CN212276099U

CN212276099U - Extremely-simple laser microscope based on three-dimensional specimen lens integrated body

Info

Publication number: CN212276099U
Application number: CN202020994428.3U
Authority: CN
Inventors: 沈天童
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-03
Filing date: 2020-06-03
Publication date: 2021-01-01
Anticipated expiration: 2030-06-03

Abstract

The utility model discloses a very brief formula laser microscope based on three-dimensional sample lens integraph, including three-dimensional sample lens integraph, three-dimensional sample lens integraph includes spherical transparent body and sample, and the sample is fixed and is placed the central authorities of spherical transparent body in spherical transparent body surface or in. The laser microscope has two functions of fixed preservation of a three-dimensional biological specimen and three-dimensional microscopic magnification observation of the specimen, and has the advantages of ingenious design, ultra-small volume, no need of focusing in use, convenient use and display and extremely low manufacturing cost.

Description

Extremely-simple laser microscope based on three-dimensional specimen lens integrated body

Technical Field

The utility model relates to an education and experiment detect technical field, concretely relates to very brief formula laser microscope based on three-dimensional sample lens integraph, this laser microscope is fit for fixed the saving of three-dimensional biological sample, three-dimensional microscopic magnification observation to and the many people of sample are micro-observed simultaneously.

Background

The microscope commonly used at present is an optical microscope, and the device has the defects of complex structure, heavy size, complex operation, easy damage and the like. In the process of observing a biological specimen by using an optical microscope, a series of complex operations such as precise optical focusing, adjustment of light entering conditions of the microscope, adjustment of observation visual field and the like are required, and the requirement on the technical level of observers is high. In addition, the optical microscope commonly used at present has no function for simultaneous observation of multiple persons. The functional design of a common optical microscope is usually only used for single observation, and is inconvenient for simultaneous observation of multiple people such as teaching, meeting, exhibition and the like. The existing optical microscope can realize simultaneous observation of multiple persons only under the condition that a batch of auxiliary equipment such as a camera, a projector and the like are additionally arranged.

On the other hand, all biological specimens observed by the optical microscope are microslide specimens. The microscopic slide specimen is prepared by pressing a biological specimen on a glass slide by means of smear, tabletting, slicing and the like, and is sealed to prepare a sealed planar specimen. Because the operations such as tabletting and slicing are carried out in the manufacturing process of the microscopic slide specimen, the microscopic slide specimen can only carry out planar two-dimensional observation on the biological specimen, cannot realize the omnibearing and 360-degree three-dimensional observation on the biological specimen under a microscope, and has great limitation.

Disclosure of Invention

In order to solve two big limitations that above-mentioned prior art exists simultaneously, the utility model provides a very brief formula laser microscope based on three-dimensional sample lens integration body, this laser microscope simple structure, design benefit, the volume is super little, need not the focusing in the use, and it is convenient to use and show, and the cost of manufacture is extremely cheap.

Realize the utility model discloses the technical scheme that above-mentioned purpose adopted does:

a very simple laser microscope based on a three-dimensional specimen lens integrated body comprises the three-dimensional specimen lens integrated body, wherein the three-dimensional specimen lens integrated body comprises a spherical transparent body and a specimen, and the specimen is fixed on the surface of the spherical transparent body or in the center of the spherical transparent body.

The diameter of the spherical transparent body is 1.0-15mm, the diameter tolerance is less than or equal to 0.05mm, and the light transmittance is more than or equal to 96%.

The spherical transparent body is a spherical lens, and the specimen is permanently adhered to the surface of the spherical transparent body through high-transparency glue.

The spherical transparent body is a spherical lens, and the specimen is temporarily adhered to the surface of the spherical transparent body through water or cedar oil.

The spherical transparent body is a spherical lens, the spherical transparent body comprises two hemispherical lenses, the specimen is adhered to the center of the semicircular surface of one hemispherical lens through high-transmittance glue, and the semicircular surface of the other hemispherical lens is adhered to the semicircular surface of one hemispherical lens.

The spherical transparent body is formed by curing high-transparency glue, and the specimen is wrapped in the center of the spherical transparent body.

Still include the three-dimensional objective table, the three-dimensional objective table includes elastic fixture and adjustment handle pole, and elastic fixture is the breach ring form, and the internal diameter of elastic fixture is the same with the diameter of spherical transparent body, and the adjustment handle pole is located outside the elastic fixture, and the one end of adjustment handle pole is close to breach department with the elastic fixture and is connected, and the adjustment handle pole is located the radial of elastic fixture, and the elastic fixture cover is located on the spherical transparent body.

The three-dimensional object stage also comprises a connecting cylinder and an adjusting mechanism, the elastic clamp is movably connected with the connecting cylinder through the adjusting mechanism, and the elastic clamp can rotate or move along the radial direction of the connecting cylinder.

The adjusting mechanism comprises two adjusting round rods, the two adjusting round rods are located on the outer side of the elastic clamp, one ends of the two adjusting round rods are connected with the elastic clamp, the two adjusting round rods are located on the same radial direction of the elastic clamp, one of the adjusting round rods and the adjusting handle are located on two sides of a notch of the elastic clamp respectively, one end of the connecting cylinder is provided with clamping grooves along the axial symmetry of the connecting cylinder, and the two adjusting round rods are connected with the two clamping grooves in a clamped mode respectively.

Still include miniature laser lamp, miniature laser lamp is cylindricly, is equipped with the external screw thread on the miniature laser lamp, and the other end of connecting cylinder is equipped with the internal thread, miniature laser lamp and connecting cylinder threaded connection, and the sample is towards miniature laser lamp, and the centre of sphere of the light beam of miniature laser lamp, sample and spherical transparent body is located same straight line, and the sample is located the axis of connecting cylinder.

Compared with the prior art, the beneficial effects and advantages of the utility model reside in that:

1. aiming at the defect that the three-dimensional observation of the biological specimen cannot be realized in the traditional optical microscope and microscope slide specimen preparation, the preparation technology of the biological specimen is comprehensively improved, and the laser microscope not only can realize the fixed preservation of the biological specimen, but also can carry out 360-degree three-dimensional microscopic amplification and observation projection on the biological specimen.

2. Compared with a conventional microscope, the microscope can be observed without focusing in the using process, is ingenious in design, ultra-small in size, convenient to carry, extremely convenient to operate and observe, easy to manufacture, low in manufacturing cost and capable of being popularized and applied in a large area.

3. The device is suitable for simultaneous observation of multiple persons, can be used for various observation conditions such as teaching and experiment, and has good microscopic amplification effect.

Drawings

Fig. 1 is an exploded view of a very simplified laser microscope based on a three-dimensional specimen lens assembly according to example 1.

Fig. 2 is a schematic structural view of the three-dimensional stage in example 1.

FIG. 3 is an assembly view of the three-dimensional stage and three-dimensional specimen lens assembly according to example 1.

Fig. 4 is a schematic structural view of a three-dimensional specimen lens integrated body in example 1.

Fig. 5 is a schematic structural view of a three-dimensional specimen lens integrated body in example 3.

Fig. 6 is a schematic structural view of a three-dimensional specimen lens integrated body in example 4.

Fig. 7 is a schematic structural view of a three-dimensional specimen lens integrated body in example 5.

Wherein, 1-three-dimensional specimen lens integrated body: 11-spherical transparent body, 12-specimen, 13-photosensitive glue, 14-hemispherical lens and 15-cover glass; 2-three-dimensional stage: 21-elastic clamp, 22-adjusting handle rod, 23-connecting cylinder, 24-adjusting round rod and 25-clamping groove; 3-micro laser lamp.

Detailed Description

The present invention will be described in detail with reference to the following examples and drawings.

Example 1

The structure of the very simplified laser microscope based on the three-dimensional specimen lens integrated body provided by the embodiment is shown in fig. 1, and includes a three-dimensional specimen lens integrated body 1, a three-dimensional stage 2 and a micro laser lamp 3.

The three-dimensional specimen lens integrated body 1 comprises a spherical transparent body 11 and a specimen 12, wherein the spherical transparent body 11 is a spherical lens, the diameter is 1-15mm, the diameter tolerance is less than or equal to 0.05mm, and the light transmittance is more than or equal to 96%. In this embodiment, the diameter of the spherical lens is 6.0mm, the transmittance is 98%, and the diameter tolerance is 0.01 mm. The spherical lens is made of glass; the specimen 12 includes solid samples such as animal, plant specimens, and natural materials. As shown in fig. 4, the specimen 12 is permanently adhered to the surface of the spherical lens by a high-transparency glue to form a three-dimensional specimen lens integrated body 1, wherein the high-transparency glue can be a photosensitive glue, a neutral glue, an epoxy resin or a thermosol, and in this embodiment, the high-transparency glue is a photosensitive glue (ergo 8500 type photosensitive glue, switzerland).

As shown in fig. 2, the three-dimensional stage includes an elastic clamp 21, an adjustment handle bar 22, a connecting cylinder 23 and two adjustment round bars 24. One end of the connecting cylinder 23 is symmetrically provided with a clamping groove 25 along the axial direction, and the other end of the connecting cylinder is provided with internal threads, wherein in the embodiment, the length of the connecting cylinder 23 is 2.4cm, and the outer diameter is 1.6 cm.

The elastic clamp 21 is in a shape of a notched ring, and in this embodiment, the material of the elastic clamp 21 may be metal or plastic. The adjusting handle rod 22 and the two adjusting round rods 24 are both positioned outside the elastic clamp, one end of each adjusting handle rod 22 and one end of each adjusting round rod 24 are respectively connected with the elastic clamp 21, and one adjusting round rod 24 and one adjusting handle rod 22 are respectively positioned on two sides of a gap of the elastic clamp 21. The adjusting handle rod 22 and each adjusting round rod 24 are respectively positioned in the radial direction of the elastic clamp 21, and the two adjusting round rods 24 are positioned in the same radial direction of the elastic clamp 21. The size of the inner diameter of the elastic clamp 21 can be adjusted by digging the adjusting handle rod 22, so that the spherical transparent body can be clamped and placed.

As shown in fig. 3, the elastic clamp 21 is sleeved on the spherical transparent body 11, and the two adjusting rods 24 are respectively clamped with the two clamping grooves 25.

The miniature laser lamp 3 is cylindrical, the model of the miniature laser lamp is Oxlasers OX-1200, the power is 100mW, the wavelength is 532nm, and the color of the emitted laser is green. In this embodiment, the length of the micro laser 3 is 11.3cm and the diameter is 1.3 cm. Be equipped with the external screw thread on the miniature laser lamp 3, miniature laser lamp 3 and connecting cylinder 23 threaded connection. The specimen 12 faces the miniature laser lamp 3, the light beam of the miniature laser lamp 3, the specimen 12 and the spherical center of the spherical transparent body 11 are positioned on the same straight line, and the specimen 12 is positioned on the axis of the connecting cylinder 23.

After the three-dimensional specimen lens integrated body 1, the three-dimensional objective table 2 and the miniature laser lamp 3 are assembled together, the whole microscope is in a stepped cylinder shape, the length is 12.4cm, and the diameter is not more than 1.6 cm. The microscope after the assembly is ultra-small in overall size and convenient to carry and demonstrate.

The manufacturing and using method of the ultra-simple laser microscope based on the three-dimensional specimen lens integrated body is as follows:

1. manufacturing a three-dimensional specimen lens integrated body:

taking a glass spherical lens with the diameter of 6.0mm, cleaning the spherical lens with purified water, drying the glass spherical lens for later use, clamping the clean dust-free spherical lens by using an elastic clamp of a three-dimensional objective table, placing the spherical lens on a clean operation table, dripping a photosensitive adhesive liquid drop on the center of the top surface of the spherical lens, keeping the photosensitive adhesive liquid drop on the surface of the spherical lens as regular as possible, picking a specimen by using a needle, sealing and immersing the specimen into the photosensitive adhesive liquid drop, paying attention to avoid mixing bubbles and dust, irradiating the specimen for more than 60s by using an ultraviolet lamp, then placing the specimen in a clean dust-free environment, standing the specimen for more than 7 hours until the photosensitive adhesive is completely cured, namely manufacturing a three-dimensional specimen lens integrated body in a permanent bonding mode, and placing the integrated body in a clean and dry container for later use;

2. assembling the three-dimensional specimen lens integrated body:

clamping a spherical lens by using an elastic clamp, clamping and placing by operating an adjusting handle rod, respectively clamping two adjusting round rods in two clamping grooves of a connecting cylinder, then installing a miniature laser lamp on the connecting cylinder, moving the two adjusting round rods up and down to adjust the position of a three-dimensional specimen lens integrated body, so that the spherical center of the spherical lens is positioned on the axis of the connecting cylinder, rotating the two adjusting round rods, and paying attention to enable one side of a specimen of the three-dimensional specimen lens integrated body to face and be opposite to the miniature laser lamp, and the light beam of the miniature laser lamp, the specimen and the spherical center of a spherical transparent body are accurately positioned on the same straight line;

3. three-dimensional microscopic observation of the specimen:

the method comprises the steps of finding a white wall or a piece of white paper to face a three-dimensional specimen lens integrated body to serve as a display screen, turning on a switch of a miniature microscope, enabling a laser beam to sequentially penetrate through the centers of a specimen and a spherical ball lens, projecting an amplified sample on the white wall or the white paper, adjusting the distance and the angle between the microscope and the white wall or the white paper to adjust the amplification effect of the microscope, and moving or rotating the spherical lens through two adjusting round rods to move or rotate the specimen so as to realize 360-degree omnibearing and three-dimensional microscopic amplification observation of the specimen. Meanwhile, the microscopic observation purpose and the best effect are achieved by adjusting the observation visual field.

4. Disassembling and maintaining the three-dimensional specimen lens integrated body:

after the observation is finished, the switch of the miniature laser lamp is closed, the three-dimensional objective table is detached, a clean glass plate is found, the three-dimensional specimen lens integrated body is detached and placed into the glass plate, the three-dimensional specimen lens integrated body is placed in a dry light-shading container as a permanent specimen for storage, and the next observation is reserved. When dust sticks on the three-dimensional specimen lens integrated body and the microscopic observation effect is influenced, the three-dimensional specimen lens integrated body is washed by ethanol and purified water in sequence, and then carefully wiped and cleaned by using a fine silk cloth.

Example 2

The difference from example 1 is that the bonding method of the specimen 12 and the spherical lens (spherical transparent body 11) in the three-dimensional specimen lens integrated body is different, and in this example, the specimen 12 is temporarily bonded to the spherical lens, and the manufacturing, storage and maintenance of the three-dimensional specimen lens integrated body are different due to the different bonding method.

The manufacturing method of the three-dimensional specimen lens integrated body comprises the following steps:

taking a glass spherical lens with the diameter of 6.0mm, cleaning the spherical lens with purified water, drying the spherical lens for later use, clamping the clean dust-free spherical lens by using an elastic clamp, placing the spherical lens on a clean operating platform, dripping water drops on the center of the top surface of the spherical lens by using a needle or an injector, picking a specimen by using the needle, and hermetically immersing the specimen into the water drops;

the preservation and maintenance method of the three-dimensional specimen lens integrated body comprises the following steps:

after the observation is finished, the switch of the miniature laser lamp is turned off, the three-dimensional objective table is detached, the sample on the spherical lens is washed by water, a clean glass plate is found, the spherical lens is placed in the glass plate, and the sample is stored in a dry mode.

Example 3

The difference from example 1 is that the structure of the three-dimensional specimen lens assembly is different, and as shown in fig. 5, a cover glass 15 is covered on a specimen 12 in the present example, and the method for manufacturing the three-dimensional specimen lens assembly in the present example is as follows:

taking a glass spherical lens with the diameter of 6.0mm, cleaning the spherical lens with purified water, drying the spherical lens for later use, clamping the clean dust-free spherical lens by using an elastic clamp of a three-dimensional objective table, placing the spherical lens on a clean operation table, dripping a photosensitive adhesive liquid drop on the center of the top surface of the spherical lens, keeping the photosensitive adhesive liquid drop on the surface of the spherical lens as regular as possible, picking a specimen by using a needle, sealing and immersing the specimen into the photosensitive adhesive liquid drop, carefully placing a circular cover glass with the diameter of 3mm and the thickness of 0.17mm on the specimen, paying attention to avoid generating bubbles and bringing impurities, irradiating the specimen by using an ultraviolet lamp for more than 60 seconds, then placing the specimen in a clean dust-free environment for more than 7 hours until the photosensitive adhesive is completely cured, namely manufacturing a specimen three-dimensional lens integrated body, and placing the specimen integrated body in a clean and dry container for later use.

Example 4

The difference from example 1 is that the structure of the three-dimensional specimen lens integrated body of the present example is: as shown in fig. 6, the spherical transparent body includes two hemispherical lenses 14, the specimen 12 is adhered to the center of the semicircular surface of one of the hemispherical lenses 14 by a photosensitive adhesive 13, and the semicircular surface of the other hemispherical lens 14 is adhered to the semicircular surface of one of the hemispherical lenses 14.

The method for manufacturing the three-dimensional specimen lens integrated body of the present embodiment is as follows:

taking two glass hemispherical lenses with the diameter of 6.0mm, cleaning the two hemispherical lenses with purified water, drying the two hemispherical lenses for later use, clamping one hemispherical lens by an elastic clamp of a three-dimensional objective table, placing the hemispherical lens on a clean operating table, coating photosensitive adhesive on the semicircular surface of the hemispherical lens, picking a specimen by using a needle, sealing and immersing the specimen in the center of the semicircular surface of the hemispherical lens, paying attention to avoid bubbles and carrying impurities, placing the circular surface of the other hemispherical lens on the semicircular surface of the hemispherical lens, irradiating the surface for more than 60 seconds by using an ultraviolet lamp, then placing the hemispherical lens in a clean and dustless environment, standing the hemispherical lens for more than 7 hours until the photosensitive adhesive is completely cured, namely manufacturing a three-dimensional specimen lens integrated body, and placing the three-dimensional specimen lens integrated body in a clean and dry container for later use.

Example 5

The difference from the embodiment 1 lies in the material of the spherical transparent body 11 and the position of the specimen, as shown in fig. 7, the spherical transparent body 11 of the embodiment is formed by curing a photosensitive adhesive, and the specimen 12 is wrapped in the center of the spherical transparent body 11.

under clean dustless environment, absorb the photosensitive adhesive with clean syringe, put the syringe perpendicularly downwards, promote the syringe carefully, make syringe exit form a spherical liquid drop, reuse the needle and pick the sample, place the sample carefully inside the spherical liquid drop, take care of avoiding producing the bubble and bringing into impurity, irradiate more than 60s with the ultraviolet lamp subsequently, place and stand still more than 7 hours in clean dustless environment until the photosensitive adhesive is totally solidified, make into three-dimensional sample lens integument promptly, place and keep in clean dry container for subsequent use.

Claims

1. A very brief laser microscope based on three-dimensional sample lens integrant which characterized in that: the three-dimensional specimen lens integrated body comprises a spherical transparent body and a specimen, and the specimen is fixed on the surface of the spherical transparent body or in the center of the spherical transparent body.

2. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: the diameter of the spherical transparent body is 1.0-15mm, the diameter tolerance is less than or equal to 0.05mm, and the light transmittance is more than or equal to 96%.

3. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: the spherical transparent body is a spherical lens, and the specimen is permanently adhered to the surface of the spherical transparent body through high-transparency glue.

4. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: the spherical transparent body is a spherical lens, and the specimen is temporarily adhered to the surface of the spherical transparent body through water or cedar oil.

5. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: the spherical transparent body is a spherical lens, the spherical transparent body comprises two hemispherical lenses, the specimen is adhered to the center of the semicircular surface of one hemispherical lens through high-transmittance glue, and the semicircular surface of the other hemispherical lens is adhered to the semicircular surface of one hemispherical lens.

6. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: the spherical transparent body is formed by curing high-transparency glue, and the specimen is wrapped in the center of the spherical transparent body.

7. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: still include the three-dimensional objective table, the three-dimensional objective table includes elastic fixture, adjustment handle pole, and elastic fixture is the breach ring form, and the internal diameter of elastic fixture is the same with the diameter of spherical transparent body, and adjustment handle pole is located outside the elastic fixture, and the one end of adjustment handle pole is close to breach department with the elastic fixture and is connected, and just the adjustment handle pole is located the radial of elastic fixture, and the elastic fixture cover is located on the spherical transparent body.

8. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 7, wherein: the three-dimensional object stage also comprises a connecting cylinder and an adjusting mechanism, the elastic clamp is movably connected with the connecting cylinder through the adjusting mechanism, and the elastic clamp can rotate or move along the radial direction of the connecting cylinder.

9. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 8, wherein: the adjusting mechanism comprises two adjusting round rods, the two adjusting round rods are located on the outer side of the elastic clamp, one ends of the two adjusting round rods are connected with the elastic clamp, the two adjusting round rods are located on the same radial direction of the elastic clamp, one of the adjusting round rods and the adjusting handle are located on two sides of a notch of the elastic clamp respectively, one end of the connecting cylinder is provided with clamping grooves along the axial symmetry of the connecting cylinder, and the two adjusting round rods are connected with the two clamping grooves in a clamped mode respectively.

10. The three-dimensional specimen lens integrated body-based very simple laser microscope of claim 1, wherein: still include miniature laser lamp, miniature laser lamp is cylindricly, is equipped with the external screw thread on the miniature laser lamp, and the other end of connecting cylinder is equipped with the internal thread, miniature laser lamp and connecting cylinder threaded connection, and the sample is towards miniature laser lamp, and the centre of sphere of the light beam of miniature laser lamp, sample and spherical transparent body is located same straight line, and the sample is located the axis of connecting cylinder.