CN212357267U

CN212357267U - Filtration auxiliary structure of cell culture container

Info

Publication number: CN212357267U
Application number: CN202020802149.2U
Authority: CN
Inventors: 周宏�; 孔辉; 解卫平; 黄文�; 刘萍
Original assignee: Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Current assignee: Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2021-01-15
Anticipated expiration: 2030-05-14

Abstract

The utility model discloses a filtration auxiliary structure of cell culture container, including rose box and the culture dish of setting in rose box inside, the left and right sides at rose box top all is connected with the inlet pipe, the horizontal division board of fixedly connected with between the rose box inner chamber left and right sides, the vertical division board of fixedly connected with between the middle part of horizontal division board top and bottom and the top of rose box inner chamber and the bottom, the utility model relates to a cell culture technical field in the medical science scientific research. This cell culture container's filtration auxiliary structure utilizes the division board to divide into four studio with the rose box, for two filter work of rose box develop simultaneously and provide the condition, is convenient for set up the comparative example, makes the test result have convincing power more, and at the inside transfer mechanism that sets up of rose box for deposit the nutrient medium to the interpolation and preheat, carries out the temperature regulation and control through built-in accuse temperature mechanism to two filter chambers in the rose box, the more utilization accuse temperature mechanism that increases.

Description

Filtration auxiliary structure of cell culture container

Technical Field

The utility model relates to a cell culture technical field in the medical science scientific research specifically is a filtration auxiliary structure of cell culture container.

Background

Cell culture is an important component in medical research. Researchers often need to add different chemicals to the cell culture medium in order to truly mimic the pathophysiological environment in which disease occurs, in order to induce cells within the culture vessel to produce the corresponding disease phenotype. The cell culture medium needs to be sterile during the culture process. Compared with filtration sterilization, the work intensity of autoclaving is smaller, the cost is lower, but the loss of nutrient components is easily caused, secondary pollution is easily caused in the process of multiple sample adding (such as medicines, cytokines or nutrient substances and the like), the filtration sterilization can reduce the nutrient loss of the culture medium to the minimum extent, and simultaneously can avoid the generation of a large amount of bubbles, so that the development direction of culture medium sterilization is provided at present. Traditional filter equipment generally can only carry out the single when filtering, consequently often need filter many times in succession just can get rid of miscellaneous fungus completely and dissolve the material, this not only waste time, increase work load, the filter effect also is difficult to guarantee moreover. In addition, the growth of cells must be ensured to be constant and appropriate temperature, the standard temperature of most of the current cell culture is 36.5 ℃ +/-0.5 ℃, but the traditional filtering device cannot detect and adjust the temperature of a cell culture medium in the filtering process, so that the follow-up research on cell morphology, functionality and behavior is influenced.

For example, patent No. CN207276631U discloses a filtration auxiliary structure of cell culture container, it is through being provided with the attemperator, is provided with temperature sensor and intelligent temperature controller in the inside of attemperator, and temperature sensor and intelligent temperature controller are connected electrically each other, and temperature sensor can the inside temperature of real-time detection attemperator, and when the temperature was too high or crossed low, the temperature in the attemperator can be adjusted to intelligent temperature controller, maintains the suitable temperature of vigorous growth of cell. Be connected with the handle in the upper end of upper cover plate, the tempering tank that can conveniently take, be provided with filtration membrane in bottom open-ended inside, be provided with the filter screen in the inside of honeycomb duct, the filter screen can further filter, it needs to go on repeatedly and waste time many times to have solved the filtration degerming, increase the problem of work load, in the direct incubator that gets into of material after the filtration, need not to shift repeatedly, also can satisfy the required nutritional requirement of cell culture when reducing the secondary pollution probability, but this kind of tempering tank utilization efficiency is lower, the inside filter screen that sets up of pipeline very influences follow-up cleaning simultaneously, and when adding nutritive material, because the temperature is inhomogeneous, influence cell growth easily.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a cell culture container's filtration auxiliary structure has solved the temperature regulation case utilization efficiency lower, and the filter screen of the inside setting of pipeline very influences follow-up cleaning simultaneously to when adding nutritive material, because the temperature is inhomogeneous, influence the problem of cell growth easily.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: a filtration auxiliary structure of a cell culture container comprises a filter box and a culture dish arranged inside the filter box, wherein the left side and the right side of the top of the filter box are both connected with inlet pipes, a transverse partition plate is fixedly connected between the left side and the right side of an inner cavity of the filter box, a longitudinal partition plate is fixedly connected between the middle part of the top and the bottom of the transverse partition plate and the top and the bottom of the inner cavity of the filter box, filter basins are arranged at the top of the transverse partition plate and at the left side and the right side of the longitudinal partition plate, a transfer mechanism is fixedly connected at the left side and the right side of the inner cavity of the filter box and comprises a storage hopper, a connecting plate and a connecting rope, the bottom of the storage hopper is rotatably connected with the inner surface of the connecting plate through a rotating rod, one side of the storage hopper, which is close to the inner wall of the filter box, is fixedly connected with one, one side of the connecting plate is fixedly connected with the inner wall of the filter box.

Preferably, the surface of the longitudinal isolation plate is fixedly connected with a temperature control mechanism above the transverse isolation plate.

Preferably, the equal fixedly connected with fixed block in bottom of rose box left and right sides, the left and right sides of rose box inner chamber just is located the connecting plate and connects equal fixedly connected with gag lever post between the rope.

Preferably, the internal surface of filtering basin is provided with the filter plate, the bottom intercommunication of filtering basin has the discharging pipe, and the culture dish sets up the below at the discharging pipe.

Preferably, the left and right sides at positive top of rose box all articulates through the hinge has first sealing door, the positive bottom of rose box articulates through the hinge has the second sealing door.

Preferably, the surface of the first sealing door is provided with a transparent observation window.

Advantageous effects

The utility model provides a filtration auxiliary structure of cell culture container. Compared with the prior art, the method has the following beneficial effects:

(1) the filtration auxiliary structure of the cell culture container comprises a filter box, wherein the left side and the right side of the top of the filter box are both connected with a feeding pipe, a transverse partition plate is fixedly connected between the left side and the right side of an inner cavity of the filter box, the middle parts of the top and the bottom of the transverse partition plate and a longitudinal partition plate are fixedly connected between the top and the bottom of the inner cavity of the filter box, the left side and the right side of the top of the transverse partition plate and the left side of the longitudinal partition plate are both provided with a filtration basin, the left side and the right side of the inner cavity of the filter box are both fixedly connected with a transfer mechanism, the transfer mechanism comprises a storage hopper, a connecting plate and a connecting rope, the bottom of the storage hopper is rotationally connected with the inner surface of the connecting plate through a rotating rod, one side of the storage hopper, which is close to the inner wall, the cooperation rose box, the culture dish, the inlet pipe, horizontal division board, vertical division board, filter the basin, transfer the mechanism, deposit the fill, the setting of connecting plate and connection rope, utilize horizontal division board and vertical division board to divide into four studios with the rose box, develop simultaneously for two filter work of rose box and provide the condition, be convenient for set up the comparative example, make the test result have persuasion more, and at the inside transfer mechanism that sets up of rose box, be used for depositing the preheating to the nutrient medium of interpolation, two filter chambers to the rose box through built-in accuse temperature mechanism carry out the temperature regulation and control, the temperature control mechanism that utilizes of bigger change.

(2) The filter auxiliary structure of the cell culture container comprises fixing blocks fixedly connected to the bottoms of the left side and the right side of a filter box, limiting rods fixedly connected to the left side and the right side of an inner cavity of the filter box and positioned between a connecting plate and a connecting rope, first sealing doors hinged to the left side and the right side of the front top of the filter box through hinges, second sealing doors hinged to the front bottom of the filter box through hinges, transparent observation windows arranged on the surfaces of the first sealing doors, and the connecting rope fixed by the fixing blocks in cooperation with the fixing blocks, the limiting rods, the first sealing doors, the second sealing doors and the transparent observation windows, the position of the storage hopper is limited by the limit rod, so that the materials in the feeding pipe can be completely dropped into the storage hopper, and meanwhile, the transparent observation window is arranged on the sealing door, so that the observation of experimenters is facilitated, and the experiment can be carried out more conveniently.

Drawings

Fig. 1 is a schematic external structural view of the present invention;

FIG. 2 is a cross-sectional view of the structure of the present invention;

fig. 3 is a cross-sectional view of the filter plate structure of the present invention.

In the figure: 1. a filter box; 2. a culture dish; 3. a feed pipe; 4. a transverse separator plate; 5. a longitudinal separator plate; 6. a filtering basin; 7. a transfer mechanism; 8. a storage hopper; 9. a connecting plate; 10. connecting ropes; 11. a temperature control mechanism; 12. a fixed block; 13. a limiting rod; 14. filtering the plate; 15. a discharge pipe; 16. a first sealing door; 17. a second sealing door; 18. a transparent viewing window.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides a technical solution: a filtration auxiliary structure of a cell culture container comprises a filter box 1 and a culture dish 2 arranged inside the filter box 1, wherein the bottoms of the left side and the right side of the filter box 1 are fixedly connected with fixed blocks 12, the left side and the right side of the inner cavity of the filter box 1 are fixedly connected with limit rods 13 between a connecting plate 9 and a connecting rope 10, the left side and the right side of the front top of the filter box 1 are hinged with first sealing doors 16 through hinges, the front bottom of the filter box 1 is hinged with second sealing doors 17 through hinges, the surface of each first sealing door 16 is provided with a transparent observation window 18, the fixed blocks 12, the limit rods 13, the first sealing doors 16, the second sealing doors 17 and the transparent observation windows 18 are matched, the connecting rope 10 is fixed by the fixed blocks 12, the position of a storage hopper 8 is limited through the limit rods 13, and the materials in a feeding pipe 3 can completely fall into the storage hopper 8, meanwhile, a transparent observation window 18 is arranged on the sealing door, which is convenient for experimenters to observe and can be more conveniently developed by using experiments, the left side and the right side of the top of the filter box 1 are both connected with a feeding pipe 3, a transverse isolation plate 4 is fixedly connected between the left side and the right side of the inner cavity of the filter box 1, a longitudinal isolation plate 5 is fixedly connected between the middle part of the top and the bottom of the transverse isolation plate 4 and the top and the bottom of the inner cavity of the filter box 1, a temperature control mechanism 11 is fixedly connected on the surface of the longitudinal isolation plate 5 and above the transverse isolation plate 4, the temperature control mechanism 11 is electrically connected with an external power supply and is controlled by a control switch, a temperature sensor and an intelligent temperature controller are arranged in the temperature control mechanism 11, the temperature sensor and the intelligent temperature controller are mutually and electrically connected, the temperature sensor can detect the temperature in the temperature regulating box in, the proper temperature for maintaining vigorous growth of cultured cells in cell culture is kept, the top of the transverse partition plate 4 and the left and right sides of the longitudinal partition plate 5 are both provided with a filtering basin 6, the inner surface of the filtering basin 6 is provided with a filtering plate 14, the filtering plate 14 is made by wrapping the filtering plate with a filtering membrane (as shown in figure 3), the bottom of the filtering basin 6 is communicated with a discharging pipe 15, the culture dish 2 is arranged below the discharging pipe 15, the left and right sides of the inner cavity of the filtering box 1 are both fixedly connected with a transfer mechanism 7, the transfer mechanism 7 comprises a storage hopper 8, a connecting plate 9 and a connecting rope 10, the bottom of the storage hopper 8 is rotatably connected with the inner surface of the connecting plate 9 through a rotating rod, one side of the storage hopper 8 close to the inner wall of the filtering box 1 is fixedly connected with one end of the connecting rope 10 through the connecting block, and the other end of the, one side of connecting plate 9 and rose box 1's inner wall fixed connection, cooperation rose box 1, culture dish 2, inlet pipe 3, horizontal division board 4, vertical division board 5, filter basin 6, transfer mechanism 7, deposit and fight 8, the setting of connecting plate 9 and connecting rope 10, utilize horizontal division board 4 and vertical division board 5 to divide into four studios with rose box 1, develop simultaneously for two filter works of rose box 1 and provide the condition, be convenient for set up the comparative example, make the test result have the convincing more, and at rose box 1 inside transfer mechanism 7 that sets up, be used for depositing the preheating to the nutrient medium of interpolation, two filter chambers in to rose box 1 carry out the temperature regulation and control through built-in temperature control mechanism 11, the utilization temperature control mechanism 11 of bigger ization.

And those not described in detail in this specification are well within the skill of those in the art.

During the use, start temperature control mechanism 11, after guaranteeing the inside temperature of rose box 1, through inlet pipe 3 to the inside nutrient medium that adds of rose box 1, nutrient medium falls and deposits and fight 8, preheat, when the inside temperature of rose box 1 is constant, loosen connection rope 10 on fixed block 12, hold connection rope 10 and will deposit and fight 8 slow empting on filtering basin 6, pour into in filtering basin 6 completely, taut connection rope 10, make and deposit and fight 8 laminating gag lever post 13, then tie connection rope 10 on fixed block 12, nutrient medium is after the filtration of filter plate 14, directly fall in culture dish 2 through discharging pipe 15, accomplish once filtering promptly.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cell culture container's filtration auxiliary structure, includes rose box (1) and sets up at inside culture dish (2) of rose box (1), its characterized in that: the filter box is characterized in that the left side and the right side of the top of the filter box (1) are both connected with the inlet pipes (3), a transverse partition plate (4) is fixedly connected between the left side and the right side of the inner cavity of the filter box (1), the middle parts of the top and the bottom of the transverse partition plate (4) and the top and the bottom of the inner cavity of the filter box (1) are fixedly connected with a longitudinal partition plate (5), the top of the transverse partition plate (4) and the left side and the right side of the longitudinal partition plate (5) are both provided with a filter basin (6), the left side and the right side of the inner cavity of the filter box (1) are both fixedly connected with a transfer mechanism (7), the transfer mechanism (7) comprises a storage hopper (8), a connecting plate (9) and a connecting rope (10), the bottom of the storage hopper (8) is rotatably connected with the inner surface of the connecting plate (9) through a rotating rod, one side, close to the, the other end of the connecting rope (10) penetrates through the filter box (1) and extends to the outside of the filter box (1), and one side of the connecting plate (9) is fixedly connected with the inner wall of the filter box (1).

2. A filtration assistance structure for a cell culture vessel according to claim 1 wherein: and a temperature control mechanism (11) is fixedly connected to the surface of the longitudinal isolation plate (5) and above the transverse isolation plate (4).

3. A filtration assistance structure for a cell culture vessel according to claim 1 wherein: the equal fixedly connected with fixed block (12) in bottom of rose box (1) left and right sides, the left and right sides of rose box (1) inner chamber just is located equal fixedly connected with gag lever post (13) between connecting plate (9) and connection rope (10).

4. A filtration assistance structure for a cell culture vessel according to claim 1 wherein: the internal surface of filtering basin (6) is provided with filter plate (14), the bottom intercommunication of filtering basin (6) has discharging pipe (15), and culture dish (2) set up the below at discharging pipe (15).

5. A filtration assistance structure for a cell culture vessel according to claim 1 wherein: the left and right sides at positive top of rose box (1) all articulates through the hinge has first sealing door (16), the positive bottom of rose box (1) articulates through the hinge has second sealing door (17).

6. A cell culture vessel filtration assisting structure according to claim 5, wherein: the surface of the first sealing door (16) is provided with a transparent observation window (18).