CN211807345U - Die device for manufacturing multi-concave cell culture sheet - Google Patents
Die device for manufacturing multi-concave cell culture sheet Download PDFInfo
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- CN211807345U CN211807345U CN202020224460.3U CN202020224460U CN211807345U CN 211807345 U CN211807345 U CN 211807345U CN 202020224460 U CN202020224460 U CN 202020224460U CN 211807345 U CN211807345 U CN 211807345U
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Abstract
The utility model relates to a mold device for making many concave surfaces cell culture piece, mold device includes metal mold, and form in metal mold and with the middle mould of metal mold detachable, middle mould sets up with the metal mold mirror image, be provided with the first recess of forming from the metal mold surface is inside sunken, set up the first arch in first recess and set up a plurality of concave surface on first arch, middle mould be provided with first protruding assorted second recess and a plurality of setting in the second recess and with concave surface assorted second arch. The utility model discloses a mould device for making many concave surfaces cell culture piece, its simple structure and convenient operation for a plurality of concave surface has on the final fashioned cell culture piece, has improved the acquisition volume of the cell ball of every concave surface, thereby for follow-up quantitative test provides sufficient cell volume, under the condition that does not increase cell culture apparatus structure and complicates, improved acquisition rate, convenient and fast.
Description
Technical Field
The utility model relates to a mold device for making many concave surfaces cell culture piece.
Background
The conventional cell culture is an adherent two-dimensional culture mode, but because the communication among cells is very little, the cell group cannot have the structure and physiological and biochemical characteristics similar to those in vivo. For example, liver cells lose the hepatic ductuli and function of secreting albumin/urea several days later in adherent culture, or cancer cells lack biomarkers in vivo in two-dimensional culture. In contrast, the three-dimensional culture of the cells can effectively improve and simulate the in vivo environment, for example, the three-dimensional culture of the hepatocytes has the three-dimensional structure and the liver function of a natural liver, and more accurate hepatotoxicity data is obtained; the cancer cells can reproduce some biomarkers and certain metastasis and infiltration functions under three-dimensional culture. Therefore, the importance of three-dimensional cell culture is self evident.
However, the current three-dimensional culture method is too complex and expensive (such as a scaffold method), so that the three-dimensional culture method cannot be widely accepted by ordinary researchers. Or lack of high throughput features, such as the use of agarose or ultra-low adsorption plates in 96-well plates, where only one cell three-dimensional sphere can be formed in one well; since each cell pellet has only about 1000 cells, the criteria for protein detection, qPCR quantification of mRNA, cell pellet staining, and the lowest cell number of most kits cannot be met.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a mold device for making many concave surfaces cell culture piece, its simple structure and convenient and fast have improved the acquisition rate of cell ball.
In order to achieve the above purpose, the utility model provides a following technical scheme: the utility model provides a mold device for making many concave surfaces cell culture piece, mold device includes the metal mold, and forms in the metal mold in and with the mould in the middle of the metal mold detachable, middle mould with the metal mold mirror image sets up, be provided with on the metal mold certainly the first recess of the inside sunken formation in metal mold surface, setting are in first arch in the first recess and setting are in a plurality of concave surface on the first arch, middle mould be provided with first protruding assorted second recess and a plurality of setting are in the second recess and with concave surface assorted second is protruding.
Further, the concave surface has a concave end, the second protrusion has a top, and the cross sections of the concave end and the top are arc-shaped or rectangular or conical.
Further, the diameter of the concave end and the top is in the range of 0.4-5mm, and the depth of the concave end and the top is in the range of 0.2-2.5 mm.
Further, each concave surface is also provided with a cylindrical cavity or a conical cavity for increasing the depth of the concave surface, and the second bulge is also provided with a cylindrical part or a conical part for connecting the top part.
Further, the diameter of the cylindrical cavity or the conical cavity and the cylindrical part or the conical part ranges from 0.4 mm to 5mm, and the depth of the cylindrical cavity or the conical cavity and the cylindrical part or the conical part ranges from 0 mm to 2.5 mm.
Further, the first protrusion is disposed at the center of the first groove, and the second groove is disposed at the center of the intermediate mold.
Furthermore, at least one lug is arranged in the first groove and is connected with the groove wall of the first groove; the middle mould is also provided with at least one third groove matched with the bump.
Further, the smoothness of the metal mold is on the order of nanometers.
Further, the number of the concave surfaces and the second protrusions is not less than 100.
The beneficial effects of the utility model reside in that: the utility model discloses a mold device for making many concave surfaces cell culture piece, a structure is simple, through be provided with first arch and set up a plurality of concave surface in first arch on metal mold, and mold in the middle of the shaping in the metal mold, middle mould has the second recess and sets up the second arch on the second recess, this second arch and concave surface shape phase-match, make a plurality of concave surface have equally on final fashioned cell culture piece, the acquisition volume of the cell ball of every concave surface has been improved, thereby provide sufficient cell volume for follow-up quantitative test, under the condition that does not increase cell culture apparatus structure and complicates, the acquisition rate has been improved, and is convenient and fast.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
FIG. 1 is a schematic structural diagram of a metal mold in a mold device for manufacturing a multi-concave cell culture sheet according to the present invention.
Fig. 2 is a schematic cross-sectional view of fig. 1.
FIG. 3 is a schematic structural view of the middle mold of the mold device for manufacturing the multi-concave cell culture plate of the present invention
Fig. 4 is a schematic cross-sectional view of the concave surface of fig. 1.
Fig. 5 is another schematic cross-sectional view of the concave surface of fig. 1.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Referring to fig. 1 to 3, in a preferred embodiment of the present invention, a mold device for manufacturing a multi-concave cell culture sheet includes a metal mold 1 and an intermediate mold 2 formed in the metal mold 1 and detachable from the metal mold 1, in this embodiment, the intermediate mold 2 may be formed by curing Polydimethylsiloxane (PDMS) liquid in the metal mold 1, and indeed, in other embodiments, the intermediate mold may be made of other materials as long as the corresponding technical effects are achieved, which is not particularly limited herein, depending on the actual situation. Specifically, the middle mold 2 and the metal mold 1 are arranged in a mirror image manner, wherein the smoothness of the metal mold 1 is in a nanometer level. Be provided with on the metal mold 1 certainly the first recess 11, the setting that the inside sunken formation in metal mold 1 surface are in first arch 12 in the first recess 11 and setting are in a plurality of concave surface 13 on the first arch 12, middle mould 2 be provided with first protruding 12 assorted second recess 21 and a plurality of setting are in the second recess 21 and with concave surface 13 assorted second is protruding 22. In this embodiment, the first protrusion 12 is disposed at the center of the first groove 11, and correspondingly, the second groove 21 is disposed at the center of the middle mold 2. Indeed, in other embodiments, the first protrusion 12 may be disposed at other positions of the first groove 11, and the second groove 21 may be disposed at other positions of the middle mold 2, which is not specifically limited herein, depending on the actual situation.
The concave surfaces 13 are uniformly distributed, the second protrusions 22 are also uniformly distributed, and the number of the concave surfaces 13 and the number of the second protrusions 22 are not less than 100. Indeed, in other embodiments, the plurality of concave surfaces 13 and the plurality of second protrusions 22 may also be arranged unevenly, and are not limited herein, depending on the actual situation. Wherein, each concave 13 has a concave end 131, each second protrusion 22 has a top (not numbered), the diameter range of the concave end 131 and the top is 0.4-5mm, the depth range of the concave end 131 and the top is 0.2-2.5mm, the cross section of the concave end 131 and the top is in the shape of arc, rectangle or cone, preferably arc, the arc is easier to make the cell ball. Indeed, in other embodiments, the diameters and depth ranges of the concave end 131 and the top portion may be other, and the cross-sectional shapes of the concave end 131 and the top portion may also be other, which are not specifically limited herein according to practical situations.
Referring to fig. 4 and 5, each of the concave surfaces 13 further has a cylindrical cavity 132 or a tapered cavity 132 for increasing the depth of the concave surface 13, and the second protrusion 22 further has a cylindrical or tapered part (not shown) for connecting the top, wherein the cylindrical or tapered cavity 132 is disposed above and connected to the concave end 131 in the height direction of the multi-concave cell culture sheet. The diameter of the cylindrical cavity 132 or the conical cavity 132 and the cylindrical member or the conical member ranges from 0.4 mm to 5mm, and the depth of the cylindrical cavity 132 or the conical cavity 132 and the cylindrical member or the conical member ranges from 0 mm to 2.5 mm. However, in other embodiments, the diameter ranges of the cylindrical cavity 132 or the conical cavity 132 and the cylindrical member or the conical member, and the depth ranges of the cylindrical cavity 132 or the conical cavity 132 and the cylindrical member or the conical member may be other, which are not specifically limited herein, depending on the actual situation.
At least one lug 14 is further arranged in the first groove 11, the lug 14 is connected with the groove wall of the first groove 11, and the intermediate mold 2 is further provided with at least one third groove 23 matched with the lug 14. In this embodiment, the number of the bumps 14 and the third grooves 23 is 4, the 4 bumps 14 and the third grooves 23 are uniformly distributed, and the bumps 14 and the third grooves 23 are shaped like half-rugby ball finger surfaces. However, in other embodiments, the number and shape of the protrusions 14 and the third grooves 23 may be other, and are not limited herein.
In the present embodiment, the intermediate mold is formed by pouring Polydimethylsiloxane (PDMS) liquid into the metal mold 1, so that it is cured; because the polydimethylsiloxane is colorless, tasteless, nontoxic, nonflammable and nonvolatile liquid, the polydimethylsiloxane is optically transparent after being cured and has stable physicochemical property, so that the intermediate mold 2 is formed stably. Then pouring the gel solution into the middle mold 2, solidifying the gel solution to obtain a molded cell culture sheet, wherein the cell culture sheet is provided with a plurality of concave surfaces. The final multi-concave cell culture sheet is in conformity with the shape of the convex 12 and concave 13 of the metal mold 1. The intermediate mould 2 is then pushed so that the multiconcave cell culture sheet can be easily extruded from the intermediate mould 2. In this example, the gel solution is an agarose solution. Indeed, in other embodiments, the gel solution may also be a hydrogel solution, which is not specifically limited herein, depending on the actual situation.
The finally molded multi-concave cell culture sheet is placed in a cell culture tool, cell suspension is added, cells can slowly gather in each concave surface 131 of the multi-concave cell culture sheet, and the cells can be formed into balls after three days.
In summary, the following steps: the utility model discloses a mold device for making many concave surface cell culture pieces, a structure is simple, through be provided with first arch 12 and set up a plurality of concave surface 13 on first arch 12 on metal mold 1, but mold 2 in the middle of the shaping in metal mold 1, middle mold 2 has second recess 21 and sets up the protruding 22 of second on second recess 21, this protruding 22 of second and concave surface 13 shape phase-match, make a plurality of concave surface 13 have equally on the last fashioned cell culture piece, the acquisition volume of the cell ball of every concave surface 13 has been improved, thereby provide sufficient cell volume for follow-up quantitative test, under the condition that does not increase cell culture apparatus structure and complicates, the acquisition rate has been improved, and convenience and rapidness.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (9)
1. The utility model provides a mold device for making many concave surfaces cell culture piece, a serial communication port, mold device includes the metal mold, and forms in the metal mold and with mould in the middle of the metal mold detachable, middle mould with the metal mold mirror image sets up, be provided with on the metal mold certainly the first recess of the inside sunken formation in metal mold surface, set up and be in first arch in the first recess and set up a plurality of concave surface on the first arch, middle mould be provided with first protruding assorted second recess and a plurality of setting are in the second recess and with concave surface assorted second is protruding.
2. The mold device for making a multi-concave cell culture sheet according to claim 1, wherein the concave surface has a concave end, the second protrusion has a top, and the cross section of the concave end and the top has an arc shape, a rectangular shape or a conical shape.
3. The mold device for making a multi-concave cell culture plate according to claim 2, wherein the diameter of the concave end and the top is in the range of 0.4-5mm, and the depth of the concave end and the top is in the range of 0.2-2.5 mm.
4. The mold device for making a multi-concave cell culture plate according to claim 2, wherein each concave surface further has a cylindrical cavity or a conical cavity for increasing the depth of the concave surface, and the second protrusion further has a cylindrical member or a conical member for connecting the top portion.
5. The mold device for making a multi-concave cell culture sheet according to claim 4, wherein the diameter of the cylindrical cavity or the conical cavity and the cylindrical member or the conical member ranges from 0.4 mm to 5mm, and the depth of the cylindrical cavity or the conical cavity and the cylindrical member or the conical member ranges from 0 mm to 2.5 mm.
6. The mold device for making a multi-concave cell culture sheet according to claim 1, wherein the first protrusion is provided at the center of the first groove, and the second groove is provided at the center of the middle mold.
7. The mold device for making a multi-concave cell culture plate according to claim 1, wherein at least one protrusion is further disposed in the first groove, and the protrusion is connected to the groove wall of the first groove; the middle mould is also provided with at least one third groove matched with the bump.
8. The mold device for making a multi-concave cell culture sheet according to claim 1, wherein the smoothness of the metal mold is nano-scale.
9. The mold device for producing a multi-concave cell culture sheet according to claim 1, wherein the number of the concave surfaces and the second protrusions is not less than 100.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020224460.3U CN211807345U (en) | 2020-02-28 | 2020-02-28 | Die device for manufacturing multi-concave cell culture sheet |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020224460.3U CN211807345U (en) | 2020-02-28 | 2020-02-28 | Die device for manufacturing multi-concave cell culture sheet |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154649A (en) * | 2020-02-28 | 2020-05-15 | 苏州大学 | Seal for manufacturing multi-concave gel sheet and using method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154649A (en) * | 2020-02-28 | 2020-05-15 | 苏州大学 | Seal for manufacturing multi-concave gel sheet and using method |
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