CN212255862U

CN212255862U - Depth of field extended microscopic imaging three-dimensional reconstruction device

Info

Publication number: CN212255862U
Application number: CN202021223334.2U
Authority: CN
Inventors: 郭朝舜; 于佳; 欧阳峰
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2020-06-28
Filing date: 2020-06-28
Publication date: 2020-12-29
Anticipated expiration: 2030-06-28

Abstract

The utility model provides a depth of field extension microscopic imaging three-dimensional reconstruction device, a serial communication port, include: line structured light unit: the system is used for generating linear structured light and three-dimensionally reconstructing the surface appearance of a target object based on the linear structured light; a microscopic imaging unit: for microscopic imaging; a light splitting unit: the light source is used for introducing the line structure light generated by the line structure light unit into the microscopic imaging unit and dividing the line structure light into transmitted light and reflected light; electric objective table: the device is used for placing and moving a target object; mechanical fixing: used for fixing and mounting.

Description

Depth of field extended microscopic imaging three-dimensional reconstruction device

Technical Field

The utility model relates to a microscopic imaging field, in particular to depth of field extension microscopic imaging three-dimensional reconstruction device.

Background

In the field of microscopic research, such as material surface reconstruction, biomedicine, etc., a microscope is an indispensable powerful tool, which can help researchers to study the characteristics of objects from microscopic forms by observing and displaying the microstructure through physical magnification of an optical system, and especially in the centuries of birth of the microscope, every major breakthrough of biomedicine is related to the microscopic information.

With the development of computer technology, microelectronic technology and imaging technology, the development of optical microscopy technology enters into the acquisition and reconstruction of three-dimensional information from two-dimensional images, and the development of microscopic three-dimensional reconstruction technology as an important research branch of image analysis is rapid and the application value is continuously increased.

However, microscopic three-dimensional reconstruction still has certain problems, as shown below:

1. the existing microscopic three-dimensional reconstruction technology can not realize the real-time, high-efficiency and rapid measurement of a large number of samples under the high three-dimensional reconstruction precision;

2. in practical application, the existing microscopic three-dimensional reconstruction technology has the limitation of depth of field, and cannot realize high magnification and high resolution under large depth of field, so that the range of microscopic detection is influenced;

3. the existing microscopic three-dimensional reconstruction technology has higher requirements on a detection instrument and high cost.

Disclosure of Invention

The not enough of prior art more than, the utility model discloses to introduce microscopic formation of image based on the three-dimensional reconstruction technique of line structure light, realize in the microscope field that the surface topography to the little object is three-dimensional to be rebuild and the formation of image to add the digit and zoom and realize the extension of the depth of field of scanning formula thoroughly, and realize high accuracy, the three-dimensional reconstruction of real-time efficient through the adaptation of three-dimensional reconstruction algorithm and the method that the camera was markd, based on above, the utility model provides a three-dimensional reconstruction device of microscopic depth of field extension based on line structure light.

The utility model provides a depth of field extension microscopic imaging three-dimensional reconstruction device specifically includes

Line structured light unit: the system is used for generating linear structured light and three-dimensionally reconstructing the surface appearance of a target object based on the linear structured light;

a microscopic imaging unit: for microscopic imaging;

a light splitting unit: the light source is used for introducing the line structure light generated by the line structure light unit into the microscopic imaging unit and dividing the line structure light into transmitted light and reflected light;

electric objective table: the device is used for placing and moving a target object;

mechanical fixing: used for fixing and mounting.

Furthermore, the line structured light unit comprises a laser light source, a light beam expanding and collimating assembly, an adjustable line structured light generator and a reflector assembly which are coaxially connected in sequence.

Further, the microscopic imaging unit comprises a microscopic objective group, a digital zoom lens and an image acquisition device.

Further, the wavelength output by the laser light source is one of 380-780 nm.

Further, the beam expanding and collimating assembly comprises a laser beam expander and a collimator.

Further, the adjustable line structured light generator is an adjustable slit of 0-6mm for generating line structured light of different widths.

Further, the reflector assembly comprises a reflector and a fixing frame, and the light propagation direction of the line structure is changed to enter the microscopic imaging unit.

Furthermore, the microscopic imaging unit comprises a microscopic objective group, a digital zoom lens, a connecting pipe and an image acquisition device which are coaxially connected in sequence.

Further, the microscope objective group is a long-working-distance objective lens with the magnification of 1-20 times.

Furthermore, the digital zoom lens can continuously change the focal length under the driving of current, change the equivalent focal length of the microscopic imaging unit and realize real-time in-focus tomography.

Further, the light splitting unit comprises a light splitting prism and is arranged in the light path of the microscopic imaging unit.

The utility model has the advantages that:

1. the utility model combines the microscopic technology with the line structured light scanning three-dimensional reconstruction technology to realize the three-dimensional reconstruction of microscopic objects in the microscopic field;

2. by introducing the digital zoom lens, the in-focus scanning of the longitudinal fault is realized, the limitation of the depth of a microscope scene is solved, and the three-dimensional reconstruction of the surface appearance of a large-size target object with high magnification and high resolution is realized;

3. compare in the micro-three-dimensional reconstruction of tradition, confocal microscope for example, the utility model provides a system simple structure, rebuild fastly to the cost and the three-dimensional reconstruction cycle of instrument have been reduced.

Drawings

FIG. 1 is a schematic diagram of the system architecture.

Figure 2 a digital zoom lens tomography schematic.

Reference numerals:

1. the device comprises a laser light source 2, a laser beam expander 3, a collimator 4, an adjustable line structured light generator 5, a reflector assembly 6, a light splitting unit 7, a digital zoom lens 8, a microscope objective group 9, a connecting pipe 10, an image acquisition device 11 and an electric objective table.

Detailed Description

To facilitate understanding of the embodiments of the present invention, the following description will be made in terms of several specific embodiments with reference to the accompanying drawings, and each embodiment is not intended to limit the embodiments of the present invention.

It is to be noted that in the claims and the description of the present patent, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, the use of the verb "comprise a" to define an element does not exclude the presence of another, same element in a process, method, article, or apparatus that comprises the element.

Use the poroid as surveying the target object in this embodiment, as shown in fig. 1 the utility model discloses depth of field extension microscopic imaging three-dimensional reconstruction device includes:

line structured light unit, microscopic imaging unit, light splitting unit 6, electric objective table 11 and mechanical firmware.

The line-structured light unit comprises a laser light source 1, a laser beam expander 2, a collimator 3, an adjustable line-structured light generator 4 and a reflector component 5 which are coaxially connected in sequence; the laser beam emitted by the laser light source 1 sequentially passes through the laser beam expander 2 and the collimator 3 to obtain parallel light, the parallel light irradiates the adjustable line structure light generator 4 to generate line structure light, the line structure light with different widths is generated by adjusting the size of a slit of the adjustable line structure light generator 4, the size range of the slit is 0-6mm, the line structure light reaches the reflector group, and the reflector reflects the line structure light to enter the light splitting unit 6.

The light splitting unit 6 is coaxially and fixedly connected with the microscopic imaging unit and comprises a light splitting prism, the light splitting prism divides the entering line structure light into transmission light and reflection light, and the reflection light enters the microscopic imaging unit.

The microscopic imaging unit comprises a microscopic objective group 8, a digital zoom lens 7, a connecting pipe 9 and an image acquisition device 10 which are coaxially connected in sequence; the reflected light split by the light splitting unit 6 sequentially passes through the digital zoom lens 7 and the microscope objective group 8 to irradiate on the holed worms, the holed worms reflect the light back to the original light path, the reflected light reaches the light splitting unit 6 to be split into transmitted light and reflected light, and the transmitted light enters the image acquisition device 10 to obtain image information.

The digital zoom lens 7 can change the focal length so as to change the integral focal length of the microscopic imaging unit, as shown in fig. 2, the surface of the holed insect sample has fluctuant height, the depth of field of the microscopic objective lens group 8 is shallow, the holed insect sample is not focused at every position, the longitudinal focusing position of the microscopic objective lens group 8 is changed by changing the focal length of the digital zoom lens 7, and the focusing point of the microscopic objective lens group 8 shown in the figure can be O1-O-O2 so as to realize longitudinal tomographic scanning of the holed insect sample, thereby avoiding that the light beam can be focused on the holed insect even if the sample has fluctuant height.

The electric objective table 11 is provided with a hole worm sample, and the hole worm sample moves along the transverse position to realize transverse scanning of the hole worm.

Claims

1. A depth of field extended microscopy imaging three-dimensional reconstruction device is characterized by comprising:

a microscopic imaging unit: for microscopic imaging;

spectroscopic unit (6): the light source is used for introducing the line structure light generated by the line structure light unit into the microscopic imaging unit and dividing the line structure light into transmitted light and reflected light;

electric stage (11): the device is used for placing and moving a target object;

mechanical fixing: used for fixing and mounting;

the line-structured light unit comprises a laser light source (1), a laser beam expander (2), a collimator (3), an adjustable line-structured light generator (4) and a reflector component (5) which are coaxially connected in sequence; the microscopic imaging unit comprises a microscopic objective group (8), a digital zoom lens (7) and an image acquisition device (10).

2. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 1, characterized in that: the wavelength output by the laser light source (1) is one of 380-780 nm.

3. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 1, characterized in that: the adjustable line structured light generator (4) is a slit with adjustable width and is used for generating line structured light with different widths.

4. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 3, characterized in that: the width of the slit ranges from 0 mm to 6 mm.

5. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 1, characterized in that: the microscope objective group (8) is a long-working-distance objective lens, and the magnification is any magnification of 1-20 times.

6. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 1, characterized in that: the digital zoom lens (7) can continuously change the focal length under the driving of current, change the equivalent focal length of the microscopic imaging unit and realize real-time in-focus tomography.

7. The depth-of-field extended microscopy imaging three-dimensional reconstruction apparatus according to claim 1, characterized in that: the light splitting unit (6) comprises a light splitting prism and is arranged in the light path of the microscopic imaging unit.