CN212713526U - Bone-derived alkaline phosphatase quantitative determination reagent strip, kit and detection instrument - Google Patents
Bone-derived alkaline phosphatase quantitative determination reagent strip, kit and detection instrument Download PDFInfo
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- CN212713526U CN212713526U CN202020921297.6U CN202020921297U CN212713526U CN 212713526 U CN212713526 U CN 212713526U CN 202020921297 U CN202020921297 U CN 202020921297U CN 212713526 U CN212713526 U CN 212713526U
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Abstract
The utility model belongs to the technical field of the medical treatment detects technique and specifically relates to a bone source nature alkaline phosphatase quantitative determination reagent strip, this detection reagent strip includes reaction plate, reaction hole, reaction film, transition pad and water absorption pad, and this reaction plate is bone source nature alkaline phosphatase reaction plate, and this reaction film is bone source nature alkaline phosphatase-nitrocellulose reaction film, and the central authorities above the reaction film are equipped with the detection zone, and this detection zone comprises binding protein and mixed nitrocellulose; the reaction plate is provided with reaction holes, the reaction membrane is arranged at the reaction holes below the reaction plate, the water absorption pad is arranged below the reaction membrane, and the transition pad is arranged above the reaction holes; the detection reagent strip can be used for a kit and a detection instrument. The utility model has the advantages of being simple in structure and simple in operation, the sample volume is few, and patient compliance is better, is particularly useful for children, and has improved the sensitivity and the accuracy that detect, has the advantage in the aspects such as detection accuracy, consuming time, security, detection price and operation.
Description
Technical Field
The utility model relates to a medical treatment detects technical field, especially relates to a bone source nature alkaline phosphatase quantitative determination reagent strip, kit and detecting instrument.
Background
The onset process of rickets is a chronic process, the clinical manifestation in the initial stage has no specificity, the treatment is carried out until obvious bone changes appear, the optimal period of the treatment is missed, and the growth and development of children are adversely affected. Therefore, in clinical work, an index is needed to measure the treatment level, so as to prevent under-treatment or over-treatment, misdiagnosis and missed diagnosis are easily caused by simply depending on symptoms and signs, and the conventional blood calcium, phosphorus, whole blood alkaline phosphatase and X-ray detection have low sensitivity and poor specificity, so that the method is not applied to early diagnosis.
The bone-derived alkaline phosphatase (NBAP) is one of phenotype markers of osteoblasts, is secreted from bone, is secreted more when the calcium salt precipitation in bone is insufficient, and is secreted less when the calcium salt in bone is sufficient, so that the activity or the functional condition of osteoblasts can be directly reflected. The method for detecting the catalytic activity of the bone-derived alkaline phosphatase in the blood of the children so as to screen or assist in diagnosing the bone calcification disorder caused by calcium malnutrition or the metabolic bone disease caused by other reasons is a specific reference index mainly used for early diagnosis and subclinical identification of the rickets in the children in recent years and is also an optimal index currently used for evaluating the bone mineralization disorder of human bodies.
In the prior art, the main detection methods of the content of the bone alkaline phosphatase in the serum comprise an electrophoresis method, a lectin agglutination method, an enzyme-linked immunosorbent assay and the like. However, these detection methods either require complicated instruments or serum as a sample, and therefore have the disadvantages of large blood consumption, complicated detection procedures, slow detection speed, etc., and are not suitable for the bone alkaline phosphatase-abnormally high-incidence population of infants.
In addition, the current technology has no reagent for detecting the content of the ferritin by sampling once (taking peripheral blood), and cannot accurately give the content of the ferritin. The method has the advantages of large sampling amount, inconvenient operation of doctors and patients, complex biochemical detection, long consumed time, low accuracy and sensitivity, incapability of judging the severity of anemia, and frequent diagnosis mainly by visual observation in the market, which causes errors, influences judgment of doctors and cannot provide powerful reference.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned problem that exists, the utility model aims at providing a simple structure, simple operation, the sample volume is few, and patient compliance is better, is particularly useful for children, and once sample can detect out bone source nature alkaline phosphatase quantitative determination reagent strip of protein content, the utility model provides a bone source nature alkaline phosphatase quantitative determination reagent strip, including reaction plate, reaction hole, reaction membrane, transition pad and absorbent pad, the reaction plate is bone source nature alkaline phosphatase reaction plate, the central authorities above the reaction membrane are equipped with the detection zone; the reaction plate is provided with reaction holes, the reaction membrane is arranged at the reaction hole below the reaction plate, the water absorption pad is arranged below the reaction membrane, and the transition pad is arranged above the reaction holes.
Preferably, the reaction hole is one of a circle or a square.
Preferably, the detection area coincides with the reaction hole, so that the detection is convenient.
Preferably, a protective paper is adhered to one surface of the reaction plate, the reaction membrane is adhered to the side of the adhesive surface of the reaction plate from which the protective paper is removed, the reaction membrane can cover the reaction holes, and an adhesive part is arranged outside the detection area of the reaction membrane.
Preferably, be equipped with the through-hole on the transition pad, just the through-hole coincides with the reaction hole, is convenient for advance a kind and observe.
Preferably, the absorbent pad has 3 layers, so that the absorption effect can be improved.
In order to achieve the above object, the present invention further provides a kit, which comprises the reagent strip for quantitatively detecting bone-derived alkaline phosphatase as described above. Preferably, the kit comprises an upper clamping shell and a lower clamping shell, the upper clamping shell is provided with a sample adding hole and an observation port, the sample adding hole is arranged at the corresponding position of the reaction hole, the observation port and the sample adding hole are arranged in parallel in the front and at the back, and the size of the sample adding hole and the size of the observation port are matched with that of the reaction hole; the lower clamping shell is provided with a clamping groove for placing a water absorption pad on the detection reagent strip and fixing the detection reagent strip, and the clamping groove is fixed in the sliding rails on the front side and the rear side inside the lower clamping shell, so that the clamping groove can move in the sliding rails.
In order to achieve the above object, the utility model provides a detecting instrument, detecting instrument and as above the bone source nature alkaline phosphatase quantitative determination reagent strip on detection zone electric connection, just be provided with data storage device in the detecting instrument, the data storage device in-storage has the calibration curve.
The utility model has the advantages that:
this scheme simple structure, simple operation, the sample volume, few patient compliance is better, is particularly useful for children to once take a sample, just can detect out what protein content is what, this testing reagent strip has improved its sensitivity and accuracy, has the advantage in the aspect of detection accuracy, consuming time, security, detection price and operation etc..
Drawings
FIG. 1 is a schematic diagram of the overall structure of the reagent strip, the kit and the detection instrument for quantitative detection of bone-derived alkaline phosphatase.
Fig. 2 is a schematic view of a front view structure of a detection reagent strip in embodiment 1 of the present invention.
Fig. 3 is a schematic structural diagram of the detection kit according to embodiment 2 of the present invention.
FIG. 4 is a schematic diagram showing the structure of the bone-derived alkaline phosphatase-nitrocellulose reaction membrane in a plan view.
FIG. 5 is a schematic top view of the bone-derived alkaline phosphatase reaction plate.
In the figure: 1-detection reagent strip, 2-kit, 3-detection instrument, 4-reaction plate, 5-reaction hole, 6-reaction membrane, 7-detection zone, 8-transition pad, 9-water absorption pad, 10-upper card shell, 11-lower card shell, 12-sample hole, 13-observation port, 14-card slot, 15-adhesive part and 16-slide rail.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Detailed description of the preferred embodiment 1
Fig. 1 and fig. 2 are schematic structural diagrams of the bone-derived alkaline phosphatase quantitative determination reagent strip of the present invention. As shown in fig. 2, a schematic structural diagram of a bone-derived alkaline phosphatase quantitative determination reagent strip includes a detection reagent strip 1, which includes a reaction plate 4, reaction holes 5, a reaction membrane 6, a transition pad 8 and a water absorption pad 9; the reaction plate is an osteogenic alkaline phosphatase reaction plate, and the reaction membrane is an osteogenic alkaline phosphatase-nitrocellulose reaction membrane. Be equipped with the circular shape reaction hole on the reaction plate, the one side glue of reaction plate has the protection paper, tears the protection paper, can paste the reaction membrane in reaction hole department, and this reaction membrane can cover the reaction hole, is divided into detection zone 7 and sticky part 15 on the reaction membrane, and the detection zone sets up the central authorities in the reaction membrane top, and this detection zone comprises binding protein and mixed nitrocellulose, and this detection zone and reaction hole coincidence. The water absorption pad is provided with 3 layers and is placed below the reaction membrane; the transition pad is arranged above the reaction hole, and a through hole is arranged on the transition pad and coincides with the reaction hole.
Specific example 2
Fig. 3 is a schematic structural diagram of the detection kit according to embodiment 2 of the present invention. The embodiment provides a kit 2, which comprises the above-mentioned reagent strip for quantitatively detecting bone-derived alkaline phosphatase, and specifically comprises an upper card shell 10 and a lower card shell 11, wherein the upper card shell is provided with a sample adding hole 12 and an observation port 13, the sample adding hole is arranged at a corresponding position of a reaction hole, the observation port and the sample adding hole are arranged in parallel in the front and at the back, and the sizes of the sample adding hole and the observation port are matched with the reaction hole; the lower card shell is provided with a clamping groove 14 for placing a water absorption pad on the detection reagent strip to fix the detection reagent strip, and the clamping groove is fixed in slide rails 16 at the front side and the rear side inside the lower card shell, so that the clamping groove can move in the slide rails.
Specific example 3
The embodiment provides a detecting instrument 3, which is electrically connected to the detection area on the reagent strip for quantitatively detecting bone-derived alkaline phosphatase, and a data storage device is arranged in the detecting instrument, and a calibration curve is stored in the data storage device.
And (3) checking the principle: the detection area consists of binding protein on an osteogenic alkaline phosphatase-nitrocellulose reaction membrane (NBAP-NC reaction membrane) and a mixed nitrocellulose membrane, and the osteogenic alkaline phosphatase is specifically detected by applying an immunochromatography principle. When the bone-derived alkaline phosphatase is added into the sample adding hole, the bone-derived alkaline phosphatase in the sample reacts with the binding substance in the test strip on the NBAP-NC reaction membrane, and a colored substance is formed in the reaction area after the color developing solution is added. And (3) measuring the color development intensity of the detection area by a matched analyzer, detecting the signal intensity of the NBAP-NC reaction membrane test area in the reaction hole, and calculating to obtain an alkaline phosphatase (bone origin) detection result by a calibration curve stored on a bar code or an SD card.
And (3) a checking process: before use, the NBAP-NC reaction membrane was first pretreated: washing the NBAP-NC reaction membrane by using a NBAP-NC equilibrium solution (ethanesulfonic acid buffer solution) until the washing equilibrium is reached, and then naturally drying; coating with buffer solution containing lectin, spotting the coating solution on NBAP-NC reaction membrane, and naturally drying; and sealing the NBAP-NC reaction membrane, treating the NBAP-NC reaction membrane with sealing liquid, placing the NBAP-NC reaction membrane on clean filter paper, storing the NBAP-NC reaction membrane at 2-8 ℃ overnight, and taking out the NBAP-NC reaction membrane the next day to obtain the well-treated NBAP-NC reaction membrane.
Then, the detection area is pretreated: taking an osteogenic alkaline phosphatase reaction plate (NBAP reaction plate), uncovering the glue surface and the protective paper, pasting the prepared NBAP-NC reaction membrane on the glue surface with the detection surface upward to obtain the prepared NBAP reaction plate, pasting the NBAP-NC reaction membrane on the NBAP reaction plate at the position of a reaction hole, covering the reaction hole, centering the NBAP-NC reaction membrane, and enabling the detection region on the NBAP-NC reaction membrane to coincide with the circular reaction hole, thereby being beneficial to observation and test after liquid (whole blood or serum) chromatography.
Next, taking the detection card shell, putting the (NBAP) water absorption pad (3 layers) into the clamping groove, putting the NBAP reaction plate at the position of the clamping groove of the push-pull plate, with the front surface upward, and putting the NBAP reaction plate above the water absorption pad in the detection card shell; the transition pad is placed above the reaction hole, and the upper shell and the lower shell are tightly pressed by a shell pressing machine after the upper cover (the upper clamping shell) is buckled.
During detection, the NBAP reaction membrane is washed, then a whole blood or serum sample is added to contact with the NBAP-NC reaction membrane and react with substances on the NBAP-NC reaction membrane, then the NBAP reaction membrane is washed, after no obvious liquid exists on the NBAP-NC reaction membrane, the NBAP reaction plate is pushed, the clamping groove moves in the sliding rail, the NBAP-NC reaction membrane moves to the middle position of the observation port, a matched analyzer is used for measuring the color development intensity of the reaction area, and the concentration of the substances to be detected is obtained quantitatively.
To sum up, the utility model discloses an osteogenic alkaline phosphatase quantitative determination reagent strip, detection box and detecting instrument that above-mentioned embodiment discloses, simple structure, simple operation, the sample volume is few, and patient compliance is better, is particularly useful for children to once take a sample, just can detect out protein content what, this detection reagent strip has improved its sensitivity and accuracy, has the advantage in the aspect of detection accuracy, consuming time, security, detection price and operation etc..
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can understand the changes or substitutions within the technical scope of the present invention, and the protection scope of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. The quantitative detection reagent strip for bone-derived alkaline phosphatase is characterized in that: detect reagent strip (1) including reaction plate (4), reaction hole (5), reaction membrane (6), transition pad (8) and absorb water pad (9), the reaction plate is bone source nature alkaline phosphatase reaction plate, the central authorities of reaction membrane top are equipped with detection zone (7), be equipped with the reaction hole on the reaction plate, the reaction membrane sets up reaction hole department in the reaction plate below, the pad setting that absorbs water is in the below of reaction membrane, the transition pad sets up the top in the reaction hole.
2. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 1, wherein: the reaction hole is one of a circle or a square.
3. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 1 or 2, wherein: the detection zone coincides with the reaction well.
4. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 1, wherein: and protective paper is glued on one surface of the reaction plate, the reaction film is stuck on one side of the glue surface of the reaction plate after the protective paper is removed, and the reaction film can cover the reaction holes.
5. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 4, wherein: outside the detection zone of the reaction membrane is a glued section (15).
6. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 1, wherein: the transition pad is provided with a through hole, and the through hole is superposed with the reaction hole.
7. The reagent strip for quantitatively detecting bone-derived alkaline phosphatase according to claim 1, wherein: the absorbent pad is 3 layers.
8. A kit, characterized in that: the kit (2) comprises the bone-derived alkaline phosphatase quantitative determination reagent strip according to any one of claims 1 to 7.
9. The kit of claim 8, wherein: the kit comprises an upper clamping shell (10) and a lower clamping shell (11), wherein the upper clamping shell is provided with a sample adding hole (12) and an observation port (13), the sample adding hole is arranged at the corresponding position of a reaction hole, the observation port and the sample adding hole are arranged in parallel in the front and at the back, and the sizes of the sample adding hole and the observation port are matched with the reaction hole; the lower clamping shell is provided with a clamping groove (14) for placing a water absorption pad on the detection reagent strip and fixing the detection reagent strip, and the clamping groove is fixed in sliding rails (16) on the front side and the rear side inside the lower clamping shell.
10. A detection instrument, characterized by: the detecting instrument (3) is electrically connected with a detecting area on the reagent strip for quantitatively detecting the bone-derived alkaline phosphatase as claimed in any one of claims 1 to 7, and a data storage device is arranged in the detecting instrument, and a calibration curve is stored in the data storage device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020921297.6U CN212713526U (en) | 2020-05-27 | 2020-05-27 | Bone-derived alkaline phosphatase quantitative determination reagent strip, kit and detection instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020921297.6U CN212713526U (en) | 2020-05-27 | 2020-05-27 | Bone-derived alkaline phosphatase quantitative determination reagent strip, kit and detection instrument |
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| Publication Number | Publication Date |
|---|---|
| CN212713526U true CN212713526U (en) | 2021-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020921297.6U Active CN212713526U (en) | 2020-05-27 | 2020-05-27 | Bone-derived alkaline phosphatase quantitative determination reagent strip, kit and detection instrument |
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| CN (1) | CN212713526U (en) |
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- 2020-05-27 CN CN202020921297.6U patent/CN212713526U/en active Active
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