CN212247073U - Bioreactor for cell and tissue culture - Google Patents
Bioreactor for cell and tissue culture Download PDFInfo
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- CN212247073U CN212247073U CN201922065905.8U CN201922065905U CN212247073U CN 212247073 U CN212247073 U CN 212247073U CN 201922065905 U CN201922065905 U CN 201922065905U CN 212247073 U CN212247073 U CN 212247073U
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Abstract
The utility model discloses a bioreactor for cell and tissue culture. Comprises a box body, a culture chamber, a gas conveying device and a control device. Wherein there is the key of opening the machine in the box outside, display screen and observation window, and the observation window can carry out direct observation to cell, the tissue in the culture chamber, need not to open the reactor repeatedly and observes, has avoided potential pollution hidden danger. The box is inside to have incandescent lamp, ultraviolet disinfection lamp, has at least one relatively independent cultivation unit in the cultivation chamber, and the pH value that every unit all can monitor the culture solution alone changes, and gas transmission device can control the air input of oxygen, carbon dioxide, and controlling means can temperature, oxygen concentration, carbon dioxide concentration in the real-time supervision reactor.
Description
Technical Field
The utility model relates to a bioreactor, in particular to a bioreactor for cell and tissue culture.
Background
With the continuous development of biotechnology, a plurality of devices for cell and tissue culture appear, but the existing cell and tissue culture devices are only used for targeted cell culture, the culture process and the change situation of the substances of the cells and the tissues in the culture process cannot be known, the process of cell culture cannot be accurately known, and the culture result can not be controlled, so that the existing cell culture devices can achieve the purpose of culture, but have low culture efficiency and poor effect.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a bioreactor for cell and tissue culture, which solves the problems in the prior art.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a bioreactor for cell, tissue culture, includes the box, cultivates cavity and gas delivery mechanism, cultivates in the cavity setting box, gas delivery mechanism sets up in box one side, gas delivery mechanism lets in oxygen and carbon dioxide respectively in the box at cultivateing the cavity place through different pipelines. Particularly, be equipped with two different gas transmission pipeline along the inner wall of box, all be equipped with a plurality of ventholes on these two gas transmission pipeline, gas delivery device can let in oxygen and carbon dioxide through these two gas transmission pipeline respectively and be used for cell culture in the space at culture chamber place in the box, and the volume of letting in of oxygen and carbon dioxide, adjust according to the numerical value that controlling means fed back, for example when oxygen is more, oxygen lets in speed can slow down, accelerate the speed of letting in of carbon dioxide, reach gaseous balance, gas delivery device's transport rate can be adjusted through valve manual, perhaps adjust through controlling means and automatically controlled valve.
Furthermore, at least two surfaces of the box body are provided with observation windows, such as the upper surface of the box body and the front surface of the box body, the observation windows are made of transparent materials, and the culture state of the culture chamber can be observed through the observation windows.
Further, at least two cultivation cavities have been placed to box inside, adopt different cultivation cavities, can carry out the same or different a plurality of cultivations under same environment, are favorable to improving cultivation efficiency, can also be used to contrast cultivation and observe.
Furthermore, at least one culture solution storage bottle and a culture plate are arranged in the culture chamber; at least two micro peristaltic pumps are arranged in the culture chamber, one micro peristaltic pump is used for pumping in, and the other micro peristaltic pump is used for pumping out, so that the culture medium flows.
Particularly, cultivate the chamber including the frame of cultivateing the chamber, cultivate the frame formation of chamber and can hold the cultivation chamber of cultivateing the board, the cultivation board is arranged in and cultivates the intracavity, cultivates the chamber and is a half open-ended container, is equipped with the baffle on the frame of cultivateing the chamber, and two miniature peristaltic pumps fixed mounting avoid taking place to contact with the culture solution of cultivateing the intracavity on the baffle. The culture solution storage bottle is arranged on one side of the culture chamber, and the culture chamber, the micro peristaltic pump and the culture solution storage bottle are connected into a culture solution circulation loop through the PE pipe.
Further, bioreactor still includes controlling means, controlling means is located the box next door of cultivateing the cavity place, is equipped with processor module and power module in the controlling means, and power module can be connected with processor module electricity, then for the treater power supply, also can further supply power for other power modules (for example display screen, start key, sensor etc.) in the box or directly through the treater, and power module itself can be the battery, perhaps, power module also can be with external power supply then internal power supply, the utility model discloses a processor module can adopt the most conventional treater model, can realize the input/output of signal and with power module connect into essential supply circuit can, the utility model discloses do not improve treater itself, can directly adopt the model on the market, the size can satisfy the requirement.
Further, be equipped with display screen and start key on the box, the display screen can be connected to the processor module among the controlling means to receiving processor module's information and showing, it is required to explain, the information that the display screen display treater sent is a conventional technique, the utility model discloses can adopt and realize this partial technical scheme among the prior art, the display screen can link to each other through processor module and power module and accept its power supply, also can directly link to each other with power module, accepts the power supply, and is same, no matter the display screen supplies power through which kind of mode, all is the conventional mode among the prior art, the utility model discloses do not improve this partial content, can directly adopt the scheme among the prior art to realize.
Furthermore, fluorescent lamps and ultraviolet lamps are installed on the inner side of the box body and can be used for irradiating the culture chamber, and the fluorescent lamps and the ultraviolet lamps can also be connected to a power module of the control device through a lead to take electricity.
Further, install the pH sensor on the culture solution storage bottle, the pH sensor stretches into in the culture solution storage bottle for test the pH value in the culture solution storage bottle in real time, thereby judge whether the culture solution in the culture solution storage bottle needs to be changed, the pH sensor can be pH test paper or pH electronic tester, if adopt pH electronic tester, then can be connected to controlling means through the wire, show pH value through the display screen, if be pH test paper, then can expose its one end in culture solution storage bottle for the observation.
Further, the culture plate in each of the culture chambers may be subjected to shake culture. Particularly, cultivate the chamber and have certain length or width, rock the box gently, the culture plate can be at the interior round trip movement of cultivation chamber, realizes rocking the cultivation.
Preferably, one end of the culture plate is provided with an adjusting plate of the culture plate, the adjusting plate is provided with an opening or a groove, the opening or the groove can be matched with a rocker driven by a motor, for example, one end of the rocker extends into the opening or the groove, when the rocker is driven by the motor to swing, the adjusting plate can drive the culture plate to swing back and forth around a rotating shaft in a small range, and swing culture is formed.
Further, the control device dynamically monitors the concentration of oxygen and carbon dioxide in the culture chamber. Specifically, the control device is provided with a gas sensing window on the inner wall of the box body where the culture chamber is located, the gas sensing window may be a window frame-shaped device having a mesh-shaped surface and allowing gas to pass through, gas detectors such as an oxygen detector and a carbon dioxide detector are provided in the window frame and used for detecting the concentration of oxygen and carbon dioxide in the space, the gas detectors are connected to the processor and transmit the detected values to the processor, and the processor may further display the detected gas concentration values through a display screen for easy observation.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses simple structure can keep the stability of box internal culture environment, and the experimenter can follow the temperature in the real-time observation cultivation cavity on the display screen, and oxygen concentration, carbon dioxide concentration and pH value have improved cell, tissue culture efficiency and experimental data accuracy nature, the utility model discloses can set up a plurality of cultivation cavities, carry out various cells, tissue culture experiments respectively, the culture state that simultaneously can be simple, convenient, quick detection cell, tissue.
Drawings
FIG. 1 is a schematic view of the overall external structure of the bioreactor of the present invention.
FIG. 2 is a schematic view of the internal structure of the bioreactor of the present invention.
FIG. 3 is a schematic view of the structure of a single culture unit.
FIG. 4 is a schematic view of the internal structure of the culture unit, showing the rocking structure of the culture plate.
Fig. 5 is a schematic view of fig. 4 from another direction.
In the figure: 1-box body, 1-1-observation window, 1-2-incandescent lamp, 1-3-ultraviolet lamp, 1-4-gas sensing window, 1-5-display screen, 1-6-start key, 2-culture unit, 2-1-culture solution storage bottle, 2-2-micro peristaltic pump, 2-3-pH sensor, 2-4-culture plate, 2-5-baffle, 2-6-PE tube, 2-7-adjusting plate, 2-8-rotating shaft, 2-9-hole or slot, 3-real-time internal environment monitoring module, 4-gas flow control module, 4-1-gas conveying pipeline, 5-motor and 6-rocker, 6-1-fixed terminal, 6-2 cam turntable, base 7.
Detailed Description
The technical solution in the embodiments of the present invention is clearly and completely described in the following embodiments with reference to the accompanying drawings.
It should be pointed out that the embodiment is for facilitating the technical solution of the present invention to be better understood by those skilled in the art, and should not be considered as a limitation of the present invention, and all other embodiments obtained by those skilled in the art without creative work belong to the scope of the present invention.
As shown in FIGS. 1-3, the utility model provides a bioreactor for cell and tissue culture, which comprises a box body 1, a culture chamber 2, a control device 3 and a gas delivery device 4 are arranged in the box body 1, an observation window 1-1 is arranged at the top and the left front side of the box body 1, the observation window is used for observing the condition in the box body 1, the control device 3 and the gas delivery device 4 are arranged at one side of the box body 1, a display screen 1-5 and a start key 1-6 are arranged on the outer surface of the side of the box body 1, correspondingly, a processor module and a power module are arranged in the control device 3, the processor module mainly plays a role of circuit connection, for example, a power supply circuit is established between some electric parts and the power module, and information collected by a sensor, such as temperature information and gas concentration information, is converted into information which can be displayed by the display screen, these are all the conventional functions of processor module, the utility model discloses can adopt the treater of ordinary commercial model to realize these functions, start key 1-6 and processor module connect and can be arranged in opening or close the power consumption part in processor module and the whole box, for example gas transmission device, display screen etc. as preferred, can also set up temperature sensor in the box 1, temperature sensor also can be connected to the processing module among controlling means 3 on for transmit the temperature information in the box 1. The display screens 1-5 are used for dynamically displaying the conditions of the temperature, the oxygen concentration, the carbon dioxide concentration and the pH value in the box body 1 and the culture chamber 2 in real time, and are convenient for a user to observe.
The box body 1 is also internally provided with an incandescent lamp 1-2 for lighting and increasing the temperature in the box body and an ultraviolet lamp 1-3 for disinfection and sterilization in the box body, and the incandescent lamp 1-2 and the ultraviolet lamp 1-3 can be powered by a power module in the control device 3.
In this embodiment, 4 culture chambers 2 are disposed in the housing 1, each culture chamber 2 has a culture solution storage bottle 2-1 and at least one culture plate 2-4, cells and tissues can be cultured on the culture plate 2-4, at least two micro peristaltic pumps 2-2 are disposed in the culture chamber, one is used for pumping in and one is used for pumping out, so that the culture medium flows, and generally, the flow rate of the culture medium is preferably set to 3-5 mL/min. The cell and tissue culture plate 2-4 can move in the culture chamber 2 to perform shaking culture for cells with different culture condition requirements.
As shown in figure 3, the culture chamber 2 comprises a frame of the culture chamber, the frame of the culture chamber forms a culture cavity capable of accommodating a culture plate, the culture plate 2-4 is arranged in the culture cavity, the culture cavity is a semi-open container, a baffle plate 2-5 is arranged on the frame of the culture cavity, two micro peristaltic pumps 2-2 are fixedly arranged on the baffle plate and are prevented from contacting with a culture solution in the culture cavity, therefore, even if the culture cavity shakes a little cell culture solution during shaking culture, the cell culture solution cannot splash onto the peristaltic pumps 2-2, a culture solution storage bottle 2-1 is arranged on one side of the culture cavity, and the culture cavity, the micro peristaltic pumps and the culture solution storage bottle are connected into a culture solution circulation loop through PE pipes 2-6. The cell sap is continuously circulated in the process of cell culture, so the cell sap in the culture solution storage bottles is also continuously replaced, each culture solution storage bottle is provided with a pH sensor 2-3, and the change of the pH value of the culture solution can be dynamically monitored.
As shown in FIGS. 4-5, a base 7 is provided in the cultivation unit 2, a rotation shaft 2-8 is provided on the base 7, the cultivation plate 2-4 can be installed on the rotation shaft 2-8, an adjustment plate 2-7 is provided on one side of the cultivation plate 2-4, an opening or a slot 2-9 is provided on the adjustment plate 2-7, a rocker 6 can be inserted into the opening or slot 2-8, the rocker 6 is made of a flexible material such as a spring rod or a rubber rod having a certain hardness but being bent and restored, one end of the rocker 6 is provided with a fixing terminal 6-1, such that the rocker 6 is coupled to the opening or slot 2-9 through the fixing terminal 6-1, the other end thereof is installed on a cam turntable 6-2, the cam turntable 6-2 can be driven to rotate by a motor 5, thereby driving the rocker 6 to rotate circularly, and the rocker 6 can further drive the culture plates 2-4 to swing at a small angle, so that the culture plates 2-4 can be used for shaking culture. Preferably, the motor 5 and the peristaltic pump 2-2 can both supply electricity through the power supply module in the real-time internal environment monitoring module 3, and preferably, one shaking culture device can be configured for each culture unit 2, and preferably, a part of the culture units 2 can also be selected for shaking culture setting.
Or, in other modes, the cam rotating disc 6-2 is not arranged, the rocker 6 can be driven by the motor 5 to rotate by selecting a cam rod, so that the height of the side of the adjusting plate 2-7 is changed, and the culture plate 2-4 is shaken.
In some preferred modes, a magnetic stirring device, such as a magnetic stirring rod, can be arranged on the culture plates 2-4 to perform clockwise or counterclockwise stirring culture.
The concentration of oxygen and carbon dioxide can be adjusted according to the culture requirements during the culture, for example, the temperature is controlled at 37 ℃, the concentration of oxygen is 95% and the concentration of carbon dioxide is 5% during the culture of high oxygen culture, the temperature is controlled at 37 ℃, the concentration of oxygen is 21% and the concentration of carbon dioxide is 5% during the culture of normal oxygen culture, and the adjustment can be carried out according to the actual requirements, mainly aiming at the proper environment of the cultured substance.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. A bioreactor for cell and tissue culture is characterized by comprising a box body (1), a culture chamber (2) and a gas conveying device (4), wherein the culture chamber (2) is arranged in the box body (1), the gas conveying device (4) is arranged on one side of the box body (1), and the gas conveying device (4) comprises at least two gas conveying pipelines (2-5) capable of being introduced into the box body (1) and used for introducing oxygen and carbon dioxide into a space where the culture chamber (2) is located in the box body (1);
at least one culture solution storage bottle (2-1) and a culture plate (2-4) are arranged in the culture chamber (2); at least two micro peristaltic pumps are arranged in the culture chamber (2) to enable the culture medium to flow.
2. The bioreactor for cell and tissue culture according to claim 1, wherein at least two surfaces of the box body (1) are provided with observation windows (1-1), the observation windows (1-1) are made of transparent materials, and the culture state of the culture chamber (2) can be observed through the observation windows (1-1).
3. Bioreactor for cell, tissue culture according to claim 1, characterized in that the inside of the tank (1) is equipped with fluorescent lamps (1-2) and ultraviolet lamps (1-3) that can be used to illuminate the culture chamber (2).
4. Bioreactor for cell, tissue culture according to claim 1, characterized in that at least two culture chambers (2) are placed inside the tank (1).
5. Bioreactor for cell and tissue culture according to claim 1, characterized in that the culture solution storage bottle (2-1) is equipped with a pH sensor (2-3).
6. Bioreactor for cell, tissue culture according to claim 1, characterized in that the culture plates (2-4) in each culture chamber (2) can be cultured with shaking.
7. The bioreactor for cell and tissue culture according to claim 1, further comprising a control device (3), wherein the control device (3) is arranged in the box body (1), the box body (1) is provided with a display screen (1-5) and a start key (1-6), and the display screen (1-5) and the start key (1-6) are connected with the control device (3).
8. Bioreactor for cell, tissue culture according to claim 7, characterized in that the control means (3) detect the oxygen and carbon dioxide concentrations inside the culture chamber (2) through gas sensing windows (1-4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922065905.8U CN212247073U (en) | 2019-11-26 | 2019-11-26 | Bioreactor for cell and tissue culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922065905.8U CN212247073U (en) | 2019-11-26 | 2019-11-26 | Bioreactor for cell and tissue culture |
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| CN212247073U true CN212247073U (en) | 2020-12-29 |
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| CN201922065905.8U Active CN212247073U (en) | 2019-11-26 | 2019-11-26 | Bioreactor for cell and tissue culture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940935A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
| CN117844629A (en) * | 2024-03-07 | 2024-04-09 | 柔脉医疗(深圳)有限公司 | Tissue culture liquid way detecting system |
-
2019
- 2019-11-26 CN CN201922065905.8U patent/CN212247073U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940935A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
| CN117844629A (en) * | 2024-03-07 | 2024-04-09 | 柔脉医疗(深圳)有限公司 | Tissue culture liquid way detecting system |
| CN117844629B (en) * | 2024-03-07 | 2024-05-28 | 柔脉医疗(深圳)有限公司 | Tissue culture liquid way detecting system |
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