CN212077045U

CN212077045U - Salmonella detection pretreatment device

Info

Publication number: CN212077045U
Application number: CN202020220091.0U
Authority: CN
Inventors: 郭慧; 孙照辉; 孟东笙; 夏永浩; 杨跃; 燕傲蕾
Original assignee: Bozhou University
Current assignee: Bozhou University
Priority date: 2020-02-27
Filing date: 2020-02-27
Publication date: 2020-12-04
Anticipated expiration: 2030-02-27

Abstract

The utility model discloses a salmonella detects preprocessing device, the device comprises a device main body, first motor has been erect to the bottom of device main part, first motor rotates through the shaft coupling and is connected with the swivel mount, be provided with the centrifuge bowl on the swivel mount, the spline groove with swivel mount looks adaptation is seted up to the bottom of centrifuge bowl, the up end of centrifuge bowl articulates there is the closing cap, the right side of device main part is connected with the workstation, the drawing liquid device has been placed on the workstation. The salmonella detection pretreatment device of the utility model combines the operations of the solid sample and the liquid sample into one device in the pretreatment link, is convenient to use, realizes the dual-purpose of one device, integrates a plurality of pretreatment devices into the device, simplifies the steps, saves time and labor and improves the working efficiency; in the pretreatment link, the purity of the bacterial liquid is further improved through operations such as filtration, centrifugation and the like, and the method is suitable for different working conditions and brings better use prospect.

Description

Salmonella detection pretreatment device

Technical Field

The utility model relates to a microorganism safety monitoring field, in particular to salmonella detects preprocessing device.

Background

Food safety is a major problem related to human health and social development, and with the improvement of living standard of people, people pay more and more attention to the quality and safety of food, salmonella is one of common important food-borne pathogenic bacteria, is the most main and most amount of normal inhabitation bacteria in intestinal tracts of people and animals, does not cause diseases under normal conditions, but can cause infection if entering gall bladder, bladder and the like to generate peritonitis, cholecystitis, diarrhea and the like, so that the occurrence and diffusion of pathogens are effectively controlled, and an accurate and timely detection means is a key for preventing and controlling the spread of salmonella; the current salmonella needs to be pretreated before being detected, the obtained bacterium liquid with high purity can complete subsequent detection operation, the current industry lacks unified standard and simple means in the pretreatment link, and the solid sample and the liquid sample are treated separately, so that the operation steps are complicated, the working efficiency is low, and therefore, the salmonella detection pretreatment device is provided.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a main aim at provides a salmonella detects preprocessing device, can effectively solve the problem in the background art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a salmonella detection pretreatment device comprises a device main body, wherein a first motor is erected at the bottom of the device main body and is rotatably connected with a rotary seat through a coupling, a centrifugal cylinder is arranged on the rotary seat, a spline groove matched with the rotary seat is formed in the bottom of the centrifugal cylinder, a sealing cover is hinged to the upper end face of the centrifugal cylinder, a workbench is connected to the right side of the device main body, and a liquid pumping device is placed on the workbench;

the top of device main part sets up crushing case, the feed inlet has been seted up to the up end of crushing case, the inside intermediate position department of crushing case rotates and is provided with crushing wheel, the inside intermediate position department of crushing wheel is provided with the pivot, the inner wall a week of crushing case has welded a plurality of tooth sword of group, the fixed dissolution chamber that is provided with in below of crushing case, the left side of device main part is close the position of dissolution chamber and is provided with gets the cinder notch, the solenoid valve is installed to the bottom of dissolution chamber, the below of dissolution chamber is transversely erect the filter screen, the reservoir has been seted up to the inside of device main part and the below that is located the filter screen, the bottom intercommunication of reservoir has the catheter and the lower tip liquid outlet of catheter is located the centrifuge tube top.

Preferably, a limiting groove is formed in the device main body and used for placing the centrifugal cylinder.

Preferably, the liquid pumping device comprises a liquid pumping pump, a liquid pumping pipe set and a collecting cup.

Preferably, the bottom of the crushing box is provided with a discharge port, and the rotating shaft is rotatably connected with the second motor.

Preferably, a dissolving reagent is placed in the dissolving chamber.

Compared with the prior art, the utility model discloses following beneficial effect has: according to the salmonella detection pretreatment device, in the pretreatment link, the operations of a solid sample and a liquid sample are combined into one device, the use is convenient, the purpose of dual purposes of one device is realized, a plurality of pretreatment devices are integrated on the device, the steps are simplified, the time and the labor are saved, and the working efficiency is improved; in the pretreatment link, the purity of the bacterial liquid is further improved by means of filtration, centrifugation and the like, so that the subsequent detection is facilitated; the whole device is simple in design, convenient to operate, better in using effect compared with a traditional mode, and the parts which are not involved in the device are the same as or can be realized by adopting the prior art.

Drawings

FIG. 1 is a schematic view of the overall structure of a pretreatment device for Salmonella detection according to the present invention;

FIG. 2 is a view showing the connection between the centrifugal cylinder and the rotary base of the pretreatment device for Salmonella detection according to the present invention;

fig. 3 is a partial enlarged view of the salmonella detection pretreatment apparatus of the present invention shown in fig. 1.

In the figure: 1. a device main body; 2. a first motor; 3. a coupling; 4. rotating; 5. a centrifugal cylinder; 6. a spline groove; 7. sealing the cover; 8. a work table; 9. a liquid pumping device; 10. a crushing box; 11. a feed inlet; 12. a grinding wheel; 13. a rotating shaft; 14. a toothed cutter; 15. a dissolution chamber; 16. a slag taking port; 17. an electromagnetic valve; 18. a filter screen; 19. a liquid storage tank; 20. a catheter.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

As shown in fig. 1-3, a salmonella detection pretreatment device comprises a device main body 1, a first motor 2 is erected at the bottom of the device main body 1, the first motor 2 is rotatably connected with a rotary seat 4 through a coupling 3, a centrifugal cylinder 5 is arranged on the rotary seat 4, a spline groove 6 matched with the rotary seat 4 is formed in the bottom of the centrifugal cylinder 5 to play a role in connection and fixation, a sealing cover 7 is hinged to the upper end face of the centrifugal cylinder 5, a workbench 8 is connected to the right side of the device main body 1, and a liquid pumping device 9 is arranged on the workbench 8;

the top of the device main body 1 is provided with a crushing box 10, the upper end face of the crushing box 10 is provided with a feed inlet 11, the middle position inside the crushing box 10 is rotatably provided with a crushing wheel 12, the middle position inside the crushing wheel 12 is provided with a rotating shaft 13, a plurality of groups of serrated knives 14 are welded on one circle of the inner wall of the crushing box 10, a dissolving chamber 15 is fixedly arranged below the crushing box 10, a slag taking port 16 is arranged at the left side of the device main body 1 close to the dissolving chamber 15, an electromagnetic valve 17 is arranged at the bottom of the dissolving chamber 15, a filter screen 18 is transversely erected below the dissolving chamber 15, a liquid storage tank 19 is arranged inside the device main body 1 and below the filter screen 18, the bottom of the liquid storage tank 19 is communicated with a liquid guide pipe 20, and a liquid outlet at;

a limit groove is arranged in the device main body 1 for placing the centrifugal cylinder 5, so that the position of the centrifugal cylinder 5 is limited, and the centrifugal cylinder is prevented from moving; the liquid pumping device 9 comprises a liquid pumping pump, a liquid pumping pipe set and a collecting cup and is used for taking out supernatant liquid in the centrifuge tube 5; a discharge hole is formed in the bottom of the crushing box 10, and the rotating shaft 13 is rotatably connected with a second motor; the dissolution chamber 15 is filled with a dissolution reagent.

It should be noted that, the utility model relates to a salmonella detection pretreatment device, when in use, when being a liquid sample, the salmonella detection pretreatment device is directly put into a centrifuge tube 5, a sealing cover 7 is closed, a first motor 2 is started, a rotating seat 4 is driven to rotate at a high speed, so that the centrifuge tube 5 does a centrifugal motion, the liquid sample is centrifuged, standing and layering are rapidly completed, and a liquid pumping device 9 is utilized to pump supernatant liquid for detection; when the solid sample is prepared, the solid sample is firstly put into the crushing box from the feed port 11, the crushing wheel 12 is driven by the second motor to rotate at a high speed, the sample is cut and crushed by matching with a plurality of groups of toothed cutters 14, the generated powder falls into the dissolving chamber 15, a solvent is added into the dissolving chamber 15 to be dissolved for a period of time, the electromagnetic valve 17 is opened to discharge the solution, the residual residue is cleaned from the residue taking port 16, the solution passes through the filter screen 18, part of impurities are filtered, the rest is converged into the liquid storage tank 19, the solution is injected into the centrifugal cylinder 5 through the liquid guide pipe 20 at the bottom, and then the centrifugal liquid taking operation of the first part is repeated, so that the method is practical.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A salmonella detection pretreatment device comprising a device main body (1), characterized in that: a first motor (2) is erected at the bottom of the device main body (1), the first motor (2) is rotatably connected with a rotary seat (4) through a coupler (3), a centrifugal cylinder (5) is arranged on the rotary seat (4), a spline groove (6) matched with the rotary seat (4) is formed in the bottom of the centrifugal cylinder (5), a sealing cover (7) is hinged to the upper end face of the centrifugal cylinder (5), a workbench (8) is connected to the right side of the device main body (1), and a liquid pumping device (9) is placed on the workbench (8);

the top of device main part (1) sets up crushing case (10), feed inlet (11) have been seted up to the up end of crushing case (10), the inside intermediate position department of crushing case (10) rotates and is provided with crushing wheel (12), the inside intermediate position department of crushing wheel (12) is provided with pivot (13), the inner wall a week welding of crushing case (10) has a plurality of tooth sword (14) of group, the fixed dissolving chamber (15) that is provided with in below of crushing case (10), the position that the left side of device main part (1) is close to dissolving chamber (15) is provided with gets cinder notch (16), solenoid valve (17) are installed to the bottom of dissolving chamber (15), transversely erect filter screen (18) in the below of dissolving chamber (15), reservoir (19) have been seted up to the inside of device main part (1) and lie in the below of filter screen (18), the bottom intercommunication of reservoir (19) has catheter (20) and the lower tip of catheter (20) catheter The liquid outlet is positioned above the centrifugal cylinder (5).

2. The salmonella detection pretreatment apparatus according to claim 1, wherein: a limiting groove is formed in the device main body (1) and used for placing the centrifugal cylinder (5).

3. The salmonella detection pretreatment apparatus according to claim 1, wherein: the liquid drawing device (9) comprises a liquid drawing pump, a liquid drawing pipe set and a collecting cup.

4. The salmonella detection pretreatment apparatus according to claim 1, wherein: the bottom of the crushing box (10) is provided with a discharge hole, and the rotating shaft (13) is rotatably connected with the second motor.

5. The salmonella detection pretreatment apparatus according to claim 1, wherein: a dissolution reagent is placed in the dissolution chamber (15).