CN110123478A - A kind of vessel cannula and application method with anchoring knot - Google Patents
A kind of vessel cannula and application method with anchoring knot Download PDFInfo
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- CN110123478A CN110123478A CN201910504633.9A CN201910504633A CN110123478A CN 110123478 A CN110123478 A CN 110123478A CN 201910504633 A CN201910504633 A CN 201910504633A CN 110123478 A CN110123478 A CN 110123478A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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Abstract
The invention belongs to vessel cannula technical fields, disclose a kind of vessel cannula and application method with anchoring knot, the application method of the vessel cannula with anchoring knot are as follows: knot is accomplished fluently in lower section in magnetic tape trailer casing in advance, as anchor region；Blood vessel is passed through into casing；Blood vessel is overturn, is pulled to more than anchorage zone, anchoring knot can fix blood vessel and not bounce back；Blood vessel is fixed again in the anchor lines side of tying, and overturning blood vessel is disposably completed.Shell material needed for the present invention is easily obtained, and anchorage zone knot is easy blood vessel overturning extremely, is effectively reduced receptor time, and entire operation can be completed under one man operation, is not necessarily to microsurgery anastomosis, success rate of operation is high, is stable and easy to replicate.Present invention can ensure that overturning disposable success, stable fixation blood vessel, more importantly avoiding overturning inner film injury caused by blood vessel repeatedly in blood vessel；The difficulty of one man operation can be reduced simultaneously.
Description
Technical field
The invention belongs to vessel cannula technical field more particularly to a kind of vessel cannulas and user with anchoring knot
Method.
Background technique
Currently, the prior art commonly used in the trade is such that
The immune related work of heart transplant deepens continuously, and requires increasingly to improve between germline degree of purification confession, receptor, and
Mouse has many advantages, such as strain compared with pure, molecular biology reagents source is more, immunology detection is convenient, at low cost, thus in the modern times
The status for having its irreplaceable in transplantation immunology research.Since mouse body is small, there are certain technology hardly possiblies for identical thin vessels
Degree, limits the extensive use of mouse heart transplantation model.It is existing successfully by Cuff Technology application to mouse heart transplantation model
On, greatly reduce operating difficulty, but domestic report is less, may be limited to domestic microsurgery laboratory technical conditions and
Specialized barrel is difficult to obtain.Using discovery when domestic and international report tail-Cuff technology overturning blood vessel by individual mice blood vessel elasticity
Differentia influence, the technology are very unstable.Nature retraction can occur in the case where no fixation for the blood vessel especially overturn, cause
One man operation is difficult.
In conclusion problem of the existing technology is:
(1) country is limited to the technical conditions in domestic microsurgery laboratory and specialized barrel is difficult to obtain；
Double Stereo microscope price costly, more than general 10 ten thousand, and bulky, need more large-scale experiment room to match
It is standby.Single Stereo microscope is cheap, and about 2000 yuan, required space is smaller.It turns up set currently without the blood vessel specially designed
Pipe often voluntarily cuts the venous indwelling needle cannula of different cultivars as experiment consumptive material according to each laboratory.
(2) using discovery when report tail-Cuff technology overturning blood vessel both at home and abroad by individual mice blood vessel elasticity difference shadow
It rings, the technology is very unstable, and nature retraction can occur in the case where no fixation for the blood vessel especially overturn, leads to single behaviour
Make difficult.
Solve the difficulty of above-mentioned technical problem: by the technology, even if the researcher of microsurgical Basis can be compared with
It is fast to complete one man operation's blood vessel turnover technology.Principle is easy to learn.
Solve the meaning of above-mentioned technical problem: there is also many tera incognitas for clinic organ transplant at present.It is badly in need of basis to grind
Study carefully support.The gene of mouse and the gene goodness of fit of the mankind are close to 99%, are highly desirable research animals.And price
Cheaply, it is easily obtained.But it is higher to carry out heart transplant difficulty for small volume.Especially vascular anastomosis technology strongly limits this
Application of the model in each profession.Current CUFF technology both at home and abroad is subjected to improvement again, so that one man operation is more easier,
Once success rate is high.And greatly reduce Laboratory Request.Mouse heart transplantation model can preferably be promoted each
The application of profession.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of vessel cannula with anchoring knot and users
Method.
The invention is realized in this way a kind of vessel cannula with anchoring knot, the blood vessel with anchoring knot
Casing includes: BD remaining needle casing, stalklet, knot；
26G the and 22G venous indwelling needle cannula of BD remaining needle casing is respectively as artery and vein band tail casing；BD is stayed
Setting needle cannula one end is stalklet, and the middle lower section of BD remaining needle casing is knot.
Further, length is the BD remaining needle casing of 1-2mm.
Further, knot is surgery side's knot, 9-0 silk thread, the long 1-2mm of the end of a thread.
It is described another object of the present invention is to provide the production method of the vessel cannula with anchoring knot described in one kind
The production method of vessel cannula with anchoring knot includes:
The first step is covered BD remaining needle casing 26G and 22G venous indwelling needle cannula as artery and vein band tail
Pipe；It will cut at 2/5ths of casing, do a stalklet in casing one end；
Second step, knot is accomplished fluently in lower section in casing, and as anchor region, anchor lines are become surgery side and tied, 9-0 silk thread,
The long 1-2mm of the end of a thread.
It is described another object of the present invention is to provide the application method of the vessel cannula with anchoring knot described in one kind
The application method of vessel cannula with anchoring knot includes:
The first step, zona vascuiosa tail casing of the production with anchoring knot (by taking C57 mouse as an example)；By BD remaining needle casing
26G and 22G venous indwelling needle cannula is respectively as artery and vein band tail casing；Cannula scissors are broken into as the set of long 3-4mm
Pipe, can do a stalklet in casing one end；Knot is accomplished fluently in lower section in casing in advance, and as anchor region, anchor lines become surgery
Fang Jie, 9-0 silk thread, the long 1-2mm of the end of a thread；
Second step, dissociate mouse vena jugularis externa and arteria carotis communis, and mouse is lain on the back and is fixed on heating operation pad, art area is standby
Skin disinfection；The longitudinal incision skin on the outside of right throat separates subcutaneus adipose tissue, dissection down toward clavicle up to lower jaw basal part
Free vena jugularis externa out；After proximal part is closed with micro vessel clamp folder, nearly cranium side ligation, detachment vein distal end；With heparin compound chlorine
Change blood and blood clot that sodium solution washes away the free section of blood vessel proximal part；Detachment part Anterior jugular muscles, seek on the inside of vena jugularis externa
Look for arteria carotis communis；
Vein is inserted in 22G in its casing by third step, and not damaged vessel forceps clamp casing pipe handle and blood vessel is fixed, with aobvious
The help of micro- tweezer makes wall of vein turn up to be placed on casing, its vascular wall is pulled to well beyond after anchor lines tie region, with 9-0 silk thread
The fixed blood vessel that turns up of edge ligation is tied in anchor lines；It is inserted in arterial cannula 24G, the free section of blood vessel is rinsed with heparin liquid again；By
Body cervical incision is applied with wet saline gauze, waits to be implanted for the heart.
In conclusion advantages of the present invention and good effect are as follows:
The invention enables shell materials to be easily obtained, and blood vessel turns over set and is extremely easy, and effectively reduces for the core ischemia time, and whole
A operation is completed under one man operation, is not necessarily to microsurgery anastomosis, and success rate of operation is high, is stable and easy to replicate.The technology
It can guarantee to overturn disposable success in blood vessel, stable fixation blood vessel avoids overturning inner film injury caused by blood vessel repeatedly.Together
When greatly reduce the difficulty of one man operation.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the vessel cannula provided in an embodiment of the present invention with anchoring knot.
Fig. 2 is the application method flow chart of the vessel cannula provided in an embodiment of the present invention with anchoring knot.
Fig. 3 is the structural schematic diagram of BD remaining needle casing provided in an embodiment of the present invention.
Fig. 4 is the structural schematic diagram that knot provided in an embodiment of the present invention is put into BD remaining needle casing.
Fig. 5 is that blood vessel provided in an embodiment of the present invention and vessel cannula are connected and fixed structural schematic diagram.
In figure: 1, BD remaining needle casing；2, stalklet；3, knot；4, blood vessel clip.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
It elaborates with reference to the accompanying drawing to technical solution of the present invention；
As shown in Fig. 1, Fig. 3, Fig. 4 and Fig. 5, the vessel cannula provided in an embodiment of the present invention with anchoring knot includes:
BD remaining needle casing 1, stalklet 2, knot 3, blood vessel clip 4.
26G the and 22G venous indwelling needle cannula of BD remaining needle casing 1 is respectively as artery and vein band tail casing；BD
1 one end of remaining needle casing is stalklet 2, and the middle lower section of BD remaining needle casing 1 is knot 3.
By BD remaining needle casing 1 (22G, 26G；BD, the U.S.).26G and 22G venous indwelling needle cannula respectively as artery and
Vein band tail casing.BD remaining needle casing 1 is cut (free with specific reference to mouse Cervical Vessels as the casing of long 1-2mm
Length), in order to clamp during surgery conveniently, a stalklet can be done in casing one end, also as tail tail.In advance in casing under
Knot 3 is accomplished fluently by side, and as anchor region, anchor lines become surgery side's knot, and 9-0 silk thread, the end of a thread is about 1-2mm.
Vein is inserted in BD remaining needle casing 1, and not damaged blood vessel clip 4 clamps casing pipe handle and blood vessel is fixed.
As shown in Fig. 2, the production method of the vessel cannula provided in an embodiment of the present invention with anchoring knot includes:
S201: it is covered BD remaining needle casing 26G and 22G venous indwelling needle cannula as artery and vein band tail
Pipe；Half will be cut off at 2/5ths of casing, does a stalklet in casing one end；
S202: mentioning the lower section in casing and accomplish fluently knot, and as anchor region, anchor lines are become surgery side and tied, 9-0 silk thread,
The end of a thread is about 1-2mm.
The application method of vessel cannula provided in an embodiment of the present invention with anchoring knot includes:
The first step, zona vascuiosa tail casing of the production with anchoring knot.By BD remaining needle casing (22G, 26G；BD, beauty
State).26G and 22G venous indwelling needle cannula is respectively as artery and vein band tail casing.Cannula scissors are broken into as long 3-4mm
Casing (with specific reference to mouse Cervical Vessels dissociate length), in order to clamp during surgery conveniently, it is small one can be done in casing one end
Handle, also as tail tail.Knot is accomplished fluently in lower section in casing in advance, and as anchor region, anchor lines become surgery side's knot, 9-
0 silk thread, the end of a thread are about 1-2mm；
Second step, dissociate mouse vena jugularis externa and arteria carotis communis.Mouse is lain on the back and is fixed on heating operation pad, art area is standby
Skin disinfection.The longitudinal incision skin on the outside of right throat separates subcutaneus adipose tissue, dissection down toward clavicle up to lower jaw basal part
Free vena jugularis externa out.After proximal part is closed with micro vessel clamp folder, nearly cranium side ligation, detachment vein distal end.With heparin compound chlorine
Change blood and blood clot that sodium solution (50U/mL) washes away the free section of blood vessel proximal part.The detachment part throat on the inside of vena jugularis externa
Muscle group finds arteria carotis communis, same treatment, careful separation arteria carotis communis and other intrathecal tissues of arteria carotis communis, avoids damage to blood
Pipe, nerve；
Third step, vessel cannula technology.Vein is inserted in its casing (22G), not damaged blood vessel clip clamps casing pipe handle
And blood vessel is fixed, and so that wall of vein is turned up with microforceps help and is placed on casing, its vascular wall is pulled to well beyond anchoring knot
Behind region (the fixed unnatural retraction of blood vessel that turns up), the fixed blood vessel that turns up of edge ligation is tied in anchor lines with 9-0 silk thread.With method set
Enter arterial cannula (24G), the free section of blood vessel is rinsed with heparin liquid again, prevents blood clot from being formed.Receptor cervical incision is applied with wet
Saline gauze waits to be implanted for the heart.
Technical solution of the present invention is described further below with reference to specific implementation；
For embodiment 1:C57 mouse jugular vein
1. donor operation is using intramuscular injection anesthesia (0.5% phenobarbital 3ml), mouse dorsal position enters through median incision
After abdomen, 0~4 DEG C of heparin saline 2ml is injected from inferior caval vein, while cutting abdominal aorta.Diaphram, then edge are cut off after perfusion
Breastbone is cut off to clavicle level in bilateral anterior axillary line, is cooled down after digging thorax with 0~4 DEG C of sodium chloride solution.At binding at hilus pulumonis
It pricks, cuts bilateral lungs, cut aorta ascendens and pulmonary artery in truncus brachiocephalicus proximal end and pulmonary artery crotch, then bunchy ligatures
Superior and inferior vena cave is removed for the heart, is stored in 0~4 DEG C of sodium chloride solution.
The cleaning grade operation 2. receptor is performed the operation.Horizontal position anaesthetizes the isoflurane sucking for 3-5%.Right neck part preserved skin,
Iodophor disinfection.Intraperitoneal injection physiological saline 1ml.Stringer notch is done from right angle of mandible to left clavicle midpoint, extracts right submaxillary gland,
Appear from proximal part and the right vena jugularis externa that dissociates is to crotch, removes circumvascular fat and other soft tissues.Dissociate right neck
Inside and outside total artery to neck at aortic bifurcation.Above-mentioned two blood vessels are blocked in proximal part, in crotch ligation, cutting.Respectively from
It is pierced by the casing of well in advance, then blood vessel turns up again and is placed on casing, so that endangium is turned up in sleeve surface, use
9-0 knot, which is pricked, fixes.
3. heart transplant will be placed in receptor neck for the heart, the apex of the heart is towards receptor head side.First vein end casing is included in for the heart
Then arterial end casing is included in for cardiac aorta, with 9-0 silk thread cerclage with method cerclage by pulmonary artery.Arteriovenous casing cerclage is complete
Finish, first open vein end blood vessel clip, then adjust the position of heart transplantation, smooth artery and vein traveling slowly opens arterial end, moves
Heart color is planted to be switched to scarlet heart by pale and switched to regular bounce after of short duration ventricular fibrillation.It sews up the incision skin.
What is be most difficult in the operation is in mouse receptor preparation process, and vena jugularis externa and arteria carotis communis turn up and be fixed on blood
In pipe-in-pipe.
Embodiment 2:
The present invention also uses the technology to establish technique of cervical heterotopic heart transplantation in mice, and is simplified and improved, and adopts simultaneously
With 26G and 22G venous indwelling needle cannula respectively as artery and vein band tail casing, we carry out " anchor in advance on casing
It is fixed " knot (9-0 silk thread, the end of a thread are about 1-2mm).So that shell material is easily obtained, blood vessel turns over set and is extremely easy, and effectively reduces
For the core ischemia time, and entire operation is completed under one man operation, is not necessarily to microsurgery anastomosis, success rate of operation is high, surely
It is easy to replicate calmly.The technology can guarantee to overturn disposable successful, stable fixation blood vessel in blood vessel, avoid overturning blood vessel repeatedly
Caused inner film injury.The difficulty of one man operation is greatly reduced simultaneously.
The device of the invention can change other experimental animals, can be according to blood vessel diameter appropriate adjustment remaining needle thickness.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (5)
1. a kind of vessel cannula with anchoring knot, which is characterized in that the vessel cannula with anchoring knot includes: BD
Remaining needle casing, knot；
26G the and 22G venous indwelling needle cannula of BD remaining needle casing is respectively as artery and vein band tail casing；BD remaining needle
The middle lower section of casing is knot.
2. the vessel cannula as described in claim 1 with anchoring knot, which is characterized in that BD Injector Bushing length of tube uses
The BD remaining needle casing of 3mm-4mm.
3. the vessel cannula as described in claim 1 with anchoring knot, which is characterized in that knot is that surgery side is tied, 9-0
Line, the long 1-2mm of the end of a thread.
4. a kind of production method of the vessel cannula as described in claim 1 with anchoring knot, which is characterized in that described to have
The production method of vessel cannula for being anchored knot includes:
The first step, using BD remaining needle casing 26G and 22G venous indwelling needle cannula as artery and vein with tail casing；
Half will be cut off at 2/5ths of casing, does a stalklet, i.e. tail tail in casing one end；
Second step, knot is accomplished fluently in lower section in casing, and as anchor region, anchor lines become surgery side's knot, 9-0 silk thread, the end of a thread
Long 1-2mm.
5. a kind of application method of the vessel cannula as described in claim 1 with anchoring knot, which is characterized in that described to have
The application method of vessel cannula for being anchored knot includes:
The first step, zona vascuiosa tail casing of the production with anchoring knot；By BD remaining needle casing 26G and 22G venous detaining needle
Casing is respectively as artery and vein band tail casing；Cannula scissors are broken into as the casing of long 3-4mm, one can be done in casing one end
Stalklet；Knot is accomplished fluently in lower section in casing in advance, and as anchor region, anchor lines are become surgery side and tied, 9-0 silk thread, and the end of a thread is long
1-2mm；
Second step, dissociate mouse vena jugularis externa and arteria carotis communis, and mouse is lain on the back and is fixed on heating operation pad, art area preserved skin disappears
Poison；The longitudinal incision skin on the outside of right throat separates subcutaneus adipose tissue down toward clavicle up to lower jaw basal part, and dissection is free
Vena jugularis externa out；After proximal part is closed with micro vessel clamp folder, nearly cranium side ligation, detachment vein distal end；With heparin compound NaCl
Solution washes away the blood and blood clot of the free section of blood vessel proximal part；The detachment part Anterior jugular muscles on the inside of vena jugularis externa, find neck
Total artery；
Vein is inserted in 22G in its casing by third step, and not damaged vessel forceps clamp casing pipe handle and blood vessel is fixed, with microforceps
Help makes wall of vein turn up to be placed on casing, its vascular wall is pulled to well beyond after anchor lines tie region, with 9-0 silk thread in anchor
Alignment ties the fixed blood vessel that turns up of edge ligation；It is inserted in arterial cannula 24G, the free section of blood vessel is rinsed with heparin liquid again；Receptor neck
Portion's notch is applied with wet saline gauze, waits to be implanted for the heart.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910504633.9A CN110123478A (en) | 2019-06-12 | 2019-06-12 | A kind of vessel cannula and application method with anchoring knot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910504633.9A CN110123478A (en) | 2019-06-12 | 2019-06-12 | A kind of vessel cannula and application method with anchoring knot |
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| CN110123478A true CN110123478A (en) | 2019-08-16 |
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| CN201910504633.9A Pending CN110123478A (en) | 2019-06-12 | 2019-06-12 | A kind of vessel cannula and application method with anchoring knot |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112294381A (en) * | 2020-10-27 | 2021-02-02 | 中南大学湘雅医院 | Sleeve device for microsurgery blood vessel and lymphatic vessel anastomosis |
| CN112998894A (en) * | 2021-03-08 | 2021-06-22 | 首都医科大学附属北京朝阳医院 | Anastomotic cannula for establishing rat inferior vena cava replacement model |
| CN114391978A (en) * | 2022-01-21 | 2022-04-26 | 宁波市第六医院 | Needle insertion type severed finger replantation vein micro-support |
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Application publication date: 20190816 |