CN112972498A - Method for extracting autologous hair follicle stem cell transplantation scar treatment - Google Patents
Method for extracting autologous hair follicle stem cell transplantation scar treatment Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0627—Hair cells
Abstract
The invention discloses a technical method for treating scars by hair follicle stem cell transplantation, which comprises the following steps: treatment of scar areas; extracting hair follicles and separating the hair follicles; culturing hair follicles in vitro; the special anesthetic is used and prepared, and the preparation and innovation of the scar anesthetic are changed, so that the effects of improving the blood supply of scars and softening the scars are achieved, and the growth of hair follicle stem cells is facilitated; transplantation of hair follicle stem cells. The invention extracts and separates out single hair follicle by the external culture technology of the hair follicle, and puts in special culture solution for culture, and the preparation and the use of special anesthetic achieve the purposes of improving the blood supply of scars, softening the scars, being beneficial to the growth of hair follicle stem cells, and finally realizing the purposes of inhibiting the hyperplasia of the scars, softening and repairing the scars and beautifying the scars.
Description
Technical Field
The invention relates to the technical field of hair follicle transplantation, in particular to a technical method for treating scars by hair follicle stem cell transplantation.
Background
The scar is the damage of physical, biological and chemical factors to human skinSoft tissueThe skin soft tissue is seriously damaged and can not be completely and normally repaired, and the fibrous tissue replaces and repairs the remained local symptoms which influence the appearance and the function. The scar brings great physical and mental pains to patients, especially scars left after burns, scalds and serious trauma.Scar hyperplasiaThe years of the period are almost overwhelming for the patient. The later atrophy period causes the patients to have complete facial disabilities and dysfunction, which causes great physical and cardiac double disorders.
Keloid scarThe surface of the swelling skin is tumor-like and hyperplastic, the surface is smooth, and the color is ruddy and brightOften with dilatationCapillary tube Blood vessel. Skin lesions extend outward from the edge and the crab legs deform. The skin injuries are different in size and shape, hard like cartilage, and have subjective symptoms of itching, pain and burning sensation. The pain is acute, and may be sensitive to nerve terminal conduction or the formation of microneuroma, even if the clothes and the like touch lightly, the pain is felt. The development is slow, most of the scar is enlarged continuously, and some scars have bright epidermis and peripheral tissues and are slightly white. There was little self-retraction and occasional malignant changes. Keloid scars are usually found on the chest, shoulders, neck, back and auricles, and are rarely found on the eyelids, palms, soles and external genitalia. Although keloid scars are relatively common, the cause of the keloid is unknown so far, which brings great difficulty to treatment. How to inhibitFiber cellThe wild proliferation of the liver, prevention of relapse and continuous growth become a difficult problem in the medical community at present. The following treatments are currently available: surgical excision, grinding,LaserRadiation, confinement, freezing, etc. Monotherapy often fails to achieve satisfactory results and tends to worsen the affected area. For example, the surgical resection has high recurrence rate, the laser and closure are often painful, and various diseases occur, irregular menstruation occurs in women, impotence and obesity occur in men, and the like. Dermabrasion, laser, freezing, etc. do not prevent recurrence, which is very troublesome for many patients and physicians. In a word, the traditional method has large wound, high treatment cost and easy formation of secondary scars, and partial patients still have unsatisfactory curative effect even if treated for multiple times. The hair follicle stem cell transplantation has the characteristics of minimally invasive, safety, durability and the like for treating scars, and has better curative effect for treating hypertrophic scars. The hair follicle stem cell can inhibitFiber cellProvides a more effective minimally invasive method for preventing the recurrence and the continuous growth of scars and treating the scars.
Disclosure of Invention
The invention aims to provide a technical method for treating scars by hair follicle stem cell transplantation. Calculating scar area and extracting hair follicle quantity by adopting a special method before operation; the individual hair follicles are extracted and separated by adopting an FUE technology, and are put into a special culture solution for culture, so that the survival rate of the hair follicles and the activity of hair follicle stem cells are improved, and the postoperative curative effect is ensured; through special preparation of a scar anesthetic and innovation of an anesthetic mode, the purposes of improving scar blood supply, softening scars and facilitating growth of hair follicle stem cells are achieved; the aims of inhibiting scar hyperplasia, softening and repairing scars, removing scars and beautifying scars are achieved through accurate planting, and the problems in the background art are solved.
In order to achieve the purpose, the invention provides the following technical scheme:
the technical method for treating scars by hair follicle stem cell transplantation comprises the following steps:
step S1, scar area calculation method and calculation of number of extracted hair follicles
Step S2, extracting hair follicles, namely extracting a follicular unit by adopting an FUE technology;
step S3, separating hair follicles, and separating individual hair follicles from the follicular units under an electron microscope;
step S4, culturing hair follicles in vitro, placing the extracted and separated individual hair follicles in a special culture solution for culturing for later use, and culturing to enhance the activity of hair follicle stem cells and promote the differentiation and proliferation of the hair follicle stem cells;
and step S5, the prescription and the anesthesia mode of the anesthetic are changed, and the preparation of the scar anesthetic and the innovation of the anesthesia mode are changed, so that the aims of improving the blood supply of scars, softening scars and facilitating the growth of hair follicle stem cells are achieved.
And step S6, transplanting the hair follicle, namely precisely planting the hair follicle in a specific area of the scar by adopting a special planting needle to promote the proliferation and differentiation of hair follicle stem cells and the formation of tissues, and achieving the purposes of inhibiting scar hyperplasia, softening and repairing the scar and removing and beautifying the scar.
Further, the specific method of step S1 is to draw the range of the scar with a marker pen, copy it on the preservative film and place it on the electrocardiographic paper for measurement, where each square grid of the electrocardiographic paper is 1 square centimeter, and calculate the number of covered squares, which is the total area of the scar. The number of follicular units to be extracted is calculated according to the area to be implanted, 5-50 follicular units per square centimeter.
Further, in step S2, the FUE technique is used to extract the hair follicle containing the hair follicle stem cells from the occipital hair or beard.
Further, in step S3, after the FUE technology is used to extract follicular units, individual follicles are separated under an electron microscope, and the follicles are separated under a state of 2-10 times amplification, so that the accuracy is up to 100%, and the follicles are prevented from being damaged during the separation process.
Further, in step S4, individual hair follicles are extracted and isolated by FUE technology, cultured in a special culture medium, and cultured at 0 to 4 ℃ for more than 60 minutes for future use.
Further, the main components of the special culture solution are 5% of glucose injection, compound glycyrrhizin injection and normal saline.
Furthermore, in step S5, the formula of anesthetic before scar anesthesia and the manner of anesthesia achieve the purposes of improving scar blood supply, softening scar, and facilitating the growth of hair follicle stem cells.
Further, in the step S5, the main components of the anesthetic are dexamethasone sodium phosphate injection, epinephrine injection, lidocaine injection, and normal saline; the scar anesthesia mode is local swelling anesthesia.
Further, in the step S6, the hair follicle planting is required to be accurate, the cultured individual hair follicles are placed into a hollow special planting needle, and then the hair follicle stem cells are directly pushed by the planting needle to form an angle of 5-80 degrees by the hair follicle as a support, so that the hair follicles are accurately planted in the preoperatively designed scar specific area. Depending on the site of implantation, 5-50 follicular units per square centimeter are achieved.
Further, the hair follicle unit contains 1-3 individual hair follicles.
Compared with the prior art, the invention has the following beneficial effects:
1. the calculation of scar area and the calculation of the number of extracted hair follicles, scars, especially hypertrophic scars and keloids, cannot be calculated in a normal manner due to the irregular bulges and edges. The method has the advantages that through a special proportional mode, the calculation is accurate, and the method is simple and feasible;
2. according to the invention, the individual hair follicles are extracted and separated by the hair follicle in-vitro culture technology and the FUE technology, the hair follicles containing hair follicle stem cells are extracted, so that the hair follicles are prevented from being damaged in the separation process, and the special culture solution is used for culturing to improve the survival rate and activity of the hair follicle stem cells;
3. the invention changes the preparation of the scar anesthetic and the anesthesia mode to improve the scar blood supply and soften the scar by innovating the prescription of the anesthetic and the anesthesia mode, and is beneficial to the growth of hair follicle stem cells;
4. the hair follicle stem cells obtained by extraction exist in the hair follicle bulge part between the sebaceous gland opening and the pileus erectus muscle attachment point. It has the characteristics of slow periodicity, strong self-renewal capacity, multi-differentiation potential, nondifferentiation, strong in vitro proliferation capacity and the like. The hair follicle stem cells belong to adult stem cells, are in a quiescent state in vivo, and show surprising proliferation capacity under the action of in vivo culture and internal environment. The hair follicle stem cells have high proliferation potential, grow clonally when cultured in vitro, can be induced to differentiate into cells such as neuron cells, glial cells, smooth muscle cells, melanocytes and the like, and can differentiate into neurons, melanocytes, keratinocytes and the like when implanted into a body. These cells are of great significance for scar repair. The research finds that the hair follicle stem cell has the multidirectional differentiation potential, can be differentiated into epidermis, hair follicle and sebaceous gland, and is involved in the process of healing skin wounds.
FIG. 1 illustrates the accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a flow chart of the technical method for treating scars by hair follicle stem cell transplantation of the invention.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, and it should be understood that the preferred embodiments described herein are merely for purposes of illustration and explanation and are not intended to limit the present invention.
The technical key points of the invention are as follows: 1. the scar area calculating method and the calculation of the number of extracted hair follicles are characterized in that a marking pen is used for drawing the range of scars, then the range of the scars is copied on a preservative film and is placed on an electrocardiograph paper for measurement, each square grid of the electrocardiograph paper is 1 square centimeter, and the number of covered squares is calculated to be the total area of the scars. The number of follicular units required for extraction was calculated according to the different sites, 5-50 follicular units per square centimeter needed to be implanted. 2. The in vitro culture method of hair follicle comprises extracting individual hair follicle by FUE technology, culturing in special culture medium containing 5% glucose injection, compound glycyrrhizin injection and normal saline at 0-4 deg.C for 60 min; 3. the formula and the anesthesia mode of the anesthetic, the main components of the anesthetic are dexamethasone sodium phosphate injection, lidocaine injection, epinephrine and normal saline; the scar anesthesia mode is local swelling anesthesia; by changing the preparation of the scar anesthetic and the innovation of the anesthetic mode, the scar blood supply is improved, the scar is softened, and the growth of hair follicle stem cells is facilitated.
The first embodiment is as follows:
step S1, scar area calculation method and calculation of number of extracted hair follicles
Step S2, extracting hair follicles, namely extracting a follicular unit by adopting an FUE technology;
step S3, separating hair follicles, and separating individual hair follicles from the follicular units under an electron microscope;
step S4, culturing hair follicles in vitro, placing the extracted and separated individual hair follicles into a special culture solution for later use, and culturing to enhance the activity of hair follicle stem cells and promote the differentiation and proliferation of the hair follicle stem cells;
step S5, the prescription and the anesthesia mode of the anesthetic are changed, and the preparation of the scar anesthetic and the innovation of the anesthesia mode are changed, so that the scar blood supply is improved, the scar is softened, and the growth of hair follicle stem cells is facilitated;
and step S6, transplanting the hair follicle, namely precisely planting the hair follicle in a specific area of the scar by adopting a special planting needle to promote the proliferation and differentiation of hair follicle stem cells and the formation of tissues, and achieving the purposes of inhibiting scar hyperplasia, softening and repairing the scar and removing and beautifying the scar.
In this embodiment, the specific method of step S1 is to draw the range of the scar with a marker pen, copy the range on the plastic wrap and place the film on the electrocardiograph paper for measurement, where each square is 1 square centimeter, and the number of covered squares is the total area of the scar. The number of follicular units required for extraction was calculated according to the different sites, 5-50 follicular units per square centimeter needed to be implanted.
In this embodiment, in step S2, the FUE technique is used to extract 1 unit of hair follicle containing hair follicle stem cells from the pillow hair or beard.
In this embodiment, in step S3, after the FUE technique is used to extract the hair follicle, the hair follicle is separated under an electron microscope, and the separation is performed under a state of 2-10 times magnification, so that the accuracy is up to 100%, and the hair follicle is prevented from being damaged during the separation process.
In this embodiment, in step S4, a single hair follicle is extracted and separated by using FUE technology, and the single hair follicle is cultured in a special culture solution, and cultured at 0-4 ℃ for more than 60 minutes for use, wherein the main components of the special culture solution are 5% glucose injection, compound glycyrrhizin injection and normal saline, and the survival rate of the hair follicle is improved by culturing the extracted and separated hair follicle in the special culture solution.
In this embodiment, in step S5, a scar anesthetic is innovated, and the scar anesthetic is composed of dexamethasone sodium phosphate injection, lidocaine injection, epinephrine injection, and physiological saline; the scar anesthesia mode is local swelling anesthesia.
In this embodiment, in step S6, the technical method for treating scars by hair follicle stem cell transplantation is characterized in that during planting, a special scar planting needle is used to implant hair follicle stem cells into a scar area at an angle of 5 ° to 80 ° depending on hair follicles.
In this embodiment, the follicular unit includes 1-3 individual follicles, which is convenient for calculation and is beneficial to accurately extracting the follicles.
Example two:
in this example, the effective rate of scar treatment by hair follicle melanocyte stem cell transplantation reaches more than 99%.
The technical difficulty and the operation steps of the invention are as follows:
the technical difficulty is as follows: the survival rate refers to whether the planted hair follicle stem cells can directly survive and is related to the later treatment effect, and the extracted autologous hair follicle stem cells have the characteristics of slow periodicity, strong self-renewal capacity, high proliferation capacity, multi-differentiation potential and the like, and can continuously repair scar tissues after transplantation.
The operation steps are as follows: 1. calculating the number of extracted hair follicles by an innovative scar area calculation method; extracting hair follicles containing hair follicle stem cells by an FUE technology, putting the hair follicles into a special culture solution for culture after extraction and separation, and culturing for 60 minutes at 0-4 ℃ for later use; the preparation of the scar anesthetic and the anesthesia mode are innovated; 2. the 'planting' is another key point related to the survival of the hair follicle stem cells, the planting depth can affect the low survival rate, the poor effect, different parts and scar types, and the difference of the planting quantity, the planting angle and the planting depth is large.
The principle of the invention is as follows: the invention is based on the characteristics of slow periodicity, strong self-renewal capacity, high proliferation capacity, multi-differentiation potential and the like of hair follicle stem cells. Depending on the environment, hair follicle stem cells can be differentiated into hair follicle cells, sebaceous gland cells, hair follicle epithelial cells, and the like. The cells have important significance for the reconstruction and regeneration of skin, so the cells can be successfully applied to the repair of scars and the treatment of various scars. Especially has obvious curative effect on hyperplastic scars and keloids.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The technical method for treating scars by hair follicle stem cell transplantation is characterized by comprising the following steps:
step S1, calculating a scar area and the number of extracted hair follicles;
step S2, extracting hair follicles, namely extracting a follicular unit by adopting an FUE technology;
step S3, separating hair follicles, and separating individual hair follicles from the follicular units under an electron microscope;
step S4, culturing hair follicles in vitro, placing the extracted and separated individual hair follicles in a special culture solution for later use, and culturing to enhance the activity of hair follicle stem cells and promote the differentiation and proliferation of the hair follicle stem cells;
step S5, the prescription and the anesthesia mode of the anesthetic are changed, and the preparation of the scar anesthetic and the innovation of the anesthesia mode are changed, so that the scar blood supply is improved, the scar is softened, and the growth of hair follicle stem cells is facilitated;
and step S6, transplanting the hair follicle, namely precisely planting the hair follicle in a specific area of the scar by adopting a special planting needle to promote the proliferation and differentiation of hair follicle stem cells and the formation of tissues, and achieving the purposes of inhibiting scar hyperplasia, softening and repairing the scar and removing and beautifying the scar.
2. The technical method for treating scars through hair follicle stem cell transplantation as claimed in claim 1, wherein a marking pen is used for drawing the range of scars, then the range of scars is copied on a preservative film and is placed on an electrocardiograph paper for measurement, each square grid of the electrocardiograph paper is 1 square centimeter, the number of covered squares is calculated to be the total area of the scars, 5-50 follicular units are implanted into each square centimeter according to different parts, and the number of extracted follicular units is calculated.
3. The specific method of step S2 is to extract 1 unit of hair follicle containing hair follicle stem cells from occipital hair or beard using FUE technology.
4. The technical method for treating scars through hair follicle stem cell transplantation as claimed in claim 2, wherein in the step S3, after the hair follicle is extracted by the FUE technology, the hair follicle is separated under an electron microscope, and the separation is carried out under a state of 2-10 times magnification, so that the accuracy rate is up to 100%, and the hair follicle is prevented from being damaged in the separation process.
5. The technical method for treating scars by hair follicle stem cell transplantation as claimed in claim 3, wherein in the step S4, individual hair follicles are extracted and separated by FUE technology, and cultured in a special culture solution for 60 minutes at 0-4 ℃ for standby.
6. The technical method for treating scars by hair follicle stem cell transplantation as claimed in claim 4, wherein the main components of the special culture solution are compound glycyrrhizin injection, physiological saline and 5% glucose injection.
7. The technical method for treating scars by hair follicle stem cell transplantation as claimed in claim 1, wherein the scar anesthetic and anesthetic mode in the step S5 are innovated, and the components of the scar anesthetic and anesthetic are dexamethasone sodium phosphate injection, epinephrine injection, lidocaine injection and normal saline; the scar anesthesia mode is local swelling anesthesia.
8. The technical method for treating scars through hair follicle stem cell transplantation as claimed in claim 6, wherein a special scar planting needle is used for planting the hair follicle stem cells into the scar area in an angle of 5-80 degrees based on hair follicles.
9. The technical method for treating scars through hair follicle stem cell transplantation as claimed in claim 7, wherein in the step S6, the planting of hair follicles is required to be accurate, the cultured individual hair follicles are placed into a hollow special planting needle, and then the hair follicles are directly pushed by the planting needle to be accurately planted in the preoperatively designed scar specific area, wherein the planting position is different, and 5-50 follicular units per square centimeter are achieved.
10. The technical method for treating scars by hair follicle stem cell transplantation according to any one of claims 1 to 8, characterized in that each follicular unit comprises 1 to 3 individual hair follicles.
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