CN212016366U - Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease - Google Patents
Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease Download PDFInfo
- Publication number
- CN212016366U CN212016366U CN201922482648.8U CN201922482648U CN212016366U CN 212016366 U CN212016366 U CN 212016366U CN 201922482648 U CN201922482648 U CN 201922482648U CN 212016366 U CN212016366 U CN 212016366U
- Authority
- CN
- China
- Prior art keywords
- syringe
- heat
- stopper
- injector
- nipple
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A heat-insulating injector for the preparation for treating lumbar intervertebral disc degenerative disease comprises an injector and a limiting device, wherein the injector comprises an injector tube, a conical piston, a push handle, a nipple and an injection needle; the syringe tube is of a sealed double-layer structure, a heat-preservation interlayer is arranged between the double-layer structures, one end of the syringe tube is a nipple, an injection needle is welded in the nipple, a conical piston is arranged in the syringe tube, and the conical piston is outwards connected with a push handle; stop device includes threaded sleeve, stopper and internal thread pipe, threaded sleeve cup joints in the syringe nipple, has the screw thread on the threaded sleeve outer wall, internal thread pipe and stopper pass through inner wall screw thread and threaded sleeve connection, and the position of stopper for the syringe needle can be adjusted to the rotating stopper, and the internal thread pipe is used for fixed stopper. The wall of the heat-preservation injector is filled with heat-preservation materials, so that the temperature of contents is ensured, the activity of cells is favorably maintained, and the stress reaction of an organism is reduced.
Description
Technical Field
The utility model relates to the technical field of lumbar intervertebral disc medical treatment, in particular to a heat-preservation injector of a preparation for treating lumbar intervertebral disc degenerative diseases.
Background
Lumbago (LBP) is considered to be the leading cause of disability worldwide, and about 6.32 million worldwide is affected by lumbago. Although the direct cause of LBP is unknown, its symptoms are associated with degenerative deformation of the lumbar disc (IVD). The lumbar disc is a fibrocartilaginous tissue structure between two vertebrae. The lumbar intervertebral disc protrusion is one of the more common diseases, mainly because each part of the lumbar intervertebral disc (nucleus pulposus, annulus fibrosus and cartilage plates), especially the nucleus pulposus, has degenerative changes of different degrees, under the action of external force factors, the annulus fibrosus of the intervertebral disc is ruptured, and nucleus pulposus tissues protrude (or are separated) from the ruptured part to the back or the vertebral canal, so that adjacent spinal nerve roots are stimulated or pressed, and a series of clinical symptoms such as lumbar pain, numbness and pain of one lower limb or two lower limbs are generated. The incidence of lumbar intervertebral disc protrusion is highest in 4-5 parts of the waist and 5-1 parts of the waist, and accounts for about 95 percent.
Lumbar intervertebral disc protrusion is a common disease, and has seriously influenced the quality of life of people due to the characteristics of long-term treatment, no cure and repeated attack. Once a lot of people suffer from lumbar prominence, blind treatment is easy, which is a wrong way to do, and terminal regret can be caused by carelessness, so that the correct understanding of lumbar prominence and the adoption of the most appropriate treatment method are the key points for curing. The existing methods for treating lumbar disc herniation are many, but most of the existing methods can only relieve symptoms and cannot radically treat the lumbar disc herniation. However, the existing treatments suffer from respective drawbacks, as follows:
1. traction therapy is only applicable to patients with bulging intervertebral discs, and if the intervertebral discs are already protruded and prolapsed, traction therapy is completely ineffective and can aggravate the symptoms of the patients;
2. physical therapy includes methods such as electrotherapy, infrared irradiation, thermotherapy and the like, has basically the same therapeutic purposes as massage, acupuncture and moxibustion and the like, and is mainly used for relieving clinical symptoms of patients rather than radical treatment means;
3. the blocking treatment is divided into two application methods, one is intraspinal blocking, the other is nerve root blocking, the intraspinal blocking is to inject the medicament into the vertebral canal directly, the nerve root blocking is to inject the medicament around the nerve root, the blocking effect is to relieve pain, the oppression of the protrusion cannot be solved, and the pain symptom of the waist and the lower extremities can be recovered immediately after the medicament effect is blocked;
4. the needle knife therapy is a new treatment method developed on the basis of acupuncture and closed therapy, and aims to create conditions for enlarging the closed range, so that the small needle knife therapy is an improvement of the closed therapy, and the treatment effect is equivalent to the closed therapy;
5. conventional open surgery includes: total laminectomy, hemilaminectomy, trans-abdominal intervertebral disc surgery, vertebral body fusion, etc. The purpose of the operation is to directly cut the pathological lumbar disc nucleus pulposus and relieve the nerve root compression so as to achieve the treatment purpose. Due to the limitation of the special physiological position of the lumbar, the normal physiological structure of the lumbar vertebra is damaged by the operation, the operation damage is large, and a series of adverse reactions such as postoperative instability of the lumbar vertebra, postoperative scar tissue adhesion, accidental injury of nerve roots in the operation and the like are easily caused;
6. the intervertebral disc mirror minimally invasive surgery avoids the problem of large damage of the conventional open surgery, reduces the risk of the surgery and the occurrence of complications, reduces the damage to normal bone joints in the surgery process in the microsurgery and the arthroscope assisted lumbar disc surgery, but is difficult to cleanly and thoroughly remove the diseased lumbar disc nucleus pulposus after the surgery visual field becomes small, and increases the risk of unsuccessful surgery;
7. percutaneous puncture aspiration can obviously reduce the pressure in the intervertebral disc and the content of the herniated intervertebral disc, thereby relieving or eliminating the compression symptom of the herniation on the nerve. The method has the advantages that the injury is small in the operation process, but the defect is that the operation is mainly performed by decompression, the operation is effective to the bulging of the intervertebral disc, and the operation is forbidden in the types of prolapse, dissociation, combined calcification and the like of lumbar intervertebral disc, so that the operation surface is too narrow;
8. in recent years, ozone is adopted by a very few hospitals to treat lumbar protrusion diseases. Ozone injection can be theoretically used for gasifying and concentrating the nucleus pulposus of the lumbar vertebral disc, but the chemical reactions and complications of ozone entering the vertebral canal are still to be observed and studied further, and the success rate of the treatment method is not high from the current feedback of patients.
Stem cell transplantation therapy is an advanced medical technology, and brings hope for the treatment of some difficult and complicated diseases. Stem cell transplantation therapy is the transplantation of healthy stem cells into a patient's body to repair or replace damaged cells or tissues, thereby achieving the goal of healing. Stem cell transplantation has a wide range of therapies, and can generally treat neurological disorders, immune system disorders, and other surgical disorders. Stem cells, known in the medical community as "universal cells," can differentiate into a variety of functional cells or tissue organs. Stem cell transplantation therapy is the transplantation of healthy stem cells into a patient's body to repair or replace damaged cells or tissues, thereby achieving the goal of healing. According to the differentiation potential of the stem cells, the stem cells have application value in preparing the medicine for treating the degenerative disease of the lumbar intervertebral disc.
SUMMERY OF THE UTILITY MODEL
In view of the problems of the prior art, the present invention provides a preparation for treating degenerative diseases of lumbar intervertebral disc and a method for preparing the same, wherein the preparation comprises mesenchymal stem cells wrapped by hydrogel, the mesenchymal stem cells have self-renewal and multidirectional differentiation capabilities, and the differentiated cells and secreted growth-promoting factors thereof can effectively alleviate the occurrence and development of degenerative diseases.
A preparation for treating degenerative disease of lumbar intervertebral disc comprises mesenchymal stem cells encapsulated with hydrogel.
Further, the above preparation for treating degenerative diseases of lumbar intervertebral disc contains mesenchymal stem cells at a concentration of 1X 106one/mL.
The content of the utility model also provides a preparation method of the preparation for treating the degenerative disease of the lumbar intervertebral disc, which comprises the following steps:
firstly, extracting mesenchymal stem cells;
② the expression of surface antigens CD29, CD34, CD45, CD71, CD90 and CD106 is identified by immunofluorescence staining;
culturing mesenchymal stem cell to the third generation, digesting, culturing with conditioned medium of chondrocyte at a culture density of 1 × 105Per mL, 37 ℃, 5% CO2Culturing for 24 hours under the condition;
fourthly, hydrogel wraps the mesenchymal stem cells, and each 1mL of hydrogel contains 1 multiplied by 106Individual mesenchymal cells.
The utility model also provides a heat-preservation injector of the preparation for treating the degenerative disease of the lumbar intervertebral disc. According to the characteristic that living cells contained in the preparation need to be preserved, the heat preservation injector has the function of heat preservation. The utility model provides a heat preservation syringe technical scheme as follows:
a heat-insulating lumbar disc injector comprises an injector and a limiting device, wherein the injector comprises an injector tube, a conical piston, a push handle, a nipple and an injection needle; the syringe tube is of a sealed double-layer structure, a heat-preservation interlayer is arranged between the double-layer structures, one end of the syringe tube is a nipple, an injection needle is welded in the nipple, a conical piston is arranged in the syringe tube, and the conical piston is outwards connected with a push handle; stop device includes threaded sleeve, stopper and internal thread pipe, threaded sleeve cup joints in the syringe nipple, has the screw thread on the threaded sleeve outer wall, internal thread pipe and stopper pass through inner wall screw thread and threaded sleeve connection, and the position of stopper for the syringe needle can be adjusted to the rotating stopper, and the internal thread pipe is used for fixed stopper.
Further, the heat-insulating interlayer is filled with foam materials, heat-insulating cotton or heat-insulating rubber.
Furthermore, scales are marked on the outer wall of the injection needle, and the starting point of the scales is the needle point.
Furthermore, the outer walls of the internal thread pipe and the limiting stopper are in a insection shape.
The utility model has the advantages that:
the utility model discloses the preparation of treatment lumbar disc degeneration disease that the content provided contains mesenchymal stem cell, and existing research has shown that mesenchymal stem cell transplantation therapy can be used for treating lumbar disc degeneration disease, the utility model discloses a content is with injection preparation made of hydrogel parcel mesenchymal stem cell, and the hydrogel parcel is favorable to improving mesenchymal stem cell's survival rate, provides support for the growth and the reproduction of cell.
The utility model provides a heat preservation syringe is designed according to the characteristics that the preparation of treatment lumbar disc degeneration disease contains mesenchymal stem cell, and the pipe wall of syringe is bilayer structure, wherein fills insulation material, has the heat preservation effect, prevents that the injection liquid temperature change from causing the patient to be uncomfortable. The injector is provided with a positioning device to control the penetration depth during injection, so that the nerve is prevented from being damaged by too deep penetration, and the target point cannot be reached by too shallow penetration.
Drawings
FIG. 1 is a flow chart of the preparation of a preparation for treating degenerative diseases of lumbar intervertebral disc;
FIG. 2 is a schematic view of a heat-retaining syringe;
in the figure, 1-syringe barrel; 2-injection needle; 3-a threaded sleeve; 4-a stopper; 5-an internal threaded tube; 6-heat preservation interlayer; 7-a conical piston; 8-pushing the handle; 9-Nipple.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be combined to clearly and completely describe the technical solutions of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the scope of the present invention.
Example 1
This example is the preparation of a formulation for the treatment of degenerative diseases of lumbar intervertebral disc, comprising the following:
1 obtaining mesenchymal Stem cells
Bone marrow was extracted by puncture from the iliac spines of patients under sterile conditions, the resulting bone marrow was diluted with PBS buffer, nucleated cells were collected using Ficoll-Paque density gradient medium (1.077g/mL, GE Healthcare), centrifuged at 400 Xg for 35 minutes at room temperature, cell pellets were collected, washed twice with sterile PBS buffer, and finally resuspended in culture medium. The medium used was DMEM complete medium supplemented with 15% Fetal Bovine Serum (FBS), the medium was changed every other day, and nonadherent cells were removed.
1.1 identification of surface antigens of BMSCs
(1) Dripping the digested cells on a cover glass, soaking the cover glass overnight in an acid jar, washing the cover glass with tap water for 10 times, washing the cover glass with double distilled water for 3 times, and soaking the cover glass in 75% alcohol for later use;
(2) taking out the cover glass in 75% alcohol during coating, drying on alcohol lamp, and placing 35mm2In the petri dish, 0.5mL of polylysine with a concentration of 50. mu.g/mL was pipetted and dropped on a cover glass, and left at 37 ℃ for 1 hour, and excess polylysine was recovered. Irradiating with ultraviolet ray for 30min, and air drying in a clean bench;
(3) placing a cover glass which is sterilized in advance and coated with polylysine at 35mm2In Petri dishes, passage 5 BMSCs were digested with 0.25% trypsin at 1X 105Inoculation at a density of 5% CO at 37 ℃ per mL2Culturing under the condition;
(4) when the cells grow to 70% confluence, removing the culture medium, and performing immunofluorescenceThe expression of its surface antigens CD29, CD34, CD45, CD71, CD90, CD106 was identified by light staining. Washed with PBS, fixed with 4% paraformaldehyde, and left overnight at 4 ℃. After washing with PBS 3 times, the mixture was added dropwise with PBS + NaN3(0.02%) + BSA (3%) + TritonX-100 (0.2%) diluted monoclonal antibody were incubated for 90min at room temperature. PBS washing 3 times, nuclear with Hoechst33258 staining, room temperature for 5min and washing. The slide was mounted with 50% glycerol buffer and photographed by fluorescent microscope observation.
The normal mesenchymal stem cells have antigen expression of CD29, CD34, CD45, CD71, CD90 and CD106, and a positive result can be observed, and the nucleus can be observed to be blue after Hoechst33258 is stained.
It has been shown that chondrocyte culture media can stimulate differentiation of mesenchymal stem cells into chondrocytes.
2 obtaining chondrocyte culture Medium
Chondrocytes were cultured in DMEM complete medium containing 15% FBS until the cells were grown to a logarithmic growth phase, and conditioned medium in which chondrocytes were cultured was collected, filtered through a 0.22 μm filter, and 10% FBS was added thereto.
3 culturing mesenchymal stem cells
Culturing the mesenchymal stem cells obtained in the step 1 to the third generation, digesting, and culturing by using the chondrocyte conditioned medium obtained in the step 2, wherein the culture density is 1 multiplied by 105Per mL, 37 ℃, 5% CO2Incubated under conditions for 24 hours.
4 obtaining cell sap
Harvesting mesenchymal stem cells, washing with sterile normal saline for 3 times, and resuspending the obtained cells with sterile normal saline at a cell density of 1-9.6 × 107one/mL.
5-Encapsulated hydrogels
A0.8% hyaluronic acid hydrogel was prepared according to the HyStem Hydragel (BIOTIME) instructions of the hyaluronic acid hydrogel kit. 0.5mL of deionized water was added to the HyStem vial using a 1mL syringe, and the Extralik and HyStem were mixed at a ratio of 1: 4 to complete gelation within 20 min. When encapsulating cells, the reaction is carried out for 10min, then the solution is blown and sucked by a pipette to ensure that the cells are evenly distributed in the gel, and each 1mL of waterThe gel contains 1 × 106Individual mesenchymal cells.
Example 2
The preparation for treating degenerative disease of lumbar intervertebral disc prepared in example 1 contains stem cells and is in the form of gel, and the present invention also provides a syringe for injecting the obtained preparation.
A heat-insulating lumbar disc injector comprises an injector and a limiting device, wherein the injector comprises an injector tube 1, a conical piston 7, a push handle 8, a nipple 9 and an injection needle 2; the syringe tube 1 is a sealed double-layer structure, a heat-insulating interlayer 6 is arranged between the double-layer structure, one end of the syringe tube 1 is provided with a nipple 9, an injection needle 2 is welded in the nipple 9, a conical piston 7 is arranged in the syringe tube 1, and the conical piston 7 is outwards connected with a push handle 8; stop device includes threaded sleeve 3, stopper 4 and internal thread pipe 5, threaded sleeve 3 cup joints in syringe nipple 9, has the screw thread on the 3 outer walls of threaded sleeve, internal thread pipe 5 and stopper 4 are connected with threaded sleeve 3 through the inner wall screw thread, and stopper 4 can be adjusted for the position of syringe needle 2 to rotating stopper 4, and internal thread pipe 5 is used for fixed stopper 4.
Further, the heat-insulating interlayer 6 is filled with foam material, heat-insulating cotton or heat-insulating rubber.
Furthermore, scales are marked on the outer wall of the injection needle 2, and the starting point of the scales is the needle point.
Further, the outer walls of the internal thread tube 5 and the stopper 4 are in a insection shape.
The specific method of using the utility model is as follows:
according to the perspective development result, the position limiter 4 is adjusted to a proper position, the positioner 4 can be controlled to move along the axis direction of the injector by rotating the positioner 4, the positioner 4 is rotated to the proper position, and then the internal thread tube 5 is rotated, so that the internal thread tube 5 is tightly attached to the position limiter 4, and the purpose of fixing the position limiter 4 is achieved. Air in the injector is exhausted, the push handle 8 is pulled to suck the injection, the heat-insulating interlayer structure of the injector can play a heat-insulating role in the injection, namely the preparation for treating the lumbar disc degeneration disease prepared in the first embodiment, the lumbar disc injection is carried out according to the conventional injection method, and after the needle is inserted into a body, the injection needle 2 cannot go deep into the skin due to the blocking effect of the stopper 4, so that the injection success rate is improved, and the body is prevented from being damaged.
The details of the present invention are not described in detail, but are well known to those skilled in the art. Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and the technical solutions of the present invention are modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (4)
1. A heat preservation syringe of preparation for treating lumbar disc degeneration disease which characterized in that: the injection device comprises an injector and a limiting device, wherein the injector comprises an injector tube, a conical piston, a push handle, a nipple and an injection needle; the syringe tube is of a sealed double-layer structure, a heat-preservation interlayer is arranged between the double-layer structures, one end of the syringe tube is a nipple, an injection needle is welded in the nipple, a conical piston is arranged in the syringe tube, and the conical piston is outwards connected with a push handle; stop device includes threaded sleeve pipe, stopper and internal thread pipe, threaded sleeve pipe cup joints in the syringe nipple, has the screw thread on the threaded sleeve pipe outer wall, internal thread pipe and stopper pass through inner wall screw thread and threaded sleeve union coupling, and the position of stopper for the syringe needle can be adjusted to the rotating stopper, and the internal thread pipe is used for fixed stopper.
2. The insulated syringe of the preparation for treating degenerative disease of lumbar intervertebral disc according to claim 1, wherein: the heat-insulating interlayer is filled with foam materials, heat-insulating cotton or heat-insulating rubber.
3. The insulated syringe of the preparation for treating degenerative disease of lumbar intervertebral disc according to claim 1, wherein: the outer wall of the injection needle is marked with scales, and the starting point of the scales is a needle point.
4. The insulated syringe of the preparation for treating degenerative disease of lumbar intervertebral disc according to claim 1, wherein: the outer walls of the internal thread pipe and the limiting stopper are in a insection shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922482648.8U CN212016366U (en) | 2019-12-25 | 2019-12-25 | Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922482648.8U CN212016366U (en) | 2019-12-25 | 2019-12-25 | Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212016366U true CN212016366U (en) | 2020-11-27 |
Family
ID=73482763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922482648.8U Expired - Fee Related CN212016366U (en) | 2019-12-25 | 2019-12-25 | Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212016366U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067920A (en) * | 2019-12-25 | 2020-04-28 | 中国人民解放军联勤保障部队第九二〇医院 | Preparation for treating degenerative disease of lumbar intervertebral disc and preparation method thereof |
-
2019
- 2019-12-25 CN CN201922482648.8U patent/CN212016366U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067920A (en) * | 2019-12-25 | 2020-04-28 | 中国人民解放军联勤保障部队第九二〇医院 | Preparation for treating degenerative disease of lumbar intervertebral disc and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101279104B (en) | Preparation of collagen protein sponge containing growth factor | |
| CN212016366U (en) | Heat-preservation syringe of preparation for treating lumbar intervertebral disc degenerative disease | |
| CN108865986A (en) | For repairing articular cartilage damage/defect mescenchymal stem cell preparation and its preparation method and application | |
| CN102233144A (en) | Method for implementing subcutaneous tissue regeneration by using tissue engineering technology | |
| JP2022027930A (en) | Stem cell filtrate preparation and method for preparing the same | |
| CN109985064A (en) | Mescenchymal stem cell secretes the purposes of extract, mescenchymal stem cell secretion extract and preparation method thereof | |
| CN101999951B (en) | Memory alloy artificial cervical intervertebral disc | |
| CN204411031U (en) | Strut type fusion device | |
| CN111067920A (en) | Preparation for treating degenerative disease of lumbar intervertebral disc and preparation method thereof | |
| CN217948109U (en) | Platelet exosome enrichment tube | |
| CN104606667B (en) | BFGF bovine basic fibroblast growth factor gel | |
| US20230338430A1 (en) | Technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation | |
| CN113456807A (en) | Soft tissue filler and application thereof | |
| CN112870229A (en) | Mesenchymal stem cell preparation for treating knee osteoarthritis and research method thereof | |
| CN110742906A (en) | Application of mesenchymal stem cell paracrine factor in preparation of pain medicine | |
| CN107213455A (en) | A kind of stem cell medicine and its preparation method and application | |
| Sun et al. | Electroacupuncture-enhanced differentiation of bone marrow stromal cells into neuronal cells | |
| CN112972498A (en) | Method for extracting autologous hair follicle stem cell transplantation scar treatment | |
| CN108888633A (en) | A kind of gum mescenchymal stem cell preparation and its application and preparation method | |
| CN107029215A (en) | A kind of stem cell medicine and its preparation method and application | |
| CN107929726A (en) | A kind of stem cell compound injection and its application in anti-treating caput femoris necrosis medicine is prepared | |
| CN115245600A (en) | Biological membrane for repairing skin wound | |
| Kakouri et al. | Autologous fat grafting as a stand-alone procedure for post-mastectomy breast reconstruction and the potential for improvement using adipose-derived stem cells | |
| Sidhu et al. | Autologous fat transfer for orbital volume augmentation in sockets with small nonseeing eyes | |
| RU2405561C2 (en) | Method of treating thyroid gland diseases accompanied by hypothyroidism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201127 Termination date: 20211225 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |