CN212592221U - Body fluid collecting, preprocessing and storing device - Google Patents

Abstract

The utility model discloses a body fluid is gathered, is preliminary treatment and save set, include: the biological sample protective agent comprises a sampling tube, a push cylinder sliding in the sampling tube, a filter assembly arranged at the bottom of the sampling tube, a collecting tube connected with the filter assembly and a biological sample protective agent; the filter assembly includes: the porous high polymer filter plate is arranged in the sampling pipe and is connected with the filter head at the lower end of the sampling pipe; use the utility model discloses a device can once only eliminate the interference of mucin, bacterium, cell debris, oral cavity insoluble substance impurity under the low resistance condition, accomplishes preliminary purification process, improves the stability of the biomolecule in the body fluid sample, especially metabolism class molecule under non-low temperature state.

Description

Body fluid collecting, preprocessing and storing device

Technical Field

The utility model relates to a detect and inspection apparatus field, especially a body fluid is gathered, is preliminary treatment and save set.

Background

Saliva is a viscous liquid secreted by a plurality of salivary glands in the oral cavity, and the main components of the saliva include a large amount of proteins, polypeptides, endogenous metabolites and inorganic ions in addition to water, and human DNA is also contained in exfoliated cells in the oral cavity. Changes in the saliva composition have been shown by several studies to be closely related to chronic diseases and cancer. The amino acids, fatty acids, urea, glucose, choline and derivatives thereof in saliva can intuitively reflect the activity levels of amino acid metabolism, lipid metabolism, urinary circulation, glycolysis, choline metabolism and the like of a human body from the metabolic level, so that whether the human body suffers from metabolic diseases such as cancer, diabetes, chronic nephropathy, Alzheimer's disease and the like is further reflected, and the saliva can be used as a sensitive biomarker. The detection of the saliva is beneficial to early screening, early diagnosis and early intervention of diseases. In a plurality of human body fluid types, saliva is a biological sample which is most easily collected and has the highest comfort level, is a completely noninvasive sample, has low requirement on the specialty degree, and is beneficial to improving the compliance of patients and reducing the diagnosis and treatment pressure, so that the saliva is more and more paid attention to clinically.

The reliability of the biological information reflected by the saliva sample depends to a large extent on a reasonably specified sampling process and a pre-processing, storage process. Due to the complex composition of saliva samples, including not only the above molecules, but also over 1000 kinds of bacteria, the metabolic processes of which can seriously affect the composition of endogenous metabolic molecules and polypeptides in saliva. Cell debris, insoluble substances and impurities in the oral cavity are more likely to pollute saliva samples, and a large amount of mucin in the saliva is difficult to remove through operations such as centrifugation, so that the subsequent extraction and enrichment of other biomolecules are seriously influenced. Particularly, when the subsequent detection object is a metabolic molecule, the interference of various substances such as bacteria, cell debris, mucin, oral insoluble substances and the like needs to be eliminated simultaneously, and then a clear and clean effective saliva sample with good stability can be obtained. The market urgently needs a saliva collection device which can be applied to the conventional detection samples of home collection, non-low-temperature storage and transportation, is easy to operate, high in efficiency and good in stability, and can integrate collection, pretreatment and storage.

The most common saliva collecting devices on the market today are collecting tubes with funnel fittings, which generally improve the stability of saliva samples by crushing the DNA protecting agent inside the collecting tube to mix it with the sample or by adding a DNA protecting agent, such as the ones mentioned in patents CN210347197U, CN 209032444U. The device collects non-irritant saliva passively generated by a human body, but saliva samples collected by the device are high in viscosity, cannot exclude interference of bacteria, cell debris and mucin, contain more oral impurities, cannot guarantee a proper proportion when the collected saliva is directly added into DNA preservation solution, and cannot be suitable for stability control of small molecular substances such as metabolism and polypeptide. Patent CN206523325U discloses a filter membrane-containing saliva collection and storage device, wherein a filter membrane with a pore size of 200nm is attached to the bottom of the device, and a saliva sample in the storage device is collected and passed through the filter membrane by squeezing. Although the method can remove bacteria and material residues, the method is not practical because the viscous protein in saliva has high viscosity and is easy to block a filter membrane.

Another large class of saliva collecting devices in the market need to be matched with sampling adsorption cotton or an adsorbent for saliva collection, developed by SARSTEDT company

The saliva collecting device is characterized in that the absorbent cotton is plugged into the oral cavity to adsorb a sufficient amount of saliva samples, and then the absorbent cotton is placed in a special centrifuge tube to be subjected to high-speed centrifugation, so that clear liquid samples can be obtained. The mode can be operated only by matching with a centrifugal machine, is only suitable for unified treatment in a laboratory, and cannot be applied to scenes without professional equipment. US7618591B2, US7850922B2, US8641642B2 and other patents all disclose another collection device capable of obtaining clarified saliva without centrifugation, i.e. glass fiber material or other adsorbent with strong adsorption force is adoptedThe inlet chamber absorbs saliva and the absorbed liquid is squeezed into the collecting tube by means of pressure. This type of sampling device can adsorb mucin by means of an adsorbent, but also cannot eliminate interference from bacteria. The collection devices disclosed in patents US10023903B2, CN206486520U, CN209734017 add a filter tube or a filter membrane on the basis of the above, so as to simultaneously eliminate the interference of mucin, bacteria, impurities, etc., but such devices containing a sampling adsorbent easily cause an irritant reaction because a sampling head needs to be extended into the oral cavity, which can satisfy the collection and detection of DNA, etc., but interfere the components of the molecules such as metabolic molecules, polypeptides, etc. in the oral cavity, causing inconsistency of results, and the adsorbent can dissolve out small molecules under the action of a body fluid sample, thereby affecting the reliability of biological information.

Therefore, how to provide a saliva collecting, preprocessing and storing device, which can ensure that saliva secretion is not interfered by stimulation of external sampling cotton and the like, can integrally eliminate interference of mucin, bacteria, cell debris and oral insoluble substances/impurities with low resistance, completes a primary purification process, so as to improve the stability of metabolic molecule groups in a non-low temperature state, and becomes a problem to be solved by technical personnel in the field; the utility model provides such a problem.

SUMMERY OF THE UTILITY MODEL

For solving prior art's not enough, the utility model aims to provide a body fluid is gathered, preliminary treatment and save set uses the utility model discloses a device can once only eliminate the interference of mucin, bacterium, cell piece, the insoluble substance in oral cavity/impurity under the low resistance condition, accomplishes preliminary purification process, improves the biomolecule in the body fluid sample, especially metabolism class molecule stability under non-low temperature state.

In order to achieve the above object, the utility model adopts the following technical scheme:

a body fluid collection, pretreatment and preservation device comprising: the biological sample protective agent comprises a sampling tube, a push cylinder sliding in the sampling tube, a filter assembly arranged at the bottom of the sampling tube, a collecting tube connected with the filter assembly and a biological sample protective agent; the filter assembly includes: the porous high polymer filter plate is arranged in the sampling pipe and is connected with the filter head at the lower end of the sampling pipe.

In the body fluid collecting, pretreating and storing device, the porous high polymer filter plate is a hydrophobic porous high polymer filter plate, the plane size of the hydrophobic porous high polymer filter plate is the same as the inner diameter of the sampling tube, the thickness is 1-3mm, and the pore diameter is 10-100 mu m.

In an embodiment, the filter head includes: the filter head body is arranged on the low-adsorption microporous filter membrane in the filter head body; the pore diameter of the low-adsorption microporous filter membrane is 0.2-0.5 mu m.

The collecting tube comprises: the collecting pipe body, the sharp bottom that forms in the collecting pipe body bottom, connect the tube cap of collecting pipe body, the scale that forms in the outer wall of collecting pipe body.

In the body fluid collecting, preprocessing and storing device, the collecting pipe body is connected to the sampling pipe through the threads.

In the body fluid collecting, pretreating and storing device, the pipe opening of the collecting pipe body is larger than the diameter of the liquid outlet of the filter head.

The body fluid collecting, preprocessing and storing device further comprises: the sampling funnel is movably connected with the sampling tube.

The aforesaid body fluid is gathered, is pretreated and save set, and the connected mode between sampling pipe and the sampling funnel includes: screw connection and gluing connection.

In the aforementioned device for collecting, pretreating and storing body fluid, the biological sample protective agent comprises: methanol, ethanol, acetonitrile, acetone, and a mixture of methanol and acetonitrile.

In the body fluid collecting, pretreating and storing device, the volume ratio of the biological sample protective agent to the sample is 2:1-5: 1.

The utility model discloses an useful part lies in:

the utility model provides a body fluid is gathered, is handled in advance, save set can gather non-irritability saliva sample under the condition of not introducing sampling cotton, sampling adsorbent, once only eliminates the interference of mucin, bacterium, cell debris, oral cavity insoluble substance/impurity under the low resistance condition, accomplishes preliminary purification process, need not centrifuge equipment and other laboratory professional equipment during;

the utility model discloses a body fluid collecting device which can realize the collection of saliva samples with relatively stable volume and improve the consistency of the collected saliva samples;

the device of the utility model can be fixed in an integrated way, can be disassembled by threaded connection, has small occupied volume, and is easy to place, store and unify subsequent treatment;

the body fluid collecting, preprocessing and storing device provided by the utility model is simple in operation and high in efficiency, is suitable for non-professional people to operate, and has expansibility on sample types and sample detection objects;

the body fluid sample collected by the body fluid collecting device of the utility model does not need to be preserved at low temperature;

the utility model discloses a body fluid collection system possesses the filtering component who has substep filtering action, can once only filter simultaneously and fall adhesive protein, cell piece, impurity, oral cavity insoluble and bacterium, consequently can reduce the storage condition, can keep stability more than a week under non-low temperature storage condition, is fit for non-centralized sampling, preliminary treatment and save.

Drawings

FIG. 1 is a schematic view of a body fluid collection device according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of an embodiment of the sampling tube of the present invention;

FIG. 3 is a metabolic spectrum of saliva sample collected by the body fluid collecting device of the present invention, wherein the abscissa is mass-to-charge ratio (m/z) and has no unit, and the ordinate is peak intensity and has no unit;

fig. 4 is a mass spectrum comparison chart of saliva samples collected by the body fluid collecting device and the conventional collecting device (funnel cooperation collecting pipe), wherein a is a spectrogram of continuous 3 tubes of saliva samples collected by the funnel cooperation collecting pipe, B is a spectrogram of continuous 3 tubes of saliva samples collected by the body fluid collecting device, the abscissa is mass-to-charge ratio (m/z), and the ordinate is peak intensity and no unit;

FIG. 5 is a comparison graph of mass spectra of a subject passing through a body fluid collecting apparatus comprising a hydrophilic porous polymer filter plate and a hydrophobic porous polymer filter plate, wherein A is a hydrophilic sieve plate, B is a hydrophobic sieve plate, the abscissa is mass-to-charge ratio (m/z), and there is no unit, and the ordinate is peak intensity, and there is no unit;

FIG. 6 is a mass spectrum of a saliva sample collected by a subject through a body fluid collection device without a filter plate but with a filter tip comprising a microporous filter membrane, with mass to charge ratio (m/z) on the abscissa, no units, and peak intensity on the ordinate, no units;

FIG. 7 is a mass spectrum variation coefficient of a saliva sample collected by the body fluid collecting device of the present invention under 3 different storage conditions;

fig. 8 is a representative spectrogram and a variation coefficient of saliva samples collected by the body fluid collecting device of the present invention are placed at different times, the abscissa of the spectrogram is mass-to-charge ratio (m/z) and has no unit, and the ordinate is peak intensity and no unit.

The meaning of the reference symbols in the figures:

1 sampling tube, 2 push cylinder, 21 head, 22 tail, 3 filter component, 31 porous high polymer filter plate, 32 filter head, 321 low-adsorptivity microporous filter membrane, 4 collecting tube, 41 tube cover, 5 biological sample protective agent and 6 sampling funnel.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.

The body fluid of the present invention includes: saliva, sweat, urine, sputum, and other body fluid samples.

A body fluid collection, pretreatment and preservation device as shown in fig. 1, comprising: the biological sample protective agent comprises a sampling tube, a push cylinder sliding in the sampling tube, a filter assembly arranged at the bottom of the sampling tube, a collecting tube connected with the filter assembly and a biological sample protective agent; the filter assembly includes: the porous high polymer filter plate is arranged in the sampling pipe and is connected with the filter head at the lower end of the sampling pipe.

The porous polymer filter plate can be a hydrophilic porous polymer filter plate or a hydrophobic porous polymer filter plate; as shown in fig. 2, the porous polymer filter plate is preferably a hydrophobic porous polymer filter plate, and the hydrophobic porous polymer filter plate is one of porous high molecular weight polyethylene and porous high molecular weight polypropylene. The plane size of the hydrophobic porous polymer filter plate is the same as the inner diameter of the sampling tube, the thickness is 1-3mm, and the aperture is 10-100 μm. Can intercept and adsorb mucin, cell debris and insoluble substances in a body fluid sample such as saliva, liquid entering the sampling tube under the action of no pressure cannot automatically pass through the porous polymer filter plate, and the body fluid sample is automatically intercepted above the porous polymer filter plate at the bottom of the sampling tube during sampling.

Compared with a hydrophilic porous polymer filter plate, the hydrophobic porous polymer filter plate can ensure that liquid in the sampling pipe cannot automatically pass through the porous polymer filter plate under the action of no external pressure, and a body fluid sample is automatically intercepted above the porous polymer filter plate at the bottom of the sampling pipe during sampling, so that the volume of the body fluid sample entering the sampling pipe can be observed more visually; meanwhile, the hydrophobic porous polymer filter plate has low adsorptivity for small molecule metabolites in body fluid, and can ensure the consistency of samples passing through the bulk fluid collection device to the maximum extent.

As shown in fig. 2, the filter head includes: the filter head body is arranged on the low-adsorption microporous filter membrane in the filter head body; the pore diameter of the low-adsorption microporous filter membrane is 0.2-0.5 mu m. Can filter bacteria and viscous substances which are not filtered by the porous polymer filter plate.

The collecting pipe includes: the collecting pipe body, the sharp bottom that forms in the collecting pipe body bottom, connect the tube cap of collecting pipe body, the scale that forms in the outer wall of collecting pipe body. The collecting pipe can hold sample volume 2mL, and the collecting pipe body passes through threaded connection in sampling pipe, and the mouth of pipe of collecting pipe body is greater than the liquid outlet diameter of filter head, breaks away from the sampling pipe below after the sampling, can seal and save. The scale may indicate the volume of sample entering the sampling tube. The volume of the fluid sample passing through the filter assembly is substantially constant and when the volume of saliva sample entering the sampling tube is 0.5-1mL, the volume of clarified saliva sample collected in the collection tube ranges between 0.15-0.3mL, thereby achieving collection of a relatively constant volume bodily fluid sample.

A body fluid collection, pretreatment and storage device, further comprising: the sampling funnel is movably connected with the sampling tube. The connected mode between sampling pipe and the sampling funnel includes: screw connection and gluing connection. Preferably, the sampling funnel is connected with the sampling pipe through threads, and the sampling funnel can be removed after sampling is finished.

The head of the push cylinder is made of rubber, the size of the push cylinder is the same as the inner diameter of the sampling tube, and the tail of the push cylinder is a handheld part. When the push cylinder is pushed into the sampling tube, the liquid at the bottom of the sampling tube and the air between the head of the push cylinder are compressed to generate 10-50N pressure, so that the liquid at the bottom of the sampling tube enters the collecting tube through the hydrophobic porous polymer filter plate and the filter head.

The biological sample protective agent is a reagent containing organic solvents and is separately stored in a well-sealed reagent bottle, and the organic solvents comprise: methanol, ethanol, acetonitrile, acetone, and a mixture of methanol and acetonitrile, preferably, the biological sample protectant is a mixture of methanol and acetonitrile, wherein the volume ratio of methanol to acetonitrile includes: 3:1,1:1,1:3.

Saliva samples were obtained using the apparatus described above in the following manner.

The saliva collecting, preprocessing and storing device comprises the following use steps:

step 1, a subject cleans an oral cavity and then calms to generate saliva for 2-5min, holds the upper end of a saliva sampling tube, spits the saliva into a sampling funnel, and the fluid enters the sampling tube through the sampling funnel and is intercepted on a porous high polymer filter plate at the bottom in the sampling tube, wherein the sampling volume is 0.5-1 mL;

step 2, separating the sampling funnel from the upper part of the sampling pipe, holding the tail part of the push cylinder by hand, directly pushing the head part of the push cylinder into the sampling pipe, compressing the gas in the sampling pipe, and allowing the body fluid in the sampling pipe main body to sequentially pass through the porous high polymer filter plate and the filter head of the filter assembly under the action of upper pressure and enter a collecting pipe below the sampling pipe;

step 3, separating the collecting pipe from the bottom of the sampling pipe to obtain a pretreated saliva sample with the volume of 0.15-0.3 mL;

and 4, pouring the biological sample protective agent into the collection pipe, covering the pipe cover tightly, and shaking forcibly to uniformly mix the protective agent and the saliva sample. Biological sample protectants include: methanol, ethanol, acetonitrile, acetone, a mixed solution of methanol and acetonitrile; the volume ratio of methanol to acetonitrile in the mixed solution of methanol and acetonitrile comprises: 3:1,1:1,1:3. The volume ratio of the biological sample protective agent to the fluid sample in the collection tube is 2:1-5: 1. The saliva sample containing the biological sample protective agent can be preserved at the temperature of 2-8 ℃ for more than one week, and the biological information of the sample is ensured not to be changed.

It should be noted that: the use method of the utility model can not influence the use of doctors after being protected, is an optimal operation method and does not belong to the scope of disease treatment methods.

The technical effect of the utility model is verified by the experiment;

the first experiment is an experiment for verifying the consistency improvement effect of a filter component in a saliva collecting device on a saliva sample;

1-1, adding a biological sample protective agent into a saliva sample collected by the device, storing the saliva sample at the temperature of 2-8 ℃ for 0-7 days, performing a centrifugation step, diluting the supernatant by 1-100 times, and performing subsequent molecular detection and analysis, wherein the result is shown in figure 2.

When the detected object is metabolic molecule, the centrifugal force is 2000-10000g, the supernatant diluent is the mixed solvent of methanol, acetonitrile and water, the detecting instrument is a mass spectrometer, and the detected molecular weight range is 20-350 Da.

The experimental results are shown in figure 3 as representative salivary metabolism profiles;

as can be seen from the figure: through the utility model discloses a saliva sample that body fluid collection, preliminary treatment and save set obtained contains a large amount of metabolism molecule information, can obtain through the mass spectrometric detection.

1-2, collecting saliva samples by using a bulk liquid collecting device and a collecting device which only uses a funnel and is matched with a collecting tube for three times by using the same individual sample to obtain a saliva metabolism spectrum and a Coefficient of Variation (CV) value thereof;

the experimental results are shown in figure 4.

And (4) analyzing results: as can be seen from the information of FIG. 4: the consistency of the saliva sample information obtained by the bulk liquid collecting device is obviously higher than that of the saliva sample information obtained by the conventional collecting device.

1-3, obtaining a metabolic spectrum after saliva samples collected by the same individual sample for three times pass through a hydrophilic porous polymer filter plate and a hydrophobic porous polymer filter plate;

the experimental results are shown in figure 5;

and (4) analyzing results: as can be seen from the figure: the consistency of the mass spectrum information of the metabolic sample after passing through the hydrophobic porous polymer filter plate is far better than that of the mass spectrum information of the metabolic sample after passing through the hydrophilic porous polymer filter plate.

The utility model discloses a mucin, cell piece, the insoluble substance in the body fluid sample such as saliva can be held back and adsorbed to porous high polymer filter, nevertheless can not arouse biological information's in the body fluid sample loss.

1-4, comparing the metabolic spectrogram of the saliva sample which only passes through a microporous filter membrane without using a porous high polymer filter plate;

the experimental results are shown in fig. 6;

and (4) analyzing results: as can be seen from fig. 6: the two have no obvious difference. But compare with the former, the utility model discloses a collection system is accomplishing filterable preliminary purification in-process, and the resistance is less, and easy operation is fit for non-professional person's operation.

The adsorption and filtration capacity of the filtering component to the viscous fluid sample is basically constant, and when the saliva sample volume entering the sampling tube is 0.5-1mL, the clear saliva sample volume range collected in the collecting tube is between 0.15-0.3mL, therefore, the utility model discloses a body fluid collecting device can realize the collection of the saliva sample with relatively stable volume.

The second experiment is to verify the storage condition experiment of the saliva sample obtained by adopting the device of the utility model;

the components in a human body fluid sample are complex, and blood, urine, saliva and the like all contain a large amount of protein, polypeptide, endogenous metabolites, inorganic ions and circulating free DNA. Saliva also contains a large amount of bacteria, cell debris, impurities and oral insolubles. The normal storage condition of the biological samples is ultralow temperature preservation, and the laboratory preservation temperature is-20 ℃ or-80 ℃. The utility model discloses a saliva collection system possesses the filtering component who has substep filtering action, can once only filter simultaneously and filter adhesive protein, cell piece, impurity, oral cavity insoluble and bacterium, consequently can reduce storage condition, more is applicable to non-centralized sampling.

To verify this effect, the specific operation steps of this embodiment are as follows:

step 1, a subject calmly produces saliva for 2-5min after cleaning the oral cavity, and continuously collects 3 tubes of saliva samples by using the body fluid sample collecting device;

step 2, dividing 3 parts of the collected 3 tubes of saliva samples respectively, and subpackaging the samples in equal volume;

step 3, storing the subpackaged saliva samples in refrigerators at 2-8 ℃, 20 ℃ and 80 ℃ for 72 hours respectively;

and 4, after 72 hours, carrying out metabolic molecule extraction experiments on the saliva samples under different storage conditions, carrying out mass spectrometry, and comparing spectrograms of the same sample under different storage conditions and calculating the coefficient of variation.

The experimental results are as follows: the 8 tubes of saliva samples were placed under 3 different storage conditions with the coefficient of variation after split charging, as shown in fig. 7;

and (4) analyzing results: as can be seen from fig. 7: the coefficient of variation of each tube of saliva sample is less than 20% under 3 different storage conditions, which indicates that the biomolecule information of the saliva sample can keep good consistency with the result under the storage conditions of-20 ℃ and-80 ℃ in a non-freezing state.

The utility model discloses a body fluid sample that saliva collection system gathered need not to carry out the low temperature and preserves.

Experiment three, verifying the non-low temperature stability of the saliva sample;

the experimental procedure is as follows:

step 1, a subject calmly produces saliva for 2-5min after cleaning the oral cavity, and continuously collects 3 tubes of saliva samples by using the body fluid sample collecting device;

step 2, dividing 7 parts of the collected 3 tubes of saliva samples respectively, and subpackaging the samples in equal volume;

step 3, adding the biological sample protective agent in the saliva sample collection device after subpackaging, fully oscillating uniformly, storing at 2-8 ℃, setting the storage time to be 0 hour, 4 hours, 8 hours, 24 hours, 72 hours (3 days), 120 hours (5 days), 168 hours (7 days), volatilizing the solvent of the supernatant after reaching the set time through a centrifugation step to form sample dry powder, and storing at-20 ℃ or-80 ℃;

and 4, re-dissolving the pretreated saliva sample dry powder at different time points, performing mass spectrometry, and comparing the spectrogram of the subpackaged sample which is placed on the same sample for 4 hours, 8 hours, 24 hours, 72 hours (3 days), 120 hours (5 days) and 168 hours (7 days) with the spectrogram of the sample which is placed on the sample for 0 hour and immediately processed, and calculating the coefficient of variation.

The experimental results are as follows: a representative spectrum of saliva samples at various times of exposure is shown in figure 8.

And (4) analyzing results: as can be seen from fig. 8: within 7 days, the coefficient of variation of saliva sample < 25%, show through the utility model discloses a saliva sample that saliva collection system gathered, preliminary treatment and preserved can keep the stability of longer time under the conventional family storage condition.

This device adopts the filter assembly of porous polymer filter cooperation filter, can guarantee to gather non-irritative saliva sample, again can once only eliminate mucin under the low resistance condition, the bacterium, the cell piece, the interference of oral cavity insoluble substance impurity, accomplish preliminary purification process, improve the biomolecule in the saliva sample, especially the stability of metabolism class molecule, especially hydrophobic porous polymer filter cooperation low adsorptivity microfiltration membrane effect is especially outstanding, such effect is that singly adopt hydrophobic porous polymer filter or singly adopt low adsorptivity microfiltration membrane effect that can not reach, the utility model discloses the creative discovery the two cooperation use can reach such unexpected effect.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by adopting equivalent replacement or equivalent transformation fall within the protection scope of the present invention.

Claims (8)

1. A body fluid collection, pretreatment and storage device comprising: the biological sample protective agent comprises a sampling tube, a push cylinder sliding in the sampling tube, a filter assembly arranged at the bottom of the sampling tube, a collecting tube connected with the filter assembly and a biological sample protective agent; the filter assembly includes: the porous high polymer filter plate is arranged in the sampling pipe and is connected with the filter head at the lower end of the sampling pipe.

2. The device for collecting, pretreating and storing a body fluid according to claim 1, wherein said porous polymer filter plate is a hydrophobic porous polymer filter plate having a planar size equal to the inner diameter of the sampling tube, a thickness of 1 to 3mm and a pore size of 10 to 100 μm.

3. A body fluid collection, pretreatment and preservation device according to claim 1, wherein said filter head comprises: the filter head body is arranged on the low-adsorption microporous filter membrane in the filter head body; the pore diameter of the low-adsorption microporous filter membrane is 0.2-0.5 mu m.

4. A body fluid collection, pretreatment and storage device according to claim 1, wherein said collection tube comprises: the collecting pipe comprises a collecting pipe body, a sharp bottom formed at the bottom of the collecting pipe body, a pipe cover connected to the collecting pipe body and scales formed on the outer wall of the collecting pipe body.

5. A body fluid collection, pretreatment and storage device according to claim 4, wherein said collection tube body is threadably attached to said sampling tube.

6. A body fluid collection, pretreatment and storage device according to claim 4, wherein the orifice of said collection tube body is larger than the diameter of the outlet of said filter head.

7. A body fluid collection, pretreatment and storage device according to claim 1, further comprising: the sampling funnel is movably connected with the sampling tube.

8. The device of claim 7, wherein the connection between the sampling tube and the sampling funnel comprises: screw connection and gluing connection.

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