CN212451438U

CN212451438U - Cell culture device

Info

Publication number: CN212451438U
Application number: CN201922208704.9U
Authority: CN
Inventors: 不公告发明人
Original assignee: Shanghai Fushen Biotechnology Co ltd
Current assignee: Shanghai Fushen Biotechnology Co ltd
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2021-02-02
Anticipated expiration: 2029-12-11

Abstract

The utility model discloses a cell culture device, which comprises a nutrient solution treatment device and a cell growth device, wherein the lower side wall surface of the nutrient solution treatment device is connected with the cell growth device through a liquid discharge pipe, the upper side wall surface of the nutrient solution treatment device is fixedly connected with an installation box, and the inner side wall surface of the installation box is fixedly connected with a stirring mechanism and an auxiliary mechanism; the auxiliary mechanism is added in the original stirring device and used for assisting the stirring work to enable the bottom nutrient solution to be stirred up and down along with the auxiliary plate, and the nutrient solution is stirred more fully in the stirring process.

Description

Cell culture device

Technical Field

The utility model relates to a cell culture technical field specifically is a cell culture device.

Background

Cell culture generally refers to all in vitro cultures, and the existing methods for supplying nutrient solution for cell culture generally comprise: inoculate the cell in the reactor, rethread pipeline supplies its growth for cell or tissue provide nutrient solution, and simultaneously, for the needs of cell growth environment, still be equipped with the pipeline that can let in gas, published utility model patent (CN201720928820.6) "a cell culture device", this utility model well be used for stirring the rabbling mechanism of nutrient solution and only can provide horizontal stirring, when being applied to the nutrient solution of different density, because its fixed stirring law, cause the nutrient solution that stagnates in the bottom can't participate in the stirring process, can lead to the stirring inequality of nutrient solution, produce the data error to subsequent cell culture work, lead to experiment failure etc..

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell culture device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell culture device, includes nutrient solution processing apparatus and cell growth device, nutrient solution processing apparatus's downside wall passes through the fluid-discharge tube and links to each other with cell growth device, nutrient solution processing apparatus's upside wall fixedly connected with install bin, the inside wall fixedly connected with rabbling mechanism and the complementary unit of install bin, the rabbling mechanism includes the mounting bracket, the first rotating electrical machines of inside wall fixedly connected with of mounting bracket, the downside wall meshing of first rotating electrical machines is connected with first gear, the outside wall meshing of first gear is connected with the second gear, the outside wall fixedly connected with axis of rotation of second gear, the axis of rotation rotates the interior wall face of connecting in nutrient solution processing apparatus.

Preferably, the auxiliary mechanism includes the skid, skid fixed connection is in the inside wall face of install bin, the inside wall face sliding connection of skid has the action piece, the first tooth of inside wall face fixed connection of action piece and second tooth, the inside wall face meshing of first tooth is connected with the auxiliary wheel, the outside wall meshing of auxiliary wheel is connected with the second rotating electrical machines, the downside wall fixed connection of action piece has the auxiliary rod, auxiliary rod sliding connection is in the inside wall face of axis of rotation, first rotating electrical machines and second rotating electrical machines are connected with the external power source electricity.

Preferably, the lower end of the auxiliary rod is fixedly connected with an auxiliary shaft, and the outer side wall surface of the auxiliary shaft is rotatably connected with an auxiliary plate.

Preferably, the outer side wall surface of the nutrient solution treatment device is fixedly connected with a liquid inlet pipe and an air inlet pipe, the inner side wall surface of the nutrient solution treatment device is fixedly connected with a sterilization device and a heating device, and the sterilization device and the heating device are both electrically connected with an external power supply.

Preferably, a product pipe and a waste pipe are fixedly connected to the bottom of the cell growth device.

Preferably, the outer side wall surface of the rotating shaft is fixedly connected with a paddle.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model improves the stirring device, so that the blades in the original stirring device are arranged in a staggered way, and the transverse stirring process is more sufficient; the auxiliary mechanism is added in the original stirring device and used for assisting the stirring work to enable the bottom nutrient solution to be stirred up and down along with the auxiliary plate, and the nutrient solution is stirred more fully in the stirring process.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an enlarged view at a.

In the figure: 1. nutrient solution processing apparatus, 11, feed liquor pipe, 12, intake pipe, 13, sterilization apparatus, 14, heating apparatus, 2, cell growth apparatus, 21, product pipe, 22, waste pipe, 3, drain pipe, 4, installation box, 5, stirring mechanism, 51, mounting rack, 52, first rotating electrical machines, 53, first gear, 54, second gear, 55, axis of rotation, 56, paddle, 6, auxiliary mechanism, 61, carriage, 62, action block, 63, first tooth, 64, second tooth, 65, auxiliary wheel, 66, second rotating electrical machines, 67, auxiliary rod, 68, auxiliary shaft, 69, auxiliary plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: a cell culture device comprises a nutrient solution treatment device 1 and a cell growth device 2, wherein the lower side wall surface of the nutrient solution treatment device 1 is connected with the cell growth device 2 through a drain pipe 3, the upper side wall surface of the nutrient solution treatment device 1 is fixedly connected with an installation box 4, the inner side wall surface of the installation box 4 is fixedly connected with a stirring mechanism 5 and an auxiliary mechanism 6, the stirring mechanism 5 comprises an installation frame 51, the inner side wall surface of the installation frame 51 is fixedly connected with a first rotating motor 52, the lower side wall surface of the first rotating motor 52 is connected with a first gear 53 in a meshing way, the outer side wall surface of the first gear 53 is connected with a second gear 54 in a meshing way, the outer side wall surface of the second gear 54 is fixedly connected with a rotating shaft 55, the rotating shaft 55 is rotatably connected with the inner side wall surface of the nutrient solution treatment device 1, when stirring is carried out, an external power supply of the first rotating motor 52 is turned on to start, the second gear 54 engaged with the first gear 53 rotates counterclockwise, and the rotating shaft 55 fixedly connected to the second gear 54 also rotates counterclockwise.

Specifically, the auxiliary mechanism 6 includes a sliding frame 61, the sliding frame 61 is fixedly connected to the inner side wall surface of the mounting box 4, the inner side wall surface of the sliding frame 61 is slidably connected with a moving block 62, the inner side wall surface of the moving block 62 is fixedly connected with a first tooth 63 and a second tooth 64, the inner side wall surface of the first tooth 63 is engaged with an auxiliary wheel 65, the outer side wall surface of the auxiliary wheel 65 is engaged with a second rotating motor 66, the lower side wall surface of the moving block 62 is fixedly connected with an auxiliary rod 67, the auxiliary rod 67 is slidably connected to the inner side wall surface of the rotating shaft 55, the first rotating motor 52 and the second rotating motor 66 are electrically connected with an external power supply, the second rotating motor 66 is started to drive the auxiliary wheel 65 to rotate clockwise, the first tooth 63 engaged with the auxiliary wheel 65 moves upward, the first tooth 63 is fixedly connected to the inner side wall surface of the moving block 62, that is, the, when the teeth of the auxiliary gear 65 are meshed with the second teeth 64, the second teeth 64 move downwards, and similarly, the action block moves downwards, so that when the second rotating motor 66 is started, the auxiliary gear 65 is driven to rotate, the action block 62 is driven to reciprocate up and down, the auxiliary rod 67 is driven to link the auxiliary plate 69 to vertically stir the internal nutrient solution, and the stirring operation is assisted.

Specifically, an auxiliary shaft 68 is fixedly connected to the lower end of the auxiliary rod 67, an auxiliary plate 69 is rotatably connected to the outer wall surface of the auxiliary shaft 68, and the auxiliary plate 69 rotates with the liquid on the outer wall surface of the auxiliary shaft 68 to assist the stirring operation.

Specifically, the outer side wall surface of the nutrient solution treatment device 1 is fixedly connected with a liquid inlet pipe 11 and an air inlet pipe 12, the inner side wall surface of the nutrient solution treatment device 1 is fixedly connected with a sterilization device 13 and a heating device 14, and the sterilization device 13 and the heating device 14 are both electrically connected with an external power supply.

Specifically, a product pipe 21 and a waste pipe 22 are fixedly connected to the bottom of the cell growth device 2, and the well-stirred nutrient solution enters the cell growth device 2 through the drain pipe 3 to perform cell culture operation, so that the residual solution and the product can be discharged through the product pipe 21 and the waste pipe 22.

Specifically, the paddles 56 are fixedly connected to the outer wall surface of the rotating shaft 55, the paddles 56 on the outer wall surface of the rotating shaft 55 stir the nutrient solution in the transverse direction, and the paddles 56 are arranged in a staggered manner to stir the nutrient solution in multiple transverse directions.

The working principle is as follows: when the device is used, nutrient solution is input from the liquid inlet pipe 11, oxygen or carbon dioxide is input into the nutrient solution treatment device 1 through the air inlet pipe 12, and the sterilization device 13 and the heating device 14 are opened to carry out ultraviolet sterilization and heating treatment on the internal nutrient solution;

when stirring is carried out again, an external power supply of the first rotating motor 52 is turned on to start the first rotating motor, the first gear 53 meshed and connected with the first rotating motor rotates clockwise, the second gear 54 meshed and connected with the first gear 53 rotates anticlockwise, and the rotating shaft 55 fixedly connected with the second gear 54 also rotates anticlockwise, so that the blades 56 fixed on the outer side wall surface of the rotating shaft 55 transversely stir the nutrient solution, and the blades 56 are arranged in a staggered manner and can stir at multiple transverse latitudes; the second rotating motor 66 is started to drive the auxiliary wheel 65 to rotate clockwise, the first tooth 63 meshed and connected with the auxiliary wheel 65 moves upwards, the first tooth 63 is fixedly connected to the inner side wall surface of the moving block 62, namely, the moving block 62 moves upwards, when the tooth of the auxiliary wheel 65 is meshed and connected with the second tooth 64, the second tooth 64 moves downwards, and similarly, the moving block moves downwards, therefore, when the second rotating motor 66 is started, the auxiliary wheel 65 is driven to rotate, the moving block 62 moves up and down in a reciprocating mode, the auxiliary rod 67 is driven to link the auxiliary plate 69 to vertically and longitudinally stir the internal nutrient solution, and the stirring operation is assisted;

the well-stirred nutrient solution enters the cell growth device 2 through the drain pipe 3 for cell culture operation, and the residual solution and the product can be output through the product pipe 21 and the waste pipe 22.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A cell culture device comprises a nutrient solution treatment device (1) and a cell growth device (2), wherein the lower side wall surface of the nutrient solution treatment device (1) is connected with the cell growth device (2) through a drain pipe (3), and the cell culture device is characterized in that: the utility model discloses a nutrient solution processing apparatus, including nutrient solution processing apparatus's (1) upside wall fixedly connected with install bin (4), the inside wall fixedly connected with rabbling mechanism (5) and complementary unit (6) of install bin (4), rabbling mechanism (5) include mounting bracket (51), the first rotating electrical machines (52) of inside wall fixedly connected with of mounting bracket (51), the downside wall meshing of first rotating electrical machines (52) is connected with first gear (53), the outside wall meshing of first gear (53) is connected with second gear (54), the outside wall fixedly connected with axis of rotation (55) of second gear (54), axis of rotation (55) are rotated and are connected in the inside wall of nutrient solution processing apparatus (1).

2. A cell culture apparatus according to claim 1, wherein: the auxiliary mechanism (6) comprises a sliding frame (61), the sliding frame (61) is fixedly connected to the inner side wall surface of the installation box (4), the inner side wall surface of the sliding frame (61) is connected with a moving block (62) in a sliding mode, the inner side wall surface of the moving block (62) is fixedly connected with a first tooth (63) and a second tooth (64), the inner side wall surface of the first tooth (63) is connected with an auxiliary wheel (65) in a meshed mode, the outer side wall surface of the auxiliary wheel (65) is connected with a second rotating motor (66) in a meshed mode, the lower side wall surface of the moving block (62) is fixedly connected with an auxiliary rod (67), the auxiliary rod (67) is connected to the inner side wall surface of the rotating shaft (55) in a sliding mode, and the first rotating motor (52) is electrically connected with the second rotating motor (66) through an external power.

3. A cell culture assembly according to claim 2, wherein: the lower end of the auxiliary rod (67) is fixedly connected with an auxiliary shaft (68), and the outer side wall surface of the auxiliary shaft (68) is rotatably connected with an auxiliary plate (69).

4. A cell culture apparatus according to claim 1, wherein: the nutrient solution treatment device is characterized in that a liquid inlet pipe (11) and an air inlet pipe (12) are fixedly connected to the outer side wall surface of the nutrient solution treatment device (1), a sterilization device (13) and a heating device (14) are fixedly connected to the inner side wall surface of the nutrient solution treatment device (1), and the sterilization device (13) and the heating device (14) are both electrically connected with an external power supply.

5. A cell culture apparatus according to claim 1, wherein: the bottom of the cell growth device (2) is fixedly connected with a product pipe (21) and a waste pipe (22).

6. A cell culture apparatus according to claim 1, wherein: and the outer side wall surface of the rotating shaft (55) is fixedly connected with a paddle (56).