CN213113339U - Cell state monitoring facilities - Google Patents
Cell state monitoring facilities Download PDFInfo
- Publication number
- CN213113339U CN213113339U CN202020529730.1U CN202020529730U CN213113339U CN 213113339 U CN213113339 U CN 213113339U CN 202020529730 U CN202020529730 U CN 202020529730U CN 213113339 U CN213113339 U CN 213113339U
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- China
- Prior art keywords
- cell culture
- culture dish
- cell
- scanning electron
- electron microscope
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- 238000012544 monitoring process Methods 0.000 title claims description 22
- 238000004113 cell culture Methods 0.000 claims abstract description 80
- 238000002347 injection Methods 0.000 claims abstract description 23
- 239000007924 injection Substances 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 230000001580 bacterial effect Effects 0.000 claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 5
- 239000000645 desinfectant Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000005485 electric heating Methods 0.000 abstract description 10
- 238000012806 monitoring device Methods 0.000 abstract description 4
- 208000035143 Bacterial infection Diseases 0.000 abstract 1
- 208000022362 bacterial infectious disease Diseases 0.000 abstract 1
- 241000233866 Fungi Species 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 6
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000012930 cell culture fluid Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell state monitoring device, which comprises a scanning electron microscope, an electric heating thermostat, a bacterial filter and an injection tube, wherein a cell culture bottle is arranged in the electric heating thermostat, a cell culture dish is fixedly arranged on a placing table of the scanning electron microscope through a clamping mechanism, a first through hole communicated with the inside of the cell culture dish is arranged on one side of the lower surface of the cell culture dish, which is far away from the scanning electron microscope, one end of a first catheter is connected in the first through hole, one end of a second catheter is extended into the inside of the cell culture bottle, the other end of the second catheter sequentially penetrates through the cover body of the cell culture bottle and the side wall of the electric heating thermostat and is fixedly connected with the other end of the first catheter, the utility model can move a push-pull rod at intervals to guide cell culture liquid into the cell culture dish, the state of the cells is monitored, and the cells are prevented from being directly poured into a cell culture dish to cause bacterial infection of the cells.
Description
Technical Field
The utility model relates to a cell monitoring technology field, concrete field is a cell state monitoring facilities.
Background
The cells are generally good in shape and clear in outline under the sterile state, in addition, the proliferation condition is good, so that the cells can always keep a normal state, if the cells are infected by bacteria, the cells can have different shapes according to different infected bacteria, the culture solution generally becomes turbid and yellow, the influence on the cell growth is obvious, if the cells are infected by mould fungi, the culture solution is clear, the impurities do not exist under an inverted microscope, but flocculent impurities exist, filamentous floating objects can be seen under the microscope, obvious hyphae can be seen, the cells can still grow, after the time is long, the vitality state of the cells is poor, if the cells are infected by the fungi, the culture solution is clear and does not change color, filaments exist under the microscope, some fungi start to be like dead cell fragments, but a plurality of small blocks are clear, the coral-like shapes do not like the broken cell fragments are not clear, the fine black filaments can grow slowly, the growth of the fungi is slow, unlike bacteria, it is easy to detect, but once its presence is detected, the cells become contaminated and are difficult to survive.
The existing cell state monitoring equipment usually needs to pour cell culture solution into a vessel, then observe through a microscope, pour repeatedly, easily cause the infection of fungus, make cell culture failure.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell state monitoring facilities to it often needs to pour cell culture liquid into household utensils to propose current cell state monitoring facilities in solving above-mentioned background art, then observes through the microscope, pours into repeatedly, causes the infection of fungus class very easily, makes the problem of cell culture failure.
In order to achieve the above object, the utility model provides a following technical scheme: a cell state monitoring device comprises a scanning electron microscope, an electric heating thermostat, a bacteria filter and an injection tube, wherein a cell culture bottle is arranged inside the electric heating thermostat, a cell culture dish is fixedly arranged on a placing table of the scanning electron microscope through a clamping mechanism, a first through hole communicated with the inside of the cell culture dish is arranged on one side, away from the scanning electron microscope, of the lower surface of the cell culture dish, one end of a first liquid guide tube is connected in the first through hole, one end of a second liquid guide tube is stretched into the inside of the cell culture bottle, the other end of the second liquid guide tube sequentially penetrates through the cover body of the cell culture bottle and the side wall of the electric heating thermostat and is fixedly connected with the other end of the first liquid guide tube, a second through hole communicated with the inside of the cell culture dish is arranged on one side, away from the scanning electron microscope, of the upper surface of the, one end port of the bacterial filter is connected in the second through hole, the other end port of the bacterial filter is connected with an injection port of an injection cylinder, a piston is connected in the injection cylinder in a sliding mode, one side, far away from the bacterial filter, of the piston is fixedly provided with one end of a push-pull rod, and the other end of the push-pull rod penetrates through the top surface of the injection cylinder.
Preferably, the clamping mechanism comprises a clamping rod, one end of the clamping rod is fixedly installed on the lower surface of the cell culture dish and close to the position of the first liquid guide pipe, a threaded cylinder is fixedly installed at the other end of the clamping rod, a threaded knob is connected with the thread of the threaded cylinder in a threaded manner, and the threaded knob abuts against the lower surface of the placing table of the scanning electron microscope.
Preferably, the interior of the syringe barrel and the upper portion of the piston are filled with a disinfectant.
Preferably, the side wall of the piston is provided with a ring groove, and a sealing rubber sleeve is sleeved in the ring groove.
Preferably, a vent hole penetrates through the cover body of the cell culture bottle, and a vent pipe is arranged in the vent hole.
Preferably, the first catheter is a hose.
Preferably, a rotating handle is fixedly arranged at one end of the push-pull rod positioned outside the injection cylinder.
Compared with the prior art, the beneficial effects of the utility model are that: a cell state monitoring device, through the electric thermostat, can make the cell culture fluid keep the invariable temperature, can sterilize the inside of the electric thermostat before putting into, so as to make the cell preserve and develop in the suitable environment, through the cooperation setting of the syringe, piston and push-pull rod, when pulling the push-pull rod, the cell culture fluid in the cell culture bottle will enter into first catheter and second catheter sequentially, then enter into the cell culture dish, through the scanning electron microscope, can observe whether the cell state in the cell culture dish is normal, after monitoring, promote the push-pull rod, make the cell culture fluid return to the cell culture bottle, continue culturing its cell, through the cooperation setting of holding rod, threaded cylinder and threaded knob, can fix the cell culture dish on the placing table of the scanning electron microscope, and can carry on the regulation of the position, in order to reach better monitoring position, can prevent through bacterial filter when the activity push-pull rod, the fungus class material enters into the cell culture dish and causes the pollution to the cell, the utility model discloses can move about the push-pull rod at interval a period, with the leading-in cell culture liquid to the cell culture dish in, carry out state monitoring to the cell, prevent directly to pour into the cell culture dish in, cause the fungus class infection of cell.
Drawings
FIG. 1 is a schematic sectional view of the present invention;
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is an enlarged view of the structure at B in FIG. 1;
fig. 4 is a schematic view of the structure of the present invention.
In the figure: 1-scanning electron microscope, 2-electric thermostat, 3-cell culture bottle, 4-cell culture dish, 5-first liquid guide tube, 6-second liquid guide tube, 7-bacterial filter, 8-injection tube, 9-piston, 10-push-pull rod, 11-clamping rod, 12-threaded cylinder, 13-threaded knob, 14-disinfectant, 15-sealing rubber sleeve and 16-vent pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a cell state monitoring device comprises a scanning electron microscope 1, an electric heating incubator 2, a bacteria filter 7 and an injection tube 8, wherein a cell culture bottle 3 is arranged inside the electric heating incubator 2, the electric heating incubator 2 is selected from H2451, and the H2451 is suitable for being used as a culture experiment in the aspects of bacteria culture, breeding, fermentation and the like in scientific research departments such as industrial and mining enterprises, medical sanitation, biochemistry, agricultural science and the like. The temperature control instrument microcomputer intelligent instrument has the advantages of high temperature control precision, good stability, upper limit alarm, timing automatic power off and the like, a cell culture dish 4 is fixedly arranged on a placing table of a scanning electron microscope 1 through a clamping mechanism, the cell culture dish 4 adopts a thin ware piece with high transparency, whether the cell state in the cell culture dish 4 is normal can be observed through the scanning electron microscope 1, one side of the lower surface of the cell culture dish 4, which is far away from the scanning electron microscope 1, is provided with a first through hole communicated with the inside of the cell culture dish 4, one end of a first liquid guide pipe 5 is connected in the first through hole, one end of a second liquid guide pipe 6 is inserted in the cell culture bottle 3, the other end of the second liquid guide pipe 6 sequentially penetrates through the cover body of the cell culture bottle 3 and the side wall of an electric heating constant temperature box 2 and is fixedly connected with the other end of, a second through hole communicated with the interior of the cell culture dish 4 is formed in one side, away from the scanning electron microscope 1, of the upper surface of the cell culture dish 4, a port of a bacterial filter 7 is connected in the second through hole, the other port of the bacterial filter 7 is connected with an injection port of an injection tube 8, the bacterial filter 7 can prevent fungus substances from entering the cell culture dish 4 to pollute cells when a push-pull rod 10 is moved, a piston 9 is connected in the injection tube 8 in a sliding manner, one side, away from the bacterial filter 7, of the piston 9 is fixedly provided with one end of the push-pull rod 10, the other end of the push-pull rod 10 penetrates through the top surface of the injection tube 8, and through the matching arrangement of the injection tube 8, the piston 9 and the push-pull rod 10, when the push-pull rod 10 is pulled, cell culture liquid in the cell culture bottle 3 sequentially enters the first liquid guide tube 5 and the second liquid guide, after the monitoring is finished, the cell culture solution is returned to the cell culture flask 3 by pushing the push-pull rod 10 through the scanning electron microscope 1, and the cells are continuously cultured.
Particularly, fixture includes supporting rod 11, supporting rod 11 is the L type form, the one end fixed mounting of supporting rod 11 is at the lower surface of cell culture dish 4 and the position that is close to first catheter 5, the other end fixed mounting of supporting rod 11 has screw thread section of thick bamboo 12, screw thread section of thick bamboo 12 female connection has screw thread knob 13, screw thread knob 13 pushes up and leans on placing a lower surface position at scanning electron microscope 1, through supporting rod 11, screw thread section of thick bamboo 12 and screw thread knob 13's cooperation sets up, can fix cell culture dish 4 on scanning electron microscope 1 places the platform, and can carry out the regulation of position, in order to reach better monitoring position.
Specifically, the disinfectant 14 is filled in the syringe 8 and above the piston 9, and the syringe 8 can be sterilized by the disinfectant 14, thereby preventing contamination of the cells in the cell culture dish 4.
Specifically, the side wall of the piston 9 is provided with an annular groove, a sealing rubber sleeve 15 is sleeved in the annular groove, and the disinfectant 14 is prevented from entering the bacterial filter 7 when the piston 9 moves.
Specifically, a vent hole is formed through the lid of the cell culture bottle 3, and a vent tube 16 is provided in the vent hole, so that the piston 9 can be pulled more easily.
Specifically, the first catheter 5 is a flexible tube, which can be bent to facilitate the movement of the cell culture dish 4, so that the cell culture dish 4 can be placed at a proper position.
Specifically, a rotating handle is fixedly installed at one end of the push-pull rod 10 located outside the syringe 8, so that the push-pull rod 10 can be pushed or pulled conveniently.
The working principle is as follows: the utility model discloses in can pack into cell culture solution wherein and cultivate the cell through cell culture bottle 3, in the culture process, put into electric thermotank 2 with cell culture bottle 3, in order to keep invariable temperature, can carry out the disinfection and sterilization to electric thermotank 2 inside before putting into, so that the cell is preserved and is launched in suitable environment, through injection tube 8, piston 9 and push-and-pull rod 10's cooperation setting, when pulling push-and-pull rod 10, the cell culture solution in cell culture bottle 3 will enter into first catheter 5 and second catheter 6 in proper order, then enter into cell culture dish 4, cell culture dish 4 adopts the ware spare that is thin and the transparency is high, through scanning electron microscope 1, whether can observe the cell state in the cell culture dish 4 normal, after the monitoring, push-and-pull rod 10 is being promoted, make cell culture solution return to cell culture bottle 3 in, continuing to cultivate its cell, through the cooperation setting of supporting rod 11, screw thread section of thick bamboo 12 and screw knob 13, can fix cell culture dish 4 on scanning electron microscope 1 places the bench, and can carry out the regulation of position, in order to reach better monitoring position, can prevent through bacterial filter 7 when activity push-and-pull rod 10, fungus class material enters into cell culture dish 4 and causes the pollution to the cell, through disinfectant 14, can disinfect injection tube 8, also prevent the cell pollution in the cell culture dish 4, the utility model discloses can move about push-and-pull rod 10 at an interval, lead into cell culture liquid in the cell culture dish 4, carry out state monitoring to the cell, prevent directly pouring into in cell culture dish 4, cause the fungus class infection of cell.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a cell state monitoring facilities, includes scanning electron microscope (1), electric heat thermostated container (2), bacterial filter (7) and injection cylinder (8), its characterized in that: the inside of electric heat thermostated container (2) is equipped with cell culture bottle (3), scanning electron microscope (1) place the bench through fixture fixed mounting have cell culture dish (4), one side that scanning electron microscope (1) was kept away from to the lower surface of cell culture dish (4) is equipped with the first through-hole with the inside intercommunication of cell culture dish (4), the one end of first catheter (5) is connected to the in-hole in-line, the inside of cell culture bottle (3) stretches into the one end that has second catheter (6), the other end of second catheter (6) run through the lid of cell culture bottle (3) and the lateral wall of electric heat thermostated container (2) in proper order and with the other end fixed connection of first catheter (5), one side that scanning electron microscope (1) was kept away from to the upper surface of cell culture dish (4) is equipped with the second perforating hole with the inside intercommunication of cell culture dish (4), one end port of the bacterial filter (7) is connected in the second through hole, the other end port of the bacterial filter (7) is connected with an injection port of an injection cylinder (8), a piston (9) is connected in the injection cylinder (8) in a sliding mode, one side, far away from the bacterial filter (7), of the piston (9) is fixedly provided with one end of a push-pull rod (10), and the other end of the push-pull rod (10) penetrates through the top surface of the injection cylinder (8).
2. A cell state monitoring apparatus according to claim 1, wherein: the clamping mechanism comprises a clamping rod (11), one end of the clamping rod (11) is fixedly installed on the lower surface of a cell culture dish (4) and is close to the position of a first liquid guide pipe (5), a threaded cylinder (12) is fixedly installed at the other end of the clamping rod (11), a threaded knob (13) is connected with the inner thread of the threaded cylinder (12), and the threaded knob (13) abuts against the lower surface of a placing table of the scanning electron microscope (1).
3. A cell state monitoring apparatus according to claim 1, wherein: the interior of the injection cylinder (8) and the upper part of the piston (9) are filled with disinfectant (14).
4. A cell state monitoring apparatus according to claim 1, wherein: the side wall of the piston (9) is provided with a ring groove, and a sealing rubber sleeve (15) is sleeved in the ring groove.
5. A cell state monitoring apparatus according to claim 1, wherein: the cover body of the cell culture bottle (3) is provided with a vent hole in a penetrating way, and a vent pipe (16) is arranged in the vent hole.
6. A cell state monitoring apparatus according to claim 1, wherein: the first liquid guide pipe (5) is a flexible pipe.
7. A cell state monitoring apparatus according to claim 1, wherein: and a rotating handle is fixedly arranged at one end of the push-pull rod (10) positioned outside the injection cylinder (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020529730.1U CN213113339U (en) | 2020-04-13 | 2020-04-13 | Cell state monitoring facilities |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020529730.1U CN213113339U (en) | 2020-04-13 | 2020-04-13 | Cell state monitoring facilities |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213113339U true CN213113339U (en) | 2021-05-04 |
Family
ID=75643581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020529730.1U Expired - Fee Related CN213113339U (en) | 2020-04-13 | 2020-04-13 | Cell state monitoring facilities |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213113339U (en) |
-
2020
- 2020-04-13 CN CN202020529730.1U patent/CN213113339U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210504 |