CN212331271U

CN212331271U - Assembled electrophoresis experiment agarose gel board perforating device

Info

Publication number: CN212331271U
Application number: CN202020939808.7U
Authority: CN
Inventors: 许子涵; 杨卓霖
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-28
Filing date: 2020-05-28
Publication date: 2021-01-12
Anticipated expiration: 2030-05-28

Abstract

The utility model discloses an assembled electrophoresis experiment agarose gel plate perforating device, which comprises a bracket, wherein the bracket comprises a top plate, a movable plate, a supporting plate and a bottom plate which are sequentially arranged from top to bottom, the bottom plate is provided with a placing surface for placing the agarose gel plate, a plurality of first elastic pieces are arranged between the top plate and the movable plate, and a plurality of second elastic pieces are arranged between the bottom plate and the supporting plate; the negative pressure assembly comprises a piston capable of moving up and down in the needle tube and piston rods with the lower ends connected with the piston, the upper ends of the piston rods penetrate through the needle tube and then are fixed with the movable plate, through holes for the piston rods to penetrate through are formed in the upper ends of the needle tubes, and the needle tubes are fixed with the supporting plate. The utility model discloses can satisfy the actual requirement according to the different experiments, beat required hole fast.

Description

Assembled electrophoresis experiment agarose gel board perforating device

Technical Field

The utility model relates to an experimental facilities technical field, in particular to assembled electrophoresis experiment agarose gel board perforating device.

Background

Gel electrophoresis is one of the conventional experiments in the field of biological research, researchers can directly know information such as nucleic acid purity, fragment size and the like through electrophoresis experiments, and in the experiment operation, holes need to be formed in a solidified agarose or polyacrylamide gel agarose gel plate, and a sample to be detected is placed in the punched holes.

The commonly used perforation methods mainly include a glass tube perforation method and a comb perforation method. In realizing the utility model discloses the in-process, the utility model discloses there is the following problem at least among the people discovery prior art, glass manages the punching method earlier with the agarose gel board that has solidified put on the template drawing that punches, and the hollow glass pipe of reuse punches at the agarose gel board, picks out downthehole agarose at last, but, when glass pipe inserts the agarose gel board crooked when punching, can influence experiment judged result, after glass manages to punch, still need pick out downthehole agarose, waste time and energy very much. The comb punching method is characterized in that a comb is inserted into agarose gel before the agarose gel is coagulated, and holes are formed by pulling out the comb after the agarose gel is coagulated. Therefore, a device is needed for punching the agarose gel plate, which can adjust the number of holes according to the actual needs of the experiment and suck away the agarose in the holes.

Disclosure of Invention

The utility model discloses aim at solving one of the above-mentioned technical problem among the prior art to a certain extent at least. Therefore, the embodiment of the utility model provides an assembled electrophoresis experiment agarose gel board perforating device can satisfy the actual requirement of different experiments, beats out required hole fast to siphon away the agarose of hole.

The assembled electrophoresis experiment agarose gel plate perforating device comprises a support, wherein the support comprises a top plate, a movable plate, a supporting plate and a bottom plate which are sequentially arranged from top to bottom, the bottom plate is provided with a placing surface for placing the agarose gel plate, a plurality of first elastic pieces are arranged between the top plate and the movable plate, and a plurality of second elastic pieces are arranged between the bottom plate and the supporting plate; the negative pressure assembly comprises a piston capable of moving up and down in the needle tube and piston rods with the lower ends connected with the piston, the upper ends of the piston rods penetrate through the needle tube and then are fixed with the movable plate, through holes for the piston rods to penetrate through are formed in the upper ends of the needle tubes, and the needle tubes are fixed with the supporting plate.

In an alternative or preferred embodiment, the piston rod is connected with a piston handle at the upper end, the movable plate is provided with a first groove for horizontally sliding each piston handle, the needle tube is provided with an annular flange, and the supporting plate is provided with a second groove for horizontally sliding the annular flange.

In an optional or preferred embodiment, the first groove and the second groove are both provided with an entrance, and the entrance is provided with a locking switch.

In an alternative or preferred embodiment, the support plate and/or the movable plate are marked with a scale.

In an alternative or preferred embodiment, the first elastic member is a tension spring, and the second elastic member is a compression spring.

In an alternative or preferred embodiment, the upper end of the second elastic member is connected to the supporting plate, and the bottom plate is provided with a base for connecting the lower end of the second elastic member.

In an alternative or preferred embodiment, the base is provided with a guide post, and the second elastic member is sleeved on the guide post.

Based on the technical scheme, the embodiment of the utility model provides a following beneficial effect has at least: according to the technical scheme, the supporting plate is pressed downwards rapidly, the second elastic piece is compressed, the needle tube fixed on the supporting plate moves downwards rapidly along with the supporting plate, air above the piston cannot be discharged timely due to the fact that a gap between the piston rod and a through hole in the upper end of the needle tube is small, pressure above the piston is increased, the piston can move upwards slowly relative to the needle tube, at the moment, the piston rod drives the movable plate to move downwards, and the movable plate drives the first elastic piece to stretch; when the bottom end of the needle tube completely pierces the agarose gel plate to be punched, a hole with the same size as the bottom end of the needle tube is formed in the agarose gel plate, the height of the supporting plate is kept unchanged, air above the piston flows out from a gap between the piston rod and the through hole at the upper end of the needle tube, the pressure above the piston and in the needle tube is reduced, the piston rod in the movable plate is moved upwards by the stretched first elastic piece, negative pressure is formed below the piston, and agarose in the bottom end of the needle tube is sucked into the needle tube; when no agarose is at the bottom end of the needle tube, the pressing of the supporting plate is stopped, and the compressed second elastic piece enables the supporting plate to ascend and restore to the original position, so that a hole with the same size as the bottom end of the needle tube is punched on the agarose gel plate, and the operation of the whole process is simple. The utility model discloses a press the backup pad, can accomplish punching of a plurality of needle tubes to the agarose gel board, the time node of each needle tube negative pressure that forms is unified, can beat a plurality of holes fast, and, the operator can be according to the actual need adjustment needle tube's of experiment quantity, if the piston rod upper end is connected with the piston handle, the fly leaf is equipped with the first groove that supplies piston handle horizontal slip, the needle tube is provided with the annular flange, the backup pad is equipped with the second groove that supplies annular flange horizontal slip, still interval between the horizontal position of adjustable needle tube and each needle tube, thereby satisfy the actual requirement according to different experiments, beat required hole.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures represent only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from these figures without inventive effort.

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is a cross-sectional view taken along line A-A of FIG. 1;

fig. 3 is a schematic structural diagram of the support plate in the embodiment of the present invention.

Detailed Description

This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.

In the description of the present invention, it should be understood that the positional or orientational descriptions, such as the central, longitudinal, lateral, length, width, thickness, upper, lower, front, rear, left, right, vertical, horizontal, top, bottom, inner, outer, clockwise, counterclockwise, axial, radial, circumferential, etc., are based on the positional or orientational relationships shown in the drawings and are for convenience of description only and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be considered as limiting the present invention.

In the description of the present invention, the meaning of a plurality is one or more, the meaning of a plurality is two or more, and larger, smaller, larger, etc. are understood as excluding the present number, and larger, lower, inner, etc. are understood as including the present number unless otherwise specifically limited. Furthermore, the descriptions of first and second are only for the purpose of distinguishing between technical features, and are not to be construed as indicating or implying relative importance or implying any number or order of indicated technical features.

In the description of the present invention, unless otherwise clear and definite limitations, words such as setting, arranging, installing, connecting, linking, fixing, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in the present invention by combining the specific contents of the technical solutions.

In the description of the present invention, unless otherwise expressly limited, the first feature may be located above or below the second feature and may be directly in contact with the second feature or indirectly in contact with the first feature through intervening media. Also, a first feature may be directly above or obliquely above a second feature, or merely that the first feature is at a higher level than the second feature. A first feature may be directly below or obliquely below a second feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1 to 3, an assembled electrophoresis experiment agarose gel plate perforating device includes a frame and a plurality of needle tubes 10.

As shown in fig. 1 and 2, the bracket includes a top plate 61, a movable plate 51, a support plate 41 and a bottom plate 31 sequentially arranged from top to bottom, the bottom plate 31 has a placing surface for placing the sepharose plate 100, a plurality of first elastic members 53 are arranged between the top plate 61 and the movable plate 51, and a plurality of second elastic members 46 are arranged between the bottom plate 31 and the support plate 41. Specifically, the first elastic member 53 is a tension spring, and the second elastic member 46 is a compression spring. Wherein, the upper end of the second elastic member 46 is connected to the supporting plate 41, and the bottom plate 31 is provided with a base 32 for connecting the lower ends of the corresponding second elastic members 46. The base 32 is provided with guide posts 45, and the second elastic member 46 is sleeved on the guide posts 45, it can be understood that the support plate 41 moves along the direction of each guide post 45 during the downward movement process, of course, in some embodiments, the support plate 41 is provided with a guide hole for the guide post 45 to pass through, or the base 32 is provided with a guide hole for the guide post 45 to pass through. In the present embodiment, two bases 32 are provided, and of course, the number of the bases 32 can be increased according to the size of the supporting plate 41.

Specifically, as shown in fig. 2, the needle tube 10 includes a perforated tube 14 and a perforating gun head, the perforating gun head includes a gun head tube 11 on the lower portion and a reducing tube 13 on the upper portion, the diameter of the reducing tube 13 is gradually reduced from bottom to top, the reducing tube 13 includes a big end 131 and a small end 132, the small end 132 of the reducing tube 13 is inserted into the perforated tube 14 so that the reducing tube 13 is tightly clamped on the perforated tube 14, and an accommodating cavity gap 15 is formed between the outer wall of the reducing tube 13 and the inner wall of the perforated tube 14. The perforating gun head is made of smooth and hard materials, such as glass, stainless steel, metal and the like. The gun head tube body 11 is in a circular tube shape, a cylindrical hole is formed in the agarose gel plate, and the height of the gun head tube body 11 is larger than 4mm in consideration of the thickness of the current agarose gel plate on the market, so that the gun head tube body 11 can be ensured to completely pierce the agarose gel plate.

The needle tube 10 is provided therein with a negative pressure unit including a piston 22 movable up and down in the needle tube 10 and a piston rod 21 connected to the piston 22 at a lower end thereof, specifically, the piston 22 is a rubber member, and the piston 22 is located in the perforated tube 14. The upper end of each piston rod 21 is fixed with the movable plate 51 after passing through the needle tube 10, the upper end of the needle tube 10 is provided with a through hole for the piston rod 21 to pass through, and the needle tube 10 is fixed with the support plate 41, in particular the perforated pipe 14 is fixed with the support plate 41. Wherein, the through hole is arranged at the upper end of the perforating pipe 14, and the through hole at the upper end of the perforating pipe 14 is slightly larger than the diameter of the piston rod 21.

Preferably, the piston rod 21 is connected with the piston handle 23 at the upper end thereof, the movable plate 51 has a first groove 52 for horizontally sliding each piston handle 23, the needle tube 10 is provided with the annular flange 17, and the support plate 41 has a second groove 42 for horizontally sliding the annular flange 17. Further, the first slot 52 and the second slot 42 are opened with an entrance 43, the entrance 43 is provided with a locking switch 44, wherein fig. 3 shows the structure of the supporting plate 41, and the structure of the movable plate 51 can refer to the supporting plate 41. It will be appreciated that each needle cannula 10 may be mounted to the holder through the access opening 43 and that the needle cannula 10 may be moved horizontally through the corresponding first slot 52 and second slot 42 to adjust the spacing therebetween to meet different perforation requirements. Further, the supporting plate 41 and/or the movable plate 51 are marked with scales, and the operator can measure the distance between two connected needle cannulae 10. And the distance between two adjacent needle tubes 10 is adjusted according to actual needs, so that the needle tubes 10 punch holes with equal distance on the agarose gel plate 100.

Furthermore, taking the supporting plate 41 as an example, one end of the locking switch 44 is hinged to the supporting plate 41, and the other end is clipped into the other side of the insertion opening 43, in other embodiments, the locking switch 44 may be in the form of a tab, and the supporting plate 41 is provided with a corresponding slot at the position of the insertion opening 43. The structure of the movable plate 51 may be set with reference to the support plate 41.

The specific operation is as follows:

s1, pressing the support plate 41 downwards quickly, the second elastic element 46 sleeved on the guide post 45 is compressed, the needle tube 10 fixed on the support plate 41 moves downwards quickly along with the support plate 41, because the gap between the piston rod 21 and the through hole at the upper end of the perforated tube 14 is small, air above the piston 22 cannot be discharged in time, the pressure above the piston 22 increases, the piston 22 can only move upwards slowly relative to the needle tube 10, and of course, in other embodiments, the friction between the piston and the perforated tube can also make the piston move upwards slowly relative to the needle tube. At this time, the piston rod 21 drives the movable plate 51 to move downward, and the movable plate 51 drives each first elastic member 53 to stretch.

S2, when the gun head tube 11 completely pierces the agarose plate 100 to be punched, a hole with the same size as the gun head tube 11 is formed in the agarose plate 100, the height of the supporting plate 41 is kept unchanged, air above the piston 22 flows out from a gap between the piston rod 21 and the through hole at the upper end of the punching tube 14, the pressure above the piston 22 and in the punching tube 14 is reduced, the piston rod 21 in the movable plate is moved upwards by the stretched first elastic piece 53, negative pressure is formed below the piston, agarose in the gun head is sucked into the accommodating cavity 15 between the gun head and the punching tube 14, and the accommodating cavity 15 can prevent the sucked agarose from falling.

S3, when no agarose is left in the tip, the pressing of the support plate 41 is stopped, and the compressed second elastic member 45 lifts the support plate 41 to return to its original position, thereby punching a hole having the same size as the tip in the agarose plate 100.

The utility model discloses in, as shown in figure 2, needle tubing 10 includes perforated pipe 14 and the rifle head that punches, the operator can be according to actual need, change the rifle head that punches, as preferred, be provided with the film between reducing body 13 and the perforated pipe 14, the film is one of them kind of latex film or rubber film, the film of reducing body 13 lateral wall, the main cladding of film is close to tip 122 one end, with reducing body 13 chucking behind perforated pipe 14, the film can increase the frictional force between rifle head and the perforated pipe 14 that punches, prevent that the rifle head that punches from dropping, it is airtight also to make between rifle head and the perforated pipe 14 that punches, when preventing to suck the agarose, the gap gets into perforated pipe 14 between rifle head and the perforated pipe 14 from outside air, influence suction effect. In addition, when the reducing pipe body 13 is clamped on the punching pipe 14, the first pressing part 16 is arranged on the outer wall of the punching pipe 14, and the second pressing part 12 is arranged on the gun head pipe body 11.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims

1. The utility model provides an assembled electrophoresis experiment agarose gel board perforating device which characterized in that: comprises that

The support comprises a top plate (61), a movable plate (51), a supporting plate (41) and a bottom plate (31) which are sequentially arranged from top to bottom, wherein the bottom plate (31) is provided with a placing surface for placing an agarose gel plate (100), a plurality of first elastic pieces (53) are arranged between the top plate (61) and the movable plate (51), and a plurality of second elastic pieces (46) are arranged between the bottom plate (31) and the supporting plate (41); and

the negative pressure needle tube structure is characterized in that a plurality of needle tubes (10) are arranged in the needle tubes (10), each negative pressure component comprises a piston (22) capable of moving up and down in the needle tube (10) and a piston rod (21) with the lower end connected with the piston (22), the upper end of each piston rod (21) penetrates through the needle tube (10) and then is fixed with the movable plate (51), a through hole for the piston rod (21) to penetrate through is formed in the upper end of the needle tube (10), and the needle tube (10) is fixed with the supporting plate (41).

2. The assembled electrophoresis experiment agarose gel plate perforating device of claim 1, wherein: the upper end of the piston rod (21) is connected with a piston handle (23), the movable plate (51) is provided with a first groove (52) for the horizontal sliding of each piston handle (23), the needle tube (10) is provided with an annular flange (17), and the supporting plate (41) is provided with a second groove (42) for the horizontal sliding of the annular flange (17).

3. The assembled electrophoresis experiment agarose gel plate perforating device of claim 2, wherein: an inlet (43) is formed in each of the first groove (52) and the second groove (42), and a locking switch (44) is arranged at each inlet (43).

4. The assembled electrophoresis experiment agarose gel plate perforating device of claim 2, wherein: the support plate (41) and/or the movable plate (51) are marked with scales.

5. The assembled electrophoresis experiment agarose gel plate perforating device of any one of claims 1 to 4, wherein: the first elastic piece (53) is a tension spring, and the second elastic piece (46) is a compression spring.

6. The assembled electrophoresis experiment agarose gel plate perforating device of claim 5, wherein: the upper end of the second elastic piece (46) is connected with the supporting plate (41), and a base (32) for connecting the lower end of the second elastic piece (46) is installed on the bottom plate (31).

7. The assembled electrophoresis experiment agarose gel plate perforating device of claim 6, wherein: the base (32) is provided with a guide post (45), and the second elastic piece (46) is sleeved on the guide post (45).