CN212942851U - Polyacrylamide gel preparation facilities - Google Patents

Polyacrylamide gel preparation facilities Download PDF

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Publication number
CN212942851U
CN212942851U CN202021350228.0U CN202021350228U CN212942851U CN 212942851 U CN212942851 U CN 212942851U CN 202021350228 U CN202021350228 U CN 202021350228U CN 212942851 U CN212942851 U CN 212942851U
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positioning
strip
polyacrylamide gel
shaped
offset plate
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宋斌斌
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Anhui Haotuo Biotechnology Co ltd
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Anhui Haotuo Biotechnology Co ltd
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Abstract

The utility model provides a polyacrylamide gel preparation facilities, belongs to immunity test experimental device technical field, and this preparation facilities includes the anchor clamps main part, the glass offset plate, sealed pad and locating component, the anchor clamps main part sets up to the sealed box body structure of rectangle, lay sealed the pad in the anchor clamps main part, the both sides of anchor clamps main part are provided with multiunit locating component along its length direction relatively, multiunit locating component is fixed with the both sides location of sealed pad, roll the glass offset plate between adjacent two sets of locating component and compress tightly the location, form the encapsulating space between glass offset plate and the sealed pad, the beneficial effects of the utility model are that, this preparation facilities overall structure is simple, makes the location of glass offset plate more stable, can seal glass offset plate fast, has prevented the problem of leaking glue, has improved the quality and the efficiency of system glue, has realized the large-scale production of polyacrylamide gel.

Description

Polyacrylamide gel preparation facilities
Technical Field
The utility model relates to an immunoassay test experimental device technical field especially relates to a polyacrylamide gel preparation facilities.
Background
Polyacrylamide gel is a material used in the biomedical field for biochemical tests. It can isolate protein or nucleic acid molecules, and is typically used to analyze sample components. The gel mainly comprises an acrylamide solution, a buffer solution, deionized water, a coagulant and a catalyst to form glue solutions with different concentrations, and under the action of the coagulant and the catalyst, the solution forms a space net structure which plays a role of a molecular sieve when separating protein or nucleic acid.
The common method in the current prefabricated glue production process is that the rubber plate is made of plastic materials, and the bottom is sealed by an adhesive tape or agarose gel. The adhesive tape sealing method is widely applied to large-scale production and is a mainstream process in the market at present, but because of the polar factors of the plastic rubber plate material, the adhesive tape sealing method has an adsorption effect on protein, so that the effect of the adhesive tape sealing method is not as good as that of the traditional glass material, the glass rubber plate cannot be sealed by the adhesive tape, the glass rubber plate is sealed by the agarose gel at present, the agarose gel needs to be prepared in advance, the agarose gel is poured into the bottom of the glass rubber plate, the sealing is realized after the agarose gel is cooled, the time used in the whole sealing process is longer, the preparation efficiency of the polyacrylamide gel is low, and the large-scale production cannot be realized. Moreover, the glass rubber plate needs to be manually inserted into the rubber making groove by a person and then is positioned and fixed through structures such as the clamping blocks, the positioning process of the glass rubber plate is troublesome, the glass rubber plate is not stable enough after being positioned, the position of the glass rubber plate cannot be kept consistent, and the quality of rubber making can be influenced.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a simple structure, the location of glass offset plate is convenient, stable, can realize large-scale production's polyacrylamide gel preparation facilities with glass offset plate quick seal moreover.
In order to achieve the above object, the present invention provides a technical solution for solving the technical problem: polyacrylamide gel preparation facilities, including anchor clamps main part, glass offset plate, sealed pad and locating component, the anchor clamps main part sets up to the sealed box body structure of rectangle, lay in the anchor clamps main part sealed pad, the both sides of anchor clamps main part are provided with the multiunit relatively along its length direction locating component, multiunit locating component will sealed both sides location of filling up is fixed, will between two sets of adjacent locating component the glass offset plate rolls and compresses tightly the location, form the encapsulating space between glass offset plate and the sealed pad.
Furthermore, the positioning assembly comprises a positioning seat, a pin cylinder and a positioning clamping strip, the positioning seat is fixed on the inner wall of the clamp main body, the positioning seat is internally connected with the pin cylinder in a vertical sliding mode, a positioning groove is formed between the positioning seat and the upper end of the pin cylinder at a certain distance, and the positioning clamping strip is inserted into the positioning groove from the outer side of the clamp main body to block and position the pin cylinder.
Furthermore, the positioning seat is set to be a U-shaped seat, a cross-shaped guide groove is formed in the positioning seat, strip-shaped positioning holes I are oppositely formed in two sides of the upper portion of the positioning seat, the cross-shaped guide groove is in up-and-down sliding fit with the pin column body, the strip-shaped positioning holes I are in splicing fit with the positioning clamping strips, and the upper end of the pin column body is parallel and level to the bottom end of the strip-shaped positioning holes I.
Further, the round pin post body includes ten fonts and one-way bearing, be provided with the length direction vertically mounting hole with it in the cross, the both ends of mounting hole rotate respectively and are connected with a plurality of one-way bearings, the rotation direction of the one-way bearing at mounting hole both ends is opposite and all rotates towards the outside of cross, between two adjacent one-way bearings with glass offset roll contact.
Further, the positioning clamp strip comprises a fixing plate and positioning teeth, a plurality of strip-shaped positioning holes II are formed in the upper ends of the two sides of the clamp body at intervals along the length direction of the clamp body, a plurality of positioning teeth are formed in the fixing plate at intervals along the length direction of the fixing plate, and the positioning teeth penetrate through the strip-shaped positioning holes II and then are connected into the two strip-shaped positioning holes I in a penetrating mode.
Further, the longitudinal section of the end of the positioning tooth is triangular.
Further, the two ends of the clamp main body are provided with strip-shaped openings convenient to hold.
Further, the sealing gasket is arranged to be a corrosion-resistant rubber gasket or a PVC cushion.
The utility model has the advantages that:
1. the utility model discloses a place sealed the pad in the anchor clamps main part to both sides in sealed pad top set up multiunit locating component relatively, roll through adjacent two sets of locating component with the glass offset plate and compress tightly the location, prevented the pine of glass offset plate and taken off, make the location of glass offset plate more stable, make glass offset plate and sealed fill up between form sealed encapsulating space, it is more swift to make sealed, prevented the problem of leaking to glue, the quality of system is glued has been improved, applicable in the mass production use of polyacrylamide gel.
2. The positioning assembly comprises a positioning seat, a pin cylinder body and positioning clamping strips, the positioning seat is fixed on the inner wall of the clamp main body, the pin cylinder body is installed in the positioning seat, the position of the pin cylinder body is fixed through the positioning clamping strips, two opposite sides of the pin cylinder body are rotatably connected with a plurality of one-way bearings which are all rotated towards the outer side, when a glass rubber plate is inserted between two adjacent groups of positioning assemblies, the glass rubber plate is in rolling contact with the one-way bearings, the glass rubber plate is inserted more quickly, the bottom end of the glass rubber plate is abutted against a sealing gasket to complete sealing positioning, the reverse ejection of the glass rubber plate is prevented, the positioning of the glass rubber plate is more convenient and quicker, the integral structure is more stable after positioning, after the rubber manufacturing is completed, the positioning clamping strips are pulled out to loosen the pin cylinder body, the next production is convenient after the glass rubber plate is taken out, and the taking-out of the glass rubber plate is more, the efficiency of system glue is improved.
To sum up, this preparation facilities overall structure is simple, makes the location of glass offset plate more stable, can seal the glass offset plate fast, has prevented the problem of leaking to glue, has improved the quality and the efficiency of system glue, has realized the large-scale production of polyacrylamide gel.
Drawings
The contents of the various figures of the specification and the labels in the figures are briefly described as follows:
FIG. 1 is a schematic view of the assembled structure of the present invention;
FIG. 2 is an exploded view of FIG. 1;
FIG. 3 is a schematic structural view of the positioning assembly of FIG. 1;
FIG. 4 is an exploded view of FIG. 3;
the labels in the above figures are: 1. the fixture comprises a fixture main body, 11 bar-shaped positioning holes II, 12 bar-shaped openings, 2 glass rubber plates, 3 sealing gaskets, 4 positioning components, 41 positioning seats, 411 cross-shaped guide grooves, 412 bar-shaped positioning holes I, 42 pin cylinders, 421 cross characters, 422 unidirectional bearings, 423 mounting holes, 43 positioning clamping strips, 431 fixing plates, 432 positioning teeth, 44 positioning grooves and 5 glue filling spaces.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments, and the following embodiments are used for illustrating the present invention, but do not limit the scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The utility model discloses specific embodiment does: as shown in figures 1 and 2, a polyacrylamide gel preparation device comprises a clamp main body 1, a glass rubber plate 2, a sealing gasket 3 and positioning components 4, wherein the clamp main body 1 is arranged into a rectangular sealing box structure, the sealing gasket 3 is laid in the clamp main body 1, a plurality of groups of positioning components 4 are oppositely arranged on two sides of the clamp main body 1 along the length direction of the clamp main body, the two sides of the sealing gasket 3 are positioned and fixed by the groups of positioning components 4, the glass rubber plate 2 is rolled, pressed and positioned between two adjacent groups of positioning components 4, the glass rubber plate 2 is prevented from loosening, the positioning of the glass rubber plate is more stable, the glass rubber plate 2 is a glass rubber box with openings at the upper end and the lower end, and a glue filling space 5 is formed between the glass rubber plate 2 and the sealing gasket 3, so that sealing is quicker, the problem of glue leakage is solved, the glue making quality is improved, and the glass rubber plate is suitable for mass production and use of polyacrylamide gel.
Specifically, as shown in fig. 3 and 4, the positioning assembly 4 includes a positioning seat 41, a pin cylinder 42 and a positioning clamping strip 43, the positioning seat 41 is fixed on the inner wall of the fixture body 1 by welding or screwing, so that the positioning seat 41 is pressed above the sealing gasket 3, on one hand, the sealing gasket 3 is positioned and fixed, on the other hand, the pin cylinder 42 is connected in the positioning seat 41 in a sliding manner, the pin cylinder 42 contacts with the glass cement plate 2, and the fixing of the position of the positioning seat 41 determines the determination of the insertion position of the glass cement plate 2. The positioning seat 41 and the upper end of the pin cylinder 42 are separated by a distance to form a positioning groove 44, and the positioning clip strip 43 is inserted into the positioning groove 44 from the outer side of the clamp body 1 to stop and position the pin cylinder 42, so that the vertical position of the pin cylinder 42 is fixed, and the influence of the loosening of the pin cylinder 42 on glue making is prevented.
Specifically, above-mentioned positioning seat 41 sets up to the U-shaped seat, be provided with cross guide way 411 in the positioning seat 41, the upper portion both sides of positioning seat 41 are provided with bar locating hole I412 relatively, cross guide way 411 and round pin post 42 are sliding fit from top to bottom, the rocking of round pin post in the circumferential direction has been prevented, bar locating hole I412 is pegged graft with locator card strip 43 and is cooperated, the upper end of round pin post 42 is leveled mutually with the bottom of bar locating hole I412, after inserting locator card strip 43 in bar locating hole I412, the bottom plane of locator card strip 43 contacts with the upper end of round pin post 42, the influence that round pin post 42 caused at the not hard up system of vertical direction to glue has been prevented. Therefore, the positioning seat 41 and the positioning clamping strip 43 limit the degree of freedom of the pin cylinder 42 in six directions, so that the pin cylinder 42 is positioned more stably and firmly.
Specifically, the pin cylinder 42 includes a cross-shaped body 421 and a one-way bearing 422, a mounting hole 423 perpendicular to the length direction is provided in the cross-shaped body 421, two ends of the mounting hole 423 are respectively connected with a plurality of one-way bearings 422 in a rotating manner, the one-way bearings 422 at two ends of the mounting hole 423 rotate in opposite directions and rotate towards the outer side of the cross-shaped body 421, when the glass rubber plate 2 is inserted between two adjacent one-way bearings 422, the glass rubber plate 2 is in rolling contact with the one-way bearing 422, so that the glass rubber plate 2 is inserted more quickly, and when the bottom end of the glass rubber plate 2 abuts against the sealing gasket 3 to complete sealing and positioning, because the one-way bearing 422 can only rotate in one direction, the glass rubber plate 2 is rolled, pressed and positioned, so that the glass rubber plate 2 is prevented from reversely popping up, the positioning of the glass rubber plate 2 is more convenient and faster, the whole structure after positioning is more stable, and after the, through extracting the locator card strip 43, make round pin post body 42 not hard up, be convenient for take out the back with glass offset plate 2 to carrying out production on next step, make taking out of glass offset plate more convenient and fast, improved the efficiency of system glue.
Specifically, the positioning clamping strip 43 comprises a fixing plate 431 and positioning teeth 432, a plurality of strip-shaped positioning holes II 11 are formed in the upper ends of the two sides of the clamp body 1 at intervals along the length direction of the clamp body, a plurality of positioning teeth 432 are formed in the fixing plate 431 at intervals along the length direction of the fixing plate, and the positioning teeth 432 penetrate through the strip-shaped positioning holes II 11 and then are connected into the two strip-shaped positioning holes I412 in a penetrating mode, so that the integral positioning of a plurality of pin columns 42 on one side of the clamp body 1 is realized, and the positioning is quicker. In addition, in order to make it easier for the positioning teeth 432 to be inserted into the bar-shaped positioning holes i 412 and ii 11, the longitudinal section of the end of the positioning teeth 432 is provided in a triangular shape.
In addition, as shown in fig. 1 and 2, the two ends of the clamp main body 1 are provided with strip-shaped openings 12 which are convenient to hold, so that the clamp main body 1 can be conveniently and manually held and moved, and the operation is more flexible; the sealing gasket 3 is a corrosion-resistant rubber gasket or a PVC cushion, and is soft, corrosion-resistant and low in replacement cost.
The assembling method of the preparation device comprises the following steps: firstly, placing a sealing gasket at the bottom of a clamp main body to ensure that the sealing gasket is fully paved on the bottom plane of the clamp main body, then oppositely placing positioning seats 41 at equal intervals on two sides of the clamp main body 1, and fixing a plurality of groups of opposite positioning seats 41 on the inner walls of the two sides of the clamp main body 1 in a bolt connecting piece or welding mode; then, ensuring that the pin cylinder 42 is installed in the cross-shaped guide groove 411 of each positioning seat 41, ensuring that the rotation directions of the one-way bearings 422 at the two ends of the installation hole 423 on the pin cylinder 42 are opposite and all face outwards, manually holding the fixing plate 431 of the positioning clamping strip 43, enabling the positioning teeth 432 on the fixing plate 431 to be opposite to the strip-shaped positioning holes II 11 at one side of the clamp body, inserting the positioning teeth 432 into the strip-shaped positioning holes II 11, enabling the positioning teeth 432 to penetrate through the two strip-shaped positioning holes I412 after penetrating through the strip-shaped positioning holes II 11, and limiting and fixing the upper end of the pin cylinder 42; finally, insert a plurality of glass offset plates 2 through manipulator or manual mode in adjacent two sets of locating component 4, make glass offset plate 2 insert rapidly under one-way rolling effect of one-way bearing 422, glass offset plate 2 can't be extracted, accomplish sealed location after the bottom of glass offset plate 2 offsets with sealed pad 3, then accomplished the equipment of whole device.
After the device is assembled, the prepared polyacrylamide gel solution is poured into the glue pouring space 5 in the middle of the glass rubber plate 2, the glue pouring process is completed at one time, bubbles are prevented from being generated, the sample comb is stably inserted at one time after the glue pouring is completed so as to form a sample dispensing hole, and the final glue preparation production is completed. After the glue is made, the positioning clamping strip 43 is pulled out, the pin column body 42 is loosened, the glass glue plate 2 is taken out conveniently, the glass glue plate 2 is sent to the next procedure, and the steps are repeated to carry out the next round of production.
To sum up, this polyacrylamide gel preparation facilities overall structure is simple, makes the location of glass offset plate more stable, can seal glass offset plate fast, has prevented the problem of leaking to glue, has improved the quality and the efficiency of system glue, has realized the large-scale production of polyacrylamide gel.
The foregoing is merely illustrative of some of the principles of the present invention and the description is not intended to limit the invention to the specific constructions and applications shown, so that all modifications and equivalents that may be utilized are within the scope of the invention.

Claims (8)

1. The utility model provides a polyacrylamide gel preparation facilities, its characterized in that, includes anchor clamps main part (1), glass offset plate (2), sealed pad (3) and locating component (4), anchor clamps main part (1) sets up to the sealed box body structure of rectangle, lay in anchor clamps main part (1) sealed pad (3), the both sides of anchor clamps main part (1) are provided with the multiunit relatively along its length direction locating component (4), multiunit locating component (4) will the both sides location of sealed pad (3) is fixed, will between two sets of adjacent locating component (4) glass offset plate (2) roll and compress tightly the location, form encapsulating space (5) between glass offset plate (2) and the sealed pad (3).
2. The apparatus for preparing polyacrylamide gel according to claim 1, wherein: the positioning assembly (4) comprises a positioning seat (41), a pin column body (42) and a positioning clamping strip (43), the positioning seat (41) is fixed on the inner wall of the clamp main body (1), the positioning seat (41) is connected with the pin column body (42) in a vertical sliding mode, a positioning groove (44) is formed between the positioning seat (41) and the upper end of the pin column body (42) at a certain distance, and the positioning clamping strip (43) is inserted into the positioning groove (44) from the outer side of the clamp main body (1) to block and position the pin column body (42).
3. The apparatus for preparing polyacrylamide gel according to claim 2, wherein: the positioning seat (41) is set to be a U-shaped seat, a cross-shaped guide groove (411) is arranged in the positioning seat (41), strip-shaped positioning holes I (412) are oppositely arranged on two sides of the upper portion of the positioning seat (41), the cross-shaped guide groove (411) is in up-and-down sliding fit with the pin column body (42), the strip-shaped positioning holes I (412) are in plug fit with the positioning clamping strips (43), and the upper end of the pin column body (42) is flush with the bottom end of the strip-shaped positioning holes I (412).
4. The apparatus for preparing polyacrylamide gel according to claim 3, wherein: the round pin cylinder (42) is including ten fonts (421) and one-way bearing (422), be provided with in ten fonts (421) with its length direction vertically mounting hole (423), the both ends of mounting hole (423) are rotated respectively and are connected with a plurality of one-way bearing (422), the rotation direction of one-way bearing (422) at mounting hole (423) both ends is opposite and all rotates towards the outside of cross (421), between two adjacent one-way bearing (422) with glass offset plate (2) rolling contact.
5. The apparatus for preparing polyacrylamide gel according to claim 3, wherein: the positioning clamping strip (43) comprises a fixing plate (431) and positioning teeth (432), a plurality of strip-shaped positioning holes II (11) are formed in the upper ends of the two sides of the clamp body (1) at intervals along the length direction of the clamp body, a plurality of positioning teeth (432) are formed in the fixing plate (431) at intervals along the length direction of the fixing plate, and the positioning teeth (432) penetrate through the strip-shaped positioning holes II (11) and then are connected in the two strip-shaped positioning holes I (412).
6. The apparatus for preparing polyacrylamide gel according to claim 5, wherein: the longitudinal section of the end part of the positioning tooth (432) is triangular.
7. The apparatus for preparing polyacrylamide gel according to any one of claims 1 to 6, wherein: the two ends of the clamp main body (1) are provided with strip-shaped openings (12) which are convenient to hold.
8. The apparatus for preparing polyacrylamide gel according to claim 7, wherein: the sealing gasket (3) is a corrosion-resistant rubber gasket or a PVC cushion.
CN202021350228.0U 2020-07-10 2020-07-10 Polyacrylamide gel preparation facilities Active CN212942851U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021350228.0U CN212942851U (en) 2020-07-10 2020-07-10 Polyacrylamide gel preparation facilities

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021350228.0U CN212942851U (en) 2020-07-10 2020-07-10 Polyacrylamide gel preparation facilities

Publications (1)

Publication Number Publication Date
CN212942851U true CN212942851U (en) 2021-04-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202021350228.0U Active CN212942851U (en) 2020-07-10 2020-07-10 Polyacrylamide gel preparation facilities

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CN (1) CN212942851U (en)

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