CN212451443U - Stirring type temperature control cell culture device - Google Patents
Stirring type temperature control cell culture device Download PDFInfo
- Publication number
- CN212451443U CN212451443U CN202020578323.XU CN202020578323U CN212451443U CN 212451443 U CN212451443 U CN 212451443U CN 202020578323 U CN202020578323 U CN 202020578323U CN 212451443 U CN212451443 U CN 212451443U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- cell culture
- shell
- controller
- circulating pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000003756 stirring Methods 0.000 title claims abstract description 29
- 238000004113 cell culture Methods 0.000 title claims abstract description 24
- 230000008878 coupling Effects 0.000 claims abstract description 13
- 238000010168 coupling process Methods 0.000 claims abstract description 13
- 238000005859 coupling reaction Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 230000008859 change Effects 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000013019 agitation Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000012007 large scale cell culture Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001012 protector Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a stirring type control by temperature change cell culture ware, which comprises an outer shell, the inside of shell is equipped with the culture dish, the inner chamber top rigid coupling temperature sensor and the agitating unit of culture dish, and the outside bottom of culture dish is equipped with the heating pipe, the bottom rigid coupling controller of shell, outside one side rigid coupling display screen of shell, the culture dish outside still is equipped with the circulating pump that is used for cooling the culture dish, and temperature sensor passes through the connecting wire with the input of controller and is connected, and the display screen, circulating pump and heating pipe pass through the wire with the output of controller and are connected. Through the utility model discloses the accurate control by temperature change adjusting device and agitating unit of device have realized that each regional temperature is balanced in the incubator, and the material distributes evenly, and the cell obtains the growth environment of optimizing, cultivates efficiency and is showing and improve. In addition, set up pulley and wheel in the bottom of cell culture ware and stop, can also fix at any time when can removing the culture ware, improved the utility model discloses the practicality of device.
Description
Technical Field
The utility model belongs to the field of cell biology, concretely relates to stirring type control by temperature change cell culture ware.
Background
The cell culture technique is also called cell cloning technique, and cell culture is an indispensable process for the whole biological engineering technique or one of the biological cloning techniques. Cell culture is the large-scale cloning of cells, a cell culture technology can be used for making simple single cells or multi-cells with few differentiation through mass culture of one cell, which is an essential link of the cloning technology, and a large number of cells or metabolites thereof can be obtained through cell culture, so the cell culture is the most core and basic technology in biotechnology. The prior large-scale cell culture technology widely uses a pot-shaped cell culture device, but the culture device generally adopts static culture, temperature, nutrient and oxygen cannot be uniformly distributed in each area in the pot, and the cell growth efficiency is greatly limited.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a stirring type control by temperature change cell culture ware can realize that the temperature of each region is balanced in the culture ware.
The utility model discloses the technical scheme who adopts is, a stirring type control by temperature change cell culture ware, which comprises an outer shell, the inside of shell is equipped with the culture dish, inner chamber top rigid coupling temperature sensor and agitating unit of culture dish, the outside bottom of culture dish is equipped with the heating pipe, the bottom rigid coupling controller of shell, outside one side rigid coupling display screen of shell, the culture dish outside still is equipped with the circulating pump that is used for cooling the culture dish, temperature sensor passes through the connecting wire with the input of controller and is connected, the display screen, circulating pump and heating pipe pass through the wire with the output of controller and are connected.
The utility model discloses technical scheme's that adopts characteristics still lie in, and the circulating pump setting is at the top of culture dish, and circulating pump intercommunication water pipe, the one end top-down of water pipe encircle the outer wall of culture dish and communicate with the circulating pump again.
The stirring device comprises a rotating motor, the rotating motor is connected with the output end of the controller, the rotating motor is fixedly connected to the top of the inner cavity of the culture dish, a stirring rod is embedded in a linkage shaft of the rotating motor, and a plurality of detachable stirring blades are fixedly connected to the side wall of the stirring rod.
The equal rigid coupling in inner chamber both sides of culture dish has the sterilamp, and every sterilamp all overcoat has the lamp shade, and the lamp shade passes through bolt threaded connection with the culture dish.
The outer wall of shell is embedded to have glass observation window, and observation window passes through bolt threaded connection with the shell.
The bottom fixed mounting of shell has a pair of support frame, and bolt threaded connection is passed through with the bottom of shell to every support frame, the bottom rigid coupling pulley of every support frame, and one side rigid coupling of every support frame has the wheel to stop.
The utility model has the advantages that: through the precise temperature control adjusting device and the stirring device of the device, the temperature balance of each area in the incubator is realized, the material distribution is uniform, the cells obtain the optimized growth environment, and the culture efficiency is obviously improved. In addition, set up pulley and wheel in the bottom of cell culture ware and stop, can also fix at any time when can removing the culture ware, improved the utility model discloses the practicality of device.
Drawings
FIG. 1 is a schematic structural view of an agitation type temperature-controlled cell culture apparatus according to the present invention;
FIG. 2 is a schematic view of a rotary motor of the stirring type temperature-controlled cell culture device of the present invention;
FIG. 3 is a schematic view of an observation window of the stirring temperature-controlled cell culture device of the present invention.
In the figure: 100. the multifunctional water heater comprises a shell, 200 parts of a culture dish, 300 parts of a temperature sensor, 310 parts of a controller, 320 parts of a display screen, 330 parts of a circulating pump, 340 parts of a water pipe, 350 parts of a heating pipe, 400 parts of a rotating motor, 410 parts of a stirring rod, 420 parts of a stirring blade, 500 parts of a disinfection lamp, 510 parts of a lamp shade, 600 parts of an observation window, 700 parts of a support frame, 710 parts of a pulley and 720 parts of a wheel brake.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
As shown in FIG. 1, a schematic structural diagram of an agitation type temperature-controlled cell culture apparatus of the present invention comprises a housing 100, a culture dish 200 is disposed inside the housing 100, an agitating device is fixedly connected to the top of an inner cavity of the culture dish 200, the agitating device comprises a rotating electrical machine 400, the rotating electrical machine 400 is fixedly connected to the top of the inner cavity of the culture dish 200, an agitating rod 410 is embedded in a linkage shaft of the rotating electrical machine 400, a plurality of detachable agitating blades 420 are fixedly connected to a side wall of the agitating rod 410, as shown in FIG. 2, a temperature sensor 300 is further fixedly connected to the top of the inner cavity of the culture dish 200, a controller 310 is fixedly connected to the bottom of the housing 100, a display screen 320 is fixedly connected to one side of the exterior of the housing 100, a circulating pump 330 for cooling the culture dish 200 is further disposed at the top of the exterior of the culture dish 200, the circulating pump 330 is connected to a water pipe 340, specifically, shell 100 is used as protector, and culture dish 200 is used for placing the cell sap, and circulating pump 330 drives the rivers in the water pipe 340 and carries out circulative cooling for lower the temperature to culture dish 200, heating pipe 350 are used for heating to the temperature in culture dish 200, thereby improve the utility model discloses the practicality of device, universal driving shaft on the rotating electrical machines 400 drive stirring leaf 420 on the puddler 410 and rotate, carry out even stirring, have improved cultivation efficiency and accuracy.
The equal rigid coupling in inner chamber both sides of culture dish 200 has sterilamp 500, and every sterilamp 500 all coats outward has lamp shade 510, and lamp shade 510 passes through bolt threaded connection with culture dish 200.
As shown in fig. 3, the outer wall is embedded with an observation window 600, the observation window 600 is a glass observation window, the observation window 600 is connected with the casing 100 by a bolt and a thread, and in particular, the observation window 600 is used for facilitating a user to observe the culture condition and process of cells in the culture dish 200.
A pair of support frames 700 is fixedly installed at the bottom of the housing 100, each support frame 700 is in threaded connection with the bottom of the housing 100 through a bolt, the bottom of each support frame 700 is fixedly connected with a pulley 710, and one side of each support frame 700 is fixedly connected with a wheel brake 720. Specifically, support frame 700 is used for improving the utility model discloses the practicality of device, pulley 710 are mobile device, convenient operation, and the wheel is stopped 720 and is used for fixed pulley 710, has improved the utility model discloses the stability of device.
The temperature sensor 300 is connected with the input end of the controller 310 through a connecting line, the display screen 320, the circulating pump 330, the heating pipe 350 and the output end of the rotating motor 400 and the controller 310 are connected through a connecting line, the controller 310 is connected with a power supply to be powered on, specifically, the temperature sensor 300 transmits collected data to the controller 310, the collected data is transmitted to the display screen 320 from the controller 310 to be displayed, and the circulating pump 330 and the heating pipe 350 are used according to requirements.
The utility model discloses a concrete working process does: in specific use, the user places the cell sap in the culture dish 200, open the rotating electrical machines 400, the last rotation axis of rotating electrical machines 400 drives stirring leaf 420 on the puddler 410 and evenly stirs, temperature sensor 300 reaches controller 310 with the temperature value in the culture dish 200, controller 310 conveys and shows on the display screen 320, the user uses heating pipe 350 to heat according to the demand, when the higher needs of the degree of enlightenment cool down, open circulating pump 330 and carry out the cooling water cooling to culture dish 200, observe the cell sap in the culture dish 200 through observation window 600.
Claims (6)
1. The utility model provides a stirring type control by temperature change cell culture ware, its characterized in that, including shell (100), the inside of shell (100) is equipped with culture dish (200), inner chamber top rigid coupling temperature sensor (300) and agitating unit of culture dish (200), and the outside bottom of culture dish (200) is equipped with heating pipe (350), bottom rigid coupling controller (310) of shell (100), outside one side rigid coupling display screen (320) of shell (100), culture dish (200) outside still are equipped with circulating pump (330) that are used for cooling culture dish (200), and temperature sensor (300) pass through the connecting wire with the input of controller (310) and are connected, and display screen (320), circulating pump (330) and heating pipe (350) pass through the wire with the output of controller (310) and are connected.
2. The stirring type temperature-controlled cell culture device according to claim 1, wherein the circulating pump (330) is disposed on the top of the culture dish (200), the circulating pump (330) is connected to the water pipe (340), and one end of the water pipe (340) surrounds the outer wall of the culture dish (200) from top to bottom and is connected to the circulating pump (330).
3. The stirring type temperature-controlled cell culture device according to claim 1, wherein the stirring device comprises a rotating motor (400), the rotating motor (400) is connected with the output end of the controller (310), the rotating motor (400) is fixedly connected to the top of the inner cavity of the culture dish (200), a stirring rod (410) is embedded in a linkage shaft of the rotating motor (400), and a plurality of detachable stirring blades (420) are fixedly connected to the side wall of the stirring rod (410).
4. The stirring type temperature-controlled cell culture device according to claim 1, wherein disinfection lamps (500) are fixedly connected to two sides of the inner cavity of the culture dish (200), a lampshade (510) is sleeved outside each disinfection lamp (500), and the lampshade (510) is in threaded connection with the culture dish (200) through a bolt.
5. The temperature-controlled stirring cell culture device as claimed in claim 1, wherein a glass observation window (600) is embedded in the outer wall of the housing (100), and the observation window (600) is connected with the housing (100) through a bolt and a thread.
6. The stirring type temperature-controlled cell culture device according to any one of claims 1 to 5, wherein a pair of support frames (700) are fixedly mounted at the bottom of the housing (100), each support frame (700) is in threaded connection with the bottom of the housing (100) through a bolt, a pulley (710) is fixedly connected to the bottom of each support frame (700), and a wheel brake (720) is fixedly connected to one side of each support frame (700).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020578323.XU CN212451443U (en) | 2020-04-17 | 2020-04-17 | Stirring type temperature control cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020578323.XU CN212451443U (en) | 2020-04-17 | 2020-04-17 | Stirring type temperature control cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212451443U true CN212451443U (en) | 2021-02-02 |
Family
ID=74471601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020578323.XU Expired - Fee Related CN212451443U (en) | 2020-04-17 | 2020-04-17 | Stirring type temperature control cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212451443U (en) |
-
2020
- 2020-04-17 CN CN202020578323.XU patent/CN212451443U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210202 |