CN213121613U - Portable fluorescence detector - Google Patents
Portable fluorescence detector Download PDFInfo
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- CN213121613U CN213121613U CN202021647965.7U CN202021647965U CN213121613U CN 213121613 U CN213121613 U CN 213121613U CN 202021647965 U CN202021647965 U CN 202021647965U CN 213121613 U CN213121613 U CN 213121613U
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Abstract
The utility model provides a portable fluorescence detector relates to fluorescence check out test set's technical field, has mainly solved prior art fluorescence detector and has aroused that the light path is long with the emission light path, logical light efficiency is not high, test speed is slow, sensitivity lower grade technical problem. The fluorescence detector comprises a micro spectrometer, a sample bin and a monochromatic light excitation unit, wherein the sample bin is used for placing a test sample. The sample bin comprises a first light path channel and a second light path channel, the first light path channel is perpendicular to the second light path channel, the first light path channel is connected with the monochromatic light excitation unit, and the second light path channel is connected with the micro spectrometer. The first light path channel of the sample chamber is vertical to the second light path channel, so that the second light path channel obtains the background with low stray light interference. The first light path channel is connected with the monochromatic light excitation unit, so that the excitation light path is shortened. The second light path channel is connected with the micro spectrometer, so that an emission light path is shortened, and the light transmission efficiency and the sensitivity of the device are improved.
Description
Technical Field
The utility model relates to a fluorescence detection equipment's technical field especially relates to a portable fluorescence detector.
Background
The fluorescence spectrum method is a high-sensitivity detection technology, the lowest detection limit can reach the fluorescein substance with femtomolar concentration, and the sensitivity is hundreds of times higher than that of the absorption spectrum. The existing spectrofluorometers (also called fluorescence spectrometers) for detecting fluorescence signals are composed of a 150W high-power xenon lamp light source, an excitation monochromator, a sample bin, an emission monochromator and a Photomultiplier Tube (PMT) single-point detector. The working principle of the xenon lamp continuous spectrum fluorescence spectrometer is that monochromatic light is generated by exciting a monochromator in a monochromatic mode, the monochromatic light enters a sample bin to irradiate a sample and generate a fluorescence signal of the continuous spectrum, the fluorescence signal enters an emission monochromator to be split and projected onto a PMT detector, and the fluorescence spectrum is obtained by rotating and scanning an emission monochromator grating.
The traditional fluorescence spectrometer has the advantages of complex structure, large volume, high energy consumption, low test speed and high price, can only be used for detection and analysis in medium and large laboratories, can not realize field or field detection, and is not beneficial to popularization and application of fluorescence technology.
SUMMERY OF THE UTILITY MODEL
The utility model discloses one of them purpose is in order to provide a portable fluorescence detector, solves prior art fluorescence detector and arouses that the light path is long with the emission light path, logical light efficiency is not high, test speed is slow and sensitivity is lower technical problem. The utility model discloses a great deal of beneficial effect can be reached in the preferred embodiment, specifically see the following explanation.
In order to achieve the above purpose, the utility model provides a following technical scheme:
the utility model discloses a portable fluorescence detector includes miniature spectrum appearance, sample storehouse and monochromatic light excitation unit, and the sample storehouse is used for placing test sample. The sample bin comprises a first light path channel and a second light path channel, the first light path channel is perpendicular to the second light path channel, the first light path channel is connected with the monochromatic light excitation unit, and the second light path channel is connected with the micro spectrometer.
The utility model relates to a further preferred technical scheme, the inner wall in sample storehouse is provided with black light-absorbing layer.
The utility model relates to a further preferred technical scheme, portable fluorescence detector still includes the filtering unit, and the filtering unit is located second light path passageway to filter the light that gets into in the second light path passageway through the filtering unit.
The utility model relates to a further preferred technical scheme, the light filtering unit is the long pass filter.
The utility model relates to a further preferred technical scheme, second light path channel's length is not more than 9 mm.
The utility model relates to a further preferred technical scheme, monochromatic light arouses the unit and includes the LED lamp.
In a further preferred embodiment of the present invention, the micro spectrometer is a full-spectrum direct-reading CCD (Charge-coupled Device) detector micro spectrometer.
The utility model relates to a further preferred technical scheme, portable fluorescence detector still includes control circuit, and control circuit excites unit and miniature spectrum appearance with monochromatic light and is connected.
As a preferred embodiment, the control circuit comprises a control switch to turn on or off the micro spectrometer and the monochromatic light excitation unit by the control switch.
The utility model relates to a further preferred technical scheme, portable fluorescence detector still includes the electric power storage unit, and the electric power storage unit passes through control circuit and is connected with monochromatic light excitation unit and miniature spectrum appearance.
As a further preferred embodiment, the electric storage unit includes a charging circuit.
As a further preferred embodiment, the charging circuit and the control circuit are integrated on a circuit board.
The utility model relates to a further preferred technical scheme, portable fluorescence detector still includes the casing, is provided with sample deposit reaction tank on the casing, and sample deposit reaction tank is used for the sample to store or is used for the sample reaction. And a positioning structure for fixing the micro spectrometer, the sample bin, the monochromatic light excitation unit, the control circuit and the electric power storage unit is arranged in the shell.
In a preferred embodiment, the positioning structure is arranged in the shell and used for supporting the micro spectrometer, the sample chamber, the monochromatic light excitation unit, the control circuit and the storage unit supporting frame or supporting column. Further preferably, the support micro spectrometer, the sample chamber, the monochromatic light excitation unit, the control circuit and the electric storage unit are fixed with the shell through screws.
The utility model provides a portable fluorescence detector has following beneficial technological effect at least:
fluorescence detector in sample storehouse first light path passageway and second light path passageway set up perpendicularly for second light path passageway obtains the background of low stray light interference. And, connect first light path channel and monochromatic light excitation unit and shortened the excitation light path, connect the emission light path that has shortened with miniature spectrum appearance with the second light path channel, and then ensure to improve the sensitivity of logical light efficiency and equipment.
Furthermore, the utility model discloses preferred technical scheme can also produce following technological effect:
the utility model discloses among the preferred technical scheme, the inner wall in sample storehouse sets up black light-absorbing layer, has further reduced stray light and has caused the interference to the transmission light path in the second light path passageway.
The utility model discloses among the preferred technical scheme, adopt the LED lamp as the light source of the short wavelength monochromatic light of narrowband, utilize miniature spectrum appearance to measure the transmission light path, can reduce fluorescence detector's energy consumption, reduce fluorescence detector's volume, and then the carrying of the fluorescence detector of being convenient for.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of a fluorescence detector according to a preferred embodiment of the present invention.
In the figure: 1-an electric storage unit; 2-monochromatic light excitation unit; 3-a sample bin; 31 — a first optical path channel; 32-a second optical path channel; 4-a filter unit; 5-a charging port; 6-a switch; 7-a control circuit; 8-micro spectrometer; 9-a sample storage reaction tank; 10-shell.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise specified, "a plurality" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood as the case may be, by those of ordinary skill in the art.
Referring to fig. 1, the fluorescence detector in one embodiment includes a micro spectrometer 8, a sample chamber 3, and a monochromatic light excitation unit 2, where the sample chamber 3 is used for placing a test sample. The sample chamber 3 comprises a first light path channel 31 and a second light path channel 32, the first light path channel 31 and the second light path channel 32 are perpendicular to each other, the first light path channel 31 is connected with the monochromatic light excitation unit 2, and the second light path channel 32 is connected with the micro spectrometer 8.
Preferably, the sample bin 3 is matched with a quartz fluorescence cuvette pool, and when the detection is carried out, the quartz fluorescence cuvette pool containing the sample is placed in the sample bin 3. Further preferably, be provided with in the sample storehouse 3 with the recess of quartz fluorescence cuvette pond looks adaptation, when detecting, the recess in the quartz fluorescence cuvette pond embedding sample storehouse 3 to pinpoint the quartz fluorescence cuvette pond. Preferably, the monochromatic light excitation unit 2 emits light as narrow-band short-wavelength monochromatic light.
The operation steps of the fluorescence detector described in the above preferred embodiment when testing a sample are as follows:
the sample to be measured is placed in the quartz fluorescence cuvette cell, and the quartz fluorescence cuvette cell is installed in the sample compartment 3 so that the quartz fluorescence cuvette cell is aligned with the first optical path channel 31 and the second optical path channel 32. The monochromatic light excitation unit 2 and the micro spectrometer 8 are then turned on. The narrow-band short-wavelength monochromatic light emitted by the monochromatic light excitation unit 2 passes through the first light path channel 31 and then irradiates on the liquid sample in the quartz fluorescence cuvette cell. A liquid sample in the quartz fluorescence cuvette pool emits a fluorescence signal after being irradiated by narrow-band short-wavelength light. The fluorescence emitted from the liquid sample in the quartz fluorescence cuvette cell enters the micro spectrometer 8 through the second optical path channel 32, and then the fluorescence spectrum is measured by the micro spectrometer 8.
It should be noted that, the traditional fluorescence spectrometer has a complex structure and a large volume, the longest side of the traditional fluorescence spectrometer is larger than 50cm, the weight of the traditional fluorescence spectrometer is not lower than 20kg, and the traditional fluorescence spectrometer is inconvenient to carry. Fluorescence detector's long limit size be less than 140mm, total mass is less than 1 kg. Compare with traditional fluorescence spectrum appearance, fluorescence detector portable.
It is further preferred that the length of the second optical path channel 32 is not more than 9 mm.
In the preferred embodiment, the emission light path adopts an ultra-short distance design, so that the collection efficiency of the fluorescence signal is improved, and the analysis sensitivity of the micro spectrometer 8 can be further improved.
As a preferred embodiment of the present invention, the inner wall of the sample chamber 3 is provided with a black light absorbing layer. As a preferred embodiment, the inner wall of the sample chamber 3 is processed by a matte process to form a black light absorbing layer on the inner wall of the sample chamber 3, and further to form a light trap on the inner wall of the sample chamber 3, so that the micro spectrometer 8 can obtain a background with low stray light interference.
The utility model relates to a fluorescence detector in further embodiment still includes filtering unit 4, and filtering unit 4 is located second light path 32 to filter the light that gets into in second light path 32 through filtering unit 4. Preferably, the filter unit 4 is a long-pass filter.
The long-pass filter is used to transmit light longer than the cutoff wavelength and to shield light shorter than the cutoff wavelength by absorption or reflection. In the above preferred embodiment, the rayleigh signal can be completely shielded and the frequency doubling signal can be eliminated by arranging the filtering unit 4, so that the micro spectrometer 8 obtains ultra-low stray light, and the detection sensitivity is improved.
In a further embodiment, the monochromatic light excitation unit 2 comprises a LED lamp. Preferably, the LED lamp is a light emitting diode.
In the preferred embodiment, the LED lamp as the monochromatic light excitation unit 2 consumes less power than the conventional 150W xenon lamp light source. In a preferred embodiment of the present invention, the power storage unit 1 is a high-energy-density lithium battery, which can maintain the continuous irradiation time of the LED light source longer than 100 hours, and can be continuously used for at least 15 days, calculated according to 7 hours of operation per day.
As a further preferred embodiment of the present invention, the micro spectrometer 8 is a full-spectrum direct-reading CCD detector micro spectrometer.
It should be noted that, the full-spectrum direct-reading CCD detector micro spectrometer can obtain full spectrum at one time without grating rotation, the shortest acquisition time is 0.01 second, and the detection speed is ultra-fast. The micro spectrometer of the full-spectrum direct-reading CCD detector is selected in the embodiment, so that the detection efficiency can be effectively improved.
The utility model relates to a in the further embodiment, fluorescence detector still includes control circuit 7, and control circuit 7 is connected with monochromatic light excitation unit 2 and miniature spectrum appearance 8.
The utility model relates to a fluorescence detector among further preferred embodiment still includes electric power storage unit 1, and electric power storage unit 1 passes through control circuit 7 and is connected with monochromatic light excitation unit 2 and miniature spectrum appearance 8.
Referring to fig. 1, it is further preferred that the control circuit 7 further comprises a switch 6 to turn on or off the monochromatic light excitation unit 2 and/or the micro spectrometer 8 through the switch 6. Preferably, the switch 6 is a push button switch.
Further preferably, the power storage unit 1 includes a charging circuit.
As a further preferred embodiment, the charging circuit and the control circuit 7 are integrated on a circuit board.
Referring to fig. 1, the fluorescence detector according to a further preferred embodiment of the present invention further includes a housing 10, wherein the housing 10 is provided with a sample storage reaction tank 9, and the sample storage reaction tank 9 is used for storing a sample or reacting the sample. And a positioning structure for fixing the micro spectrometer 8, the sample bin 3, the monochromatic light excitation unit 2, the control circuit 7 and the electric storage unit 1 is arranged in the shell 10.
Referring to fig. 1, preferably, the fluorescence detector includes a plurality of the sample storage reaction chambers 9.
As a further preferred embodiment, the supporting micro spectrometer 8, the sample chamber 3, the monochromatic light excitation unit 2, the control circuit 7 and the electric storage unit 1 are fixed to the housing 10 by screws.
Further preferably, the charging circuit includes a charging port 5. Preferably, the charging port 5 and the switch 6 are fixedly provided on the housing 10.
It should be noted that, in an embodiment of the present invention, the fluorescence detector utilizes a high-energy-density lithium battery to supply power to a low-energy-consumption light emitting diode, i.e., a LED lamp, so that the device can operate for a very long time without an external power source, and can be widely applied to the field or remote areas. In a preferred embodiment, the fluorescence detector improves the excitation light path, i.e. the first light path channel 31, and the emission light path, i.e. the second light path channel 32, so that the excitation light path and the emission light path are shortened, and the passing efficiency of the excitation light path and the emission light path and the sensitivity of the device are improved.
The utility model relates to an in the embodiment fluorescence detector reduce instrument volume and instrument cost, reduce energy consumption and weight for equipment can apply to the fluorescence analysis detection application that most excitation wavelength was confirmed, for example the content detection of one or several kinds of material.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A portable fluorescence detector is characterized by comprising a micro spectrometer (8), a sample bin (3) and a monochromatic light excitation unit (2), wherein the sample bin (3) is used for placing a test sample; sample storehouse (3) include first light path passageway (31) and second light path passageway (32), first light path passageway (31) with second light path passageway (32) mutually perpendicular, just first light path passageway (31) with monochromatic light arouses unit (2) and connects, second light path passageway (32) with miniature spectrum appearance (8) are connected.
2. The portable fluorescence detector of claim 1, wherein the inner wall of the sample chamber (3) is provided with a black light absorbing layer.
3. The portable fluorescence detector of claim 1 or 2, further comprising a filter unit (4), the filter unit (4) being located on the second light path channel (32) to filter excitation light rays entering the second light path channel (32) through the filter unit (4).
4. The portable fluorescence detector of claim 3, wherein the filter unit (4) is a long pass filter.
5. The portable fluorescence detector of claim 4, wherein the length of the second optical path (32) is no greater than 9 mm.
6. The portable fluorescence detector of claim 1, wherein the monochromatic light excitation unit (2) comprises an LED lamp.
7. The portable fluorescence detector of claim 1, wherein the micro spectrometer (8) is a full spectrum direct read CCD detector micro spectrometer.
8. The portable fluorescence detector of claim 1, further comprising a control circuit (7), the control circuit (7) being connected to the monochromatic light excitation unit (2) and the micro spectrometer (8).
9. The portable fluorescence detector according to claim 8, further comprising an electrical storage unit (1), wherein the electrical storage unit (1) is connected to the monochromatic light excitation unit (2) and the micro spectrometer (8) through the control circuit (7).
10. The portable fluorescence detector of claim 9, further comprising a housing (10), wherein the housing (10) is provided with a sample storage reaction tank (9), and the sample storage reaction tank (9) is used for storing a sample or reacting the sample; and a positioning structure for fixing the micro spectrometer (8), the sample bin (3), the monochromatic light excitation unit (2), the control circuit (7) and the electric power storage unit (1) is arranged in the shell (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021647965.7U CN213121613U (en) | 2020-08-10 | 2020-08-10 | Portable fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021647965.7U CN213121613U (en) | 2020-08-10 | 2020-08-10 | Portable fluorescence detector |
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| Publication Number | Publication Date |
|---|---|
| CN213121613U true CN213121613U (en) | 2021-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021647965.7U Active CN213121613U (en) | 2020-08-10 | 2020-08-10 | Portable fluorescence detector |
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| CN (1) | CN213121613U (en) |
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- 2020-08-10 CN CN202021647965.7U patent/CN213121613U/en active Active
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