CN212981096U

CN212981096U - Immune cell cold storage device

Info

Publication number: CN212981096U
Application number: CN202021418183.6U
Authority: CN
Inventors: 袁牧; 孙云哲
Original assignee: Weihai Jiansheng Biotechnology Co ltd
Current assignee: Weihai Jiansheng Biotechnology Co ltd
Priority date: 2020-07-17
Filing date: 2020-07-17
Publication date: 2021-04-16
Anticipated expiration: 2030-07-17

Abstract

The utility model belongs to the technical field of cell storage, and particularly discloses an immune cell cold storage device, which comprises a shell, a cold storage chamber, a tool chamber and a cover plate; a partition plate is vertically arranged in the center of the inside of the shell; a cold storage chamber is arranged on one side of the partition plate, and a tool chamber is arranged on the other side of the partition plate; a liquid nitrogen layer is arranged at the bottom end of the inner part of the cold storage chamber, and clamping blocks are fixed on two sides of the inner part of the cold storage chamber above the liquid nitrogen layer; the top end of the shell is provided with a cover plate; the bottom surface of the cover plate is fixedly connected with a sealing frame, the size of the sealing frame is smaller than that of the shell, a first magnetic sheet is fixedly bonded on the outer side of the sealing frame, a second magnetic sheet is fixedly bonded on the inner wall of the top end of the shell, and the sealing frame is magnetically connected with the shell through the matching of the first magnetic sheet and the second magnetic sheet; the top surface of the shell is fixedly bonded with a first sealing strip, the bottom surface of the cover plate is provided with a groove corresponding to the position of the first sealing strip, a second sealing strip is arranged in the groove, and the top end of the second sealing strip is fixedly connected with a plurality of extrusion springs.

Description

Immune cell cold storage device

Technical Field

The utility model relates to a technical field is stored to the cell, specifically is an immune cell cold storage device.

Background

The method for preserving cells by utilizing the liquid nitrogen deep low temperature (-196 ℃) environment is a cell long-term preservation technology which is generally used in the world at present. The principle is that the metabolism of cells can be interrupted in a deep low temperature environment. However, the temperature decrease rate during freezing and the temperature increase rate during cell thawing have a great influence on the preservation of cell viability. When the conditions are proper, the frozen cells are recovered and the 37' C culture is recovered, and the morphology and the metabolic activity of the cells can be recovered to be normal.

The conventional immune cell freezing and storing device is not provided with a transition structure, so that immune cells are easy to inactivate during freezing, and the conventional storing device is only sealed by a simple sealing strip, so that the sealing effect is poor after long-term use, and the heat preservation is influenced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an immune cell cold storage device to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: an immune cell cold storage device comprises a shell, a cold storage chamber, a tool chamber and a cover plate; a partition plate is vertically arranged in the center of the inside of the shell; a cold storage chamber is arranged on one side of the partition plate, and a tool chamber is arranged on the other side of the partition plate; a liquid nitrogen layer is arranged at the bottom end of the inner part of the cold storage chamber, and clamping blocks are fixed on two sides of the inner part of the cold storage chamber above the liquid nitrogen layer; the top end of the shell is provided with a cover plate; the bottom surface of the cover plate is fixedly connected with a sealing frame, the size of the sealing frame is smaller than that of the shell, a first magnetic sheet is fixedly bonded on the outer side of the sealing frame, a second magnetic sheet is fixedly bonded on the inner wall of the top end of the shell, and the sealing frame is magnetically connected with the shell through the matching of the first magnetic sheet and the second magnetic sheet; the improved sealing structure is characterized in that a first sealing strip is fixedly bonded to the top surface of the shell, a groove is formed in the bottom surface of the cover plate corresponding to the first sealing strip, a second sealing strip is arranged in the groove, a plurality of extrusion springs are fixedly connected to the top end of the second sealing strip, and the other ends of the extrusion springs are fixedly connected with the inner wall of the groove.

Preferably, the cross section of the first sealing strip is in a positive E shape, the cross section of the second sealing strip is in an inverted E shape, and the first sealing strip and the second sealing strip are matched with each other.

Preferably, the surface of the sealing frame is provided with an opening at a position corresponding to the partition plate, and the size of the opening is matched with that of the partition plate.

Preferably, a cold storage pipe frame is horizontally arranged between the two clamping blocks, the cold storage pipe frame is fixedly clamped with the two clamping blocks, and a plurality of cold storage pipes are arranged in the cold storage pipe frame; when using, the staff wears to take out from the instrument room behind the gloves that prevent frostbite, makes immunocyte and culture solution separation through centrifugal action with the immunocyte, puts into cold save intraductal and add DMSO and be used for cold deposit with the immunocyte, later with cold save the pipe put into cold save the pipe support in and will cold save the pipe support and be fixed in two fixture block surfaces, cold save the pipe support and fix with two fixture block accessible blocks, thereby play the effect of cooling transition to cold save the pipe, later put into the liquid nitrogen in situ with cold save the pipe support and carry out cold save.

Preferably, the bottom end of the partition board is provided with a liquid nitrogen supplementing port, and the liquid nitrogen supplementing port is plugged through a sealing plug; the liquid nitrogen layer in the cold storage chamber can be supplemented through a liquid nitrogen supplementing port.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses be provided with the fixture block in liquid nitrogen layer top both sides, two fixture block surface levels are provided with the cold pipe support of depositing, and the cold pipe support of depositing is fixed in two fixture block surfaces through the block to give a transitional action of immunocyte, prevent that immunocyte from leading to the condition of inactivation because the abrupt change of temperature.

The utility model discloses a magnetism between sealed frame and the casing is connected and is made apron and casing closed, seals through the cooperation of first sealing strip and second sealing strip again, a plurality of extrusion springs of second sealing strip top fixedly connected with, and the sealed effect of first sealing strip and second sealing strip can be strengthened to the extrusion spring to reach heat retaining purpose, finally guarantee that immune cell can freeze for a long time and store.

Drawings

FIG. 1 is a schematic view of the connection of the whole of the present invention;

fig. 2 is an overall front view of the present invention;

fig. 3 is a schematic view of the three-dimensional structure of the cover plate of the present invention.

In the figure: 1. a housing; 2. a cold storage chamber; 3. a tool chamber; 4. a partition plate; 5. a liquid nitrogen layer; 6. a clamping block; 7. a cover plate; 8. a sealing frame; 9. a first magnetic sheet; 10. a second magnetic sheet; 11. a first seal strip; 12. a second seal strip; 13. a groove; 14. a compression spring; 15. an opening; 16. cold storage of the pipe frame; 17. and a liquid nitrogen supplement port.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

Referring to fig. 1-3, the present invention provides a technical solution: an immune cell cold storage device comprises a shell 1, a cold storage chamber 2, a tool chamber 3 and a cover plate 7; a partition plate 4 is vertically arranged at the center inside the shell 1; a cold storage chamber 2 is arranged on one side of the partition plate 4, and a tool chamber 3 is arranged on the other side of the partition plate 4; a liquid nitrogen layer 5 is arranged at the bottom end inside the cold storage chamber 2, and clamping blocks 6 are fixed on two sides of the inside of the cold storage chamber 2 above the liquid nitrogen layer 5; a cover plate 7 is arranged at the top end of the shell 1; the bottom surface of the cover plate 7 is fixedly connected with a sealing frame 8, the size of the sealing frame 8 is smaller than that of the shell 1, a first magnetic sheet 9 is fixedly bonded on the outer side of the sealing frame 8, a second magnetic sheet 10 is fixedly bonded on the inner wall of the top end of the shell 1, and the sealing frame 8 is magnetically connected with the shell 1 through the matching of the first magnetic sheet 9 and the second magnetic sheet 10; the top surface of the shell 1 is fixedly bonded with a first sealing strip 11, the bottom surface of the cover plate 7 is provided with a groove 13 corresponding to the position of the first sealing strip 11, a second sealing strip 12 is arranged in the groove 13, the top end of the second sealing strip 12 is fixedly connected with a plurality of extrusion springs 14, and the other ends of the extrusion springs 14 are fixedly connected with the inner wall of the groove 13.

Further, the first sealing tape 11 has a positive "E" shape in cross section, the second sealing tape 12 has an inverted "E" shape in cross section, and the first sealing tape 11 and the second sealing tape 12 are fitted to each other.

Furthermore, an opening 15 is formed in the surface of the sealing frame 8 at a position corresponding to the partition plate 4, and the size of the opening 15 is matched with that of the partition plate 4.

Further, a cold storage pipe frame 16 is horizontally arranged between the two fixture blocks 6, the cold storage pipe frame 16 and the two fixture blocks 6 are fixed in a clamping manner, and a plurality of cold storage pipes are arranged in the cold storage pipe frame 16; when using, the staff wears to take out behind the gloves that prevent frostbite from instrument room 3, make immunocyte and culture solution separation through centrifugal action with immunocyte, put into cold storage intraductal and add DMSO and be used for cold depositing with immunocyte, later deposit the pipe with cold and put into cold storage pipe support and be fixed in two fixture block 6 surfaces with cold storage pipe support, cold storage pipe support 16 is fixed with 6 accessible blocks of two fixture block, thereby play the effect of cooling transition to cold storage pipe, later deposit cold storage pipe support 16 again and carry out cold storage in putting into liquid nitrogen layer 5.

Further, a liquid nitrogen supplement port 17 is formed in the bottom end of the partition plate 4, and the liquid nitrogen supplement port 17 is plugged through a sealing plug; the liquid nitrogen layer 5 in the cold storage chamber 2 can be supplemented through a liquid nitrogen supplement port 17.

The utility model provides an immune cell cold storage device, including casing 1, cold room 2, tool room 3 and apron 7 of depositing, casing 1 inside center is vertical to be provided with baffle 4, baffle 4 one side is provided with cold room 2 of depositing, baffle 4 opposite side is provided with tool room 3, cold room 2 bottom of depositing is provided with liquid nitrogen layer 5, cold room 2 inside is located liquid nitrogen layer 5 top both sides and is fixed with fixture block 6, be provided with cold pipe frame 16 between two fixture block 6, cold pipe frame 16 inside is provided with a plurality of cold pipes of depositing, the immune cell is put into cold when needing to preserve in depositing intraductally, place cold pipe in cold pipe frame 16 inside, cold pipe frame 16 is fixed in two fixture block 6 surfaces through the block, thereby a transitional action is given to the immune cell, later place cold pipe inside the liquid nitrogen again and preserve, thereby realize the long-term preservation of immune cell; a tool chamber 3 is arranged on one side of the cold storage chamber 2, and immune cell storage tools are stored in the tool chamber 3 and are convenient for personnel to use;

the top end of the shell 1 is provided with a cover plate 7, the bottom surface of the cover plate 7 is fixedly connected with a sealing frame 8, the size of the sealing frame 8 is smaller than that of the shell 1, a first magnetic sheet 9 is fixedly bonded on the outer side of the sealing frame 8, a second magnetic sheet 10 is fixedly bonded on the inner wall of the top end of the shell 1, the sealing frame 8 is magnetically connected with the shell 1 through the matching of the first magnetic sheet 9 and the second magnetic sheet 10, a first sealing strip 11 is fixedly bonded on the top surface of the shell 1, a groove 13 is formed in the position, corresponding to the first sealing strip 11, of the bottom surface of the cover plate 7, a second sealing strip 12 is arranged inside the groove 13, the top end of the second sealing strip 12; when the cover plate 7 is closed, the magnetic connection between the sealing frame 8 and the shell 1 is fixed, then the sealing is performed by the matching of the first sealing strip 11 and the second sealing strip 12, the top end of the second sealing strip 12 is fixedly connected with a plurality of extrusion springs 14, and the extrusion springs 14 can strengthen the sealing effect of the first sealing strip 11 and the second sealing strip 12, so that the purpose of heat preservation is achieved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An immune cell cryopreservation device, comprising: comprises a shell (1), a cold storage chamber (2), a tool chamber (3) and a cover plate (7); a partition plate (4) is vertically arranged at the center inside the shell (1); a cold storage chamber (2) is arranged on one side of the partition plate (4), and a tool chamber (3) is arranged on the other side of the partition plate (4); a liquid nitrogen layer (5) is arranged at the bottom end inside the cold storage chamber (2), and clamping blocks (6) are fixed on two sides of the inside of the cold storage chamber (2) above the liquid nitrogen layer (5); a cover plate (7) is arranged at the top end of the shell (1); the bottom surface of the cover plate (7) is fixedly connected with a sealing frame (8), the size of the sealing frame (8) is smaller than that of the shell (1), a first magnetic sheet (9) is fixedly bonded on the outer side of the sealing frame (8), a second magnetic sheet (10) is fixedly bonded on the inner wall of the top end of the shell (1), and the sealing frame (8) is magnetically connected with the shell (1) through the matching of the first magnetic sheet (9) and the second magnetic sheet (10); the sealing device is characterized in that a first sealing strip (11) is fixedly bonded to the top surface of the shell (1), a groove (13) is formed in the position, corresponding to the first sealing strip (11), of the bottom surface of the cover plate (7), a second sealing strip (12) is arranged inside the groove (13), a plurality of extrusion springs (14) are fixedly connected to the top end of the second sealing strip (12), and the other ends of the extrusion springs (14) are fixedly connected with the inner wall of the groove (13).

2. The immune cell cryopreservation device of claim 1, wherein: the section of the first sealing strip (11) is in a positive E shape, the section of the second sealing strip (12) is in an inverted E shape, and the first sealing strip (11) and the second sealing strip (12) are matched with each other.

3. The immune cell cryopreservation device of claim 1, wherein: an opening (15) is formed in the position, corresponding to the partition plate (4), of the surface of the sealing frame (8), and the size of the opening (15) is matched with that of the partition plate (4).

4. The immune cell cryopreservation device of claim 1, wherein: two be provided with cold pipe support (16) between fixture block (6) the level, cold pipe support (16) and two fixture block (6) are fixed through the block, and cold inside a plurality of cold pipes of depositing pipe support (16).

5. The immune cell cryopreservation device of claim 1, wherein: and a liquid nitrogen supplement port (17) is formed in the bottom end of the partition plate (4), and the liquid nitrogen supplement port (17) is plugged through a sealing plug.