CN213076783U

CN213076783U - Drainage device for enrichment and primary culture of tumor exfoliated cells

Info

Publication number: CN213076783U
Application number: CN202020735339.7U
Authority: CN
Inventors: 李梅; 胡梦文; 杨周
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-07
Filing date: 2020-05-07
Publication date: 2021-04-30
Anticipated expiration: 2030-05-07

Abstract

The utility model discloses a tumour cell enrichment and primary culture drainage ware that drops, including household utensils, platform and mouth of pipe connection structure, the fixed tray that is provided with in the upper left side of household utensils inside, and the top of tray settles and have the cell culture dish, the platform sets up in the top of cell culture dish, and the top of platform is provided with the connecting pipe, mouth of pipe connection structure sets up in the upper end of connecting pipe, and mouth of pipe connection structure's upper end is connected with ordinary drainage tube, the up end of household utensils is provided with the apron, and the left side of apron is connected with the hinge to the up end of apron is fixed with the handle. The whole body of the drainage device for the enrichment of the tumor exfoliated cells and the primary culture is made of transparent polyurethane heat-insulating materials, so that the drainage device not only can play a role in insulating body fluid which is just drained from a patient, but also is beneficial to the survival of the exfoliated cells, and simultaneously, a clinician can conveniently observe the condition of the drainage fluid in real time; greatly improves the practicability of the drainage device for the enrichment of the tumor exfoliated cells and the primary culture.

Description

Drainage device for enrichment and primary culture of tumor exfoliated cells

Technical Field

The utility model relates to a cell enrichment and culture apparatus technical field specifically are tumour cast-off cell enrichment and primary culture drainage ware.

Background

Many patients with malignant tumors of the pleuroperitoneal cavity adopt a way of draining pleuroperitoneal cavity and ascites to reduce the pressure of the pleuroperitoneal cavity, and meanwhile, the cast-off cells are extracted from the pleuroperitoneal cavity and the ascites to carry out related pathological detection. Clinically, sufficient living cancer cells are required to be subjected to activity detection or laboratory primary culture, and even if a large amount of pleural effusion and ascites are collected, a large amount of exfoliated cells die because the cells cannot be collected in time, or liquid in a drainage bottle is old/seriously polluted/the temperature in the drainage bottle is too low, or the cells are centrifuged before the exfoliated cells are extracted; for the difficulty caused by relevant detection and basic research, a drainage device for the enrichment and primary culture of the exfoliated tumor cells with higher practicability is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide tumour cell enrichment and primary culture drainage ware that drops to the collection that proposes in solving above-mentioned background art has often because can not in time collect, or liquid old state/pollution serious/temperature is too low in the drainage bottle, or in the off-center before drawing the cell that drops, the dead problem of a large amount of cells that drop.

In order to achieve the above object, the utility model provides a following technical scheme: tumour cell enrichment and primary culture drainage ware that drops, including household utensils, platform and mouth of pipe connection structure, the fixed tray that is provided with in the upper left side of household utensils inside, and the top of tray settles and have the cell culture dish, the platform sets up in the top of cell culture dish, and the top of platform is provided with the connecting pipe, mouth of pipe connection structure sets up in the upper end of connecting pipe, and the upper end of mouth of pipe connection structure is connected with ordinary drainage tube, the up end of household utensils is provided with the apron, and the left side of apron is connected with the hinge to the up end of apron is fixed with the handle.

Preferably, the right side of the vessel is connected with a connecting pipeline, and the right side of the connecting pipeline is connected with a negative pressure device.

Preferably, the utensil is communicated with the negative pressure device through a connecting pipeline, and the cover plate and the utensil form a rotating structure through a hinge.

Preferably, the side surface of the cell culture dish is provided with a side hole, and the distance between the side hole and the bottom end of the cell culture dish is 3-4 mm.

Preferably, the middle of the connecting pipe is provided with a transfer ball, a cell filter screen is arranged in the transfer ball, and the diameter of the cell filter screen is 40 um.

Preferably, the tube orifice connecting structure comprises a first connecting thread, a connecting raised head and a second connecting thread, the first connecting thread is arranged inside the tail end of the common drainage tube, the connecting raised head is arranged at the top end of the connecting tube, the second connecting thread is arranged on the outer surface of the connecting raised head, and the connecting tube is in threaded connection with the common drainage tube through the tube orifice connecting structure.

Compared with the prior art, the beneficial effects of the utility model are that:

the whole body of the drainage device for the enrichment of the tumor exfoliated cells and the primary culture is made of transparent polyurethane heat-insulating materials, so that the drainage device not only can play a role in insulating body fluid which is just drained from a patient, but also is beneficial to the survival of the exfoliated cells, and simultaneously, a clinician can conveniently observe the condition of the drainage fluid in real time;

the cover plate can be opened through rotation, so that the vessel can be lifted, the inner part of the vessel can be ensured to be in a relatively closed space through covering, and the inner part of the vessel can be kept in a negative pressure state through the arranged negative pressure device;

the arranged platform can reduce the phenomenon that body fluid directly flows to the cell culture dish and water flow impacts to disturb cell adherence, the side hole penetrates through the cell culture dish, and when the body fluid volume exceeds the side hole, redundant fluid in the cell culture dish flows downwards along the side so as to help the cell to adhere to the wall and survive as soon as possible; the cell filter screen in the transfer ball can filter most of impurities, and the preliminary screening cell makes its low reaches that flow into the drainage ware, also can suitably slow down the velocity of flow of drainage liquid simultaneously, has improved the practicality of this kind of tumour exfoliative cell enrichment and primary culture drainage ware greatly.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the upper end face structure of the vessel of the present invention;

fig. 3 is a schematic view of the pipe orifice connecting structure of the present invention.

In the figure: 1. a vessel; 2. a tray; 3. a cell culture dish; 4. a side hole; 5. a platform; 6. a connecting pipe; 7. a transfer ball; 8. a cell strainer; 9. a common drainage tube; 10. a cover plate; 11. a handle; 12. connecting a pipeline; 13. a negative pressure device; 14. a pipe orifice connecting structure; 141. a first connecting thread; 142. connecting the raised head; 143. a second connecting thread; 15. and (7) a hinge.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a drainage device for enrichment and primary culture of tumor exfoliated cells comprises a vessel 1, a platform 5 and a tube opening connecting structure 14, wherein a tray 2 is fixedly arranged at the upper left side inside the vessel 1, a cell culture dish 3 is arranged above the tray 2, the platform 5 is arranged above the cell culture dish 3, a connecting tube 6 is arranged above the platform 5, a side hole 4 is formed in the side surface of the cell culture dish 3, the distance between the side hole 4 and the bottom end of the cell culture dish 3 is 3-4mm, the arranged platform 5 can reduce body fluid from directly flowing to the cell culture dish, water flow impact interferes with cell adherence, the side hole 4 penetrates through the cell culture dish 3, when the body fluid volume exceeds the side hole, redundant fluid in the cell culture dish 3 adheres to the wall and downwards to help the cells to survive downstream, a transfer ball 7 is arranged in the middle of the connecting tube 6, and a cell filter screen 8 is arranged inside the transfer ball 7, and the mesh diameter of the cell filter screen 8 is 40um, the cell filter screen 8 in the transfer ball 7 can filter most impurities, primarily screen cells to enable the cells to flow into the downstream of the drainage device, and simultaneously can properly slow down the flow velocity of drainage liquid,

the pipe orifice connecting structure 14 is arranged at the upper end of the connecting pipe 6, the upper end of the pipe orifice connecting structure 14 is connected with a common drainage pipe 9, the upper end surface of the vessel 1 is provided with a cover plate 10, the left side of the cover plate 10 is connected with a hinge 15, the upper end surface of the cover plate 10 is fixed with a handle 11, the right side of the vessel 1 is connected with a connecting pipeline 12, the right side of the connecting pipeline 12 is connected with a negative pressure device 13, the vessel 1 is communicated with the negative pressure device 13 through the connecting pipeline 12, the cover plate 10 forms a rotating structure with the vessel 1 through the hinge 15, the pipe orifice connecting structure 14 comprises a first connecting thread 141, a connecting raised head 142 and a second connecting thread 143, the interior of the tail end of the common drainage pipe 9 is provided with the first connecting thread 141, the top end of the connecting pipe 6 is provided with the connecting raised head 142, the outer surface of the connecting raised head 142 is provided with the second connecting thread, the cover plate 10 can be opened by rotating, so that the vessel 1 can be lifted, the inner part of the vessel 1 can be ensured to be in a relatively closed space by closing, and the negative pressure device 13 can be arranged to keep the inner part of the vessel 1 in a negative pressure state.

The working principle is as follows: for the drainage device, firstly, the common drainage tube 9 is communicated with the abdominal cavity of a human body, so that the ascites in the abdominal cavity can be led out, the connecting tube 6 can be mutually connected with the first connecting thread 141 in the bottom end of the common drainage tube 9 through the connecting raised head 142 and the first connecting thread 141, so that the connection stability can be ensured, an expanded transfer ball 7 with the diameter of 2cm is arranged at the position of about 5cm of the interface of the connecting tube 6, a cell filter screen 8 with the aperture of 40um is arranged in the transfer ball 7, so that most impurities can be filtered in the transfer ball 7, cells are primarily screened to flow into the downstream of the drainage device, meanwhile, the flow rate of drainage liquid can be properly slowed down, the body liquid flows into a downstream vessel 1 after passing through the connecting tube 6 with the diameter of about 50cm, the body liquid firstly passes through the platform 5 in the vessel 1 and then flows down along the platform 5 along the wall to flow to the cell, collagen is coated at the bottom of the cell culture dish 3 to facilitate the cells to survive in a fast adherent manner, a side hole 4 is formed at a position 3-4mm higher than the bottom of the cell culture dish 3, and when the amount of body fluid exceeds the side hole 4, the redundant fluid in the culture dish flows down and flows into the vessel 1; the household utensils are guaranteed to be static as far as possible, when a patient gets off the bed to move, the common drainage bag is replaced at the accessible connector, the connector end of the drainage device is plugged by the sterile pipe plug, the wall is attached to the wall at 12 hours or 24 hours, the cover plate 10 is stirred by a doctor or an experimenter through the handle 11, the cover plate 10 can be rotated through the hinge 15 to open the household utensils 1, meanwhile, a cushion is inlaid at the joint of the cover plate 10 and the household utensils 1, so that when the cover plate 10 is closed, the sealing performance inside the household utensils 1 is guaranteed, the cell culture dishes 3 are taken out, meanwhile, the negative pressure device 13 can be communicated with the household utensils 1 through the connecting pipeline 12, the household utensils 1 are guaranteed to have proper negative pressure, and the use process of the.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Tumour cell enrichment and primary culture drainage ware that drops includes household utensils (1), platform (5) and mouth of pipe connection structure (14), its characterized in that: the utility model discloses a cell culture dish, including household utensils (1), top left side of inside is fixed and is provided with tray (2), and the top of tray (2) settles and have cell culture dish (3), platform (5) set up in the top of cell culture dish (3), and the top of platform (5) is provided with connecting pipe (6), mouth of pipe connection structure (14) set up in the upper end of connecting pipe (6), and the upper end of mouth of pipe connection structure (14) is connected with ordinary drainage tube (9), the up end of household utensils (1) is provided with apron (10), and the left side of apron (10) is connected with hinge (15) to the up end of apron (10) is fixed with handle (11).

2. The tumor-shed cell enrichment and primary culture drainage device of claim 1, wherein: the right side of the vessel (1) is connected with a connecting pipeline (12), and the right side of the connecting pipeline (12) is connected with a negative pressure device (13).

3. The tumor-shed cell enrichment and primary culture drainage device of claim 1, wherein: the utensil (1) is communicated with the negative pressure device (13) through the connecting pipeline (12), and the cover plate (10) forms a rotating structure with the utensil (1) through the hinge (15).

4. The tumor-shed cell enrichment and primary culture drainage device of claim 1, wherein: side holes (4) are formed in the side faces of the cell culture dish (3), and the distance between each side hole (4) and the bottom end of the cell culture dish (3) is 3-4 mm.

5. The tumor-shed cell enrichment and primary culture drainage device of claim 1, wherein: the middle of the connecting pipe (6) is provided with a transfer ball (7), a cell filter screen (8) is arranged inside the transfer ball (7), and the diameter of the cell filter screen (8) is 40 um.

6. The tumor-shed cell enrichment and primary culture drainage device of claim 1, wherein: the pipe orifice connecting structure (14) comprises a first connecting thread (141), a connecting raised head (142) and a second connecting thread (143), the first connecting thread (141) is arranged inside the tail end of the common drainage pipe (9), the connecting raised head (142) is arranged at the top end of the connecting pipe (6), the second connecting thread (143) is arranged on the outer surface of the connecting raised head (142), and the connecting pipe (6) is in threaded connection with the common drainage pipe (9) through the pipe orifice connecting structure (14).