CN212483602U - Parathyroid hormone quantitative determination kit - Google Patents
Parathyroid hormone quantitative determination kit Download PDFInfo
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- CN212483602U CN212483602U CN201922389521.1U CN201922389521U CN212483602U CN 212483602 U CN212483602 U CN 212483602U CN 201922389521 U CN201922389521 U CN 201922389521U CN 212483602 U CN212483602 U CN 212483602U
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Abstract
The utility model discloses a parathyroid hormone quantitative determination kit, the inner wall fixedly connected with constant temperature cystosepiment of shell, the inside fixed mounting of constant temperature cystosepiment has the kit, the bottom fixedly connected with support frame of kit, the inside fixed mounting of kit has reagent frame, the inside fixedly connected with reagent pipe of reagent frame, the bottom fixedly connected with bottom plate of kit, the bottom fixedly connected with support plate of support frame, the top fixedly connected with ice-cube of support plate, the inside fixedly connected with intubate of support plate, the inside fixedly connected with baffle of the bottom of shell, the inside fixedly connected with outlet pipe of baffle; the utility model discloses the kit is when carrying out quantitative inspection to the parathyroid hormone, and reagent is in microthermal state, has improved the rate of accuracy that detects, has also solved the problem of placing easy inefficacy under the reagent normal atmospheric temperature simultaneously.
Description
Technical Field
The utility model relates to a kit technical field specifically is a parathyroid hormone quantitative determination kit.
Background
Parathyroid hormone is an alkaline single-chain polypeptide hormone secreted by parathyroid chief cells, and has the main functions of regulating the metabolism of calcium and phosphorus in vertebrates, promoting the increase of calcium level in blood and the decrease of phosphorus level in blood, PTH promoting the increase of calcium ion concentration in plasma, and the main target organs of the action are bones and kidneys, which mobilize bone calcium into blood, promote the reabsorption of calcium ion and the excretion of phosphate by renal tubules, so that the calcium concentration in blood is increased and the phosphorus concentration in blood is decreased.
The kit is usually used for boxes for containing chemical reagents for detecting chemical components, drug residues, virus types and the like, and is generally used in hospitals and pharmaceutical enterprises, but the existing kit is a common box and cannot cool the parathyroid hormone, and the chemiluminescence diagnostic reagent is often used for multiple times after being unsealed and is easy to lose efficacy when being placed at normal temperature, so that the parathyroid hormone quantitative detection kit is provided to solve the problems.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model provides a parathyroid hormone quantitative determination kit has improved the rate of accuracy that detects, has also solved the problem of placing easy inefficacy under the reagent normal atmospheric temperature simultaneously.
In order to achieve the above object, the utility model provides a following technical scheme: a parathyroid hormone quantitative determination kit comprises a shell, wherein the inner wall of the shell is fixedly connected with a constant temperature foam board, the constant temperature foam board is internally and fixedly provided with a reagent box, the bottom of the reagent box is fixedly connected with a supporting frame, a reagent rack is fixedly arranged in the reagent kit, a reagent tube is fixedly connected in the reagent rack, the bottom of the kit is fixedly connected with a bottom plate, the bottom of the support frame is fixedly connected with a support plate, the top of the carrier plate is fixedly connected with an ice block, the inner part of the carrier plate is fixedly connected with an insertion tube, the inner bottom of the shell is fixedly connected with a baffle plate, the interior of the baffle is fixedly connected with a water outlet pipe, the bottom of the shell is fixedly connected with a base, the front of shell fixedly connected with label, the top fixedly connected with sealed roof of shell, the top fixedly connected with handle of sealed roof.
Preferably, the reagent tubes include a first reagent tube, a second reagent tube, a third reagent tube, a fourth reagent tube and a fifth reagent tube, the first reagent tube, the second reagent tube, the third reagent tube, the fourth reagent tube and the fifth reagent tube are sequentially arranged, the first reagent tube is a carboxyl magnetic bead coupled parathyroid hormone monoclonal antibody solution, the second reagent tube is an acridinium ester labeled parathyroid hormone monoclonal antibody solution, the third reagent tube is a parathyroid hormone solution with the concentration of 500ng/L, the fourth reagent tube is a chemiluminescent pre-excitation liquid A, and the fifth reagent tube is a chemiluminescent pre-excitation liquid B.
Preferably, the chemiluminescence pre-excitation liquid A consists of H2O2 and HNO3, the mass fraction of H2O2 is 1.5%, and the concentration of HNO3 is 0.1 mol/L.
Preferably, the chemiluminescence excitation liquid B consists of Triton X-100 and NaOH, the mass fraction of the Triton X-100 is 1.0%, and the concentration of the NaOH is 0.4 mol/L.
Preferably, the bottom plate is internally provided with air holes, the top of the bottom plate is provided with a placing groove, and the inside of the placing groove is fixedly connected with the reagent tube.
Preferably, a slot is formed in the carrier plate, and an insertion tube is fixedly connected in the slot.
Preferably, the baffle is arc-shaped, and the left side and the right side of the baffle are fixedly connected with the inner walls of the left side and the right side of the shell respectively.
Preferably, the inside joint of outlet pipe has the card stopper, the outlet pipe extends to the outside of shell through the inside of shell.
Preferably, thread grooves are formed in the left side and the right side of the inside of the sealing top plate, thread pieces are fixedly connected to the left side and the right side of the shell, and the thread grooves are in threaded connection with the thread pieces.
The utility model discloses possess following beneficial effect: this parathyroid hormone quantitative determination kit, through setting up the shell, the constant temperature cystosepiment, the kit, the support frame, the reagent frame, reagent pipe and ice-cube, thereby make ice-cube self air conditioning of effluvium can be to diffusion all around, and seal the mouth through sealed roof, through the isolated temperature of constant temperature cystosepiment, thereby guarantee that the inside temperature of kit is unanimous and be in the low temperature, and the ice-cube can leak down through the intubate after melting, block through the baffle, the outlet pipe discharges, when carrying out quantitative inspection to parathyroid hormone, reagent is in microthermal state, the rate of accuracy that detects has been improved, the problem of placing easy inefficacy under the reagent normal atmospheric temperature has also.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a front view of the present invention.
In the figure: the kit comprises a shell 1, a constant-temperature foam plate 2, a kit 3, a support frame 4, a reagent frame 5, a reagent tube 6, a bottom plate 7, a support plate 8, an ice block 9, an insertion tube 10, a baffle plate 11, a water outlet tube 12, a base 13, a label 14, a sealed top plate 15 and a handle 16.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
Referring to fig. 1-2, a parathyroid hormone quantitative detection kit comprises a housing 1, a constant temperature foam board 2 is fixedly connected to the inner wall of the housing 1, a kit 3 is fixedly installed inside the constant temperature foam board 2, a support frame 4 is fixedly connected to the bottom of the kit 3, a reagent frame 5 is fixedly installed inside the kit 3, a reagent tube 6 is fixedly connected inside the reagent frame 5, a bottom plate 7 is fixedly connected to the bottom of the kit 3, air holes are formed inside the bottom plate 7, a placement groove is formed in the top of the bottom plate 7, the interior of the placement groove is fixedly connected to the reagent tube 6, a support plate 8 is fixedly connected to the bottom of the support frame 4, an ice block 9 is fixedly connected to the top of the support plate 8, an insertion tube 10 is fixedly connected to the interior of the support plate 8, an insertion slot is formed in the interior of the support plate 8, the baffle 11 is arc-shaped, the left side and the right side of the baffle 11 are respectively fixedly connected with the inner walls of the left side and the right side of the shell 1, the inner part of the baffle 11 is fixedly connected with a water outlet pipe 12, the inner part of the water outlet pipe 12 is clamped with a clamping plug, the water outlet pipe 12 extends to the outer part of the shell 1 through the inner part of the shell 1, the bottom of the shell 1 is fixedly connected with a base 13, the front of the shell 1 is fixedly connected with a label 14, the top of the shell 1 is fixedly connected with a sealing top plate 15, the left side and the right side of the inner part of the sealing top plate 15 are both provided with thread grooves, the left side and the right side of the shell 1 are both fixedly connected with thread sheets, the thread grooves are in thread connection with the thread sheets, the top of the sealing top plate 15 is fixedly connected with a handle 16, and by arranging the shell 1, the, and through sealed roof 15 the mouth is sealed up, through the isolated temperature of constant temperature cystosepiment 2 to guarantee that the inside temperature of kit 3 is unanimous at the low temperature, and ice-cube 9 can let down through intubate 10 after melting, blocks through baffle 11, and outlet pipe 12 discharges, thereby when carrying out quantitative inspection to reagent, the temperature that reagent was located is at the low temperature forever, can not infect, thereby has improved the effect of depositing.
In conclusion, this parathyroid hormone quantitative determination kit, through setting up shell 1, constant temperature cystosepiment 2, kit 3, support frame 4, reagent frame 5, reagent pipe 6 and ice-cube 9, thereby make the cold air that ice-cube 9 self effused to spread all around, and seal up the mouth through sealed roof 15, through the isolated temperature of constant temperature cystosepiment 2, thereby guarantee that the inside temperature of kit 3 is unanimous in the low temperature, and ice-cube 9 can leak down through intubate 10 after melting, block through baffle 11, outlet pipe 12 discharges, when carrying out quantitative inspection to parathyroid hormone, the reagent is in microthermal state, the rate of accuracy of detection has been improved, the problem of placing the easy inefficacy under the reagent normal temperature has also been solved simultaneously.
The reagent tube comprises a first reagent tube, a second reagent tube, a third reagent tube, a fourth reagent tube and a fifth reagent tube which are sequentially arranged, wherein a carboxyl magnetic bead coupled parathyroid hormone monoclonal antibody solution is arranged in the first reagent tube, a parathyroid hormone monoclonal antibody solution marked by acridinium ester is arranged in the second reagent tube, a parathyroid hormone solution with the concentration of 500ng/L is arranged in the third reagent tube, a chemiluminescence pre-excitation liquid A is arranged in the fourth reagent tube, and a chemiluminescence pre-excitation liquid B is arranged in the fifth reagent tube.
The chemiluminescence pre-excitation liquid A consists of H2O2 and HNO3, the mass fraction of H2O2 is 1.5%, and the concentration of HNO3 is 0.1 mol/L.
The chemiluminescence excitation liquid B consists of Triton X-100 and NaOH, wherein the mass fraction of the Triton X-100 is 1.0%, and the concentration of the NaOH is 0.4 mol/L.
Example 2
The detection method of the kit comprises the following steps: (1) adding 50 mu L of sample to be detected into the reaction cup, then adding 150 mu L of carboxyl magnetic bead coupled parathyroid hormone monoclonal antibody solution, oscillating and mixing uniformly, and incubating for 8min at 37 ℃. (2) Separating and washing for 3 times, and fully oscillating the washed reaction container to disperse the magnetic particles. (3) 150. mu.L of an acridinium ester-labeled parathyroid hormone monoclonal antibody solution was added to the reaction cup, shaken and mixed, and incubated at 37 ℃ for 7 min. (4) Separating, cleaning for 3 times, and oscillating the reaction vessel after cleaning to disperse the magnetic particles. (5) Adding 100 mu L of chemiluminescence pre-excitation liquid A, adding 100 mu L of chemiluminescence excitation liquid B after 1s, measuring the relative luminescence intensity, wherein the content of parathyroid hormone in the sample is in a certain proportion relation with the relative luminescence intensity.
The utility model discloses well parathyroid hormone monoclonal antibody is purchased from Yubo biology, and the carboxyl magnetic bead is purchased and is flown from the saimer, and acridine ester is purchased from Wuhan De Cheng biochemical science and technology ltd, and parathyroid hormone monoclonal antibody and carboxyl magnetic bead coupling adopt conventional method (EDC is the coupling agent), and acridine ester mark parathyroid hormone monoclonal antibody adopts conventional method (DMF is the solvent), and the washing liquid is purchased from Shanghai feather biology, and parathyroid hormone solution adopts 2% BSA's Tris-HCl buffer solution to dilute.
It is to be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A parathyroid hormone quantitative determination kit, includes shell (1), its characterized in that: the inner wall of the shell (1) is fixedly connected with a constant-temperature foam board (2), the inside of the constant-temperature foam board (2) is fixedly provided with a reagent box (3), the bottom of the reagent box (3) is fixedly connected with a support frame (4), the inside of the reagent box (3) is fixedly provided with a reagent frame (5), the inside of the reagent frame (5) is fixedly connected with a reagent tube (6), the bottom of the reagent box (3) is fixedly connected with a bottom plate (7), the bottom of the support frame (4) is fixedly connected with a support plate (8), the top of the support plate (8) is fixedly connected with an ice block (9), the inside of the support plate (8) is fixedly connected with an insertion tube (10), the inner bottom of the shell (1) is fixedly connected with a baffle plate (11), the inside of the baffle plate (11) is fixedly connected with an outlet pipe (12, the front surface of the shell (1) is fixedly connected with a label (14), the top of the shell (1) is fixedly connected with a sealing top plate (15), and the top of the sealing top plate (15) is fixedly connected with a handle (16); the reagent tube testing device is characterized in that air holes are formed in the bottom plate (7), a placing groove is formed in the top of the bottom plate (7), and the inside of the placing groove is fixedly connected with the reagent tube (6).
2. The kit for quantitatively detecting parathyroid hormone according to claim 1, wherein: the baffle (11) is arc-shaped, and the left side and the right side of the baffle (11) are fixedly connected with the inner walls of the left side and the right side of the shell (1) respectively.
3. The kit for quantitatively detecting parathyroid hormone according to claim 1, wherein: the inside joint of outlet pipe (12) has the jam, outlet pipe (12) extend to the outside of shell (1) through the inside of shell (1).
4. The kit for quantitatively detecting parathyroid hormone according to claim 1, wherein: thread grooves are formed in the left side and the right side of the inside of the sealing top plate (15), thread sheets are fixedly connected to the left side and the right side of the shell (1), and the thread grooves are in threaded connection with the thread sheets.
5. The kit for quantitatively detecting parathyroid hormone according to claim 1, wherein: the reagent tube comprises a first reagent tube, a second reagent tube, a third reagent tube, a fourth reagent tube and a fifth reagent tube which are sequentially arranged, wherein a carboxyl magnetic bead coupled parathyroid hormone monoclonal antibody solution is arranged in the first reagent tube, a parathyroid hormone monoclonal antibody solution marked by acridinium ester is arranged in the second reagent tube, a parathyroid hormone solution with the concentration of 500ng/L is arranged in the third reagent tube, a chemiluminescence pre-excitation liquid A is arranged in the fourth reagent tube, and a chemiluminescence pre-excitation liquid B is arranged in the fifth reagent tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922389521.1U CN212483602U (en) | 2019-12-26 | 2019-12-26 | Parathyroid hormone quantitative determination kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922389521.1U CN212483602U (en) | 2019-12-26 | 2019-12-26 | Parathyroid hormone quantitative determination kit |
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| CN212483602U true CN212483602U (en) | 2021-02-05 |
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| CN201922389521.1U Active CN212483602U (en) | 2019-12-26 | 2019-12-26 | Parathyroid hormone quantitative determination kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111103431A (en) * | 2019-12-26 | 2020-05-05 | 宁波奥丞生物科技有限公司 | Parathyroid hormone quantitative determination kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111103431A (en) * | 2019-12-26 | 2020-05-05 | 宁波奥丞生物科技有限公司 | Parathyroid hormone quantitative determination kit |
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