CN212270130U - Multi-concave cell culture sheet - Google Patents
Multi-concave cell culture sheet Download PDFInfo
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- CN212270130U CN212270130U CN202020224449.7U CN202020224449U CN212270130U CN 212270130 U CN212270130 U CN 212270130U CN 202020224449 U CN202020224449 U CN 202020224449U CN 212270130 U CN212270130 U CN 212270130U
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Abstract
The utility model relates to a many concave surface cell culture piece for place in the cell culture apparatus, many concave surface cell culture piece including cultivate the piece body and a plurality of certainly the concave surface of the inside sunken formation in surface of cultivateing the piece body, the concave surface has the concave surface terminal and is used for increasing the column cavity or the taper cavity of concave surface degree of depth, this column cavity or taper cavity set up in the terminal top of concave surface and with concave surface end-to-end connection. The cell culture sheet with multiple concave surfaces is arranged into a culture sheet with a plurality of concave surfaces, and each concave surface is provided with a smooth concave surface tail end so as to obtain a large number of cell balls; placing the multi-concave cell culture sheet into a cell culture tool, so that a large number of cell balls can be obtained in a single hole of the cell culture tool; the multi-concave cell culture sheet is formed by integral injection molding, has low cost and simple structure, is convenient and quick to use, and has the advantage of high flux.
Description
Technical Field
The utility model relates to a many concave surfaces cell culture piece.
Background
The conventional cell culture is an adherent two-dimensional culture mode, but because the communication among cells is very little, cell groups cannot have the structure and physiological and biochemical characteristics similar to those in vivo; for example, liver cells lose the hepatic ductuli and function of secreting albumin/urea several days later in adherent culture, or cancer cells lack biomarkers in vivo in two-dimensional culture. In contrast, the three-dimensional culture of the cells can effectively improve and simulate the in vivo environment, for example, the three-dimensional culture of the hepatocytes has the three-dimensional structure and the liver function of a natural liver, and more accurate hepatotoxicity data is obtained; the cancer cells can reproduce some biomarkers and certain metastasis and infiltration functions under three-dimensional culture. Therefore, the importance of three-dimensional cell culture is self evident.
However, the current three-dimensional culture method is too complex and high in economic cost (such as a scaffold method), so that the three-dimensional culture method cannot be widely accepted by common researchers. Or lack of high throughput features, such as the use of agarose or ultra-low adsorption plates in 96-well plates, where only one cell three-dimensional sphere can be formed in one well; since each cell sphere has only about 1000 cells, it cannot meet the standards of protein detection, qPCR quantification of mRNA, cell sphere staining, and the lowest cell number of most kits (typically, fifty thousand cells are needed to obtain more reliable data). Therefore, in one cell culture hole, a plurality of cell balls can be obtained simply and rapidly, and the three-dimensional cell culture can be favored by researchers.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a many concave surfaces cell culture piece, its simple structure and convenient operation.
In order to achieve the above object, the utility model provides a following technical scheme a many concave surface cell culture piece for place in the cell culture apparatus, a serial communication port, many concave surface cell culture piece is including cultivateing piece body and a plurality of certainly the concave surface of the inside sunken formation in surface of cultivateing piece body.
Further, the concave surface has a concave end, and the shape of the cross section of the concave end is arc-shaped or rectangular or conical.
Further, the diameter of the concave end ranges from 0.4 mm to 5mm, and the depth of the concave end ranges from 0.2 mm to 2.5 mm.
Furthermore, the concave surface also comprises a cylindrical cavity or a conical cavity for increasing the depth of the concave surface, and in the height direction of the multi-concave surface cell culture sheet, the tail end of the concave surface is positioned below the cylindrical cavity or the conical cavity and is connected with the cylindrical cavity or the conical cavity.
Further, the depth range of the columnar cavity or the conical cavity is 0-2 mm.
Further, the multi-concave cell culture sheet is formed by integral injection molding, and the smoothness of the multi-concave cell culture sheet is nano-scale.
The beneficial effects of the utility model reside in that: the multi-concave cell culture sheet is provided with a plurality of concave surfaces, and each concave surface is provided with a concave surface tail end so as to obtain cell balls; placing the multi-concave cell culture sheet into a cell culture tool, so that a large number of cell balls can be obtained in a single hole of the cell culture tool;
the multi-concave cell culture sheet is formed by integral injection molding, has low cost and simple structure, is convenient and quick to use, and has the advantage of high flux.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
FIG. 1 is a schematic structural view of the multi-concave cell culture sheet of the present invention.
FIG. 2 is a schematic cross-sectional view of the multi-concave cell culture sheet of the present invention.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Referring to fig. 1 and 2, the multi-concave cell culture sheet according to a preferred embodiment of the present invention can be placed in a cell culture tool. The cell culture tool is any one of a 24-well plate, a 12-well plate, a 6-well plate, and a 35mm cell culture dish and a 60mm cell culture dish, but in other embodiments, the cell culture tool may be other, and is not particularly limited herein according to the actual situation. The diameter of the multi-concave cell culture sheet is determined according to the specification of the cell culture tool, and for example, when the cell culture tool is a 6-well plate, the diameter of the multi-concave cell culture sheet is in the range of 30-33.3mm, preferably 33.3 mm; when the cell culture tool is a 24-well plate, the diameter of the multi-concave cell culture sheet is in the range of 13-14.3mm, preferably 14.3 mm. Indeed, in other embodiments, the diameter of the multi-concave cell culture sheet may be other, and is not limited herein, depending on the actual situation.
The multi-concave cell culture sheet comprises a culture sheet body 1 and a plurality of concave surfaces 2 formed by inwards sinking from the surface of the culture sheet body 1, wherein each concave surface 2 is provided with a concave end 21. The cross section of the concave end 21 is arc-shaped, rectangular, conical or other shapes, which are not limited herein, according to the actual situation. The concave end 21 is most effective in forming a cell sphere when it has an arc shape in cross section. The diameter range of the concave end 21 is 0.4-5mm, and the depth range of the concave end 21 is 0.2-2.5 mm. Indeed, in other embodiments, the diameter range and the depth range of the concave end 21 may be other, and are not limited specifically herein, depending on the actual situation. The concave surface 2 further comprises a cylindrical cavity 22 or a conical cavity 22 for increasing the depth of the concave surface 2, and in the height direction of the multi-concave cell culture sheet, the end of the concave surface 21 is located below the cylindrical cavity 22 or the conical cavity 22 and is connected with the cylindrical cavity 22 or the conical cavity 22. The depth range of the cylindrical cavity 22 or the conical cavity 22 is 0-2mm, that is, the cylindrical cavity 22 or the conical cavity 22 may be provided or not provided, which is determined according to actual requirements. The cylindrical cavity 22 or the conical cavity may be a cavity of other shapes, and is not particularly limited herein, depending on the actual situation.
The multi-concave cell culture sheet is integrally formed by injection molding, so that the method is fast, simple and convenient, the manufacturing process is simplified, and the production cost is reduced. The smoothness of the multi-concave cell culture sheet is nano-scale, so that the adsorbability of cells on the surface of the multi-concave cell culture sheet is greatly reduced, the cells can smoothly slide to the concave end 21, and the efficiency and the quality of cell balling are improved.
The utility model also provides a method for culturing three-dimensional cell balls, adopts the multi-concave cell culture sheet, the method comprises:
placing the multi-concave cell culture sheet in a cell culture tool;
adding an ethanol solution into the cell culture tool to perform infiltration and sterilization treatment on the multi-concave cell culture sheet;
after the ethanol solution is absorbed, adding a phosphate buffer solution, and repeating the operation once;
adding a cell culture medium to enable the cell culture medium to infiltrate the multi-concave cell culture sheet, and reserving 0.2-2ml of the cell culture medium;
the cell suspension is added to the cell culture tool and the cells settle by gravity in the concave surface to form a cell pellet.
Wherein the step of adding an ethanol solution into the cell culture tool to infiltrate and sterilize the multi-concave cell culture sheet specifically comprises the following steps:
adding an ethanol solution into the cell culture tool, so that the ethanol solution submerges the multi-concave cell culture sheet, and keeping for 15 minutes so that the ethanol solution infiltrates and sterilizes the multi-concave cell culture sheet. Indeed, in other embodiments, other solutions may be used for the soaking and sterilization effects, which are determined according to the actual situation and are not specifically limited herein.
The concentration range of the ethanol solution is 75-100%, and the volume range of the ethanol solution is 0.2-2 ml. Indeed, in other embodiments, the concentration and volume of the ethanol may be other, and are not specifically limited herein, depending on the actual situation. The purpose of using the ethanol solution is: the concavity 21 may be both sterilized and infiltrated to remove air bubbles that may be present in the concavity 21 to prevent affecting cell balling.
In summary, the following steps: the multi-concave cell culture sheet is provided with a plurality of concave surfaces 2, and each concave surface 2 is provided with a concave surface tail end to obtain a cell ball; placing the multi-concave cell culture sheet into a cell culture tool, so that a large number of cell balls can be obtained in a single hole of the cell culture tool;
the multi-concave cell culture sheet is formed by integral injection molding, has low cost and simple structure, is convenient and quick to use, and has the advantage of high flux.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (5)
1. A multi-concave cell culture sheet is used for being placed in a cell culture tool and is characterized by comprising a culture sheet body and a plurality of concave surfaces formed by inwards recessing the surface of the culture sheet body; the concave surface has a concave end, and the cross section of the concave end is arc-shaped or rectangular or conical.
2. The multiconcave cell culture sheet according to claim 1, wherein the diameter of the concave tip ranges from 0.4 to 5mm and the depth of the concave tip ranges from 0.2 to 2.5 mm.
3. The multi-concave cell culture plate according to claim 1, wherein the concave surface further comprises a cylindrical cavity or a conical cavity for increasing the depth of the concave surface, and the end of the concave surface is located below the cylindrical cavity or the conical cavity and connected with the cylindrical cavity or the conical cavity in the height direction of the multi-concave cell culture plate.
4. The multiconcave cell culture sheet according to claim 3, wherein the depth of the columnar or conical cavities is in the range of 0 to 2 mm.
5. The multi-concave cell culture sheet according to claim 1, wherein the multi-concave cell culture sheet is formed by injection molding, and the smoothness of the multi-concave cell culture sheet is nano-scale.
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| CN202020224449.7U CN212270130U (en) | 2020-02-28 | 2020-02-28 | Multi-concave cell culture sheet |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154649A (en) * | 2020-02-28 | 2020-05-15 | 苏州大学 | Seal for manufacturing multi-concave gel sheet and using method |
| CN111172035A (en) * | 2020-02-28 | 2020-05-19 | 苏州大学 | Multi-concave cell culture sheet and culture method of three-dimensional cell spheres |
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2020
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154649A (en) * | 2020-02-28 | 2020-05-15 | 苏州大学 | Seal for manufacturing multi-concave gel sheet and using method |
| CN111172035A (en) * | 2020-02-28 | 2020-05-19 | 苏州大学 | Multi-concave cell culture sheet and culture method of three-dimensional cell spheres |
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