CN213048729U - Micro-injection device - Google Patents
Micro-injection device Download PDFInfo
- Publication number
- CN213048729U CN213048729U CN202021601583.0U CN202021601583U CN213048729U CN 213048729 U CN213048729 U CN 213048729U CN 202021601583 U CN202021601583 U CN 202021601583U CN 213048729 U CN213048729 U CN 213048729U
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- CN
- China
- Prior art keywords
- micro
- glass
- glass microelectrode
- extension tube
- microelectrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000520 microinjection Methods 0.000 title claims abstract description 29
- 239000011521 glass Substances 0.000 claims abstract description 58
- 210000004556 brain Anatomy 0.000 abstract description 12
- 230000006378 damage Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003625 skull Anatomy 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 208000018380 Chemical injury Diseases 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000006931 brain damage Effects 0.000 description 1
- 231100000874 brain damage Toxicity 0.000 description 1
- 208000029028 brain injury Diseases 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The utility model discloses a micro-injection device, belonging to the field of medical equipment, comprising a micro-injector, an extension tube and a glass microelectrode; the needle point at the front end of the micro-syringe is arranged in an extension tube, and a glass microelectrode formed by drawing a glass capillary tube is arranged in the other end of the extension tube; the micro-injector is vertically fixed on a stereo positioning instrument or transversely placed on a micro-injection pump, and the glass microelectrode is fixed at the bottom end of a vertical operating arm of the stereo positioning instrument. The utility model discloses a microinjection device combines together microinjector and glass microelectrode, because the glass characteristic that the glass microelectrode has, can be straight promptly, can not be used promptly brittle failure, ensured the accurate nature of location, reduced the tradition and only used the positioning error that the microinjector brought, and the superfine pointed end of the glass microelectrode by glass capillary drawing has reduced the damage of microinjection to the mouse brain greatly, has reduced the operation requirement to the microinjector simultaneously, can recycle, and is not fragile.
Description
Technical Field
The utility model relates to a micro-injection device belongs to the medical equipment field.
Background
Neuroscience is a science that studies the nature and regularity of cognition, consciousness and intelligence of humans, animals and machines. As the research of brain science and brain-like science becomes the central importance of the science and technology strategy of Chinese brain planning, the brain also becomes the most attention-focused research object in neuroscience. In the basic research of neuroscience, the accuracy degree of brain stereotactic is directly related to the authenticity of an experimental result, so that high-accuracy positioning becomes a basic but crucial operation skill in the current neuroscience field, and the accuracy of continuous optimization positioning has important scientific research significance.
In the conventional stereotactic technique for brain, microinjection is generally performed by using a microinjector, which can be fixed on a vertical operating arm of a stereotaxic apparatus or a microinjection pump (abbreviated as a micropump) to directly or indirectly (such as an extension tube) administer the drug. The microinjector has the advantages of low cost, small injection amount, adjustable injection amount and the like, is widely applied, but has obvious defects, such as overlarge diameter of a needle end entering the brain, easy infection after repeated use and easy bending of a needle point, and increases mechanical and chemical injuries to the brain.
In electrophysiology, glass microelectrodes play a significant role due to their excellent sealing and electrochemical properties. Although the tip of the glass microelectrode drawn by the hard capillary can reach the micron or nanometer level, the glass microelectrode is not paid enough attention in the brain microinjection experiment, and the application of the glass microelectrode is further limited by the high cost of a power source glass microelectrode propeller.
Disclosure of Invention
To the problem that above-mentioned prior art exists, the utility model provides a microinjection device, with low costs, simple process, the security is high, can effectively reduce the brain damage, is showing the degree of accuracy that improves the location.
In order to realize the purpose, the utility model discloses a micro-injection device, which comprises a micro-injector, an extension tube connected with the micro-injector, and a glass microelectrode connected with the extension tube;
the needle point at the front end of the micro-syringe is arranged in an extension tube, and a glass microelectrode formed by drawing a glass capillary tube is arranged in the other end of the extension tube;
the micro-injector is vertically fixed on a stereo positioning instrument or transversely placed on a micro-injection pump, and the glass microelectrode is fixed at the bottom end of a vertical operating arm of the stereo positioning instrument.
As an improvement, the extension pipe adopts a hose.
As an improvement, the diameter of the cross section of the extension tube is larger than the diameter of the connecting end of the glass microelectrode and the diameter of the needle tip of the micro-syringe.
Compared with the prior art, the utility model discloses a microinjection device combines together microinjector and glass microelectrode, because glass microelectrode has the glass characteristic, can be straight, can not be used promptly brittle failure, has ensured the accurate nature of location, has reduced the positioning error that the tradition only used the microinjector to bring, and the superfine pointed end of the glass microelectrode by glass capillary drawing has just significantly reduced the damage of microinjection to the mouse brain, simultaneously the utility model discloses greatly reduced to the operation requirement of microinjector, make the microinjector can recycle, it is not fragile.
Drawings
Fig. 1 is a schematic view of a usage state of the present invention;
fig. 2 is a schematic view of another usage state of the present invention;
in the figure: 1. a micro-injector, 11, a needle tip, 2, an extension tube, 3, a glass microelectrode, 4 and a micro-injection pump.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below. It should be understood, however, that the description herein of specific embodiments is only intended to illustrate the invention and not to limit the scope of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention.
As shown in FIGS. 1 and 2, a micro-injection device includes a micro-syringe 1, an extension tube 2 connected to the micro-syringe 1, and a glass microelectrode 3 connected to the extension tube 2;
a needle point 11 at the front end of the micro-syringe 1 is arranged in an extension tube 2, and a glass microelectrode 3 formed by drawing a glass capillary is arranged in the other end of the extension tube 2;
the micro-injector 1 is vertically fixed on a stereo positioning instrument or transversely placed on a micro-injection pump 4, and the glass microelectrode 3 is fixed at the bottom end of a vertical operating arm of the stereo positioning instrument.
Example 1
As shown in FIG. 1, the micro-injection device of the present invention has the following operation process in the vertical method:
(1) preparing an electrode: drawing a glass capillary tube by using a microelectrode drawing instrument, adjusting parameters, drawing one glass capillary tube into two glass microelectrodes 3 with slightly pointed ends, slightly and vertically cutting off burrs at the tip of the glass microelectrodes by using a sterilization blade, and enabling the length of the tip of the glass microelectrodes 3 to meet the experimental requirements;
(2) connecting an extension pipe and filling the pipe: the extension tube 2 adopts a section of flexible tube, the diameter of the cross section of the flexible tube is slightly larger than the diameter of the connecting tip of the glass microelectrode 3 and the diameter of the needlepoint 11 of the micro-injector 1, the connecting tip rancour of the glass microelectrode 3 is gently put into the extension tube 2 by hand (the extension tube 2 can be firstly expanded by sharp objects such as a 10ul gun head and the like so as to be convenient for operation), then the micro-injector 1 absorbs sufficient 0.01M sterile PBS solution, and the sterile PBS solution is injected into the glass microelectrode 3 through the extension tube 2, so that the whole pipeline is filled with liquid;
(3) fixing an apparatus: the glass microelectrode 3 is directly fixed at the bottom end of a vertical operating arm of the stereotaxic apparatus, and the micro-injector 1 can be bound at any position of the stereotaxic apparatus convenient to be pushed by using a sleeve rubber band;
(4) bregma point determination: after a mouse is subjected to a series of operations such as anesthesia, craniocerebral fixation, skin preparation, bregma exposure and the like, the operation arm of the stereotaxic instrument is adjusted until the glass microelectrode 3 is positioned above the bregma (the X axis and the Y axis are determined), the microinjector 1 is slightly pushed until a small drop of solution is exposed from the tip of the glass microelectrode 3, and the solution is immediately diffused when touching the surface of the skull, so that the zero point of the Z axis is judged, and the problem that the tip is easy to break is simply and easily avoided (the operation can be carried out under an optical microscope);
(5) microinjection: after the skull is broken through electric drilling according to the coordinates of a target nucleus, slightly pushing the micro-syringe 1 to ensure that a solution flows out from the tip of the glass microelectrode 3 and the passage is smooth, sucking a small amount of negative pressure backwards to reserve a section of air phase, sucking other solutions such as liquid medicine or virus suspension liquid which is slightly higher than the injection amount and needs to be injected into the brain, confirming the smooth passage again, downwards rotating the tip of the glass microelectrode 3 to the target nucleus, adjusting the propelling speed of the micro-syringe 1 according to the experimental needs, rotating to half, reserving a needle for 5-10 min, and then continuing propelling;
(6) cleaning and maintaining: after the administration is finished, the glass microelectrode 3 is screwed out, the microinjector 1 is rapidly and vigorously pushed to wash the tube, so that blood, brain tissues and other substances at the tip port of the glass microelectrode 3 are timely washed away, the pipeline is ensured to be smooth, a proper amount of alcohol is dipped by a clean cotton swab to slightly wipe the outside of the tip end of the glass microelectrode 3, the bottom end is slightly wiped, the next nuclear group positioning experiment can be continuously carried out after the alcohol is dried, the glass microelectrode 3 is stored in a self-made microelectrode storage box (the original storage box of the glass capillary tube, the shockproof sponge is cut to a plurality of thin strips with the height of 1/2 by a blade), the glass microelectrode 3 can be soaked in boiling water for sterilization before the next use, and the use is continued after the alcohol wiping.
Example 2
As shown in FIG. 2, the micro-injection device of the present invention operates in a horizontal mode as in a vertical mode, but the micro-injection pump 4 is used to replace the manual micro-injector 1, so that a small amount of fluid can be accurately, micro-uniformly and continuously output, and the micro-injection device has more accurate control capability.
The utility model discloses a microinjection device combines together microinjector and glass microelectrode, because the glass characteristic that the glass microelectrode has, available promptly is straight, can not be used promptly brittle failure, has ensured the accurate nature of location, has reduced the positioning error that the tradition only used the microinjector to bring, and the superfine pointed end of the glass microelectrode by glass capillary drawing has just significantly reduced the damage of microinjection to mouse brain, simultaneously the utility model discloses greatly reduced to the operation requirement of microinjector, make the microinjector can recycle, it is not fragile.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modification, equivalent replacement or improvement made within the spirit and principle of the present invention should be included in the present invention.
Claims (3)
1. A micro-injection device is characterized by comprising a micro-injector (1), an extension tube (2) connected with the micro-injector (1), and a glass microelectrode (3) connected with the extension tube (2);
a needle point (11) at the front end of the micro-syringe (1) is arranged in an extension tube (2), and a glass microelectrode (3) formed by drawing a glass capillary tube is arranged in the other end of the extension tube (2);
the micro-injector (1) is vertically fixed on a stereo positioning instrument or transversely placed on a micro-injection pump (4), and the glass microelectrode (3) is fixed at the bottom end of a vertical operating arm of the stereo positioning instrument.
2. A micro-injection device according to claim 1, wherein the extension tube (2) is a flexible tube.
3. A micro-injection device according to claim 1 or 2, wherein the cross-sectional diameter of the extension tube (2) is larger than the diameter of the connection end of the glass microelectrode (3) and the diameter of the tip (11) of the micro-syringe (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021601583.0U CN213048729U (en) | 2020-08-05 | 2020-08-05 | Micro-injection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021601583.0U CN213048729U (en) | 2020-08-05 | 2020-08-05 | Micro-injection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213048729U true CN213048729U (en) | 2021-04-27 |
Family
ID=75580022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021601583.0U Expired - Fee Related CN213048729U (en) | 2020-08-05 | 2020-08-05 | Micro-injection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213048729U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115305182A (en) * | 2022-07-29 | 2022-11-08 | 北京脑科学与类脑研究中心 | Micro-drug delivery device and control system |
-
2020
- 2020-08-05 CN CN202021601583.0U patent/CN213048729U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115305182A (en) * | 2022-07-29 | 2022-11-08 | 北京脑科学与类脑研究中心 | Micro-drug delivery device and control system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210427 |