CN212800360U

CN212800360U - Micro-fluidic chip for virus detection

Info

Publication number: CN212800360U
Application number: CN202020804030.9U
Authority: CN
Inventors: 郭金城; 刘岩
Original assignee: Suzhou Baidao Medical Technology Co ltd
Current assignee: Suzhou Baidao Medical Technology Co ltd
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2021-03-26
Anticipated expiration: 2030-05-14

Abstract

The utility model relates to a micro-fluidic chip for virus detection, it includes the last chip of superpose and chip down, goes up the chip and connects with chip bonding down, guarantees to go up the chip and totally closed the connection between the chip down. A plurality of first detection grooves are formed in the top surface of the lower chip, a plurality of second detection grooves are formed in the bottom surface of the upper chip, a plurality of first flow channels, a reaction cavity, a second flow channel and a waste liquid cavity which are communicated in sequence are formed after the upper chip and the lower chip are butted, and a plurality of liquid injection holes and a plurality of air holes are formed in the upper chip. The scheme integrates the steps of sample introduction, mixing, reaction and the like into a chip, and the method is a totally-enclosed flow, so that the virus is prevented from diffusing into the air to pollute the environment. In the whole virus detection process, the sample liquid, the detection reagent and the waste liquid are all sealed and retained in the chip, so that the pollution of biological waste is greatly reduced. The liquid inlet, reaction, detection and other flows of the multi-path samples can be carried out simultaneously, the flow channels are independent from each other, cross influence is avoided, and the detection efficiency is effectively improved.

Description

Micro-fluidic chip for virus detection

Technical Field

The utility model relates to a virus detection technology field especially relates to a micro-fluidic chip for virus detection.

Background

Respiratory infections are one of the most common diseases, especially in the pediatric population. The incidence of respiratory infections is extremely high, and is divided into upper respiratory infections and lower respiratory infections, and the upper respiratory infections are basically viral infections and very few bacterial infections. At present, most of medical institutions do not detect viruses of respiratory tract infection, but directly use antibiotics for treatment, and the antibiotics cannot treat the respiratory tract infection caused by the viruses, so that abuse of the antibiotics is caused, and meanwhile, the drug resistance of related bacteria is enhanced. Although lower respiratory tract infections occur less frequently, these infections are more harmful to humans. Meanwhile, it is noted that RSV, bacteria, adenovirus and influenza virus are all likely to be infectious pathogens to doctors, and therefore, respiratory virus detection is very necessary. The existing detection equipment has a complex structure, needs a large amount of manual operation, is easy to cause environmental pollution in the operation process, and seriously influences the virus detection efficiency.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

In view of the above disadvantages and deficiencies of the prior art, the utility model provides a micro-fluidic chip for virus detection, which solves the technical problems of low detection efficiency and easy environmental pollution of the prior devices.

(II) technical scheme

In order to achieve the above object, the utility model discloses a main technical scheme include:

a microfluidic chip for virus detection comprises an upper chip and a lower chip which are stacked and are connected in a bonding manner;

the top surface of the lower chip is provided with a plurality of first detection grooves, and the first detection grooves comprise a first microflow groove, a first microflow pool, a second microflow groove and a second microflow pool which are sequentially communicated;

the bottom surface of the upper chip is provided with a plurality of second detection grooves, and the second detection grooves comprise a third microflow groove, a third microflow pool, a fourth microflow groove and a fourth microflow pool which are sequentially communicated;

the first microflow groove and the third microflow groove are butted to form a first flow channel, the first microflow pool and the third microflow pool are butted to form a reaction cavity, the second microflow groove and the fourth microflow groove are butted to form a second flow channel, and the second microflow pool and the fourth microflow pool are butted to form a waste liquid cavity;

the upper chip is provided with a plurality of liquid injection holes and a plurality of air holes, the liquid injection holes are connected with the first microflow grooves in a one-to-one correspondence manner, and the air holes are connected with the fourth microflow grooves in a one-to-one correspondence manner.

Preferably, the first flow passage and the second flow passage are both cylindrical passages.

Preferably, the groove bottoms of the first and second micro flow grooves are at the same level.

Preferably, the horizontal height of the bottom of the first microflow groove is greater than that of the bottom of the first microflow groove;

the horizontal height of the bottom of the first micro-flow pool is greater than that of the bottom of the second micro-flow pool.

Preferably, the bottom of the third microflow cell, the bottom of the fourth microflow cell, the bottom of the third microflow cell and the bottom of the fourth microflow cell are all at the same level.

Preferably, the liquid injection hole is a gradual change hole or a threaded hole, and the diameter of the gradual change hole is gradually reduced from the upper surface to the lower surface of the upper chip.

Preferably, a plurality of the first detection grooves are distributed at intervals, and a plurality of the second detection grooves are distributed at intervals.

(III) advantageous effects

The utility model has the advantages that: the microfluidic chip comprises an upper chip and a lower chip which are superposed, the upper chip and the lower chip are connected in a bonding manner, the upper chip and the lower chip are ensured to be connected in a totally-enclosed manner, and the connection process is simple. A plurality of first detection grooves are formed in the top surface of the lower chip, a plurality of second detection grooves are formed in the bottom surface of the upper chip, a plurality of first flow channels, a reaction cavity, a second flow channel and a waste liquid cavity which are communicated in sequence are formed after the upper chip and the lower chip are butted, and a plurality of liquid injection holes and a plurality of air holes are formed in the upper chip. The technical scheme is that the steps of sample introduction, mixing, reaction and the like are integrated on a chip, and the whole closed process is adopted, so that the environment pollution caused by the diffusion of viruses into the air is avoided. In the whole virus detection process, the sample liquid, the detection reagent and the waste liquid are all sealed and retained in the chip, so that the pollution of biological waste is greatly reduced. The multiple flow channels in the micro-fluidic chip can realize simultaneous liquid inlet, reaction, detection and other flows of multiple paths of samples, the flow channels are independent from each other, cross influence is avoided, and the detection efficiency is effectively improved.

Drawings

Fig. 1 is a schematic diagram of the whole structure of the microfluidic chip for virus detection according to the present invention;

fig. 2 is a schematic structural diagram of a lower chip of the microfluidic chip for virus detection according to the present invention;

fig. 3 is a schematic structural diagram of an upper chip of the microfluidic chip for virus detection according to the present invention;

fig. 4 is a schematic top view of the microfluidic chip for virus detection according to the present invention;

fig. 5 is a schematic side view of the microfluidic chip for virus detection according to the present invention.

[ description of reference ]

1: chip unloading; 10: a first detection tank; 11: a first microflow channel; 12: a second microflow channel; 13: a first microfluidic cell; 14: a second microfluidic cell;

2: mounting a chip; 20: a second detection tank; 21: a third micro flow groove; 22: a fourth micro flow groove; 23: a third microfluidic cell; 24: a fourth microfluidic cell;

3: a liquid injection hole; 4: a first flow passage; 5: a second flow passage; 6: a reaction chamber; 7: a waste fluid chamber; 8: and (4) air holes.

Detailed Description

For a better understanding of the present invention, reference will now be made in detail to the present invention, examples of which are illustrated in the accompanying drawings. Where directional terms such as "upper", "lower", etc. are used herein, reference is made to the orientation of fig. 5.

The embodiment of the utility model provides a micro-fluidic chip for virus detection has solved current check out test set detection efficiency low, easily causes environmental pollution's technical problem. The microfluidic chip adopts the upper chip 2 and the lower chip 1 which are superposed, and the upper chip 2 and the lower chip 1 are connected in a bonding way, so that the totally-enclosed connection between the upper chip 2 and the lower chip 1 is ensured, and the connection process is simple. A plurality of first detection grooves 10 are formed in the top surface of the lower chip 1, a plurality of second detection grooves 20 are formed in the bottom surface of the upper chip 2, the upper chip 2 and the lower chip 1 are in butt joint to form a plurality of first flow channels 4, a reaction cavity 6, a second flow channel 5 and a waste liquid cavity 7 which are communicated in sequence, and a plurality of liquid injection holes 3 and a plurality of air holes 8 are formed in the upper chip 2. The technical scheme is that the steps of sample introduction, mixing, reaction and the like are integrated on a chip, and the whole closed process is adopted, so that the environment pollution caused by the diffusion of viruses into the air is avoided. In the whole virus detection process, the sample liquid, the detection reagent and the waste liquid are all sealed and retained in the chip, so that the pollution of biological waste is greatly reduced. The multiple flow channels in the micro-fluidic chip can realize simultaneous liquid inlet, reaction, detection and other flows of multiple paths of samples, the flow channels are independent from each other, cross influence is avoided, and the detection efficiency is effectively improved.

In order to better understand the above technical solutions, exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the invention are shown in the drawings, it should be understood that the invention can be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

As shown in fig. 1, the microfluidic chip includes an upper chip 2 and a lower chip 1 stacked, and the upper chip 2 and the lower chip 1 are bonded. As shown in fig. 2, the top surface of the lower chip 1 is provided with a plurality of first detection grooves 10, and each first detection groove 10 includes a first microfluidic groove 11, a first microfluidic cell 13, a second microfluidic groove 12, and a second microfluidic cell 14, which are sequentially connected. As shown in fig. 3, the bottom surface of the upper chip 2 is provided with a plurality of second detection grooves 20, and each second detection groove 20 includes a third microfluidic groove 21, a third microfluidic groove 23, a fourth microfluidic groove 22, and a fourth microfluidic groove 24, which are sequentially connected. As shown in fig. 4, the first microfluidic cell 11 and the third microfluidic cell 21 are butted to form a first flow channel 4, the first microfluidic cell 13 and the third microfluidic cell 23 are butted to form a reaction chamber 6, the second microfluidic cell 12 and the fourth microfluidic cell 22 are butted to form a second flow channel 5, and the second microfluidic cell 14 and the fourth microfluidic cell 24 are butted to form a waste liquid chamber 7. A plurality of liquid injection holes 3 and a plurality of air holes 8 are formed in the upper chip 2, the liquid injection holes 3 are in one-to-one correspondence connection with the first micro flow grooves 11, and the air holes 8 are in one-to-one correspondence connection with the fourth micro flow grooves 24. The microfluidic chip is divided into an upper chip 2 and a lower chip 1, and the upper chip 2 and the lower chip 1 are combined into a complete microfluidic chip through a conventional bonding process. The surfaces of the upper chip 2 and the lower chip 1 are cleaned and activated during bonding, the surfaces are directly bonded under a certain condition, the upper chip 2 and the lower chip 1 are bonded into a whole through Van der Waals force, molecular force and even atomic force, the use of a sealing element is reduced, the operation convenience is improved, the internal flow channel and cavity characteristics are respectively arranged on the upper part and the lower part, and a complete and closed flow channel and cavity are formed after combination, so that the condition that the environment is polluted by the virus diffused into the air is avoided. The liquid injection hole 3 is connected with the reaction cavity 6 through the first flow passage 4 and is used for liquid feeding operation in the virus detection process. The liquid reacted in the reaction cavity 6 is discharged into the waste liquid cavity 7 through the second flow channel 5, and the air holes 8 are formed in the upper portion of the waste liquid cavity 7, so that the waste liquid can enter the waste liquid cavity 7 conveniently. The micro-fluidic chip can flexibly design the number of internal parallel cavity structures under the allowable chip volume according to the actual requirement, and high flux can be realized.

Further, the first flow passage 4 and the second flow passage 5 are both cylindrical passages. The cross sections of the first micro flow groove 11, the second micro flow groove 12, the third micro flow groove 21 and the fourth micro flow groove 22 are semicircular, the first micro flow groove 11 and the third micro flow groove 21 are butted to form a cylindrical first flow channel 4, and the second micro flow groove 12 and the fourth micro flow groove 22 are butted to form a cylindrical second flow channel 5. The circular flow channel is more beneficial to the flowing of liquid, the residue in the flow channel of the liquid is reduced, and the cylindrical flow channel has no dead angle, so that the cleaning process after the operation is more beneficial.

Further, as shown in fig. 5, the bottoms of the first micro flow groove 11 and the second micro flow groove 12 are at the same level, which facilitates the flow of the liquid; the horizontal height of the bottom of the first microfluidic groove 11 is greater than the horizontal height of the bottom of the first microfluidic cell 13, so that after the sample liquid and the reaction reagent are filled, the sample liquid and the reaction reagent can completely flow into the first microfluidic cell 13 under the action of gravity. The horizontal height of the bottom of the first micro-flow cell 13 is greater than the horizontal height of the bottom of the second micro-flow cell 14, and under the condition that the widths of the first micro-flow cell 13 and the second micro-flow cell 14 are the same, the smaller the horizontal height of the bottom of the first micro-flow cell is, the larger the volume of the micro-flow cell is, and the larger the volume of the second micro-flow cell 14 is, the greater the volume of the first micro-flow cell 13 is, so that the second micro-flow cell 14 can completely contain substances generated by reaction in the first micro-flow cell 13. The bottom of the third microflow groove 21, the bottom of the fourth microflow groove 22, the bottom of the third microflow pool 23 and the bottom of the fourth microflow pool 24 are all at the same horizontal height, so that the sample liquid and the reaction reagent can smoothly flow in each microflow groove.

Next, referring to fig. 1, the liquid injection hole 3 is a tapered hole or a threaded hole, and the diameter of the tapered hole gradually decreases from the upper surface to the lower surface of the upper chip. When the liquid injection hole 3 is a threaded hole, the liquid injection hole 3 is used for connecting a threaded connector and is used for feeding liquid by external power (an injection pump or an air pump and the like); when annotating liquid hole 3 for the gradual change hole, the gradual change hole is hopper-shaped, accuracy when having improved sample liquid and reaction reagent filling, through adopting liquid-transfering gun or syringe to annotating the dropwise add liquid in the liquid hole, the micro-fluidic chip passes through the siphon effect feed liquor to adapt to different use scenes.

Finally, as shown in fig. 2 and 3, the plurality of first detection grooves 10 are distributed at intervals, and the plurality of second detection grooves 20 are distributed at intervals, so that the first detection grooves 10 and the second detection grooves 20 can be accurately butted. The runners formed after butt joint are mutually independent, so that the cross influence is avoided, and the detection precision and efficiency are effectively improved.

The utility model provides a micro-fluidic chip is used for the detection of virus, can also be used to other detection operations. When the detection starts, a detection person injects sample liquid to be detected and a reaction reagent into the first flow channel 4 from the liquid injection hole 3 of the chip through the injection pump, the sample liquid and the reaction reagent enter the reaction cavity 6 along the first flow channel 4 for reaction, waste liquid after the reaction is finished enters the waste liquid pool along the second flow channel 5, and the waste liquid collected by the waste liquid pool is subjected to centralized treatment. In the whole process, the sample liquid, the detection reagent and the waste liquid are all sealed and retained in the chip, so that the pollution of biological waste is greatly reduced. Through a plurality of mutually independent runners in the micro-fluidic chip, the simultaneous implementation of flows such as liquid inlet, reaction, detection of multichannel sample can be realized, and mutually independent can not cross influence moreover, has improved detection efficiency and detection precision effectively.

In the description of the present invention, it is to be understood that the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium; either as communication within the two elements or as an interactive relationship of the two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless otherwise expressly stated or limited, a first feature may be "on" or "under" a second feature, and the first and second features may be in direct contact, or the first and second features may be in indirect contact via an intermediate. Also, a first feature "on," "above," and "over" a second feature may be directly or obliquely above the second feature, or simply mean that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the second feature, or may simply mean that the first feature is at a lower level than the second feature.

In the description herein, the description of the terms "one embodiment," "some embodiments," "an embodiment," "an example," "a specific example" or "some examples" or the like, means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

While embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that modifications, alterations, substitutions and variations may be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims

1. The microfluidic chip for virus detection is characterized by comprising an upper chip and a lower chip which are stacked, wherein the upper chip and the lower chip are connected in a bonding manner;

2. The microfluidic chip for virus detection according to claim 1, wherein the first flow channel and the second flow channel are both cylindrical channels.

3. The microfluidic chip for virus detection according to claim 1, wherein the groove bottoms of the first and second microfluidic grooves are at the same level.

4. The microfluidic chip for virus detection according to claim 1, wherein the level of the bottom of the first microfluidic channel is greater than the level of the bottom of the first microfluidic channel;

5. The microfluidic chip for virus detection according to claim 1, wherein the bottom of the third microfluidic channel, the bottom of the fourth microfluidic channel, the bottom of the third microfluidic cell, and the bottom of the fourth microfluidic cell are all at the same level.

6. The microfluidic chip for virus detection according to claim 1, wherein the liquid injection hole is a tapered hole or a threaded hole, and the diameter of the tapered hole gradually decreases from the upper surface to the lower surface of the upper chip.

7. The microfluidic chip for virus detection according to any one of claims 1 to 6, wherein a plurality of the first detection wells are spaced apart and a plurality of the second detection wells are spaced apart.