CN213139530U - Novel human scalp hair follicle internal sulfotransferase activity detection kit - Google Patents
Novel human scalp hair follicle internal sulfotransferase activity detection kit Download PDFInfo
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- CN213139530U CN213139530U CN202021428041.8U CN202021428041U CN213139530U CN 213139530 U CN213139530 U CN 213139530U CN 202021428041 U CN202021428041 U CN 202021428041U CN 213139530 U CN213139530 U CN 213139530U
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Abstract
The utility model discloses a novel test kit for the activity of sulfotransferase in hair follicles of human scalp, which comprises a box body and a cover body; the lower layer container is placed in the box body and comprises a container body and a plurality of test tube grooves arranged on the container body, the openings of the test tube grooves are upward, a test tube is inserted into each test tube groove, and each test tube contains a liquid reagent; the upper reaction plate is placed in the box body and positioned above the lower container, the upper reaction plate comprises a reaction plate body and a reaction plate cover body, the reaction plate body is provided with a plurality of reagent holes and a reaction hole, each reagent hole is used for containing a solid reagent, a reagent hole cover is arranged on the inner side of the reaction plate cover body corresponding to each reagent hole respectively, a reaction hole cover is arranged corresponding to each reaction hole, when the reaction plate body is plugged by the reaction plate cover body, the reagent hole covers are in sealing connection with the corresponding reagent holes, and the reaction hole covers are in sealing connection with the reaction holes. The utility model discloses can deposit liquid and solid-state reagent subregion, be convenient for transport under different storage conditions.
Description
Technical Field
The utility model relates to a life science technical field, concretely relates to novel detection kit for sulfotransferase activity in human head fur follicle.
Background
Androgenic alopecia (AGA) is one of the most common skin diseases in the world, and afflicts over 1 million adult males in our country. Minoxidil is a gold standard treatment for androgenetic alopecia, and its mechanism of action is that Minoxidil binds to a substance named "Sulfotransferase (SUL)" in the scalp and hair follicles to form Minoxidil sulfate (Minoxidil sulfate), which is a component having a critical effect on hair growth, and changes the growth cycle of the hair follicles, converting the resting-stage hair follicles into a growing stage.
The heat-resistant sulfotransferase 1/cytoplasmic sulfotransferase family 1A member 1 (SURT 1A1) is used as a key enzyme for minoxidil to exert biological activity, the detection of the content and the activity of the enzyme can help us to know the conversion condition of minoxidil after the minoxidil is applied, and the SURT 1A1 in hair follicles is obviously and positively correlated with the curative effect of local application minoxidil, so that the enzyme can be used as an ideal biomarker for predicting the curative effect of minoxidil.
Most of the existing methods for detecting heat-resistant sulfotransferase 1 in the market at present are enzyme-linked immunosorbent assay (Elisa) and colorimetric method. The enzyme-linked immunosorbent assay standard is the concentration of thermostable sulfotransferase 1, cannot directly indicate the enzymatic activity, has excessive extract components and complex process, and is generally used for laboratory research of' returning transforming SULT1A1, a porous biological identifier in nutritional genetic testing rate model of Rapid HAPE indicating Life Sci, 2019Jul 15; 229:132-138.". The colorimetric method is a more efficient, simple and convenient method for measuring the activity of heat-resistant sulfotransferase 1, namely' chemical nanoparticles for targeting and stabilizing minor sulfate delivery to microorganisms [ J ]. International Journal of Biological macromolecules, 2015 Apr; 75:225-9 ", however, no clinically applicable kit for detecting the activity of human thermotolerant sulfotransferase 1 in hair follicles is available.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel human scalp hair follicle endotheca sulfotransferase activity detect reagent box can deposit liquid and solid-state reagent subregion, is convenient for transport under different storage conditions.
To achieve the purpose, the utility model adopts the following technical proposal:
the utility model provides a novel human scalp hair follicle endotheca sulfotransferase activity detect reagent box, including the box body with be used for the selective shutoff lid of box body one side opening still includes:
the lower layer container is placed in the box body and comprises a container body and a plurality of test tube grooves which are arranged on the container body at intervals, the openings of the test tube grooves are upward, a test tube is inserted into each test tube groove, and each test tube is used for containing a liquid reagent;
the upper reaction plate is placed in the box body and positioned above the lower container, the upper reaction plate comprises a reaction plate body and a reaction plate cover body, the reaction plate body selectively blocks the opening end of the reaction plate body, a plurality of reagent holes with upward openings and a reaction hole are formed in the reaction plate body at intervals, each reagent hole is used for containing a solid reagent, a reagent hole cover is arranged on the inner side of the reaction plate cover body corresponding to each reagent hole, a reaction hole cover is arranged corresponding to the reaction hole, when the reaction plate body is blocked by the reaction plate cover body, the reagent hole covers are in sealing connection with the corresponding reagent holes, and the reaction hole covers are in sealing connection with the reaction holes.
As a preferred embodiment of the novel test kit for detecting the activity of sulfotransferase in hair follicles of human scalp, the test tubes comprise a first test tube for containing a phosphate buffer solution, a second test tube for containing a Tris-HCl buffer solution and a third test tube for containing a magnesium chloride hexahydrate solution.
As a preferable mode of the novel test kit for detecting the activity of the sulfotransferase in the hair follicle of the human scalp, the reagent holes comprise a first reagent hole for containing minoxidil powder, a second reagent hole for containing potassium p-nitrophenyl sulfate crystals and a third reagent hole for containing adenosine 3 ', 5' diphosphate, and the reagent hole cover comprises a first reagent hole cover capable of being hermetically connected with the first reagent hole, a second reagent hole cover capable of being hermetically connected with the second reagent hole and a third reagent hole cover capable of being hermetically connected with the third reagent hole.
As a preferable scheme of the novel test kit for detecting the activity of the human scalp intrafollicular sulfotransferase, the reagent hole and the reaction hole are both circular, and the diameter of the reaction hole is larger than that of the reagent hole.
As a preferable scheme of the novel test kit for detecting the activity of the sulfotransferase in the hair follicles of the human scalp, the reagent hole cover and the reaction hole cover are respectively provided with a sealing ring.
As a preferable embodiment of the novel test kit for detecting the activity of the human scalp intrafollicular sulfotransferase, the reaction plate cover body can be hermetically connected with the reaction plate body.
The utility model has the advantages that: the utility model discloses a sulfotransferase activity detect reagent box in people's scalp hair bag can deposit liquid and solid-state reagent subregion, is convenient for transport under different storage conditions.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below. It is obvious that the drawings described below are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be obtained from these drawings without inventive effort.
Fig. 1 is a schematic exploded view of a novel test kit for detecting the activity of sulfotransferase in hair follicles in a human scalp according to an embodiment of the present invention.
In the figure:
1. a box body; 2. a cover body; 3. a lower layer container; 31. a container body; 32. a test tube groove; 33. a test tube; 331. a first test tube; 332. a second test tube; 333. a third test tube; 4. an upper reaction plate; 41. a reaction plate body; 42. a reaction plate cover body; 43. A reagent well; 431. a first reagent well; 432. a second reagent well; 433. a third reagent well; 44. a reaction well; 45. a reagent well cover; 451. a first reagent well cover; 452. a second reagent well cover; 453. a third reagent well cover; 46. reaction hole covers.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.
Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "inner", "outer", etc. are used to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not indicated or implied that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are used only for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms will be understood by those skilled in the art according to the specific circumstances.
In the description of the present invention, unless otherwise explicitly specified or limited, the term "connected" or the like, if appearing to indicate a connection relationship between the components, is to be understood broadly, for example, as being either a fixed connection, a detachable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through one or more other components or may be in an interactive relationship with one another. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
This embodiment provides a novel test kit for detecting the activity of sulfotransferase in hair follicles of human scalp, which comprises a case 1 and a cover 2 for selectively sealing an opening portion on one side of the case 1, and further comprises:
the lower container 3 is placed in the box body 1, the lower container 3 comprises a container body 31 and a plurality of test tube grooves 32 which are arranged on the container body 31 at intervals, the openings of the test tube grooves 32 are upward, a test tube 33 is inserted into each test tube groove 32, and each test tube 33 is used for containing a liquid reagent;
the upper reaction plate 4 is placed in the box body 1 and located above the lower container 3, the upper reaction plate 4 includes a reaction plate body 41 and a reaction plate cover 42 for selectively blocking the upper end of the reaction plate body 41, the reaction plate body 41 is provided with a plurality of reagent holes 43 with upward openings and a reaction hole 44 at intervals, each reagent hole 43 is used for accommodating a solid reagent, a reagent hole cover 45 is respectively provided on the inner side of the reaction plate cover 42 corresponding to each reagent hole 43, a reaction hole cover 46 is provided corresponding to the reaction hole 44, when the reaction plate body 41 is blocked by the reaction plate cover 42, the reagent hole cover 45 is hermetically connected with the corresponding reagent hole 43, and the reaction hole cover 46 is hermetically connected with the reaction hole 44.
In this embodiment, each liquid reagent is accommodated in one test tube 33 in the test tube well 32 of the lower container 3, each solid reagent is accommodated in the reagent well 43 of the upper reaction plate 4, and the lower container 3 and the upper reaction plate 4 can be taken out separately, and can be transported under transport conditions such as different temperatures. For example, the lower vessel 3 may be stored and transported at 4 ℃ and the upper reaction plate 4 may be stored and transported at-20 ℃.
Wherein the test tubes 33 comprise a first test tube 331 for containing a phosphate buffer, a second test tube 332 for containing a Tris-HCl buffer, and a third test tube 333 for containing a magnesium chloride hexahydrate solution. The test tubes 33 are respectively labeled, and the three test tubes 33 can be inserted into any one of the test tube slots 32.
Wherein the reagent well 43 comprises a first reagent well 431 for containing minoxidil powder, a second reagent well 432 for containing p-nitrophenyl potassium sulfate crystals, and a third reagent well 433 for containing 3 ', 5' adenosine diphosphate powder, and the reagent well cover 45 comprises a first reagent well cover 451 sealably connected to the first reagent well 431, a second reagent well cover 452 sealably connected to the second reagent well 432, and a third reagent well cover 453 sealably connected to the third reagent well 433.
The reagent hole 43 and the reaction hole 44 are both circular, and the diameter of the reaction hole 44 is larger than that of the reagent hole 43, so that the detection reaction is facilitated. The three reagent wells 43 are the same size.
Wherein, the reagent hole cover 45 and the reaction hole cover 46 are respectively provided with a sealing ring to realize the sealing connection between the reagent hole cover 45 and the reagent hole 43 and the sealing connection between the reaction hole cover 46 and the reaction hole 44.
In this embodiment, the inner wall of the reaction hole 44 has no coating, and when the test reaction is performed, each of the reaction holes 44 is actually added as required.
Further, the reaction plate cover 42 can be connected to the reaction plate body 41 in a sealing manner, so as to further ensure the internal sealing performance of the reaction hole 44.
It should be understood that the above-described embodiments are merely illustrative of the preferred embodiments of the present invention and the technical principles thereof. It will be understood by those skilled in the art that various modifications, equivalents, changes, and the like can be made to the present invention. However, these modifications are within the scope of the present invention as long as they do not depart from the spirit of the present invention. In addition, certain terms used in the specification and claims of the present application are not limiting, but are used merely for convenience of description.
Claims (6)
1. The utility model provides a novel human scalp hair follicle endotheca sulfotransferase activity detect reagent box, includes the box body and is used for the selective shutoff lid of box body one side opening, its characterized in that still includes:
the lower layer container is placed in the box body and comprises a container body and a plurality of test tube grooves which are arranged on the container body at intervals, the openings of the test tube grooves are upward, a test tube is inserted into each test tube groove, and each test tube is used for containing a liquid reagent;
the upper reaction plate is placed in the box body and positioned above the lower container, the upper reaction plate comprises a reaction plate body and a reaction plate cover body, the reaction plate body selectively blocks the opening end of the reaction plate body, a plurality of reagent holes with upward openings and a reaction hole are formed in the reaction plate body at intervals, each reagent hole is used for containing a solid reagent, a reagent hole cover is arranged on the inner side of the reaction plate cover body corresponding to each reagent hole, a reaction hole cover is arranged corresponding to the reaction hole, when the reaction plate body is blocked by the reaction plate cover body, the reagent hole covers are in sealing connection with the corresponding reagent holes, and the reaction hole covers are in sealing connection with the reaction holes.
2. The novel human scalp intrafollicular sulfotransferase activity test kit of claim 1, wherein said test tubes comprise a first test tube for containing a phosphate buffer, a second test tube for containing a Tris-HCl buffer, and a third test tube for containing a magnesium chloride hexahydrate solution.
3. The novel human scalp intra-follicular sulfotransferase activity assay kit of claim 1, wherein said reagent wells comprise a first reagent well for containing minoxidil powder, a second reagent well for containing potassium p-nitrophenyl sulfate crystals and a third reagent well for containing adenosine 3 ', 5' diphosphate powder, said reagent well caps comprising a first reagent well cap sealingly connectable to said first reagent well, a second reagent well cap sealingly connectable to said second reagent well and a third reagent well cap sealingly connectable to said third reagent well.
4. The kit for detecting the activity of human scalp intrafollicular sulfotransferase according to claim 1, wherein said reagent well and said reaction well are both circular and have a diameter greater than the diameter of said reagent well.
5. The kit for detecting the activity of human scalp intrafollicular sulfotransferase according to claim 1, wherein said reagent well cover and said reaction well cover are provided with a sealing ring, respectively.
6. The kit for detecting the activity of human scalp endofollicular sulfotransferase of claim 1, wherein said reaction plate cover is sealingly attachable to said reaction plate body.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021428041.8U CN213139530U (en) | 2020-07-20 | 2020-07-20 | Novel human scalp hair follicle internal sulfotransferase activity detection kit |
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| CN202021428041.8U CN213139530U (en) | 2020-07-20 | 2020-07-20 | Novel human scalp hair follicle internal sulfotransferase activity detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4279608A1 (en) * | 2022-05-17 | 2023-11-22 | Follea International | Method for stabilizing colorimetric assay for use with plucked human hair |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4279608A1 (en) * | 2022-05-17 | 2023-11-22 | Follea International | Method for stabilizing colorimetric assay for use with plucked human hair |
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