CN212199250U

CN212199250U - Soil microorganism sample enrichment device

Info

Publication number: CN212199250U
Application number: CN202020688991.8U
Authority: CN
Inventors: 李杨; 池景良; 朱巍巍; 敖静; 高晓梅; 刘晓辉; 孙玉禄
Original assignee: LIAONING SCIENTIFIC ACADEMY OF MICROBIOLOGY
Current assignee: Yingkou Xingyuan Industrial Co.,Ltd.
Priority date: 2020-04-29
Filing date: 2020-04-29
Publication date: 2020-12-22
Anticipated expiration: 2030-04-29

Abstract

A soil microorganism sample enrichment device is provided with a coarse filtration column, a fine filtration column and a collection column, wherein the bottoms of the coarse filtration column, the fine filtration column and the collection column are respectively provided with a coarse filtration layer, a fine filtration membrane and a collection membrane, the lower part of the collection column is provided with a necking, and a lower cover is arranged on the necking; the upper end of the suspension mixing column is fixedly connected with a suspension mixing column, the upper end of the suspension mixing column is provided with an upper cover with an air hole, a rubber plug is arranged on the air hole, the middle of the upper cover is provided with an extension vent pipe extending downwards, the bottom of the extension vent pipe is provided with a vent disc, the edge of the vent disc is provided with a plurality of air outlets, the extension vent pipe is communicated with the air outlets of the vent disc, the upper opening of the extension vent pipe is connected with a gas cylinder, and the extension vent pipe is communicated with the gas cylinder. Simple structure, convenient to use, it is big to gather the sample volume once, can avoid silt to block up filter equipment, directly accomplish and filter work to obtain pollution-free, the microorganism sample that the enrichment degree is high simply high-efficiently.

Description

Soil microorganism sample enrichment device

Technical Field

The utility model relates to a soil microorganism sample analysis field, in particular to soil microorganism sample enrichment device.

Background

Soil microorganisms are the most important components in soil and comprise three major groups of bacteria, fungi, actinomycetes and the like. Soil microorganisms are also the most vital and active part of soil, are directly related to the effectiveness of soil nutrients, and play an important role in plant growth. The existing soil microorganism collection is mainly carried out by workers, the collected samples are few and difficult to enrich, and the microorganisms carried by the collected workers can pollute the samples and interfere with subsequent analysis.

CN209307369U discloses a "field sewage microbe filtering enrichment bag", which comprises a coarse filtering layer and a fine filtering layer which are separately arranged, wherein the bottoms of the coarse filtering layer and the fine filtering layer are respectively provided with a filter screen, and the mesh number of the filter screen positioned on the coarse filtering layer is 1/10 of the mesh number of the filter screen positioned on the fine filtering layer. Because the soil particulate matter has complex components and more impurities which are easy to block the filter screen, the field sewage microorganism filtering and enriching bag is difficult to apply to the soil microorganism enriching operation.

Disclosure of Invention

The to-be-solved technical problem of the utility model is to provide a soil microorganism sample enrichment device that simple high efficiency, microorganism enrichment degree are high, can effectively avoid sample pollution.

The utility model discloses a realize like this:

a soil microorganism sample enrichment device is provided with a coarse filtration column and a fine filtration column, wherein the bottoms of the coarse filtration column and the fine filtration column are respectively provided with a coarse filtration layer and a fine filtration membrane, and the soil microorganism sample enrichment device is characterized in that: a collecting column is fixedly connected below the fine filtering column, a collecting membrane is arranged in the collecting column, a necking is arranged at the lower part of the collecting column, and a lower cover is arranged on the necking; at the colating column upper end fixedly connected with and hang the post of mixing, be provided with the upper cover that has the gas pocket on hanging the post of mixing be provided with the plug on the gas pocket, be equipped with downwardly extending's extension breather pipe in the middle part of the upper cover, be provided with the aeration dish bottom the extension breather pipe, open at the aeration dish edge has a plurality of gas outlets, the extension breather pipe is linked together with the gas outlet of aeration dish, the extension breather pipe upper shed is connected with the gas cylinder, the extension breather pipe is linked together with the gas cylinder.

Further, the coarse filtration layer is provided with an upper filter cloth and a lower filter cloth which are arranged up and down, the middle parts of the upper filter cloth and the lower filter cloth are respectively provided with a pressure release valve so as to avoid the breakage of the upper filter cloth and the lower filter cloth caused by the blockage of the filter cloth by soil, the upper filter cloth is a non-woven fabric with the aperture of 500-800 microns, the lower filter cloth is a non-woven fabric with the aperture of 300-400 microns so as to realize the graded rough filtration of soil and reduce the pressure of the fine filtration, and support nets are arranged below the upper filter cloth and the lower filter cloth.

Further, the relief valve has hat form valve body, it has the round hole to open respectively on filter cloth, the lower filter cloth, and the round hole is passed from bottom to top to the valve body, and the valve body is followed the diameter and is greater than the round hole diameter is provided with the spring under the valve body between terminal surface and the support net up end to realize the pressure release.

Furthermore, the suspension column, the coarse filtration column, the fine filtration column and the collection column are sequentially connected through threads.

Furthermore, the fine filter membrane is made of a polypropylene microporous membrane with the aperture of 100-130 μm so as to realize fine filtration and ensure that impurities such as soil particles and the like are not left in the collecting column.

Furthermore, the collecting membrane is made of a polypropylene microporous membrane with the aperture of 0.22-10 μm, so that microorganisms can be enriched in the collecting column after waste liquid is filtered out.

Furthermore, the diameter of the necking is less than or equal to one third of the diameter of the upper end of the collecting column, so that the outflow speed of the filtered liquid is accelerated, drainage is realized, and the filtering efficiency is enhanced.

Furthermore, the volumes of the suspension column, the coarse filtration column, the fine filtration column and the collection column are sequentially smaller than 2/3 of the volumes of the suspension column, the coarse filtration column, the fine filtration column and the collection column, so that the filtration efficiency is ensured, and the sequential filtration layer by layer is realized.

The utility model has the advantages that:

simple structure and convenient use. Utilize the suspension to mix the post with soil sample suspension and mix the liquefaction, be convenient for microorganism enrichment operation on next step, once can gather a large amount of samples, and can avoid polluting the sample in the collection in-process and after gathering, sample suspension mixes the liquefaction after, guarantee microorganism enrichment through the ladder filtration, the dish of ventilating escape gas constantly keeps upper suspension misce bene simultaneously, and can increase and filter the pressure, avoid blockking up unable filtration because of silt, the work of filtering can continue to be accomplished under the condition that sets up messenger silt blockked up filter equipment of relief valve, thereby convenient, swiftly, obtain pollution-free simply high-efficiently, the microorganism sample that the enrichment degree is high.

Drawings

Fig. 1 is a schematic view of the present invention;

fig. 2 is a cross-sectional view of the present invention;

fig. 3 is a sectional view of the vent plate of the present invention taken along the line a-a.

In the figure: the device comprises a suspension mixing column 1, a rough filtration column 2, a rough filtration layer 201, an upper filter cloth 201A, a lower filter cloth 201B, a pressure release valve 202, a valve body 202A, a spring 202B, a support net 203, a fine filtration column 3, a fine filtration membrane 301, a collection column 4, a collection membrane 401, a reducing port 402, an upper cover 5, an air hole 501, a rubber plug 502, an extension vent pipe 503, a vent disc 504, a lower cover 6 and a gas cylinder 7.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

As shown in the figure, the soil microorganism sample enrichment device comprises a suspension column 1, a rough filtration column 2, a fine filtration column 3 and a collection column 4 which are sequentially connected through threads from top to bottom, wherein an upper cover 5 with an air hole 501 is arranged above the suspension column 1, and a rubber plug 502 is arranged on the air hole 501; a necking 402 is arranged below the collecting column 4, and a lower cover 6 is arranged on the necking 402; the volume of the coarse filter column 2 is less than 2/3 that of the suspension column 1, the volume of the fine filter column 3 is less than 2/3 that of the coarse filter column 2, the volume of the collection column 4 is less than 2/3 that of the fine filter column 3, and the diameter of the necking is less than or equal to 1/3 that of the upper end of the collection column; in this embodiment, the diameter of the suspension column 1 is 30mm, and the height thereof is 100 mm; the diameter of the coarse filter column 2 is 30mm, and the height thereof is 30 mm; the diameter of the fine filtering column 3 is 30mm, the lower part of the fine filtering column is conical, the diameter of the conical bottom is 15mm, the height of the conical is 10mm, and the total height of the fine filtering column is 20 mm; the diameter of the collecting column 4 is 15mm, and the height thereof is 10 mm; the height and diameter of the throat 402 are both 5 mm;

a coarse filter layer 201 is arranged at the bottom of the coarse filter column 2, a fine filter membrane 301 is arranged at the bottom of the fine filter column 3, and a collection membrane 401 is arranged at the bottom of the collection column 4;

the coarse filtration layer 201 comprises an upper filter cloth 201A and a lower filter cloth 201B which are arranged up and down, the upper filter cloth 201A is made of non-woven fabrics with the pore diameter of 520 mu m, the lower filter cloth 201B is made of non-woven fabrics with the pore diameter of 320 mu m, and a support net 203 is arranged below the upper filter cloth 201A and the lower filter cloth 201B;

the fine filter membrane 301 and the collecting membrane 401 are made of polypropylene microporous membranes, the aperture of the fine filter membrane 301 is 110 micrometers, and the aperture of the collecting membrane 401 is 0.22 micrometers;

the central positions of the upper filter cloth 201A and the lower filter cloth 201B are respectively provided with a pressure release valve 202, the pressure release valve 202 comprises a hat-shaped valve body 202A, a spring 202B is arranged below the valve body 202A, the upper filter cloth 201A and the lower filter cloth 201B are respectively provided with a round hole, the valve body 202A penetrates through the round holes from bottom to top, the lower edge of the valve body 202A is larger than the diameter of the round holes, and the lower part of the spring 202B is propped against a support net 203;

an extended vent pipe 503 extending downwards is arranged in the middle of the upper cover 5, a vent disc 504 is arranged at the bottom of the extended vent pipe 503, a plurality of air outlets are uniformly distributed on the edge of the vent disc 504, the extended vent pipe 503 is communicated with the air outlets of the vent disc 504, an air bottle 7 is connected to the upper opening of the extended vent pipe 503, and the extended vent pipe 503 is communicated with the air bottle 7.

When the device is used, a soil sample is extracted by using the suspension column 1, the rough filtration column 2 and the fine filtration column 3 are sequentially connected with the collection column 4, the lower cover 6 is covered, sterile water is injected into the suspension column 1, the upper cover 5 is covered, the device is shaken gently to enable the soil sample in the suspension column 1 to be fully mixed with the sterile water to form suspension, meanwhile, the air bottle 7 supplies air into the suspension column 1 through the extended vent pipe 503, the air is dissipated through the air outlet of the vent disc 504, the dissipated air stirs the suspension, the air hole 501 is sealed by the rubber plug 502, the lower cover 6 is opened, the pressure in the suspension column 1 is increased, the suspension is sequentially filtered through the rough filtration column 2 and the fine filtration column 3, at the moment, the filtered clear liquid is the target bacteria liquid, the target bacteria liquid is filtered through the collection column 4, microorganisms are left on the collection membrane 401, and the filtered waste liquid is discharged through the reduction port 402. When the upper filter cloth 201A is blocked, the pressure above the pressure release valve 202 is increased to a threshold value, the valve body 202A is pressed under the action of the pressure, the suspension liquid flows down through the round hole of the upper filter cloth 201A, when the lower filter cloth 201B is also blocked, the pressure release valve 202 on the valve body is opened after the pressure reaches the threshold value, the suspension liquid flows into the fine filter column 3, and after two times of pressure release, most of organic matters in the soil sample can be basically guaranteed to be enriched on the collection membrane 401.

While the embodiments of the invention have been described above, it is not intended to be limited to the details shown, or described, but rather to cover all modifications, which would come within the scope of the appended claims, and all changes which come within the meaning and range of equivalency of the art are therefore intended to be embraced therein.

Claims

1. The utility model provides a soil microorganism sample enrichment facility, has coarse filtration post and essence and strains the post, coarse filtration post, essence strain the post bottom and are equipped with respectively and filter coarse filtration layer, essence and strain the membrane, characterized by: a collecting column is fixedly connected below the fine filtering column, a collecting membrane is arranged in the collecting column, a necking is arranged at the lower part of the collecting column, and a lower cover is arranged on the necking; at the colating column upper end fixedly connected with and hang the post of mixing, be provided with the upper cover that has the gas pocket on hanging the post of mixing be provided with the plug on the gas pocket, be equipped with downwardly extending's extension breather pipe in the middle part of the upper cover, be provided with the aeration dish bottom the extension breather pipe, open at the aeration dish edge has a plurality of gas outlets, the extension breather pipe is linked together with the gas outlet of aeration dish, the extension breather pipe upper shed is connected with the gas cylinder, and the extension breather pipe is linked together with the gas cylinder.

2. The soil microorganism sample enrichment device of claim 1, wherein: the coarse filtration layer is provided with an upper filter cloth and a lower filter cloth which are arranged up and down, the middle parts of the upper filter cloth and the lower filter cloth are respectively provided with a pressure release valve, the upper filter cloth is a non-woven fabric with the pore diameter of 500-800 mu m, the lower filter cloth is a non-woven fabric with the pore diameter of 300-400 mu m, and support nets are arranged below the upper filter cloth and the lower filter cloth.

3. The soil microorganism sample enrichment device of claim 2, wherein: the pressure release valve is provided with a hat-shaped valve body, the upper filter cloth and the lower filter cloth are respectively provided with a round hole, the valve body penetrates through the round holes from bottom to top, the diameter of the lower edge of the valve body is larger than that of the round holes, and a spring is arranged between the lower end face of the valve body and the upper end face of the support net.

4. The soil microorganism sample enrichment device of claim 1, wherein: the suspension mixing column, the coarse filtration column, the fine filtration column and the collection column are sequentially connected through threads.

5. The soil microorganism sample enrichment device of claim 1, wherein: the fine filter membrane is made of a polypropylene microporous membrane with the aperture of 100-130 mu m.

6. The soil microorganism sample enrichment device of claim 1, wherein: the collecting membrane is made of a polypropylene microporous membrane with the aperture of 0.22-10 mu m.

7. The soil microorganism sample enrichment device of claim 1, wherein: the diameter of the necking is less than or equal to one third of the diameter of the upper end of the collecting column.

8. The soil microorganism sample enrichment device of claim 1, wherein: the volumes of the suspension column, the coarse filtration column, the fine filtration column and the collection column are sequentially less than 2/3 of the volumes of the suspension column, the coarse filtration column, the fine filtration column and the collection column.