CN212787180U

CN212787180U - Gradient cooling device is stored to immunocyte

Info

Publication number: CN212787180U
Application number: CN202020506146.4U
Authority: CN
Inventors: 王阳; 康爽明
Original assignee: Jiahe Hongsheng Shenzhen Health Industry Group Co ltd
Current assignee: Jiahe Hongsheng Shenzhen Health Industry Group Co ltd
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2021-03-26
Anticipated expiration: 2030-04-09

Abstract

The utility model discloses an immunocyte stores gradient cooling device, including the box, the inside of box transversely is equipped with a plurality of baffles, one side lateral wall of box is equipped with the coolant entry, the opposite side lateral wall of box is equipped with the coolant export, the inside of box is equipped with a plurality of infrared radiation thermometer, rotate between the inside upper and lower lateral wall of box and be connected with the threaded rod, two upper chambers lean on the diffusion of lower part cavity air conditioning to reach the effect of refrigeration, and the temperature is the gradient decline from top to bottom, set up infrared radiation thermometer in each cavity, relevant personnel can be clear convenient learn whether the temperature in the corresponding cavity meets the demands, make things convenient for relevant personnel to use the device; the two embodiments are two different implementation forms of test-tube rack, all can realize laying the effect to the good centre gripping of test tube, can choose for use by relevant personnel in a flexible way, and the device is whole, simple structure, and reliable operation, convenient to use and popularization.

Description

Gradient cooling device is stored to immunocyte

Technical Field

The utility model relates to a technical field is stored to the cell, and specific field is an immune cell stores gradient cooling device.

Background

Cell cryopreservation is one of the main methods for cell preservation, and the technology can make cells temporarily depart from growth state and preserve their cell characteristics, so that the cells can be recovered for experiment when necessary, besides, some cells can be purchased, hosted, exchanged and transported by using cell cryopreservation mode, the standard freezing speed is-1 to-2 ℃/min, and can be accelerated when the temperature is lower than-25 ℃, and can be directly thrown into liquid nitrogen (-196 ℃) after-80 ℃.

Patent No. CN201721618935.1 discloses a technical scheme of gradient cooling system is stored to immunocyte, and the coolant temperature in first cooling chamber, second cooling chamber, the third cooling intracavity descends in proper order, through this kind of gradient formula temperature variation, and the transport of cooperation pipe support can be effectual accomplishes the gradient cooling and preserve, and this technical scheme exists following weak point: when the test tubes storing the cells are cooled in each cooling cavity, whether the actual temperature of the test tubes meets the requirement or not cannot be known, so that the timing for lifting the control tube rack cannot be well realized; in addition, each cooling cavity is filled with cooling medium, so that the consumption of the cooling medium is high, and unnecessary waste exists. In this regard, a novel gradient cooling device for storing immune cells is proposed to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an immune cell stores gradient cooling device to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: an immune cell storage gradient cooling device comprises a box body, wherein a plurality of partition plates are transversely arranged in the box body, a cooling medium inlet is formed in the side wall of one side of the box body, a cooling medium outlet is formed in the side wall of the other side of the box body, a plurality of infrared thermometers are arranged in the box body, a threaded rod is rotatably connected between the upper side wall and the lower side wall in the box body, one end of the threaded rod penetrates through the box body and is positioned outside the box body, a mounting frame is arranged at the position, corresponding to the threaded rod, of the outer side wall of the box body, a motor is arranged on the inner side wall of the mounting frame, the output end of the motor is connected with the threaded rod, a test tube rack is in threaded connection with the outer part of the threaded rod, a travel rod is connected to the test tube rack, the inside connection of quad slit has glass sleeve pipe, the test-tube rack is located glass sleeve pipe's inside.

Preferably, the lower surface of box is equipped with the support frame, the bottom four corners of support frame is equipped with the universal wheel.

Preferably, the test-tube rack includes first flat board, a plurality of through-holes have been seted up on the first flat board, the lower surface of first flat board is equipped with the one end of connecting rod, the other end of connecting rod is connected with the second flat board, the arc wall has been seted up on the second flat board.

Preferably, the test-tube rack includes that the third is dull and stereotyped, transversely run through the slip on the third flat board and be equipped with the pull rod, the one end of pull rod is connected with the backup pad, correspond on the pull rod the backup pad with position cover between the third flat board lateral wall is equipped with compression spring, the other end of pull rod is equipped with the pull ring, run through on the third flat board and be equipped with a plurality of through-holes, the pull rod corresponds the position of through-hole is connected with the briquetting, the through-hole orientation the arc wall has been seted up on the surface of briquetting.

Preferably, an observation window is arranged on the side wall of one side of the box body.

Preferably, the contact part of the glass sleeve and the partition plate is provided with a sealing gasket.

Preferably, the number of the partition plates is 2.

Compared with the prior art, the beneficial effects of the utility model are that: a kind of immune cell stores the gradient cooling device, the baffle divides the container body into three chambers, only wash into the cooling medium in the lower chamber, two upper chambers lean on the diffusion of the cold air of the lower chamber to achieve the refrigerated effect, and the temperature is in the form of gradient to decline from top to bottom, have solved the problem that the cooling medium is wasted existing in the prior art; an infrared thermometer is arranged in each cavity, so that related personnel can clearly and conveniently know whether the temperature in the corresponding cavity meets the requirements or not, and the related personnel can conveniently use the device; the two embodiments are two different implementation forms of test-tube rack, all can realize laying the effect to the good centre gripping of test tube, can choose for use by relevant personnel in a flexible way, and the device is whole, simple structure, and reliable operation, convenient to use and popularization.

Drawings

Fig. 1 is a schematic front view of the present invention;

fig. 2 is a schematic front sectional view of embodiment 1 of the present invention;

fig. 3 is a schematic view of a partial enlarged structure at a in fig. 2 according to the present invention;

fig. 4 is a schematic top sectional view of embodiment 1 of the present invention;

fig. 5 is a schematic front sectional view of embodiment 2 of the present invention;

fig. 6 is a schematic view of a partial enlarged structure at B in fig. 5 according to the present invention;

fig. 7 is a schematic top sectional view of embodiment 2 of the present invention;

fig. 8 is a schematic diagram of a partial enlarged structure at C in fig. 7 according to the present invention.

In the figure: 1-box body, 2-partition board, 3-cooling medium inlet, 4-cooling medium outlet, 5-infrared thermometer, 6-threaded rod, 7-mounting rack, 8-motor, 9-test tube rack, 901-first flat plate, 902-connecting rod, 903-second flat plate, 904-third flat plate, 905-pull rod, 906-supporting plate, 907-compression spring, 908-pull ring, 909-press block, 10-stroke rod, 11-glass sleeve, 12-supporting frame, 13-universal wheel and 14-observation window.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Embodiment 1 please refer to fig. 1-4, the present invention provides a technical solution: an immunocyte stores the gradient cooling device, including the container body 1, one side wall of the container body 1 is the opening, the opening department sets up the chamber door, and the container body 1 and material of the chamber door should meet the requirement that the coefficient of heat conductivity is lower, the inside of the container body 1 of horizontally there are several baffles 2, the baffle 2 divides the container body 1 into several chambers, one side wall of the container body 1 has coolant entrance 3, another side wall of the container body 1 has coolant exit 4, coolant entrance 3 and coolant exit 4 correspond to the lowest chamber of the container body 1, the coolant can be liquid nitrogen, the temperature of several upper chambers is reduced sequentially because of the physical characteristic from top to bottom, the temperature of each chamber has certain difference because of the difference of the size of the container body 1 and coefficient of heat conductivity of the internal baffle 2 of the container body 1, the existence of this difference can meet the requirement of gradient cooling of the cell exactly, the box body 1 is internally provided with a plurality of infrared thermometers 5, the infrared thermometers 5 are distributed in each cavity and used for measuring the temperature when corresponding to the cavity, the outer side wall of the box body 1 is provided with a liquid crystal display screen which is used for displaying the temperature measured by the infrared thermometers 5, a threaded rod 6 is rotatably connected between the upper side wall and the lower side wall in the box body 1, a bearing can be arranged at the rotating connection part, a flange is arranged outside the bearing and used for positioning the bearing, one end of the threaded rod 6 penetrates through the box body 1 and is positioned outside the box body 1, the outer side wall of the box body 1 is provided with a mounting frame 7 corresponding to the position of the threaded rod 6 and can be fixedly connected through a bolt, the inner side wall of the mounting frame 7 is provided with a motor 8, the motor 8 needs to be a servo motor so as to drive the threaded rod 6 to rotate forwards, the form that sets up of test-tube rack 9 has detailed below, runs through sliding connection on the test-tube rack 9 and has stroke rod 10, and the two is clearance fit between the two, and the both ends of stroke rod 10 link to each other with the upper and lower inside wall of box 1 respectively, and the relation of connection is fixed connection, has seted up the quad slit on the baffle 2, and the internal connection of quad slit has glass sleeve pipe 11, and test-tube rack 9 is located glass sleeve pipe 11's inside.

Particularly, the lower surface of box 1 is equipped with support frame 12, and the bottom four corners of support frame 12 is equipped with universal wheel 13, adds and establishes universal wheel 13 and can improve the holistic mobility of the device, makes things convenient for its removal.

Specifically, the test-tube rack 9 includes first flat board 901, has seted up a plurality of through-holes on the first flat board 901, changes the shape of through-hole and the cross sectional shape looks adaptation of test tube, and its radius rate is greater than the cross-sectional radius of test tube, and the lower surface of first flat board 901 is equipped with the one end of connecting rod 902, and the other end of connecting rod 902 is connected with second flat board 903, has seted up the arc wall on the second flat board 903, the shape looks adaptation of this arc wall and test tube bottom, and this arc wall will possess certain degree of depth.

Particularly, one side wall of the box body 1 is provided with an observation window 14, which is convenient for relevant personnel to observe the condition in the box body 1 in real time.

Specifically, the contact portions of the glass sleeve 11 and the partition plate 2 are provided with gaskets, so that the sealing performance between the glass sleeve 11 and the partition plate 2 is improved.

Specifically, the number of the partition boards 2 is 2, and correspondingly, the number of the corresponding chambers is three, and the number of the infrared thermometers 5 is also 3.

Embodiment 2 referring to fig. 5 to 8, the difference between this embodiment and embodiment 1 is: the test tube rack 9 comprises a third flat plate 904, a pull rod 905 transversely penetrates through the third flat plate 904 in a sliding mode, the cross section of the pull rod 905 is polygonal, and the shape of a stroke hole formed in the third flat plate 904 is the same as that of the pull rod 905. As shown in fig. 6 and 8, the third plate 904 is divided into two plates, which are connected with each other by bolts, one end of a pull rod 905 is connected with a support plate 906, a compression spring 907 is sleeved on the pull rod 905 at a position corresponding to a position between the side walls of the support plate 906 and the third plate 904, the other end of the pull rod 905 is provided with a pull ring 908, the third plate 904 is provided with a plurality of through holes in a penetrating manner, the pull rod 905 is connected with a press block 909 at a position corresponding to the through holes, and the through holes are provided with arc-shaped.

The working principle is as follows: when the utility model is used, firstly, the test tube with the cell mixed liquid is placed on the test tube rack 9, after the test tube is placed in the through hole in the embodiment 1, the bottom of the test tube is placed in the arc-shaped groove on the first plate, the pull ring 908 is pulled in the embodiment 2, the pull ring 908 drives the pull rod 905 to move, the press block 909 moves along with the pull rod 905, the compression spring 906 is compressed by the support plate 907 and stores a certain elastic potential energy, at the moment, the test tube with the cell mixed liquid is placed between the press block 909 and the arc-shaped groove on the third plate 904, then the pull ring 908 is loosened, the elastic potential energy stored by the compression spring 906 is released, and the pull rod 905 is driven to move leftwards, the press block 909 can reliably clamp the test tube, compared with the embodiment 1, the clamping effect of the test tube rack 9 disclosed in the embodiment 2 is better, the occupied volume is smaller, but the manufacturing cost and the process cost are relatively, can be flexibly selected by related personnel. After the test tube is completely held, liquid nitrogen is injected from the cooling medium inlet 3, after the temperature in each chamber displayed by the liquid crystal screen is proper, the motor 8 is started, and the test tube rack 9 slowly moves downwards along the stroke rod 10 under the action of the threaded rod 6, so that the slow reduction of the temperature of cells in the test tube can be realized.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The utility model discloses the standard part that uses all can purchase from the market, and dysmorphism piece all can be customized according to the record of description and attached drawing, and the concrete connection mode of each part all adopts conventional means such as ripe bolt, rivet, welding among the prior art, and machinery, part and equipment all adopt prior art, and conventional model is including the conventional connected mode of circuit connection adoption conventional among the prior art, and here detailed description is not again.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an immune cell stores gradient cooling device, includes box (1), its characterized in that: the box body (1) is internally and transversely provided with a plurality of partition plates (2), one side wall of the box body (1) is provided with a cooling medium inlet (3), the other side wall of the box body (1) is provided with a cooling medium outlet (4), the box body (1) is internally provided with a plurality of infrared thermometers (5), the upper side wall and the lower side wall of the interior of the box body (1) are rotatably connected with a threaded rod (6), one end of the threaded rod (6) penetrates through the box body (1) and is positioned outside the box body (1), the outer side wall of the box body (1) is provided with a mounting frame (7) corresponding to the threaded rod (6), the inner side wall of the mounting frame (7) is provided with a motor (8), the output end of the motor (8) is connected with the threaded rod (6), and the exterior of the threaded rod (6) is, run through sliding connection on test-tube rack (9) and have stroke pole (10), the both ends of stroke pole (10) respectively with the upper and lower inside wall of box (1) links to each other, the quad slit has been seted up on baffle (2), the internal connection of quad slit has glass sleeve pipe (11), test-tube rack (9) are located the inside of glass sleeve pipe (11).

2. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: the lower surface of box (1) is equipped with support frame (12), the bottom four corners of support frame (12) is equipped with universal wheel (13).

3. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: the test tube rack (9) comprises a first flat plate (901), a plurality of through holes are formed in the first flat plate (901), one end of a connecting rod (902) is arranged on the lower surface of the first flat plate (901), the other end of the connecting rod (902) is connected with a second flat plate (903), and an arc-shaped groove is formed in the second flat plate (903).

4. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: the test-tube rack (9) includes that the third is dull and stereotyped (904), it is equipped with pull rod (905) to transversely run through the slip on the third is dull and stereotyped (904), the one end of pull rod (905) is connected with backup pad (906), correspond on pull rod (905) backup pad (906) with position cover between the dull and stereotyped (904) lateral wall of third is equipped with compression spring (907), the other end of pull rod (905) is equipped with pull ring (908), it is equipped with a plurality of through-holes to run through on the third is dull and stereotyped (904), pull rod (905) correspond the position of through-hole is connected with briquetting (909), the through-hole orientation the arc wall has been seted up on the surface of briquetting (909).

5. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: an observation window (14) is arranged on the side wall of one side of the box body (1).

6. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: and sealing gaskets are arranged at the contact parts of the glass sleeve (11) and the partition plate (2).

7. The gradient cooling device for storing immune cells as claimed in claim 1, wherein: the number of the partition boards (2) is 2.