CN213131095U

CN213131095U - Low-temperature cerebrospinal fluid circulating device for subarachnoid space of experimental animal

Info

Publication number: CN213131095U
Application number: CN202020794011.2U
Authority: CN
Inventors: 王力航; 汤倩
Original assignee: Guizhou Orthopedics Hospital
Current assignee: Guizhou Orthopedics Hospital
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2021-05-07
Anticipated expiration: 2030-05-14

Abstract

The utility model discloses a subarachnoid space low-temperature cerebrospinal fluid circulating device for experimental animals, which comprises a double-layer glass reaction kettle, wherein the surface of the double-layer glass reaction kettle is covered with a heat preservation layer, and the inner part of the double-layer glass reaction kettle is filled with nerve protection liquid, a capillary tube is wound on the outer surface of inner layer glass of the double-layer glass reaction kettle, two ends of the capillary tube are respectively connected with two ends of a pipeline, difluoroethane is arranged in the pipeline, the pipeline sequentially passes through a compressor and a condenser, a miniature waterproof thermistor is arranged in the nerve protection liquid, the output end of the miniature waterproof thermistor is connected with a controller of the compressor, the outlet end of the double-layer glass reaction kettle is connected with a peristaltic pump through a hose, the outlet end of the peristaltic pump is connected with a micro sample injection needle through a hose, the inlet end of the double-layer glass reaction kettle is connected with an injection needle through a hose, the injection, the output end of the temperature measuring line of the thermocouple is connected with a relay, and the relay is connected with a peristaltic pump.

Description

Low-temperature cerebrospinal fluid circulating device for subarachnoid space of experimental animal

Technical Field

The utility model relates to an experimental apparatus, in particular to arachnoid cavity low temperature cerebrospinal fluid circulating device for experimental animals.

Background

The nerve protection solution is required to be subjected to animal experiments before formal use, generally, the nerve protection solution is stored in a constant temperature environment, is injected into a cerebrospinal fluid pool of an subarachnoid space of an animal during the experiments, and is then extracted to see whether the nerve protection solution can play a role in cooling protection treatment on spinal cord tissues and whether the nerve protection solution can take away inflammatory factors of spinal cord injuries.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a low-temperature cerebrospinal fluid circulation device for subarachnoid space of experimental animals, which can automatically keep the temperature of the nerve protection fluid constant and automatically circulate the nerve protection fluid inside and outside the animal.

The utility model aims at realizing through the following technical scheme:

the utility model provides an experimental animals is with arachnoid cavity of resorption low temperature cerebrospinal fluid circulating device, includes double glass reation kettle, double glass reation kettle coats on the surface and is stamped the heat preservation, the splendid attire has nerve protection liquid in the double glass reation kettle, the winding has the capillary on double glass reation kettle's the inner glass surface, the capillary both ends link to each other with a pipeline both ends respectively, the aperture of capillary is greater than the aperture of pipeline, there is difluoroethane in the pipeline, the pipeline is in proper order through compressor and condenser, be equipped with miniature waterproof thermistor in the nerve protection liquid, miniature waterproof thermistor output links to each other with the controller of compressor, double glass reation kettle's exit end passes through the hose and links to each other with the peristaltic pump, the peristaltic pump exit end passes through the hose and links to each other with the micro-needle, double glass reation kettle entry end passes through the hose and links to each other, the injection needle and the sample injection needle are both inserted into a cerebrospinal fluid pool in a spinal canal of the experimental animal, a thermocouple tetrafluoro temperature measuring line extending into the cerebrospinal fluid pool is further arranged between the micro sample injection needle and the injection needle, the output end of the thermocouple tetrafluoro temperature measuring line is connected with a relay, and the relay is connected with a peristaltic pump.

Furthermore, the number of the thermocouple tetrafluoro temperature measuring lines is two, and the two thermocouple tetrafluoro temperature measuring lines respectively detect the animal temperatures at the sample injection needle and the injection needle.

The utility model has the advantages that:

the utility model discloses a low temperature cerebrospinal fluid circulating device of arachnoid cavity of resorption for experimental animals can make the nerve protection liquid circulate in the animal body and between double glass reation kettle through the nerve protection liquid in pipeline, compressor, condenser and the automatic constant temperature double glass reation kettle of capillary ability through hose, peristaltic pump, trace advance kind needle and syringe needle.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and/or combinations particularly pointed out in the appended claims.

Drawings

In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail with reference to the accompanying drawings, in which:

fig. 1 is a schematic structural diagram of the present invention.

Detailed Description

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the preferred embodiments are for purposes of illustration only and are not intended to limit the scope of the present invention.

As shown in fig. 1, a subarachnoid space low-temperature cerebrospinal fluid circulating device for experimental animals comprises a double-layer glass reaction kettle 1, wherein a heat-insulating layer covers the surface of the double-layer glass reaction kettle 1, a nerve protection solution is contained in the double-layer glass reaction kettle 1, the double-layer glass reaction kettle 1 can realize that the nerve protection solution is in an aseptic state, a capillary tube 2 is wound on the outer surface of inner-layer glass of the double-layer glass reaction kettle 1, two ends of the capillary tube 2 are respectively connected with two ends of a pipeline 3, the aperture of the capillary tube 2 is larger than that of the pipeline 3, difluoroethane is contained in the pipeline 3, the pipeline 3 sequentially passes through a compressor 4 and a condenser 5, a miniature waterproof thermistor 6 is arranged in the nerve protection solution, the output end of the miniature waterproof thermistor 6 is connected with a controller of the compressor 4, when the miniature waterproof thermistor 6 detects that the temperature of the nerve protection solution is higher than a set, the pressurized difluoroethane is condensed into a high-pressure low-temperature state by a condenser 5, the high-pressure low-temperature difluoroethane is changed into a low-temperature normal-pressure state in the capillary 2 because the aperture of the capillary 2 is larger than the aperture of a pipeline 3, the difluoroethane can absorb heat in the double-layer glass reaction kettle 1 to realize the temperature reduction of the nerve protection liquid, the outlet end of the double-layer glass reaction kettle 1 is connected with a peristaltic pump 7 through a hose, the outlet end of the peristaltic pump 7 is connected with a 100ul33g micro sample injection needle 8 through a hose, the inlet end of the double-layer glass reaction kettle 1 is connected with an injection needle 9 through a hose, the injection needle 9 and the micro sample injection needle 8 are both inserted into a cerebrospinal fluid pool in a vertebral canal of an experimental animal, a thermocouple tetrafluoro temperature measuring line 10 extending into the cerebrospinal fluid pool is also arranged between the micro sample injection needle 8 and the injection needle 9 (at the moment, the, the relay 11 is connected with the peristaltic pump 7, the nerve protection liquid is injected into a cerebrospinal fluid pool in a vertebral canal of an experimental animal through the peristaltic pump 7 and the micro sample injection needle 8 and then is pumped back into the double-layer glass reaction kettle 1 through the injection needle 9, when the temperature of the animal is detected by the polytetrafluoroethylene temperature measuring line 10 of the thermocouple to be higher than a set value, the peristaltic pump 7 is automatically started through the relay 11, and when the temperature of the animal is detected by the polytetrafluoroethylene temperature measuring line 10 of the thermocouple to be lower than the set value, the peristaltic pump 7 is closed through the relay 11.

The preferable thermocouple tetrafluoro temperature measuring lines 10 are two, the two thermocouple tetrafluoro temperature measuring lines 10 respectively detect the animal temperature at the micro-sampling needle 8 and the injection needle 9, and a relay starts a peristaltic pump when the temperature at any position is higher than a set value.

The condenser 5 in the utility model is provided by Shenzhen science power; the capillary tube 2 is provided by a Shanghai metal material; compressor 4 model CB32, supplied by mitsubishi heavy industry, japan; the double-layer glass reaction kettle 1 is provided by Zhengzhou Boke instruments; the relay 11 is provided by a Ningbo glow meter; thermocouple tetrafluoro thermometry line 10 is provided by U.S. ohm-m; the micro-needle 8 and 100ul33g syringe needle 8 are supplied by Hamilton, usa and the peristaltic pump 7 by baoding lange.

Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the scope of the claims of the present invention.

Claims

1. The utility model provides a subarachnoid space low temperature cerebrospinal fluid circulating device for experimental animals which characterized in that: including double glass reation kettle (1), double glass reation kettle (1) coats on the surface and is stamped the heat preservation, the splendid attire has nerve protection liquid in double glass reation kettle (1), the winding has capillary (2) on the inlayer glass surface of double glass reation kettle (1), capillary (2) both ends link to each other with a pipeline (3) both ends respectively, the aperture of capillary (2) is greater than the aperture of pipeline (3), there is difluoroethane in pipeline (3), pipeline (3) are in proper order through compressor (4) and condenser (5), be equipped with miniature waterproof thermistor (6) in the nerve protection liquid, miniature waterproof thermistor (6) output links to each other with the controller of compressor (4), the exit end of double glass reation kettle (1) passes through the hose and links to each other with peristaltic pump (7), peristaltic pump (7) exit end passes through the hose and links to each other with trace injection needle (8), the inlet end of the double-layer glass reaction kettle (1) is connected with an injection needle (9) through a hose, the injection needle (9) and a micro sample injection needle (8) are both inserted into a cerebrospinal fluid pool in an experimental animal vertebral canal, a thermocouple tetrafluoro temperature measuring line (10) extending into the cerebrospinal fluid pool is further arranged between the micro sample injection needle (8) and the injection needle (9), the output end of the thermocouple tetrafluoro temperature measuring line (10) is connected with a relay (11), and the relay (11) is connected with a peristaltic pump (7).

2. The subarachnoid space low-temperature cerebrospinal fluid circulating device for experimental animals as claimed in claim 1, wherein: the number of the thermocouple tetrafluoro temperature measuring lines (10) is two, and the two thermocouple tetrafluoro temperature measuring lines (10) respectively detect the animal temperature at the micro-sampling needle (8) and the injection needle (9).