CN212051128U

CN212051128U - Pipe breaking device after drawing of Pasteur suction pipe

Info

Publication number: CN212051128U
Application number: CN202020307037.XU
Authority: CN
Inventors: 李利娜; 马超; 邵小光
Original assignee: Dalian Municipal Women And Children's Medical Center
Current assignee: Dalian Municipal Women And Children's Medical Center
Priority date: 2020-03-13
Filing date: 2020-03-13
Publication date: 2020-12-01
Anticipated expiration: 2030-03-13

Abstract

The invention discloses a tube breaking device for a drawn pasteur straw, which is applied to an auxiliary reproduction technology.A cavity is formed inside a hinged part, wherein one end of an upper clamp is hinged with one end of a lower clamp; the upper blade and the lower blade are positioned on the same plane, and the plane is parallel to the plane where the upper clamp and the lower clamp are positioned; the upper blade is connected to the upper clamp through an internal spring; the upper clamp is provided with a bolt hole, and the bottom end of a micrometer bolt penetrates through the bolt hole and is connected to one side of the upper blade facing the upper clamp; one side of the lower blade facing the lower clamp is fixed on the lower clamp; the opposite side edges of the upper blade and the lower blade are parallel to each other; the opposite side edges are perpendicular to the micrometer bolts; the cavity is also communicated with the outside through a suction pipe inserting channel vertical to the lower blade; the elongated column of the straw insertion channel is located between the upper blade and the lower blade. The invention greatly improves the cutting difficulty and accuracy of the pasteur pipette.

Description

Pipe breaking device after drawing of Pasteur suction pipe

Technical Field

The invention relates to an auxiliary reproduction technology, in particular to a post-drawing pipe breaking device for a pasteur straw.

Background

In the assisted reproduction technology, the transfer of the ovum and the embryo and the stripping of the ovum granular cells are realized, and two modes are generally adopted: 1. the conventional drawn Pasteur pipette is used after drawing the Pasteur pipette to a proper pore size and attaching a rubber cap. 2. The commercial glass/plastic pipe with the fixed aperture is connected with a special egg stripper and then can be used.

In most assisted reproduction laboratories, the transfer process of the ovum or embryo almost runs through the technology of the test tube infant laboratory, so the consumption of consumables is huge, and for economic reasons, most laboratories currently use the 1 st solution, i.e. manually drawn pasteur pipette, to transfer the ovum and embryo and to strip the ovum granulosa cells.

The diameter of the ovum and cleavage stage embryo is about 150um, and the diameter of the blastocyst is about 200 um. Therefore, the diameter of the pipette varies from stage to stage for ova and embryos. If the diameter of the suction pipe is too small, the ovum or embryo suction can be failed, or the ovum or embryo can be excessively squeezed in the suction process, so that the development potential of the subsequent embryo is influenced. If the straw aperture is too big, can greatly increased absorb the degree of difficulty, especially when the freezing point sample of vitrification, if the straw aperture is too big, can greatly increase the point sample degree of difficulty, the operator is difficult to accurate control point appearance volume, causes freezing effect to descend, influences the effect of unfreezing of ovum or embryo. Therefore, it is important for the operator to obtain a proper pipette hole diameter during the drawing of the Pasteur pipette.

At present, after an operator in an assisted reproduction laboratory draws a pasteur straw, the pasteur straw is observed by naked eyes under a microscope, and after the position of the pasteur straw is judged by experience, the pasteur straw is cut at a corresponding position by a grinding wheel or a glass needle so as to obtain the pasteur straw with a proper aperture. However, in actual operation, the pipe breaking process is often inaccurate, the aperture of the suction pipe is too large or too small, and the light person causes secondary rework of the operator, so that the working difficulty is greatly increased, and the working efficiency is influenced. The serious causes damage to the ovum or embryo, thereby affecting the potential for subsequent embryo development.

Disclosure of Invention

The invention aims to provide a post-drawing pipe cutting device for a pasteur straw, which can accurately cut the pasteur straw.

In order to achieve the purpose, the invention adopts the following technical scheme:

a post-drawing pipe breaking device for a pasteur suction pipe comprises an upper clamp and a lower clamp, wherein one end of the upper clamp is hinged with one end of the lower clamp, a cavity is formed inside the hinged part, and an upper blade and a lower blade are arranged in the cavity;

the upper blade and the lower blade are positioned on the same plane, and the plane is parallel to the plane where the upper clamp and the lower clamp are positioned;

one side of the upper blade facing the upper clamp is connected with the upper clamp through an internal spring; the upper clamp is provided with a bolt hole, and the bottom end of a micrometer bolt with the capability of measuring the self moving distance penetrates through the bolt hole and is connected to one side of the upper blade facing the upper clamp;

one side of the lower blade facing the lower clamp is fixed on the lower clamp; the opposite side edges of the upper blade and the lower blade are parallel to each other; the opposite side edges are perpendicular to the micrometer bolts;

the cavity is also communicated with the outside through a suction pipe inserting channel vertical to the lower blade; the elongated column of the straw insertion channel is located between the upper blade and the lower blade.

An external spring is connected between the upper clamp and the lower clamp.

The micrometer bolt penetrates through the fixed scale cylinder and the movable indicating cylinder, the movable indicating cylinder is fixed on the micrometer bolt, and the fixed scale cylinder is fixed on the upper clamp; scales are carved on the fixed scale cylinder, and the movable indication cylinder is sleeved outside the fixed scale cylinder.

Compared with the prior art, the method has the advantages that the distance between the blades can be accurately adjusted through the micrometer bolts, so that the Pasteur pipette with the accurate diameter is obtained, and compared with micro-cutting, the working strength is reduced on one hand, and the accuracy is greatly improved on the other hand.

Drawings

FIG. 1 is an external schematic view of the apparatus of the present invention;

FIG. 2 is a schematic view of the interior of the apparatus of the present invention.

In the figure, 1, upper clamp, 2, lower clamp, 3, external spring, 4, pipette insertion channel, 5, micrometer bolt, 6, upper blade, 7, lower blade, 8, internal spring.

Detailed Description

The following describes embodiments of the present invention with reference to the drawings.

As shown in fig. 1 to 2, the post-drawing pipe cutting device for the pasteur pipette of the invention comprises an upper clamp 1 and a lower clamp 2, wherein one end of the upper clamp 1 is hinged with one end of the lower clamp 2, a cavity is formed inside the hinged part, and an upper blade 6 and a lower blade 7 are arranged in the cavity;

the upper blade 6 and the lower blade 7 are positioned on the same plane, and the plane is parallel to the plane where the upper clamp 1 and the lower clamp 2 are positioned;

the side of the upper blade 6 facing the upper clamp 1 is connected to the upper clamp 1 through an internal spring 8; the upper clamp 1 is provided with a bolt hole, and the bottom end of a micrometer bolt 5 with the capability of measuring the self moving distance penetrates through the bolt hole and is connected to one side of the upper blade 6 facing the upper clamp 1;

one side of the lower blade 7 facing the lower clamp 2 is fixed on the lower clamp 2; the opposite side edges of the upper blade 6 and the lower blade 7 are parallel to each other; the opposite side is perpendicular to the micrometer bolt 5;

the cavity is also communicated with the outside through a straw inserting channel 4 which is vertical to the lower blade 7; the elongated column of the straw insertion channel 4 is located between the upper blade 6 and the lower blade 7.

An external spring 3 is also connected between the upper clamp 1 and the lower clamp 2.

The micrometer bolt 5 penetrates through the fixed scale cylinder and the movable indicating cylinder, the movable indicating cylinder is fixed on the micrometer bolt 5, and the fixed scale cylinder is fixed on the upper clamp 1; scales are carved on the fixed scale cylinder, and the movable indication cylinder is sleeved outside the fixed scale cylinder.

Before the micrometer screw bolt is used, the micrometer screw bolt 5 (which is equivalent to a screw bolt in a micrometer) is adjusted, so that the number displayed on the micrometer screw bolt 5 is the distance between the upper blade 6 and the lower blade 7.

When the glass straw cutting tool is used, the distance between the upper blade 6 and the lower blade 7 is changed through fine adjustment of the micrometer nut, and the distance is the diameter through which a glass straw can pass. The glass tube, which is an unbroken tube after drawing, is then inserted into the straw insertion passage 4 until the seizing is prevented from continuing to move forward. Then, the glass straw can be cut by the upper and lower blades through the extrusion of the external clamp, so that the straw with the ideal aperture can be obtained. The upper and lower blades may be diamond/quartz blades.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. The post-drawing pipe breaking device for the pasteur straw is characterized by comprising an upper clamp (1) and a lower clamp (2), wherein one end of the upper clamp (1) is hinged with one end of the lower clamp (2) to form a cavity in the hinged position, and an upper blade (6) and a lower blade (7) are arranged in the cavity;

the upper blade (6) and the lower blade (7) are positioned on the same plane, and the plane is parallel to the plane where the upper clamp (1) and the lower clamp (2) are located;

the side of the upper blade (6) facing the upper clamp (1) is connected to the upper clamp (1) through an internal spring (8); a bolt hole is formed in the upper clamp (1), and the bottom end of a micrometer bolt (5) capable of measuring the self moving distance penetrates through the bolt hole and is connected to one side, facing the upper clamp (1), of the upper blade (6);

one side of the lower blade (7) facing the lower clamp (2) is fixed on the lower clamp (2); the opposite side edges of the upper blade (6) and the lower blade (7) are parallel to each other; the opposite side edges are perpendicular to the micrometer bolts (5);

the cavity is also communicated with the outside through a straw inserting channel (4) vertical to the lower blade (7); the extension column of the straw insertion channel (4) is positioned between the upper blade (6) and the lower blade (7).

2. The post-drawing pipe-breaking device for the pasteur straws as recited in claim 1, wherein an external spring (3) is connected between the upper clamp (1) and the lower clamp (2).

3. The pasteur straw drawing post-pipe-breaking device according to claim 1, wherein the micrometer bolt (5) passes through a fixed scale cylinder and a movable indication cylinder, the movable indication cylinder is fixed on the micrometer bolt (5), and the fixed scale cylinder is fixed on the upper clamp (1); scales are carved on the fixed scale cylinder, and the movable indication cylinder is sleeved outside the fixed scale cylinder.