CN212554211U

CN212554211U - Needle tube for punching agarose gel plate

Info

Publication number: CN212554211U
Application number: CN202020939850.9U
Authority: CN
Inventors: 许子涵; 杨卓霖
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-28
Filing date: 2020-05-28
Publication date: 2021-02-19
Anticipated expiration: 2030-05-28

Abstract

The utility model discloses a needle tube for punching an agarose gel plate, which relates to the technical field of electrophoresis experimental equipment and comprises a punching tube; the negative pressure assembly comprises a piston which can move up and down in the perforating pipe; the punching gun head comprises a gun head body on the lower portion of the punching gun head and a reducing pipe body on the upper portion of the punching gun head, the pipe diameter of the reducing pipe body is gradually reduced from bottom to top, the reducing pipe body comprises a large head end and a small head end, the small head end of the reducing pipe body is inserted into the punching pipe, the reducing pipe body is clamped on the punching pipe, and a containing cavity gap is formed between the outer wall of the reducing pipe body and the inner wall of the punching pipe. The utility model discloses can effectively punch to the agarose gel board, can avoid the rifle head that punches absorbent agarose after punching to drop.

Description

Needle tube for punching agarose gel plate

Technical Field

The utility model relates to an electrophoresis experimental facilities technical field, in particular to a needle tubing that is used for agarose gel board to punch.

Background

Gel electrophoresis is one of the conventional experiments in the field of biological research, researchers can directly know information such as nucleic acid purity, fragment size and the like through electrophoresis experiments, and in the experiment operation, holes need to be formed in a solidified agarose or polyacrylamide gel agarose gel plate, and a sample to be detected is placed in the punched holes.

The commonly used perforation methods mainly include a glass tube perforation method, a comb perforation method and a needle tube perforation method. The glass tube punching method comprises the steps of placing a solidified agarose gel plate on a punching template drawing, punching the agarose gel plate by using a hollow glass tube, and finally picking out agarose in a hole. The comb punching method is characterized in that a comb is inserted into agarose gel before the agarose gel is coagulated, and holes are formed by pulling out the comb after the agarose gel is coagulated. The needle punching method is that a needle is inserted into an agarose gel plate, then negative pressure is formed in the needle, the agarose is sucked up, and then the needle is removed and the agarose is blown out.

At the realization the utility model discloses in-process, the inventor finds to have following problem at least among the prior art, and at present, the needle tubing method of punching can solve the problem that glass pipe method of punching, comb method of punching appear betterly, but, the other problem appears, for example, the agarose of needle tubing suction drops easily, influences the effect of punching.

Disclosure of Invention

The utility model discloses aim at solving one of the above-mentioned technical problem among the prior art to a certain extent at least. Therefore, the embodiment of the utility model provides a needle tubing for agarose gel board punches can avoid the rifle head that punches to drop at the agarose that punches back suction.

The needle tube for punching the agarose gel plate according to the embodiment of the utility model comprises a punching tube; the negative pressure assembly comprises a piston which can move up and down in the perforating pipe; the punching gun head comprises a gun head body at the lower part and a reducing pipe body at the upper part, the pipe diameter of the reducing pipe body is gradually reduced from bottom to top, the reducing pipe body comprises a large head end and a small head end, the small head end of the reducing pipe body is inserted into the punching pipe so that the reducing pipe body is clamped on the punching pipe, and a containing cavity gap is formed between the outer wall of the reducing pipe body and the inner wall of the punching pipe.

In an optional or preferred embodiment, the negative pressure assembly further comprises a piston rod, the lower end of the piston rod is connected with the piston, the upper end of the piston rod is connected with a piston handle after passing through the perforating pipe, and the upper end of the perforating pipe is provided with a through hole for the piston rod to pass through.

In an alternative or preferred embodiment, the piston is a rubber member.

In an alternative or preferred embodiment, a thin film is arranged between the reducing pipe body and the perforated pipe.

In an alternative or preferred embodiment, the membrane is one of a latex membrane or a rubber membrane.

In an optional or preferred embodiment, the outer wall of the perforating pipe is provided with a first pressing portion, and the lance head pipe body is provided with a second pressing portion.

In an alternative or preferred embodiment, the lance tip body is in the shape of a circular tube having a height greater than 4 mm.

Based on the technical scheme, the embodiment of the utility model provides a following beneficial effect has at least: above-mentioned technical scheme, through the design rifle head that punches, behind the perforating pipe at reducing body chucking, reducing body outer wall and perforating pipe inner wall have between and hold the lacuna, when the rifle head downstream that punches, the rifle head body pierces through the agarose gel board to form cylindrical hole on the agarose gel board, then piston among the negative pressure component is at the intraductal rebound that punches, aspirate the agarose of cylindrical hole, make the interior agarose of rifle head body can flow into under the effect of suction and hold the lacuna, prevent that the agarose from dropping. The utility model discloses can effectively punch to the agarose gel board, can avoid the rifle head that punches absorbent agarose after punching to drop.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures represent only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from these figures without inventive effort.

Fig. 1 is a cross-sectional view of an embodiment of the present invention.

Detailed Description

This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.

In the description of the present invention, it should be understood that the positional or orientational descriptions, such as the central, longitudinal, lateral, length, width, thickness, upper, lower, front, rear, left, right, vertical, horizontal, top, bottom, inner, outer, clockwise, counterclockwise, axial, radial, circumferential, etc., are based on the positional or orientational relationships shown in the drawings and are for convenience of description only and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be considered as limiting the present invention.

In the description of the present invention, the meaning of a plurality is one or more, the meaning of a plurality is two or more, and larger, smaller, larger, etc. are understood as excluding the present number, and larger, lower, inner, etc. are understood as including the present number unless otherwise specifically limited. Furthermore, the descriptions of first and second are only for the purpose of distinguishing between technical features, and are not to be construed as indicating or implying relative importance or implying any number or order of indicated technical features.

In the description of the present invention, unless otherwise clear and definite limitations, words such as setting, arranging, installing, connecting, linking, fixing, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in the present invention by combining the specific contents of the technical solutions.

In the description of the present invention, unless otherwise expressly limited, the first feature may be located above or below the second feature and may be directly in contact with the second feature or indirectly in contact with the first feature through intervening media. Also, a first feature may be directly above or obliquely above a second feature, or merely that the first feature is at a higher level than the second feature. A first feature may be directly below or obliquely below a second feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1, a needle cannula for perforating agarose gel plates comprises a perforating tube 14, a negative pressure assembly and a perforating gun head, wherein the negative pressure assembly comprises a piston 22 which can move up and down in the perforating tube 14. Preferably, the negative pressure assembly further comprises a piston rod 21, the lower end of the piston rod 21 is connected with a piston 22, the upper end of the piston rod 21 penetrates through the perforating pipe 14 and then is connected with a piston handle 23, and the upper end of the perforating pipe 14 is provided with a through hole for the piston rod 21 to penetrate through. Specifically, the piston 22 is a rubber member.

The perforating gun head comprises a gun head pipe body 11 at the lower part and a reducing pipe body 13 at the upper part, the pipe diameter of the reducing pipe body 13 is gradually reduced from bottom to top, the reducing pipe body 13 comprises a large head end 121 and a small head end 122, and the small head end 122 of the reducing pipe body 13 is inserted into the perforating pipe 14 so that the reducing pipe body 13 is clamped on the perforating pipe 14. When the reducing pipe body 13 is clamped on the punching pipe 14, the first pressing part 16 is arranged on the outer wall of the punching pipe 14, and the second pressing part 12 is arranged on the gun head pipe body 11.

In addition, be provided with the film between reducing body 13 and the perforated pipe 14, the film is one of latex film or rubber film, the film of reducing body 13 lateral wall, the main cladding of film is in the one end of being close to microcephaly end 122, behind perforated pipe 14 with reducing body 13 chucking, the film can increase the frictional force between rifle head and the perforated pipe 14 of punching, prevent that the rifle head that punches from dropping, it is airtight also to make between rifle head and the perforated pipe 14 of punching, when preventing to suction agarose, in the external air got into perforated pipe 14 from the gap between rifle head and the perforated pipe 14 of punching, influence the suction effect.

As shown in fig. 1, an accommodating cavity 15 is formed between the outer wall of the reducing pipe body 13 and the inner wall of the perforating pipe 14, when the perforating gun head moves downwards, the gun head pipe body 11 pierces the agarose gel plate, the gun head pipe body 11 is in a circular pipe shape, and a cylindrical hole is formed in the agarose gel plate, and considering the thickness of the current agarose gel plate on the market, the height of the gun head pipe body 11 is larger than 4mm, so that the gun head pipe body 11 can be ensured to completely pierce the agarose gel plate; then, the piston 22 in the negative pressure assembly moves upwards in the perforating tube 14 to suck the agarose in the cylindrical hole, so that the agarose in the gun head tube body 11 can flow into the accommodating cavity 15 under the action of suction force to prevent the agarose from falling.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims

1. A needle tubing for perforating agarose gel plates, characterized in that: comprises that

A perforated pipe (14);

a negative pressure assembly comprising a piston (22) movable up and down within the perforated pipe (14); and

the punching gun head comprises a gun head body (11) at the lower part and a reducing pipe body (13) at the upper part, the pipe diameter of the reducing pipe body (13) is gradually reduced from bottom to top, the reducing pipe body (13) comprises a big head end (121) and a small head end (122), the small head end (122) of the reducing pipe body (13) is inserted into the punching pipe (14) so that the reducing pipe body (13) is clamped on the punching pipe (14), and an accommodating cavity gap (15) is formed between the outer wall of the reducing pipe body (13) and the inner wall of the punching pipe (14).

2. The needle cannula for agarose gel slab perforation according to claim 1, wherein: the negative pressure assembly further comprises a piston rod (21), the lower end of the piston rod (21) is connected with the piston (22), the upper end of the piston rod (21) penetrates through the punching pipe (14) and then is connected with a piston handle (23), and a through hole for the piston rod (21) to penetrate is formed in the upper end of the punching pipe (14).

3. The needle cannula for perforating agarose gel plates of claim 2, wherein: the piston (22) is a rubber member.

4. The needle cannula for agarose gel slab perforation according to claim 1, wherein: and a thin film is arranged between the reducing pipe body (13) and the punching pipe (14).

5. The needle cannula for agarose gel slab perforation according to claim 4, wherein: the film is one of a latex film or a rubber film.

6. The needle cannula for perforating agarose gel plates of any of claims 1 to 5, wherein: the outer wall of the perforating pipe (14) is provided with a first pressing part (16), and the gun head pipe body (11) is provided with a second pressing part (12).

7. The needle cannula for perforating agarose gel plates of any of claims 1 to 5, wherein: the gun head pipe body (11) is in a circular pipe shape, and the height of the gun head pipe body is larger than 4 mm.