CN212980732U - Sodium citrate buffer solution preparation kit - Google Patents

Sodium citrate buffer solution preparation kit Download PDF

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Publication number
CN212980732U
CN212980732U CN202021841191.1U CN202021841191U CN212980732U CN 212980732 U CN212980732 U CN 212980732U CN 202021841191 U CN202021841191 U CN 202021841191U CN 212980732 U CN212980732 U CN 212980732U
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China
Prior art keywords
buffer solution
bottle lid
straw
sodium citrate
citrate buffer
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CN202021841191.1U
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Chinese (zh)
Inventor
姚远颋
许舟
顾重建
李克强
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Chongqing Wokang Biological Technology Co ltd
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Chongqing Wokang Biological Technology Co ltd
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Priority to CN202021841191.1U priority Critical patent/CN212980732U/en
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Abstract

The utility model discloses a sodium citrate buffer solution configuration kit, including the body, the top threaded connection on body surface has the bottle lid, the right side of bottle lid is provided with the straw, one side that the straw is close to the body runs through to the inside of body, the bleeder vent has been seted up to the inside of bottle lid, the top of bottle lid inner wall is provided with the flexible glue pad that is located the bleeder vent bottom. The utility model discloses a set up the body, the bottle lid, the straw, the bleeder vent, the cooperation of soft rubber pad and scale mark is used, the user extrudees the body gently, because the bleeder vent has been sealed to the soft rubber pad, liquid can flow through the straw, then according to required volume, take the buffer solution volume according to the scale on the body, it is powder basically to have solved current sodium citrate buffer solution, so the process of configuration is comparatively complicated, and just join in marriage now, can't maintain the stability of pH for a long time, need split packing into after the small package, dispose respectively, just so caused the extravagant problem in time.

Description

Sodium citrate buffer solution preparation kit
Technical Field
The utility model relates to a sodium citrate buffer solution technical field specifically is sodium citrate buffer solution configuration kit.
Background
The sodium citrate buffer solution is 0.01mol/L, the pH value is 6.0, the sodium citrate buffer solution is a commonly used antigen repair solution, can be used for antigen repair of samples such as paraffin sections, frozen sections and the like after being fixed by paraformaldehyde, formaldehyde or other aldehyde reagents, can effectively remove cross-linking among proteins caused by the aldehyde fixing reagents, and fully expose antigen epitopes in the samples such as the paraffin sections and the like, thereby greatly improving the immunostaining effect.
However, the existing sodium citrate buffer solution is basically powder, so the preparation process is complex, the buffer solution can not maintain the stability of pH for a long time when being prepared for use, and the buffer solution needs to be prepared respectively after being subpackaged into small packages, thereby causing the waste of time and being inconvenient for users to use.
SUMMERY OF THE UTILITY MODEL
For solving the problem that provides among the above-mentioned background art, the utility model aims to provide a sodium citrate buffer solution configuration kit possesses and avoids uncapping and cause secondary pollution, and the sample is accurate to the milliliter, and easy operation is convenient, uses the nimble advantage of scene, has solved current sodium citrate buffer solution and has basically been the powder, so the process of configuration is comparatively complicated to the existing use of joining in marriage, can't maintain the stability of pH for a long time, need the partial shipment become the little packing after, dispose respectively, just so caused the extravagant problem in time.
In order to achieve the above object, the utility model provides a following technical scheme: sodium citrate buffer solution configuration kit, including the body, the top threaded connection on body surface has the bottle lid, the right side of bottle lid is provided with the straw, one side that the straw is close to the body runs through to the inside of body, the bleeder vent has been seted up to the inside of bottle lid, the top of bottle lid inner wall is provided with the flexible glue pad that is located the bleeder vent bottom, the right side at flexible glue pad top and the inner wall fixed connection of bottle lid, the scale mark has been seted up on the surface of body.
As the utility model discloses it is preferred, the top and the bottom on body surface all are the round chamfer setting, the top and the bottom on bottle lid surface all are the round chamfer setting.
As the utility model discloses preferred, the material of body is made for the PVC material, the material of straw is made for PVC.
As the utility model discloses preferred, soft cushion is the setting of circle shape, the diameter of soft cushion is greater than the diameter of bleeder vent.
As the utility model discloses preferred, the scale mark is located the positive left side of body, the total height of scale mark is two hundred milliliters.
As the utility model discloses preferred, the surface cover of straw is equipped with the sealing washer, the left side of sealing washer and the right side fixed connection of bottle lid.
Compared with the prior art, the beneficial effects of the utility model are as follows:
1. the utility model discloses a set up the body, the bottle lid, the straw, the bleeder vent, the cooperation of soft cushion and scale mark is used, it is the powder basically to have solved current sodium citrate buffer solution, so the process of configuration is comparatively complicated, and just join in marriage usefulness now, can't maintain the stability of pH for a long time, need the partial shipment one-tenth after the small packaging, the configuration respectively, just so caused the extravagant problem in time, this sodium citrate buffer solution configuration kit, possess and avoid uncapping and cause secondary pollution, the accurate milliliter that arrives of sample, and easy operation is convenient, use scene nimble advantage, convenient to use person's use.
2. The utility model discloses a body and bottle lid are the setting of round chamfer, can play the effect of protection when the user uses, prevent that the closed angle of body and bottle lid from causing the injury to the user, and the setting of round chamfer can improve the pleasing to the eye of body and bottle lid moreover.
3. The utility model discloses a setting of body and straw material, the person of facilitating the use extrudes the body and makes reagent flow through the straw, has improved the convenience that the user used the body.
4. The utility model discloses a setting of soft cushion can seal the bleeder vent when not using the kit, prevents that external impurity from getting into inside the body through the bleeder vent.
5. The utility model discloses a set up the scale mark, the person of facilitating the use accurately masters the weight of pouring out reagent through the straw, can not cause the chaotic problem of configuration.
6. The utility model discloses a set up the sealing washer, can fill the gap that forms between straw and the bottle lid, prevent effectively that external impurity from getting into the body through the gap inside to cause the influence to the inside reagent of body.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a perspective view of the bottle body of the present invention;
FIG. 3 is an enlarged view of the structure A of FIG. 1 according to the present invention;
fig. 4 is a top view of the present invention.
In the figure: 1. a bottle body; 2. a bottle cap; 3. a straw; 4. air holes are formed; 5. a soft rubber cushion; 6. scale lines; 7. and (5) sealing rings.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1 to 4, the utility model provides a sodium citrate buffer solution configuration kit, including body 1, the top threaded connection on body 1 surface has bottle lid 2, and the right side of bottle lid 2 is provided with straw 3, and straw 3 runs through to the inside of body 1 near one side of body 1, and bleeder vent 4 has been seted up to the inside of bottle lid 2, and the top of 2 inner walls of bottle lid is provided with the soft rubber pad 5 that is located bleeder vent 4 bottom, and the right side at 5 tops of soft rubber pad and the inner wall fixed connection of bottle lid 2, and scale mark 6 has been seted up on the surface of body 1.
Referring to fig. 1, the top and the bottom of the surface of the bottle body 1 are both arranged in a round chamfer, and the top and the bottom of the surface of the bottle cap 2 are both arranged in a round chamfer.
As a technical optimization scheme of the utility model, be the setting of round chamfer through body 1 and bottle lid 2, can play the effect of protection when the user uses, prevent that the closed angle of body 1 and bottle lid 2 from causing the injury to the user, the setting of round chamfer can improve the pleasing to the eye of body 1 and bottle lid 2 moreover.
Referring to fig. 1, the bottle body 1 is made of PVC, and the straw 3 is made of PVC.
As the utility model discloses a technical optimization scheme, through the setting of body 1 and 3 materials of straw, the person of facilitating the use extrudes body 1 and makes reagent flow out through straw 3, has improved the convenience that the user used body 1.
Referring to fig. 3, the soft rubber pad 5 is arranged in a circular shape, and the diameter of the soft rubber pad 5 is larger than that of the ventilation hole 4.
As a technical optimization scheme of the utility model, through the setting of soft cushion 5, can seal bleeder vent 4 when not using the kit, prevent that external impurity from getting into 1 insidely of body through bleeder vent 4.
Referring to fig. 1 and 2, the graduation marks 6 are located at the left side of the front surface of the body 1, and the total height of the graduation marks 6 is two hundred milliliters.
As a technical optimization scheme of the utility model, through setting up scale mark 6, the person of facilitating the use accurately masters the weight of pouring out reagent through straw 3, can not cause the chaotic problem of configuration.
Referring to fig. 1, a sealing ring 7 is sleeved on the surface of the suction pipe 3, and the left side of the sealing ring 7 is fixedly connected with the right side of the bottle cap 2.
As a technical optimization scheme of the utility model, through setting up sealing washer 7, can fill the gap that forms between straw 1 and the bottle lid 2, prevent effectively that external impurity from getting into body 1 inside through the gap to cause the influence to body 1 inside reagent.
The utility model discloses a theory of operation and use flow: when in use, a user firstly adds sodium citrate buffer solution powder into a bottle body 1, then adds two hundred milliliters of purified water, then rotates a bottle cap 2 to enable the bottle cap 2 to be matched and sealed with the bottle body 1, then slightly squeezes the bottle body 1, because a soft rubber pad 5 seals a vent hole 4, liquid can flow out through a suction pipe 3, the user can take the buffer solution according to the required amount and the scale mark 6 on the bottle body 1, after the buffer solution is taken, air can push the soft rubber pad 5 open from the vent hole 4 and enter the bottle body 1, the product can be prepared into the corresponding volume solution according to a package mark in the taking process, 1L of buffer solution can be prepared for every 50ml, 4L of buffer solution can be prepared for every 200ml of bottle, (if necessary, 20 can be added according to requirements), after cells or tissues are fixed by formalin (or paraformaldehyde and formaldehyde reagents), the cross-linking between proteins can be caused, therefore, the antigen epitope is shielded, the immune staining result is influenced, the antigen repairing buffer solution adopts the antigen heat repairing technical principle, and in the heating process, the cross-linking between proteins is opened, so that the antigen epitope is fully exposed to achieve the purpose of antigen repairing.
In summary, the following steps: this sodium citrate buffer solution configuration kit uses through the cooperation that sets up body 1, bottle lid 2, straw 3, bleeder vent 4, soft cushion 5 and scale mark 6, has solved current sodium citrate buffer solution and is the powder basically, so the process of configuration is comparatively complicated to the existing use of joining in marriage, can't maintain the stability of pH for a long time, need split charging become the little packing after, dispose respectively, just so caused the extravagant problem in time.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. Sodium citrate buffer solution configuration kit, including body (1), its characterized in that: the top threaded connection on body (1) surface has bottle lid (2), the right side of bottle lid (2) is provided with straw (3), one side that straw (3) are close to body (1) runs through to the inside of body (1), bleeder vent (4) have been seted up to the inside of bottle lid (2), the top of bottle lid (2) inner wall is provided with soft cushion (5) that are located bleeder vent (4) bottom, the right side at soft cushion (5) top and the inner wall fixed connection of bottle lid (2), scale mark (6) have been seted up on the surface of body (1).
2. The sodium citrate buffer solution preparation kit according to claim 1, characterized in that: the top and the bottom on body (1) surface all are round chamfer setting, the top and the bottom on bottle lid (2) surface all are round chamfer setting.
3. The sodium citrate buffer solution preparation kit according to claim 1, characterized in that: the bottle body (1) is made of PVC materials, and the straw (3) is made of PVC materials.
4. The sodium citrate buffer solution preparation kit according to claim 1, characterized in that: the soft rubber cushion (5) is arranged in a circular shape, and the diameter of the soft rubber cushion (5) is larger than that of the air holes (4).
5. The sodium citrate buffer solution preparation kit according to claim 1, characterized in that: the scale mark (6) is positioned on the left side of the front face of the bottle body (1), and the total height of the scale mark (6) is two hundred milliliters.
6. The sodium citrate buffer solution preparation kit according to claim 1, characterized in that: the surface cover of straw (3) is equipped with sealing washer (7), the left side of sealing washer (7) and the right side fixed connection of bottle lid (2).
CN202021841191.1U 2020-08-28 2020-08-28 Sodium citrate buffer solution preparation kit Active CN212980732U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021841191.1U CN212980732U (en) 2020-08-28 2020-08-28 Sodium citrate buffer solution preparation kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021841191.1U CN212980732U (en) 2020-08-28 2020-08-28 Sodium citrate buffer solution preparation kit

Publications (1)

Publication Number Publication Date
CN212980732U true CN212980732U (en) 2021-04-16

Family

ID=75435545

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021841191.1U Active CN212980732U (en) 2020-08-28 2020-08-28 Sodium citrate buffer solution preparation kit

Country Status (1)

Country Link
CN (1) CN212980732U (en)

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