CN212102807U

CN212102807U - Cell recovery device

Info

Publication number: CN212102807U
Application number: CN202020328274.4U
Authority: CN
Inventors: 张英慧; 高汉铭; 谭俊杰; 石素莹; 孙宁云; 吴俊迪
Original assignee: Foshan University
Current assignee: Foshan University
Priority date: 2020-03-16
Filing date: 2020-03-16
Publication date: 2020-12-08
Anticipated expiration: 2030-03-16

Abstract

The utility model discloses a cell resuscitating device, which comprises a base and an outer shell arranged above the base, wherein the top surface of the outer shell is provided with a first holding tank, an inner shell is arranged in the first holding tank, a heating device is arranged on the inner shell, the top surface of the inner shell is provided with a second holding tank, a transmission gear is arranged in the second holding tank, the bottom surface of the outer shell is provided with a first through hole, the bottom surface of the inner shell is provided with a second through hole, a driving motor is arranged on the base, the output end of the driving motor is connected with a transmission shaft, the transmission shaft is in transmission connection with the transmission gear, a plurality of main self-rotating frames are arranged in the second holding tank around the transmission gear, each main self-rotating frame comprises a main support shaft, a main gear and a main positioning frame, the main positioning frame is, the effect is better, is heated more evenly to overall structure is simple, and it is very convenient to use.

Description

Cell recovery device

Technical Field

The utility model relates to an experimental apparatus especially relates to a cell recovery device.

Background

Cell recovery, which is a process of re-culturing cells frozen in liquid nitrogen or a refrigerator at minus 80 ℃ after thawing, and recovering the growth of the cells, wherein when the cells are recovered to a normal temperature state, the morphological structure of the cells is kept normal, biochemical reaction can be recovered, and different from cell freezing, the temperature rise in the cell recovery process is fast, so that moisture is prevented from entering the cells in the thawing process, ice crystals are formed, and the survival of the cells is influenced, therefore, after the cells are taken out from a low temperature, the frozen stock solution is required to be completely thawed within 1 to 2 minutes, and if the recovery speed is too slow, the cells are damaged. Conventional cell is come back to life or consciousness and is carried the cell cryopreserved pipe with tweezers and put into the water bath and rock in the water bath from time to time, make it be heated evenly to water gets into the cell cryopreserved pipe and pollutes the cell when preventing the water bath, open the cell cryopreserved pipe before will cryopreserved pipe with the alcohol cotton ball and disinfect and dry, aseptic operation need all be noted to whole process, because the water bath probably causes the pollution of cell, thereby cause the loss of cell, and heating efficiency is lower, the cell cryopreserved pipe is inhomogeneous.

SUMMERY OF THE UTILITY MODEL

The present invention is directed to a cell resuscitator to solve one or more problems of the prior art, and to provide at least one useful choice or creation.

The utility model provides a solution of its technical problem is:

a cell resuscitation device comprises a base and an outer shell arranged above the base, wherein a first accommodating groove is formed in the top surface of the outer shell, an inner shell is arranged in the first accommodating groove, a heating device is arranged on the inner shell, a second accommodating groove is formed in the top surface of the inner shell, a transmission gear is arranged in the second accommodating groove, a first through hole is formed in the bottom surface of the outer shell, a second through hole is formed in the bottom surface of the inner shell, a driving motor is arranged on the base, the output end of the driving motor is connected with a transmission shaft, the rotation axis of the transmission shaft is arranged in the vertical direction, the transmission shaft sequentially penetrates through the first through hole and the second through hole and extends into the second accommodating groove, the transmission shaft is in transmission connection with the transmission gear, a plurality of main self-rotating frames are arranged in the, main rotation frame includes main tributary supporting axle, master gear, main locating rack, the bottom of main tributary supporting axle connect in on the diapire of second holding tank, the master gear pass through first bearing connect in on the main tributary supporting axle, the master gear with drive gear intermeshing, the axis of rotation of main tributary supporting axle sets up along vertical direction, main locating rack set up in on the master gear, the top surface of shell body is provided with sealed lid, sealed lid with the shell body is for dismantling the connection.

The technical scheme at least has the following beneficial effects: during the use, open the sealed lid on the shell body, the cell that will peripheral hardware freezes deposits the pipe and puts on main locating rack, close sealed lid again, start heating device and driving motor, heating device heats in to the second holding tank, driving motor's output passes through the transmission shaft and drives drive gear rotation, drive gear drives the master gear rotation rather than the meshing, thereby make cell on the main locating rack freeze deposits the pipe rotation, cell that the rotation state is down freezes deposits the pipe and is heated more evenly, no matter where the heating source cell freezes deposits the pipe equal thermally equivalent, thereby avoid cell to freeze and deposit a pipe one side cold, the hot problem of another side, the rate of heating is faster, the effect is better, it is more even to be heated, and overall structure is simple, it is very convenient to use.

As a further improvement of the above technical solution, the heating device includes a heating wire, and the heating wire is disposed around an outer side wall of the inner housing. The heating wire for heating is arranged outside the second accommodating groove, so that the arrangement of electrical elements in the second accommodating groove is reduced, and the electric heating wire is simpler.

As a further improvement of the technical scheme, a spiral groove is formed in the outer side wall of the inner shell, and the electric heating wire is arranged in the spiral groove. The heating wire is arranged in the spiral groove, so that the installation is more convenient, and heat generated by the heating wire is better conducted into the inner shell.

As a further improvement of the technical scheme, heat insulation cotton is arranged between the outer shell and the inner shell. Reducing the heat conduction from the inner shell to the outer shell.

As a further improvement of the technical scheme, a second bearing is connected between the transmission shaft and the first through hole, and a first sealing ring is arranged on the second bearing. The second bearing can make the connection between transmission shaft and the shell body tighter, utilize the loss of the reducible heat of first sealing washer.

As a further improvement of the above technical solution, a third bearing is connected between the transmission shaft and the second through hole, and a second seal ring is arranged on the third bearing. The third bearing can make the connection between transmission shaft and the interior casing tighter, utilize the loss of the reducible heat of second sealing washer.

As a further improvement of the above technical solution, a sub rotation frame is disposed in the second accommodating groove, the sub rotation frame includes a sub support shaft, a sub gear, and a sub positioning frame, a bottom end of the sub support shaft is connected to a bottom wall of the second accommodating groove, the sub gear is connected to the sub support shaft through a fourth bearing, the sub gear is engaged with the main gear, a rotation axis of the sub support shaft is disposed along a vertical direction, and the sub positioning frame is disposed on the sub gear. More sub self-rotating frames for placing the cell freezing tubes can be continuously expanded outwards in the second accommodating groove, the sub self-rotating frames are placed on the sub positioning frames, and the sub gears can also rotate on the sub supporting shafts by utilizing the meshing transmission of the sub gears and the main gear.

As a further improvement of the above technical solution, the main positioning frame is detachably connected to the main gear. The main positioning frame and the main gear can be conveniently disassembled and assembled, and the main positioning frames with different specifications can be selected according to different use requirements of the cell cryopreservation tube, so that the use is more flexible.

Drawings

In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is clear that the described figures represent only some embodiments of the invention, not all embodiments, and that a person skilled in the art can also derive other designs and figures from these figures without inventive effort.

Fig. 1 is a schematic view of the overall structure of the present invention.

In the drawings: 100-base, 110-transmission shaft, 200-outer shell, 210-heat-preservation cotton, 300-inner shell, 310-heating device, 320-transmission gear, 331-main supporting shaft, 332-main gear, 333-main positioning frame, 341-sub supporting shaft, 342-sub gear, 343-sub positioning frame and 400-sealing cover.

Detailed Description

The conception, the specific structure, and the technical effects produced by the present invention will be clearly and completely described below in conjunction with the embodiments and the accompanying drawings to fully understand the objects, the features, and the effects of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and other embodiments obtained by those skilled in the art without inventive labor based on the embodiments of the present invention all belong to the protection scope of the present invention. In addition, all the connection relations mentioned herein do not mean that the components are directly connected, but mean that a better connection structure can be formed by adding or reducing connection accessories according to the specific implementation situation. All technical characteristics in the invention can be interactively combined on the premise of not conflicting with each other.

Referring to fig. 1, a cell resuscitating device comprises a base 100 and an outer shell 200 disposed above the base 100, wherein a first accommodating groove is disposed on the top surface of the outer shell 200, an inner shell 300 is disposed in the first accommodating groove, a heating device 310 is disposed on the inner shell 300, a second accommodating groove is disposed on the top surface of the inner shell 300, a transmission gear 320 is disposed in the second accommodating groove, a first through hole is disposed on the bottom surface of the outer shell 200, a second through hole is disposed on the bottom surface of the inner shell 300, a driving motor is disposed on the base 100, an output end of the driving motor is connected with a transmission shaft 110, a rotation axis of the transmission shaft 110 is disposed along a vertical direction, the transmission shaft 110 sequentially penetrates through the first through hole and the second through hole and extends into the second accommodating groove, and the transmission shaft 110, encircle in the second holding tank drive gear 320 and be provided with a plurality of main from the revolving rack, main from the revolving rack includes main support shaft 331, main gear 332, main locating rack 333, the bottom of main support shaft 331 connect in on the diapire of second holding tank, main gear 332 connect through first bearing in on the main support shaft 331, main gear 332 with drive gear 320 intermeshing, the axis of rotation of main support shaft 331 sets up along vertical direction, main locating rack 333 set up in on the main gear 332, the top surface of shell body 200 is provided with sealed lid 400, sealed lid 400 with shell body 200 is for dismantling the connection.

By the above, during the use, open sealed lid 400 on shell body 200, the cell that will peripheral hardware freezes deposits the pipe and puts on main locating rack 333, close sealed lid 400 again, start heating device 310 and driving motor, heating device 310 heats in the second holding tank, driving motor's output passes through transmission shaft 110 and drives drive gear 320 and rotate, drive gear 320 drives main gear 332 rather than the meshing and rotates, thereby make the cell on main locating rack 333 freeze and deposit the pipe rotation, the cell that the rotation state was down freezes deposits the pipe and is heated more evenly, no matter where the heating source freezes deposits the pipe and all can the thermally equivalent, thereby avoid the cell to freeze and deposit a pipe one side cold, the problem that the other side is hot, the rate of heating is faster, the effect is better, it is heated more even, and overall structure is simple, and it is very convenient to use.

A wire hole for passing a power supply wire is formed in the outer casing 200, and the heating device 310 and an external power supply connecting wire can pass through the wire hole.

In some embodiments, the heating device 310 includes a heating wire, which is disposed around an outer sidewall of the inner housing 300. The heating wire for heating is arranged outside the second accommodating groove, so that the arrangement of electrical elements in the second accommodating groove is reduced, and the electric heating wire is simpler.

In some embodiments, the outer sidewall of the inner housing 300 is provided with a spiral groove in which the heating wire is disposed. The heating wire is disposed in the spiral groove, so that the installation is more convenient, and heat generated from the heating wire is better transferred into the inner case 300.

In some embodiments, the insulation cotton 210 is disposed between the outer shell 200 and the inner shell 300, and the outer shell 200, the insulation cotton 210, and the inner shell 300 are disposed side by side from outside to inside. The insulation wool 210 can reduce the heat conduction of the inner shell 300 to the outer shell 200.

When there is no bearing connection between the transmission shaft 110 and the first through hole, the outer housing 200 may be directly placed on the base 100, and in some embodiments, a second bearing is connected between the transmission shaft 110 and the first through hole, and a first sealing ring is disposed on the second bearing. The second bearing can make the connection between the transmission shaft 110 and the outer shell 200 more compact, and the first sealing ring can reduce the heat dissipation.

In some embodiments, a third bearing is connected between the transmission shaft 110 and the second through hole, and a second sealing ring is disposed on the third bearing. The third bearing can make the connection between the transmission shaft 110 and the inner housing 300 more tight, and the second sealing ring can reduce the heat dissipation.

In some embodiments, a sub rotation rack is disposed in the second receiving groove, the sub rotation rack includes a sub support shaft 341, a sub gear 342, and a sub positioning rack 343, a bottom end of the sub support shaft 341 is connected to a bottom wall of the second receiving groove, the sub gear 342 is connected to the sub support shaft 341 through a fourth bearing, the sub gear 342 is engaged with the main gear 332, a rotation axis of the sub support shaft 341 is disposed in a vertical direction, and the sub positioning rack 343 is disposed on the sub gear 342. More sub self-rotating racks for placing the cell freezing tubes can be further expanded outwards in the second accommodating groove, the sub self-rotating racks are placed on the sub positioning rack 343, and the sub gear 342 can also rotate on the sub supporting shaft 341 by utilizing the meshing transmission of the sub gear 342 and the main gear 332.

In some embodiments, the main positioning frame 333 is detachably connected to the main gear 332. The main positioning frame 333 and the main gear 332 can be conveniently disassembled and assembled, and the main positioning frame 333 with different specifications can be selected according to different use requirements of the cell freezing tube, so that the use is more flexible.

While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the invention is not limited to the details of the embodiments shown, but is capable of various modifications and substitutions without departing from the spirit of the invention.

Claims

1. A cell resuscitation device characterized by: comprises a base (100) and an outer shell (200) arranged above the base (100), wherein a first accommodating groove is formed in the top surface of the outer shell (200), an inner shell (300) is arranged in the first accommodating groove, a heating device (310) is arranged on the inner shell (300), a second accommodating groove is formed in the top surface of the inner shell (300), a transmission gear (320) is arranged in the second accommodating groove, a first through hole is formed in the bottom surface of the outer shell (200), a second through hole is formed in the bottom surface of the inner shell (300), a driving motor is arranged on the base (100), the output end of the driving motor is connected with a transmission shaft (110), the rotation axis of the transmission shaft (110) is arranged along the vertical direction, the transmission shaft (110) sequentially penetrates through the first through hole and the second through hole and extends into the second accommodating groove, and the transmission shaft (110) is in transmission connection with the transmission, encircle in the second holding tank drive gear (320) is provided with a plurality of main from revolving rack, main from revolving rack includes main support shaft (331), main gear (332), main locating rack (333), the bottom of main support shaft (331) connect in on the diapire of second holding tank, main gear (332) connect through first bearing in on main support shaft (331), main gear (332) with drive gear (320) intermeshing, the axis of rotation of main support shaft (331) sets up along vertical direction, main locating rack (333) set up in on main gear (332), the top surface of shell body (200) is provided with sealed lid (400), sealed lid (400) with shell body (200) are for can dismantling the connection.

2. A cell resuscitation device according to claim 1, wherein: the heating device (310) comprises a heating wire, and the heating wire is arranged on the outer side wall of the inner shell (300) in a surrounding mode.

3. A cell resuscitation arrangement according to claim 2, wherein: the outer side wall of the inner shell (300) is provided with a spiral groove, and the heating wire is arranged in the spiral groove.

4. A cell resuscitation device according to claim 1, wherein: and heat insulation cotton (210) is arranged between the outer shell (200) and the inner shell (300).

5. A cell resuscitation device according to claim 1, wherein: a second bearing is connected between the transmission shaft (110) and the first through hole, and a first sealing ring is arranged on the second bearing.

6. A cell resuscitation device according to claim 1, wherein: and a third bearing is connected between the transmission shaft (110) and the second through hole, and a second sealing ring is arranged on the third bearing.

7. A cell resuscitation device according to claim 1, wherein: the second accommodating groove is internally provided with a sub self-rotating frame which comprises a sub supporting shaft (341), a sub gear (342) and a sub positioning frame (343), the bottom end of the sub supporting shaft (341) is connected to the bottom wall of the second accommodating groove, the sub gear (342) is connected to the sub supporting shaft (341) through a fourth bearing, the sub gear (342) is meshed with the main gear (332), the rotation axis of the sub supporting shaft (341) is arranged along the vertical direction, and the sub positioning frame (343) is arranged on the sub gear (342).

8. A cell resuscitation device according to claim 1, wherein: the main positioning frame (333) is detachably connected with the main gear (332).