, high compatibility and high anti-interference capability.
Drawings
FIG. 1 is a schematic diagram of a circuit module connection of an instrument cassette for rapid tissue separation according to the present invention;
FIG. 2 is a schematic view of an overall structure of an instrument box according to an embodiment of the present invention;
FIG. 3 is a circuit diagram of the central controller connected to the heating circuit and the temperature sensing circuit;
FIG. 4 is a circuit diagram of a power circuit and a key circuit;
fig. 5 is a circuit diagram of a buzzer circuit.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Fig. 1 to 5 schematically show a tissue rapid separation cartridge according to an embodiment of the present invention.
The instrument box comprises: dissociating reagent (here, dissociating reagent may be pancreatin mixed liquor system or TrypLE)TMExpress dissociation agent, preferably TrypLETMExpress dissociation reagent, TrypLETMExpress dissociation reagent is purchased from Invitrogen company), an instrument box body 1 for containing the dissociation reagent, and a circuit control unit installed in the instrument box body 1, wherein the circuit control unit comprises a central controller S1 (the central controller S1 may comprise a single chip microcomputer, for example, a single chip microcomputer with the model number of PIC16F 676), a heating circuit S2, a temperature sensing circuit S3, a key circuit S4 and a power circuit S5, and the power circuit S5 is electrically connected with the key circuit S4, the heating circuit S2, the temperature sensing circuit S3 and the central controller S1 respectively to supply power to the key circuit, the heating circuit S2, the temperature sensing circuit S3 and the central controller S1; the key circuit S4 is connected to the central controller S1, and the key circuit S4 canExternal operation can be induced for realizing man-machine interaction; the heating circuit S2 is in communication connection with the central controller S1, and the heating circuit S2 generates heat energy or stops generating heat energy under the control of the central controller S1, thereby stabilizing the temperature; the temperature sensing circuit S3 is communicatively connected to the central controller S1, and the temperature sensing circuit S3 is used to detect temperature.
The central controller S1 controls whether the heating circuit S2 is operated and the operation duration based on the signals received from the key circuit S4 and the temperature sensing circuit S3, as follows. When the key circuit S4 senses an external operation, it generates a primary control signal and transmits it to the central controller S1, and the central controller S1 controls the instrument box to start (e.g., the external operation may be pressing the power key; the primary control signal is an electrical signal; e.g., when the power key is pressed, the central controller S1 is connected to the power circuit, and the power circuit sends V to the central controller S1signalThe central controller S1 controls the instrument box to start up when the electrical signal is equal to the power voltage), and at this time, the power circuit S5 energizes the central controller S1, the temperature sensing circuit S3 starts to work, but the heating circuit S2 does not work, so as to achieve the effect of preparing for starting up.
The temperature sensing circuit S3 senses the temperature of the dissociation reagent in the instrument cartridge 1 and generates a first secondary control signal (for example, the first secondary control signal is a voltage signal, and may be, for example, a voltage across a thermistor, and includes a positive temperature coefficient thermistor (PTC) and a negative temperature coefficient thermistor (NTC), where the resistance of the thermistor is in a linear relationship with the temperature, and different resistances correspond to different temperatures, so as to output a voltage signal corresponding to a specific temperature, thereby achieving an effect of linearly matching the output voltage signal with the temperature value. The temperature sensing circuit S3 transmits the first secondary control signal to the central controller S1, and the central controller S1 processes the first secondary control signal and compares the processed result with the preset temperature value (here, the voltage of the first secondary control signal may be compared with the voltage corresponding to the converted preset temperature value), so as to generate a second secondary control signal and transmit the second secondary control signal to the heating circuit S2, and control the heating circuit S2 to stop generating heat. For example, the second secondary control signal is a pulse voltage signal, and the pulse voltage signal is transmitted to the MOS field effect transistor between the power supply circuit S5 and the heater plate to control on/off between the heater plate and the power supply circuit S5, thereby controlling the heater circuit S2 to generate/stop generating heat. In the present embodiment, the frequency of the pulse voltage signal determines the heating operation power of the heating circuit S2, and when no pulse voltage signal is output, the heating is stopped. In this embodiment, when the difference between the operating temperature and the preset operating temperature is large, for example, the operating temperature is lower than the preset operating temperature by more than 5 ℃, the frequency of the pulse voltage signal is large; when the working temperature is lower than the preset working temperature by within 5 ℃, the frequency of the pulse voltage signal is smaller. In the present embodiment, when the operating temperature reaches or exceeds the set operating temperature, the heating circuit S2 stops generating heat; when the operating temperature does not reach the set operating temperature, the heating circuit S2 continues to generate heat.
When in use, the instrument box body 1 and the dissociation reagent TrypLE are separatedTMExpress is sterilized and disinfected, and after completion, the dissociation reagent TrypLE is sterilized in a sterile environmentTMExpress is added to the instrument cartridge 1, at which time, a tissue to be dissociated, which may be, for example, a skin tissue (here, an artificially cultured skin tissue may be used), is placed in the instrument cartridge 1, and an on-key is pressed so that the key circuit S4 senses the presence of an external operation, thereby activating the instrument cartridge, operating based on a default operating temperature (which may be, for example, 37 ℃) and operating time (which may be, for example, 15min) of the central controller S1. The temperature sensing circuit S3 senses the temperature of the dissociation reagent in the instrument box body 1 in real time, when the sensed temperature is lower than 32 ℃, the central controller S1 sends a high-frequency pulse electric signal to the heating circuit S2 and controls the heating circuit S2 to rapidly generate heat; when the sensed temperature is equal to or higher than 37 ℃, the central controller S1 does not send a pulse electric signal to the heating circuit S2, and the heating circuit S2 stops heating; when the sensed temperature is highWhen the temperature is 32 ℃ and lower than 37 ℃, the central controller S1 sends out a low-frequency pulse electric signal to the heating circuit S2, and controls the heating circuit S2 to slowly generate heat; therefore, the working temperature of the dissociation reagent during working is strictly controlled through a real-time feedback regulation mechanism.
In the present embodiment, as shown in fig. 1, the circuit control unit may further include a buzzer circuit S6, and the key circuit S4 may further include a time key for setting an operating time and a temperature key for setting an operating temperature. The power supply circuit S5 is connected with the buzzer circuit S6 and supplies power to the buzzer circuit S6; the buzzer circuit S6 is communicatively connected to the central controller S1, and the buzzer circuit S6 implements an alarm under the control of the central controller S1.
When the working time reaches the set working time, the central controller S1 generates a third secondary control signal and transmits the third secondary control signal to the buzzer circuit S6, and the buzzer vibrates to realize alarming. For example, the third secondary control signal may be an electrical signal, such as a high level signal, and when the high level signal is transmitted to a transistor as a switch of the buzzer circuit S6, the buzzer circuit S6 is electrically conducted with the power circuit S5 to generate vibration. In this embodiment, the operation time is calculated from the time when the dissociation reagent reaches the set operation temperature for the first time, and when the operation time reaches the set operation time, the central controller S1 stops outputting the pulse electric signal (i.e., stops heating). At this time, the skin tissue is taken out, the epidermis is separated, the basal layer cells are obtained, and the cells can be added into normal saline or nutrient solution to prepare cell suspension.
In the present embodiment, as shown in fig. 2, the instrument case 1 includes a top opening, an openable cover 11 disposed on the top of the case 1, and a pair of ear-shaped handles 12 disposed on two sides of the case, and the top of the instrument case 1 is sealed by the openable cover 11 during use to keep the temperature. Ear type handles 12 on both sides of the case body allow a user to conveniently lift the instrument case with the hand. In this embodiment, the openable cover 11 slides relative to the instrument case 1 through a guide rail, and a handle is provided on the side of the openable cover 11 extending out of the instrument case 1 to facilitate the insertion/extraction of the openable cover 11 into/from the instrument case 1. In this embodiment, the openable cover 11 can be pulled out from the front end of the opening, and a groove is formed at the rear end of the opening, so that the dissociation reagent can be poured conveniently. In this embodiment, the openable cover 11 can also be hinged to the side of the top opening of the box 1 by a fixing bolt, and the openable cover 11 can be opened/closed.
In the embodiment, as shown in fig. 2, the front of the instrument box body 1 is further provided with a display panel 13, the display panel 13 can display the set working time, the completed working time, the remaining working time, the set working temperature, the current working temperature and the like, and the instrument box can be operated according to the data displayed by the display panel 13 during use. In this embodiment, the display panel 13 is a touch display screen, and the time key and the temperature key are both disposed on the display panel 13 and are touch keys, so that the operating time and the operating temperature can be set through the display panel 13. In this embodiment, the front surface of the instrument box 1 is further provided with a power-on key 14, when the power-on key 14 is pressed before use, the instrument box is powered on, and the display panel 13 emits light when the instrument box is powered on.
In the present embodiment, as shown in fig. 3, the heating circuit includes a third MOS field effect transistor Q3 and a heating plate; an output pin of the central controller S1 is electrically connected with the gate of the third MOS field effect transistor Q3, the drain of the third MOS field effect transistor Q3 is electrically connected with the first pin of the pin header J3, and the source of the third MOS field effect transistor is grounded; the power source VCC is electrically connected with the second pin of the header J3 to supply power to the heating circuit S2 and the temperature sensing circuit S3; the third pin of the pin header J3 is electrically connected with the central controller S1, so as to transmit the temperature signal output by the temperature sensing circuit S3 to the central controller S1; the needle arranging seat J4 is butted with the needle arranging seat J3; a heating plate is connected between the first pin and the fourth pin of the pin header J4, and a temperature sensing circuit S3 is connected between the second pin and the third pin of the pin header J4.
The central controller S1 displays the temperature of the dissociation reagent in the instrument cartridge 1 through the display panel 13. When the heating circuit is used, the central controller S1 controls whether the third MOS fet Q3 is turned on or off and the magnitude of the current passing through the third MOS fet Q3 by outputting a high/low frequency pulse signal or not, thereby controlling the operation or non-operation of the heating circuit S2 and the heating power during operation.
In the present embodiment, as shown in fig. 3, the heating circuit S2 includes an FPC (flexible printed circuit) heating flexible board, and the temperature sensing circuit S3 includes a ntc thermistor temperature sensor having a resistance value of 10K Ω. In this embodiment, the Power circuit S5 further includes a voltage regulator U1(HT7650 type voltage regulator) and a battery, the key circuit S4 and the voltage regulator U1 are sequentially connected in series, and the battery is connected to the voltage regulator U1 when the key circuit S4 is turned on (i.e., when the Power-on key Power _ on _ test is pressed, corresponding to the Power-on key 14 in fig. 2). The battery supplies power to the central controller S1, the heating circuit S2, the temperature sensing circuit S3 and the buzzer circuit S6 through the regulator U1 (i.e., the VCC output terminal of the regulator U1 outputs a stable +5V voltage). In a specific embodiment, the battery can be replaced by other external direct current power supplies or an integrated alternating current power supply, and the same power supply effect is achieved.
In the present embodiment, as shown in fig. 4, the key circuit S4 includes an on-key 14, a first transistor Q1, and a second MOS fet Q2; one end of the power-on key 14 is electrically connected with the central controller S1, and the other end of the power-on key 14 is connected with the base of a first triode Q1(PNP triode); the positive electrode of the battery is respectively and electrically connected with the emitter of the first triode Q1 and the drain of the second MOS field effect transistor Q2, the negative electrode of the battery is grounded (in other embodiments, the negative electrode of the battery is respectively and electrically connected with the emitter of the first triode Q1 and the drain of the second MOS field effect transistor Q2, and the positive electrode of the battery is grounded, so that the same power supply effect is achieved); the grid electrode of the second MOS field effect transistor Q2 is respectively and electrically connected with the central controller S1 and the collector electrode of the first triode Q1, and the source electrode of the second MOS field effect transistor Q2 is electrically connected with the voltage stabilizer U1; when the power key 14 is pressed down, the switch of the first triode Q1 is instantly turned on, so that the gate of the second MOS fet Q2 is at a high level, the battery is electrically connected with the central controller S1 and the voltage regulator U1, and at this time, the central controller S1 generates a fourth secondary control signal (where the fourth secondary control signal is a high level signal, and the high level signal is transmitted to the gate of the second MOS fet Q2, so that the battery is electrically connected with the voltage regulator U1), so that when the power key 14 is released, the first triode Q1 is turned off, and the central controller S1 outputs the fourth secondary control signal to the gate of the second MOS fet Q2, so that the battery is electrically connected with the voltage regulator U1, and power is continuously supplied to the central controller S1, the heating circuit S2, the temperature sensing circuit S3 and the buzzer circuit S6.
In this embodiment, as shown in fig. 4, the key circuit S4 further includes a sixth diode D6, an anode of the sixth diode D6 is electrically connected to the battery, and a cathode of the sixth diode D6 is electrically connected to a drain of the second mosfet, so that current is only allowed to flow from the battery terminal to the second mosfet (i.e., the battery is forward biased to the second mosfet), and damage to the instrument box caused by reverse connection of the power source can be effectively prevented. In other embodiments, when the negative electrode of the battery is electrically connected to the emitter of the first transistor Q1 and the drain of the second mosfet Q2, respectively, and the positive electrode of the battery is grounded, the cathode of the sixth diode D6 is electrically connected to the battery, and the anode of the sixth diode D6 is electrically connected to the drain of the second mosfet, thereby only allowing current to flow from the second mosfet terminal to the battery terminal (i.e., the battery is reverse biased to the second mosfet), which has the same power supply effect.
In this embodiment, as shown in fig. 5, the BUZZER circuit S6 may include a BUZZER and a fourth transistor Q4 (NPN-type transistor), wherein an anode of the BUZZER is electrically connected to the voltage regulator U1, a cathode of the BUZZER is electrically connected to a collector of the fourth transistor Q4, a base of the fourth transistor Q4 is electrically connected to the central controller S1, and an emitter of the fourth transistor Q4 is grounded. When the tissue dissociation process is finished, the central controller S1 outputs a high level and transmits the high level to the base electrode of the fourth triode Q4, so that a power link between the voltage stabilizer U1 and the BUZZER BUZZER-the ground is conducted, the BUZZER buZZER works to emit dripping sound, and a user is reminded of finishing the tissue dissociation process; at other times, the central controller S1 outputs a low signal, and the buzzer circuit S6 is not turned on and does not operate.
In this embodiment, the cathode of the BUZZER is electrically connected to the anode of the seventh diode D7, and the anode of the BUZZER is electrically connected to the cathode of the seventh diode D7, so that the self-inductance current generated by the BUZZER is looped through the seventh diode D7, thereby preventing the sudden power failure from generating a large induced electromotive force and damaging the electronic components.
What has been described above are only some embodiments of the invention. For those skilled in the art, without departing from the inventive concept, several modifications and improvements can be made, which are within the scope of the invention.

Claims (10)

1. An instrument cassette for rapid tissue separation, comprising:
the device comprises an instrument box body (1) for containing a dissociation reagent, wherein the instrument box body (1) comprises a top opening and an openable cover body (11) arranged at the top of the instrument box body (1);
a circuit control unit installed in the instrument cartridge (1), the circuit control unit including a central controller (S1), a heating circuit (S2), a temperature sensing circuit (S3), a key circuit (S4), and a power supply circuit (S5),
the central controller (S1) is respectively connected with the heating circuit (S2), the temperature sensing circuit (S3) and the key circuit (S4) in a communication way,
the power supply circuit (S5) is electrically connected with the key circuit (S4), the heating circuit (S2), the temperature sensing circuit (S3) and the central controller (S1), respectively.
2. The cartridge according to claim 1, wherein the circuit control unit further comprises a buzzer circuit (S6), the buzzer circuit (S6) being connected with a power supply circuit (S5); the key circuit (S4) further includes a time key for setting an operating time and a temperature key for setting an operating temperature.
3. The instrument cartridge according to claim 2, characterized in that the instrument cartridge body (1) is further provided with a display panel (13) for displaying working time and working temperature.
4. The instrument cartridge according to claim 3, wherein the instrument cartridge body (1) further comprises a pair of ear type handles (12) provided on both sides of the instrument cartridge body (1).
5. The instrument cassette of any of claims 1 to 4, wherein the heating circuit comprises a third MOS field effect transistor (Q3) and a heating plate; an output pin of the central controller (S1) is electrically connected with the grid electrode of the third MOS field effect transistor (Q3), the drain electrode of the third MOS field effect transistor (Q3) is electrically connected with the first pin of the pin header J3, and the source electrode of the third MOS field effect transistor is grounded; the power supply VCC is electrically connected with the second pin of the pin header J3, the third pin of the pin header J3 is electrically connected with the central controller (S1), and the temperature signal output by the temperature sensing circuit (S3) is transmitted to the central controller (S1); the needle arranging seat J4 is butted with the needle arranging seat J3; the heating plate is connected between the first pin and the fourth pin of header J4, and the temperature sensing circuit is connected between the second pin and the third pin of header J4 (S3).
6. The instrument box of any one of claims 1 to 4, wherein the power circuit (S5) further comprises a voltage regulator (U1) and a battery, the key circuit (S4) and the voltage regulator (U1) being connected in series in sequence, the battery supplying power to the central controller (S1), the heating circuit (S2), the temperature sensing circuit (S3) and the buzzer circuit (S6) through the voltage regulator (U1).
7. The instrument box of claim 6, wherein the key circuit (S4) includes an on-key (14), a first transistor (Q1), and a second MOS FET (Q2);
one end of the start-up key (14) is electrically connected with the central controller (S1), and the other end of the start-up key (14) is electrically connected with the base electrode of the first triode (Q1);
the positive electrode/negative electrode of the battery is respectively and electrically connected with the emitter of the first triode (Q1) and the drain of the second MOS field effect transistor (Q2), and the negative electrode/positive electrode of the battery is grounded;
the grid electrode of the second MOS field effect transistor (Q2) is respectively and electrically connected with the central controller (S1) and the collector electrode of the first triode (Q1), and the source electrode of the second MOS field effect transistor (Q2) is electrically connected with the voltage stabilizer (U1);
when the set-top key (14) is pressed, a power link between the battery and the central controller (S1) and the voltage stabilizer (U1) is conducted instantly, the central controller (S1) generates a fourth secondary control signal and transmits the fourth secondary control signal to the second MOS field effect transistor (Q2), and power conduction between the battery and the voltage stabilizer (U1) is achieved.
8. The instrument box according to claim 7, wherein the key circuit (S4) further comprises a sixth diode (D6) for enabling power unidirectional conduction between the battery and the second MOS FET.
9. The cartridge according to claim 8, characterized in that the BUZZER circuit (S6) comprises a BUZZER (BUZZER) and a fourth transistor (Q4), one end of the BUZZER (BUZZER) being electrically connected to the regulator (U1), the other end of the BUZZER (BUZZER) being electrically connected to the collector of the fourth transistor (Q4), the base of the fourth transistor (Q4) being electrically connected to the central controller (S1), the emitter of the fourth transistor (Q4) being grounded.
10. The instrument cartridge according to any of the claims 7 to 9, characterized in that the central controller (S1) comprises a single-chip microcomputer, the single-chip microcomputer being of the type PIC16F 676.
CN202021231114.4U 2020-06-29 2020-06-29 Instrument box capable of quickly separating tissues Active CN213172368U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021231114.4U CN213172368U (en) 2020-06-29 2020-06-29 Instrument box capable of quickly separating tissues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021231114.4U CN213172368U (en) 2020-06-29 2020-06-29 Instrument box capable of quickly separating tissues

Publications (1)

Publication Number Publication Date
CN213172368U true CN213172368U (en) 2021-05-11

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021231114.4U Active CN213172368U (en) 2020-06-29 2020-06-29 Instrument box capable of quickly separating tissues

Country Status (1)

Country Link
CN (1) CN213172368U (en)

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