CN212434061U - Mouse organ transplantation model blood vessel anastomosis sleeve structure - Google Patents
Mouse organ transplantation model blood vessel anastomosis sleeve structure Download PDFInfo
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- CN212434061U CN212434061U CN202021257595.6U CN202021257595U CN212434061U CN 212434061 U CN212434061 U CN 212434061U CN 202021257595 U CN202021257595 U CN 202021257595U CN 212434061 U CN212434061 U CN 212434061U
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Abstract
The utility model discloses a mouse organ transplantation model blood vessel sleeve structure that coincide, including an outer tube, an inner core, a first wiring and a second wiring. The utility model solves the technical challenges of turning and fixing the blood vessel, reduces the threshold of the operation and the operation complexity through the structural design of the outer tube and the inner core, and ensures that the operation process does not need to use a blood vessel dilator; the person without microsurgery can complete the whole operation after 2 months of training, thus shortening the time for beginner to practice the anastomosis of the small animal organ transplantation fine and micro blood vessels and the whole operation time.
Description
Technical Field
The utility model particularly relates to a mouse organ transplantation model blood vessel sleeve structure that coincide.
Background
The mouse organ transplantation model is an important and effective tool for analyzing ischemia-reperfusion injury, immune rejection and tolerance mechanism in transplantation immunology research. Microsurgical vascular anastomosis in mice is a core link of transplantation operation. The technical difficulty is extremely high, and the wide application of the technology is limited. In 1973, a mouse abdominal ectopic heart transplant model established by Corry through end-to-end suturing became an important milestone for basic transplant immunology research. However, the number of steps involved, the complexity and time consuming of the procedure, and the potential for infection may lead to severe abdominal adhesions and inflammatory reactions, resulting in a less efficient model of such heart transplantation. The technique of ectopic heart transplantation in the neck was first released in 1991. In this model, the external jugular vein of the recipient is anastomosed to the pulmonary artery of the graft and the carotid artery is anastomosed to the aorta. The method has the main advantage that the noninvasive monitoring of the recipient can be conveniently carried out by observing the heart beating. Tomita introduced a modified casing technique in 1997. By this technique, the severed ends of the external jugular vein and carotid artery are passed through the cannula, then the cannula is wrapped 180 degrees out of the edge and inserted over the right pulmonary artery and aorta, respectively, of the donor heart. Finally, the anastomosis is completed by ring ligation and fixation. To date, the cannula technique has been widely applied to a variety of vascularized transplant models, including lung, liver and kidney transplants.
To date, the casing technology has several technical difficulties. For example, most of the current experiments use substitutes such as cuff tubes and intravenous indwelling tubes, and the samples are cut by DIY and lack of specialization. The orifice of the small animal blood vessel does not coincide with the caliber of the small animal blood vessel, which causes blood vessel tearing, elastic change and thrombus. Meanwhile, the carotid artery has a small orifice, which is difficult to fix and open, and is turned 180 ° from the orifice. Microsurgical dilators are often required to accomplish this step. In addition, the tube wall is easy to retract and rebound after being overturned because of the elasticity and the smoothness of the tube wall. Therefore, the technical difficulty is extremely high, and long-term exercise is required. Retrieved, modified vascular cannulas often used anchoring wires or engineered fixation needles to hook the vessel. But only the fixation of the blood vessel is strengthened and the rebound is avoided. The difficulty of adding a design device on a tiny sleeve is high, and the cost is high. But also risks damage and tearing of the blood vessel. And the problems of long preparation time of the vascular cannula, long graft ischemia time, great technical difficulty and the like cannot be solved.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provide a vascular anastomosis sleeve structure of a mouse organ transplantation model.
The utility model discloses a concrete technical scheme as follows:
a vascular anastomosis sleeve structure of a mouse organ transplantation model, which comprises,
the outer wall surface of the outer pipe is provided with a first annular groove and a second annular groove along the axial direction of the outer pipe;
the inner core is provided with a first end, and the first end is of a conical structure;
a first binding thread;
and a second binding thread;
the outer tube and the inner core are in a sleeved connection state and a separated state;
when the sleeve joint state is realized, the first end of the inner core is inserted into the outer tube, and a gap which can clamp the tube wall of the receptor blood vessel is formed between the outer tube and the inner core;
when the donor blood vessel and the receptor blood vessel are in a separated state, the first binding wire and the first annular groove are mutually matched to bind the tube wall of the folded receptor blood vessel to the first annular groove, and the second binding wire and the second annular groove are mutually matched to bind the tube wall of the donor blood vessel to the second annular groove and enable the donor blood vessel and the receptor blood vessel to be communicated.
In a preferred embodiment of the present invention, the length of the inner core is 12 to 15 times the length of the outer tube.
More preferably, the outer tube has an outer diameter of 0.9mm, an inner diameter of 0.7mm and a length of 0.8 mm.
Still more preferably, the inner core has an outer diameter of 0.6mm and a length of 10 mm.
More preferably, the outer tube has an outer diameter of 0.45mm, an inner diameter of 0.30mm and a length of 0.7 mm.
Still more preferably, the inner core has an outer diameter of 0.20mm and a length of 10 mm.
The utility model has the advantages that:
1. the utility model solves the technical challenges of turning and fixing the blood vessel, reduces the threshold of the operation and the operation complexity through the structural design of the outer tube and the inner core, and ensures that the operation process does not need to use a blood vessel dilator; the person without microsurgery can complete the whole operation after 2 months of training, thus shortening the time for beginner to practice the anastomosis of the small animal organ transplantation fine and micro blood vessels and the whole operation time.
2. The utility model reduces the thermal ischemia time of the heart of the donor through the standardized sleeve and the operation design, thereby improving the success rate of transplantation and ensuring more stable operation results. Analysis of ectopic mouse transplantation at > 600 shows that the success rate of heart transplantation can reach 95%. After training, the skilled surgeon can complete the procedure within 35 minutes, with an average of 15.5 minutes for recipient preparation, 10.9 minutes for donor preparation, and 4.4 minutes for donor cardiac anastomosis. The warm and cold ischemia time (from donor preparation to cardiac implantation) is significantly reduced compared to conventional suture and cannula techniques.
Drawings
Fig. 1 is a structural sectional view of an outer tube according to embodiment 1 of the present invention.
Fig. 2 is a structural sectional view of an inner core according to embodiment 1 of the present invention.
Fig. 3 is a structural sectional view of an outer tube according to embodiment 2 of the present invention.
Fig. 4 is a structural sectional view of an inner core according to embodiment 2 of the present invention.
Detailed Description
The technical solution of the present invention will be further illustrated and described below with reference to the accompanying drawings by means of specific embodiments.
Example 1
A mouse organ transplantation model blood vessel anastomosis sleeve structure (the embodiment is used for veins) comprises an external vein tube 1, an internal vein core 2, a first binding thread and a second binding thread (all shown in the figure).
As shown in fig. 1, the outer wall surface of the intravenous tube 1 is provided with a first intravenous annular groove 11 and a second intravenous annular groove 12 along the axial direction thereof;
as shown in fig. 2, the intravenous core 2 has a first end with a tapered configuration;
the intravenous tube 1 and the intravenous core 2 have a sleeved connection state and a separation state;
when in a sleeved state, the first end of the vein inner core 2 is inserted into the vein outer tube 1, and a gap which can clamp the wall of the receptor blood vessel is formed between the vein outer tube 1 and the vein inner core 2;
when in a separated state, the first binding thread and the first vein annular groove 11 are mutually adapted to bind the tube wall of the folded recipient blood vessel to the first vein annular groove 11, and the second binding thread and the second vein annular groove 12 are mutually adapted to bind the tube wall of the donor blood vessel to the second vein annular groove 12 and communicate the donor blood vessel and the recipient blood vessel.
Specifically, the intravenous tube 1 has an outer diameter of 0.9mm, an inner diameter of 0.7mm and a length of 0.8 mm; the vein inner core 2 has an outer diameter of 0.6mm and a length of 10 mm.
Example 2
A vascular anastomosis sleeve structure (for an artery in the embodiment) of a mouse organ transplantation model comprises an external artery tube 1 ', an internal artery core 2', a first binding wire and a second binding wire (all shown in the figure).
As shown in fig. 1, the outer wall surface of the external arterial tube 1 ' is provided with a first arterial annular groove 11 ' and a second arterial annular groove 12 ' along the axial direction thereof;
as shown in fig. 2, the arterial core 2' has a first end having a tapered configuration;
the external artery tube 1 'and the internal artery core 2' have a sleeved connection state and a separation state;
when in a sleeved state, the first end of the artery inner core 2 'is inserted into the artery outer tube 1', and a gap which can clamp the tube wall of the receptor blood vessel is formed between the artery outer tube 1 'and the artery inner core 2';
when in a separated state, the first binding thread and the first artery annular groove 11 'are mutually adapted to bind the tube wall of the folded recipient blood vessel to the first artery annular groove 11', and the second binding thread and the second artery annular groove 12 'are mutually adapted to bind the tube wall of the donor blood vessel to the second artery annular groove 12' and communicate the donor blood vessel and the recipient blood vessel.
Specifically, the outer diameter of the arterial outer tube 1' is 0.45mm, the inner diameter is 0.3mm, and the length is 0.7 mm; the outer diameter of the artery inner core 2' is 0.2mm, and the length is 10 mm.
Example 3
The present embodiment specifically describes the use method of the present invention in example 1 and example 2 by taking the neck of a mouse as an example of heart transplantation, specifically as follows:
1. receptor preparation:
(1) the recipient mice were anesthetized and skin prepared, disinfected, and fixed in the right lateral cervical region.
(2) A lateral incision was made from the lower third of the cervical midline to the right acromioclavicular joint using an ophthalmic scissors.
(3) The right external jugular vein was isolated and ligated with 6-0 silk thread at the distal end.
(4) The proximal end root of the external jugular vein is clamped by a disposable vascular clamp, and then the ligation section is cut off by a pair of microscissors.
(5) The heparinized normal saline cleans the vessel lumen to remove residual blood.
(6) The external jugular vein was passed through the venous cannula with microsurgical straight forceps.
(7) The intravenous core 2 is inserted into the lumen as a stent.
(8) The vessel wall was everted over the venous cannula with microsurgical straight forceps.
(9) The everted vessel is fixed to the outer wall of the venous cannula with an 8-0 wire and snapped into the first venous annular groove 11.
(10) The intravenous core 2 is removed with microsurgical straight forceps.
(11) Blunt dissection was performed adjacent to the carotid artery. The proximal end is clamped by a vascular clamp, the distal end is ligated, and the ligation site is disconnected.
(12) The carotid artery is passed through the arterial cannula and the inner arterial core 2' is inserted into the arterial vessel.
(13) Turning the blood vessel to the artery sleeve by using microsurgery straight forceps;
(14) the everted vessel is fixed to the outer wall of the arterial cannula with a circumferential 8-0 thread and snapped into the first arterial circumferential groove 11'.
(15) The intra-arterial core 2' is removed from the arterial vessel with microsurgical straight forceps.
2. Donor preparation
(1) Anaesthetizing the recipient mice, preparing the skin at the abdomen, disinfecting, and fixing.
(2) Via the inferior vena cava, heparinized donors.
(3) The chest was exposed, the thymus excised, and the pulmonary arteries and veins were severed to reduce cardiac load.
(4) Heparin saline was perfused through the aorta.
(5) The aorta and pulmonary artery were disconnected.
(6) The superior vena cava, inferior vena cava, and pulmonary vein were ligated.
(7) The heart grafts were removed and stored in heparinized saline at 0-4 ℃.
3. Heart implantation
(1) The donor heart was inverted to the right neck incision area of the recipient.
(2) The pulmonary artery of the donor heart was threaded into a 6-0 wire loop with microsurgical straight forceps.
(3) The vein sleeve is wrapped by the vessel lumen, the 6-0 silk thread ring is tightened, fixed on the vein sleeve and clamped into the second vein annular groove 12, and the anastomosis of the pulmonary artery graft and the jugular vein graft is completed.
(4) The same procedure completes the anastomosis of the graft aorta and carotid artery cannula. The 6-0 wire loop was tightened, secured to the arterial cannula and snapped into the second arterial annular groove 12'
(5) The clamped jugular vein and carotid artery were released. A return of sinus rhythm to more than 200 times within 1 minute is considered normal.
Donor hearts were wetted with 37 ℃ saline and examined for bleeding from the grafts. The beating heart was placed in the subcutaneous space and the incision was closed.
The above description is only a preferred embodiment of the present invention, and therefore the scope of the present invention should not be limited by this description, and all equivalent changes and modifications made within the scope and the specification of the present invention should be covered by the present invention.
Claims (6)
1. A mouse organ transplantation model blood vessel anastomosis sleeve structure is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
the outer wall surface of the outer pipe is provided with a first annular groove and a second annular groove along the axial direction of the outer pipe;
the inner core is provided with a first end, and the first end is of a conical structure;
a first binding thread;
and a second binding thread;
the outer tube and the inner core are in a sleeved connection state and a separated state;
when the sleeve joint state is realized, the first end of the inner core is inserted into the outer tube, and a gap which can clamp the tube wall of the receptor blood vessel is formed between the outer tube and the inner core;
when the donor blood vessel and the receptor blood vessel are in a separated state, the first binding wire and the first annular groove are mutually matched to bind the tube wall of the folded receptor blood vessel to the first annular groove, and the second binding wire and the second annular groove are mutually matched to bind the tube wall of the donor blood vessel to the second annular groove and enable the donor blood vessel and the receptor blood vessel to be communicated.
2. The vascular anastomosis sleeve structure according to claim 1, wherein: the length of the inner core is 12-15 times of the length of the outer tube.
3. The vascular anastomosis sleeve structure according to claim 2, wherein: the outer diameter of the outer pipe is 0.9mm, the inner diameter is 0.7mm, and the length is 0.8 mm.
4. The vascular anastomosis sleeve structure according to claim 3, wherein: the outer diameter of the inner core is 0.6mm, and the length of the inner core is 10 mm.
5. The vascular anastomosis sleeve structure according to claim 2, wherein: the outer diameter of the outer pipe is 0.45mm, the inner diameter is 0.30mm, and the length is 0.7 mm.
6. The vascular anastomosis sleeve structure according to claim 5, wherein: the outer diameter of the inner core is 0.2mm, and the length of the inner core is 10 mm.
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