CN212924996U

CN212924996U - Full-automatic nucleic acid extraction instrument with improved extraction precision

Info

Publication number: CN212924996U
Application number: CN202021629863.2U
Authority: CN
Inventors: 蒋自远
Original assignee: Xiangyang Yuanjie Precision Machinery Co Ltd
Current assignee: Xiangyang Yuanjie Precision Machinery Co Ltd
Priority date: 2020-08-07
Filing date: 2020-08-07
Publication date: 2021-04-09
Anticipated expiration: 2030-08-07

Abstract

The utility model discloses a full-automatic nucleic acid extractor with improved extraction precision, relating to the technical field of nucleic acid extraction, comprising a machine body, wherein the front side of the machine body is provided with a purification cavity and the side surface of the machine body is provided with an air inlet and air filtering mechanism; the air inlet is strained gas and is constructed including inlaying the board, it sets up in the organism side to inlay the fixed nestification of board, it is provided with the intake pipe to inlay the fixed intake pipe that runs through in board side top, the intake pipe runs through and inlays the board and extend to inside the purification cavity, intake pipe tip fixedly connected with sealed cowling, the inside fixed air pump and the sealed cowling tip that is provided with of sealed cowling bond and be provided with the casing, the casing is fixed to be set up in organism side top, the inside packing of casing has active carbon particle and casing top and the bottom all to fix the nestification and be provided with. The utility model discloses can be more effectual with the inside extraction reagent after volatilizing of purification cavity discharge completely, avoid remaining, and further improve the extraction precision of nucleic acid sample.

Description

Full-automatic nucleic acid extraction instrument with improved extraction precision

Technical Field

The utility model relates to a nucleic acid extraction technical field, in particular to full-automatic nucleic acid extraction appearance that extraction precision improves.

Background

With the rapid development of molecular biology, the importance of nucleic acid-based molecular diagnosis and detection technology is gradually highlighted, nucleic acid is derived from purification and extraction of biological samples, and when nucleic acid is purified and extracted, a nucleic acid extractor is required to be used.

However, the above-mentioned nucleic acid extracting apparatus still has some disadvantages in practical use, such as that the chamber for extracting the nucleic acid sample has good sealing performance during the nucleic acid extracting process, and the fan needs to assist the airflow during the process of exhausting the volatilized extracting reagent, which results in that when the air in the extracting chamber is exhausted to a great extent, there are still more volatilized extracting reagent which is not exhausted, and the practical use effect is not ideal.

Therefore, it is necessary to provide a fully automatic nucleic acid extracting apparatus with improved extraction accuracy to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a draw full-automatic nucleic acid extraction appearance that precision improves to solve and to provide in the above-mentioned background art because its cavity that is used for drawing the nucleic acid sample has good leakproofness in the nucleic acid extraction in-process, and the fan will volatilize the back and draw reagent exhaust in-process and need be with the help of the air current, just so lead to when drawing the cavity interior air and being got rid of most, still have the more condition of not being discharged of extracting reagent after volatilizing, the not ideal problem of actual use effect.

In order to achieve the above object, the utility model provides a following technical scheme: a full-automatic nucleic acid extraction instrument with improved extraction precision comprises an instrument body, wherein the front surface of the instrument body is provided with a purification chamber, and the side surface of the instrument body is provided with an air inlet and air filtering mechanism;

the air inlet is strained gas and is constructed including inlaying the board, it sets up in the organism side to inlay the fixed nestification of board, it is provided with the intake pipe to inlay the fixed intake pipe that runs through in board side top, the intake pipe runs through and inlays the board and extend to inside the purification cavity, intake pipe tip fixedly connected with sealed cowling, the inside fixed air pump and the sealed cowling tip that is provided with of sealed cowling bond and be provided with the casing, the casing is fixed to be set up in organism side top, the inside packing of casing has active carbon particle and casing top and the bottom all to fix the nestification and be provided with.

Preferably, the bottom of the side surface of the embedded plate is fixedly provided with an exhaust pipe in a penetrating manner, and the exhaust pipe penetrates through the embedded plate and extends into the purification chamber.

Preferably, the end of the exhaust pipe is connected with a water bath tank, the water bath tank is fixedly arranged at the bottom of the side face of the machine body, and the bottom end of the exhaust pipe penetrates through the water bath tank and extends to the bottom of the inner cavity of the water bath tank.

Preferably, one side of the top of the water bath box is fixedly provided with an air outlet pipeline in a penetrating mode, the bottom of the side face of the water bath box is fixedly provided with a drain pipe in a penetrating mode, and the end portion of the drain pipe is in threaded connection with a sealing plug.

Preferably, the bearing board has been placed to purification cavity inside, be provided with the limiting plate under the bearing board, the equal fixed connecting rod that is provided with in limiting plate top four corners, connecting rod top and bearing board fixed connection.

Preferably, one side of the front surface of the machine body is movably connected with a sealing door through a hinge, and a glass observation window is fixedly nested in the middle of the front surface of the sealing door.

The utility model discloses a technological effect and advantage:

1. the utility model is provided with an air inlet pipe, a sealing cover, an air pump, a shell, activated carbon particles and a filter screen, so that when the air pump generates negative pressure near the air inlet end of the shell, the outside air sequentially passes through the filter screen at the top of the shell, the activated carbon particles inside the shell and the filter screen at the bottom of the shell, then is input into the air inlet pipe through the air outlet end of the air pump, and finally is blown into the purification chamber from the inside of the air inlet pipe, thereby driving the extraction reagent volatilized inside the purification chamber to enter the exhaust pipe, further continuously discharging the extraction reagent volatilized inside the purification chamber to the outside of the purification chamber, compared with the same type device in the prior art, the utility model can more effectively discharge the extraction reagent volatilized inside the purification chamber completely without depending on the air inside the purification chamber when discharging the extraction reagent volatilized, the residue is avoided, and the extraction precision of the nucleic acid sample is further improved;

2. the utility model discloses a be provided with blast pipe, water bath and the pipeline of giving vent to anger to in the aquatic of water bath inside can be entered into through the blast pipe to the reagent of drawing after volatilizing, the reagent of drawing after volatilizing is dissolved in water, and the air then becomes the bubble and emerges the surface of water and break, enters into the atmosphere by the pipeline of giving vent to anger at last, and then avoids drawing after volatilizing in the reagent directly enters into the room air and causes harmful effects or produces pungent smell to the human body.

Drawings

Fig. 1 is a schematic view of the overall front view structure of the present invention.

Fig. 2 is the schematic view of the sectional structure of the upper part of the air intake and filtering mechanism of the present invention.

Fig. 3 is the schematic view of the cross-sectional structure of the lower part of the air intake and filtering mechanism of the present invention.

In the figure: 1. a body; 2. a purification chamber; 3. an air inlet and filtering mechanism; 4. a built-in plate; 5. an air inlet pipe; 6. a sealing cover; 7. an air pump; 8. a housing; 9. activated carbon particles; 10. a filter screen; 11. an exhaust pipe; 12. a water bath tank; 13. an air outlet pipe; 14. a drain pipe; 15. a sealing plug; 16. a carrier plate; 17. a limiting plate; 18. a connecting rod; 19. a sealing door; 20. a glass viewing window.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model provides a full-automatic nucleic acid extraction appearance that draws precision to improve as shown in fig. 1-3, as shown in fig. 1, including organism 1, organism 1 openly has seted up purification cavity 2 and organism 1 side sets up air inlet and strains gas mechanism 3.

More specifically, purification cavity 2 is inside to be placed loading board 16, sets up limiting plate 17 under loading board 16, and limiting plate 17 top four corners is all fixed and is set up connecting rod 18, 18 tops of connecting rod and 16 fixed connection of loading board to utilize loading board 16 to place the test tube that has biological sample in the splendid attire, utilize limiting plate 17 to carry on spacingly to the test tube bottom simultaneously.

It should be noted that, a sealing door 19 is movably connected to one side of the front surface of the machine body 1 through a hinge, and a glass observation window 20 is fixedly nested in the middle of the front surface of the sealing door 19, so that a user can observe the extraction condition of the nucleic acid sample inside the purification chamber 2 through the glass observation window 20.

As shown in fig. 2, the air intake and filtering mechanism 3 includes an embedded plate 4, the embedded plate 4 is fixedly embedded in the side of the machine body 1, an air intake pipe 5 is fixedly arranged at the top of the side of the embedded plate 4, the air intake pipe 5 penetrates the embedded plate 4 and extends into the purification chamber 2, the end of the air intake pipe 5 is fixedly connected with a sealing cover 6, an air pump 7 is fixedly arranged in the sealing cover 6, a housing 8 is fixedly arranged at the end of the sealing cover 6, the housing 8 is fixedly arranged at the top of the side of the machine body 1, activated carbon particles 9 are filled in the housing 8, and filter screens 10 are fixedly embedded at the top and the bottom of the housing 8, so that when the air pump 7 generates negative pressure near the air intake end of the housing 8, the external air sequentially passes through the filter screen 10 at the top of the housing 8, the activated carbon particles 9 in the housing 8, and the filter screen 10 at the bottom, at last, the inside of the purification chamber 2 is blown in from the inside of the air inlet pipe 5, so that the extraction reagent volatilized from the inside of the purification chamber 2 is driven to enter the exhaust pipe 11, and the extraction reagent volatilized from the inside of the purification chamber 2 is continuously discharged to the outside of the purification chamber 2.

As shown in fig. 3, inlay 4 fixed the running through in board side bottom and set up blast pipe 11, inside blast pipe 11 runs through and inlays board 4 and extend to

purification cavity

2, 11 end connection of blast pipe has water bath 12, water bath 12 is fixed to be set up in 1 side bottom of organism, 11 bottoms of blast pipe run through water bath 12 and extend to 12 inner chamber bottoms of water bath, 12 top one side of water bath are fixed to run through and set up outlet duct 13, so that the extraction reagent after volatilizing can enter into the inside aquatic of water bath 12 through blast pipe 11, the extraction reagent after volatilizing is dissolved in water, the air then becomes the bubble and floats out the surface of water and breaks, enter into the atmosphere by outlet duct 13 at last.

In addition, the drain pipe 14 is fixedly arranged at the bottom of the side face of the water bath box 12 in a penetrating mode, the end part of the drain pipe 14 is in threaded connection with the sealing plug 15, so that when water in the water bath box 12 needs to be replaced, a user can unscrew the sealing plug 15 at the end part of the drain pipe 14, at the moment, the water in the water bath box 12 is discharged through the drain pipe 14, after the water is completely discharged, the sealing plug 15 is reset, and then water is added into the water bath box 12 through the air outlet pipeline 13.

The utility model discloses the theory of operation:

during actual use, after the air pump 7 is started, negative pressure is generated at the air inlet end, close to the shell 8, of the air pump 7, external air sequentially passes through the filter screen 10 at the top of the shell 8, the activated carbon particles 9 in the shell 8 and the filter screen 10 at the bottom of the shell 8 and is sucked by the air pump 7, then the external air is input into the air inlet pipe 5 through the exhaust end of the air pump 7, and finally the external air is blown into the purification chamber 2 from the inside of the air inlet pipe 5, so that the extraction reagent volatilized from the inside of the purification chamber 2 is driven to enter the exhaust pipe 11 and then enters the water in the water bath box 12 through the exhaust pipe 11, the volatilized extraction reagent is dissolved in the water, the air becomes bubbles to float out of the water surface and break;

when the water in the water bath box 12 needs to be replaced, a user can screw down the sealing plug 15 at the end of the drain pipe 14, at the moment, the water in the water bath box 12 is discharged through the drain pipe 14, after the water is completely discharged, the sealing plug 15 is reset, and then the water is added into the water bath box 12 through the air outlet pipeline 13.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. The utility model provides a full-automatic nucleic acid extraction appearance that extraction precision improves which characterized in that: comprises a machine body (1), wherein the front surface of the machine body (1) is provided with a purification chamber (2), and the side surface of the machine body (1) is provided with an air inlet and air filtering mechanism (3);

air inlet gas filtering mechanism (3) is including embedding board (4), it sets up in organism (1) side to embed board (4) fixed nestification, it is provided with intake pipe (5) to embed fixed running through in board (4) side top, intake pipe (5) run through and embed board (4) and extend to purification cavity (2) inside, intake pipe (5) tip fixedly connected with seal cover (6), seal cover (6) inside fixed air pump (7) and seal cover (6) tip bonding are provided with casing (8), casing (8) are fixed to be set up in organism (1) side top, casing (8) inside packing has activated carbon granule (9) and casing (8) top and bottom all to fix nestification and be provided with filter screen (10).

2. The full-automatic nucleic acid extractor with improved extraction accuracy according to claim 1, wherein: the bottom of the side face of the embedded plate (4) is fixedly provided with an exhaust pipe (11) in a penetrating mode, and the exhaust pipe (11) penetrates through the embedded plate (4) and extends into the purification chamber (2).

3. The full-automatic nucleic acid extractor with improved extraction accuracy according to claim 2, wherein: blast pipe (11) end connection has water bath (12), water bath (12) are fixed to be set up in organism (1) side bottom, blast pipe (11) bottom runs through water bath (12) and extends to water bath (12) inner chamber bottom.

4. The full-automatic nucleic acid extractor with improved extraction accuracy according to claim 3, wherein: the air outlet pipeline (13) is fixedly arranged on one side of the top of the water bath tank (12) in a penetrating mode, the drain pipe (14) is fixedly arranged at the bottom of the side face of the water bath tank (12) in a penetrating mode, and the end portion of the drain pipe (14) is connected with the sealing plug (15) in a threaded mode.

5. The full-automatic nucleic acid extractor with improved extraction accuracy according to claim 1, wherein: purification cavity (2) inside has placed loading board (16), be provided with limiting plate (17) under loading board (16), limiting plate (17) top four corners is all fixed and is provided with connecting rod (18), connecting rod (18) top and loading board (16) fixed connection.

6. The full-automatic nucleic acid extractor with improved extraction accuracy according to claim 1, wherein: one side of the front surface of the machine body (1) is movably connected with a sealing door (19) through a hinge, and a glass observation window (20) is fixedly nested in the middle of the front surface of the sealing door (19).