CN212008621U - Novel fluorescence immunochromatography detection card for coronavirus - Google Patents
Novel fluorescence immunochromatography detection card for coronavirus Download PDFInfo
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- CN212008621U CN212008621U CN202020454231.0U CN202020454231U CN212008621U CN 212008621 U CN212008621 U CN 212008621U CN 202020454231 U CN202020454231 U CN 202020454231U CN 212008621 U CN212008621 U CN 212008621U
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Abstract
The utility model relates to a novel coronavirus's fluorescence immunochromatography detects card, include: the base is provided with a mounting groove; the cover body is made of shading materials and comprises a transparent observation window, a push-pull plate, a sample adding hole and a sample adding cover, and the sample adding cover is connected with the cover body; the push-pull plate is used for opening or shielding the observation window; the test strip is arranged in the mounting groove and observed below the observation window through the observation window, and comprises a plastic base plate, a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper. The utility model provides a pair of novel coronavirus's fluorescence immunochromatography detects card is located inclosed and under the environment of light-resistant with the test paper strip setting in the lid, instils into the serum through the application of sample hole when the test for the serum can be detected under inclosed environment, observes the testing result through the observation window, thereby can reduce aerosol transmission ability, reduces the infection risk of new coronavirus when detecting.
Description
Technical Field
The utility model belongs to the field of medical equipment, concretely relates to novel coronavirus's fluorescence immunochromatography detects card.
Background
The International Committee for the classification of Viruses (International Committee on Taxonomy of Viruses, ICTV) announces the formal name of the new type of coronavirus: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). On the day, the general work of World Health Organization (WHO) Tan dessel announced that pneumonia infected with the novel coronavirus would be formally named "COVID-19".
Transmission of viruses is caused by a number of factors, one of which, not negligible, is aerosol transmission. Although medical personnel have been adequately armed, cases of infection still occur. As a rapid detection means, the fluorescence immunochromatography is an important means for detecting the antibody or antigen of the novel coronavirus SARS-CoV-2 because the sensitivity of the fluorescence immunochromatography is far higher than that of a colloidal gold method. The fluorescence immunochromatographic detection card is mostly an open plate strip, a sample adding hole and a detection area of the fluorescence immunochromatographic detection card are directly exposed to air, aerosol is possibly formed along with the intensity of dripping, and the fluorescence immunochromatographic detection card is very dangerous to use in a laboratory without negative pressure and virus treatment. Therefore, it is very important to design a test strip card for detecting the new coronavirus SARS-CoV-2, which reduces the generation of aerosol as much as possible.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a novel coronavirus's fluorescence immunochromatography detects card to reduce the infection risk that novel coronavirus detected time measuring to produce through the aerosol.
A fluorescent immunochromatography detection card for novel coronavirus, which is used for detecting the novel coronavirus and comprises:
the base is provided with an installation groove, and the length direction of the installation groove extends along the length direction of the base;
the cover body is made of a shading material and comprises a transparent observation window, a push-pull plate, a sample adding hole and a sample adding cover, and the sample adding cover is connected to the cover body and is embedded into the sample adding hole to seal the sample adding hole; the push-pull plate is movably connected to the cover body and used for opening or shielding the observation window;
the test strip is arranged in the mounting groove and is arranged below the observation window so as to be observed through the observation window, the test strip comprises a plastic base plate, a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper, and the sample pad is arranged at one end of the plastic base plate; one end of the marker pad is in contact with the sample pad, and the other end is in contact with one end of the nitrocellulose membrane; the other end of the nitrocellulose membrane is in contact with the absorbent paper, wherein the marker pad comprises a carrier base layer, and a marker formed by lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres is sprayed on the carrier base layer; the nitrocellulose membrane comprises a detection line and a quality control line which are distributed along the length direction and are separated from each other, wherein the detection line is a new coronavirus recombinant antigen coated on the nitrocellulose membrane, and the quality control line is a rabbit anti-chicken lgY antibody coated on the nitrocellulose membrane.
Preferably, the base further comprises a plurality of clamping seats, and the clamping seats are arranged near the mounting groove and used for clamping the test strip.
Preferably, the base further comprises a guide part arranged at an end of the mounting groove and used for guiding the test strip to move into the mounting groove.
Preferably, the cover body further comprises a shutter corresponding to the mounting groove, the shutter is hinged to the cover body, and after the test paper strip rotates to enter the mounting groove, the shutter returns to the original position to seal the cover body.
Preferably, the area of the cross section of the sample application hole decreases in a direction away from the test strip.
Preferably, the cross-section of the well has a conical shape with an area decreasing in a direction away from the strip.
Preferably, the sample injection device further comprises a connecting belt, one end of the connecting belt is connected to the sample injection cover, and the other end of the connecting belt is connected to the cover body.
Preferably, the side of the plastic bottom plate is provided with a pressure-sensitive adhesive, and the sample pad, the marker pad, the nitrocellulose membrane and the absorbent paper are adhered to the plastic bottom plate through the pressure-sensitive adhesive.
Preferably, the sample pad is arranged at the end of the marker pad, the marker pad is arranged at the end of the nitrocellulose membrane, and the absorbent paper is arranged at the end of the nitrocellulose membrane.
Preferably, a handle is arranged at the end part of the push-pull plate, the cover body is provided with a groove body, and the push-pull plate is embedded into the groove body and shields the observation window; and the detection line and the quality control line of the nitrocellulose membrane of the test strip are positioned below the observation window.
Compared with the prior art, the fluorescence immunochromatographic detection card for the novel coronavirus, provided by the utility model, has the advantages that the test strip is arranged in the cover body and is in a closed and light-proof environment, the operation can be carried out in the light-proof environment so as to prevent the inactivation of the fluorescent group under visible light, and the error is reduced; when the test is carried out, the serum is dripped into the sample adding hole, so that the serum can be detected in a closed environment, and the detection result is observed through the observation window, thereby reducing the aerosol transmission capability and the infection risk of the new coronavirus during detection.
Drawings
FIG. 1 is a schematic diagram showing the structure of a fluorescence immunochromatographic detection card for a novel coronavirus according to the present invention.
Fig. 2 is a schematic sectional view at a-a in fig. 1.
FIG. 3 is a schematic diagram of the structure of the base and the test strip.
FIG. 4 is a schematic structural diagram of the test strip.
In the figure:
10-a cover body; 11-a viewing window; 12-a well; 13-a sample application cover; 131-a connecting band; 14-a shutter; 15-a push-pull plate; 151-a handle; 20-a base; 21-mounting grooves; 22-a card holder; 23-a guide; 30-a test strip; 31-a plastic base plate; 32-sample pad; 33-absorbent paper; 34-a marker pad; 35-nitrocellulose membrane; 351-detection line; 352-line of quality control.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
The fluorescent immunochromatographic detection card for the novel coronavirus is used for detecting the novel coronavirus. FIG. 1 is a schematic view showing the structure of a fluorescent immunochromatographic detection card for a novel coronavirus according to the present invention, and FIG. 2 is a schematic view showing the cross-sectional structure at A-A in FIG. 1. As shown in fig. 1 and fig. 2, the fluorescent immunochromatographic test card for detecting the novel coronavirus comprises a base 20, a cover 10 and a test strip 30.
The cover 10 covers the base 20 and is made of a light-shielding material. The cover body 10 comprises a transparent observation window 11, a push-pull plate 15, a sample adding hole 12 and a sample adding cover 13. The window 10 is made of a transparent material, and the push-pull plate 15 is movably attached to the upper surface of the cover and provided with a handle 151. In this embodiment, the cover 10 is provided with a groove, the push-pull plate 15 is embedded in the groove and shields the observation window, and the observation window can be opened to observe the detection result of the test strip when the push-pull plate 15 moves along the groove.
The sample adding cover 13 is connected to the cover 10 and is configured to be inserted into the sample adding hole 12 to close the sample adding hole 12. The cover body 10 further includes a door 14 corresponding to the mounting groove 21, the top of the door 14 is hinged to the cover body 10, and after the test paper strip 30 rotates the door 14 to enter the mounting groove 21, the door 14 returns to the original position to seal the cover body 10. The area of the cross section of the sample hole 12 decreases in a direction away from the test strip 30, and preferably, the area of the cross section of the sample hole 12 decreases in a conical shape away from the test strip 30. The sample addition lid 13 can be engaged with the sample addition well 12 to block the sample addition well 12. In this embodiment, the sample addition cover 13 is connected to the cover 10 by a connection band 131, one end of the connection band 131 is connected to the sample addition cover 13, and the other end is connected to the cover 10.
Fig. 3 is a schematic structural diagram of a base 20 and a test strip 30, and as shown in fig. 3, the base 20 is provided with a mounting groove 21, and a length direction of the mounting groove 21 extends along a length direction of the base 20. The base 20 further includes a plurality of clamping seats 22, and the clamping seats 22 are disposed near the mounting groove 21 and used for clamping the test strip 30. The base 20 further includes a guiding portion 23 disposed at an end of the mounting groove 21 for guiding the test strip 30 to move into the mounting groove 21. The test strip 30 is disposed in the mounting groove 21 and below the observation window 11.
FIG. 4 is a schematic structural diagram of the test strip. As shown in fig. 4, the test strip 30 is disposed in the mounting groove and includes a plastic base plate 31, a sample pad 32, a marker pad 34, a nitrocellulose membrane 35(NC membrane), and absorbent paper 33. The sample pad 32 is a sample pad 32 made of a glass fiber film or a non-woven fabric material. And the right end of the sample pad 32 is lapped over the left end of the marker pad 34, the right end of the marker pad 34 is lapped over the left end of the nitrocellulose membrane 35, and the left end of the absorbent paper 33 is lapped over the right end of the nitrocellulose membrane 35. The marker pad 34 comprises a carrier base layer, and the carrier base layer is coated with a marker formed by lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres. The nitrocellulose membrane 35 comprises a detection line 351 and a quality control line 352 which are distributed along the length direction and are separated from each other, wherein the detection line 351 is a new coronavirus recombinant antigen coated on the nitrocellulose membrane 35, and the quality control line 352 is a rabbit anti-chicken lgY antibody coated on the nitrocellulose membrane 35. Wherein, the detection line 351 and the quality control line 352 of the nitrocellulose membrane 35 of the test strip 30 are positioned below the observation window 11.
As the pathogen SARS-CoV-2 is a novel coronavirus, it has several structural proteins of spinous process protein S (spike), matrix protein M (matrix), envelope protein E (envelope) and nucleoprotein N (nuclear protein). Coronavirus S proteins are involved in binding of the virus to cellular receptors and in mediating fusion of the virus to host membranes. Meanwhile, the S protein is mainly responsible for inducing host immune response and virus neutralizing antibodies. The S protein also determines the tropism of the viral tissue. The S protein is called spike glycoprotein (spike glycoprotein) and is positioned at the outermost layer of the new coronavirus, like a protuberant 'crown'. The coronavirus nucleocapsid protein (N protein) has a structural role and is involved in RNA synthesis. The E protein has ion channel activity, and when the E protein plays a role of ion channel, a pentamer high-order structure is formed to play a role, coronavirus E and coronavirus M control the assembly of virus particles, and research proves that the E protein deletion mutant strain loses pathogenicity. Therefore, the test strip 30 adopts S protein or N protein expressed by genetic engineering to mix and coat, and respectively detects new coronavirus IgM and IgG in serum. Different test strips 30 are used for detecting the new coronavirus IgM and IgG respectively, so that interference in IgM and IgG detection can be avoided. After the serum is dropped through the sample adding hole 12, the detection result of the test strip 30 is observed through the observation window 11.
When the test paper strip is used, the test paper strip 30 is close to the shutter 14 and pushes the shutter 14 to rotate, and the test paper strip extends into the mounting groove 21 under the guidance of the guide part 23. After the test strip 30 completely enters the mounting groove 21, the shutter 14 is reset to the closed state by gravity, so that the test strip 30 is in the sealed state.
Then, the push-pull plate 15 is moved by the handle 151 to cover the observation window 11, so that the test strip 30 is in a sealed and light-proof environment.
Diluting a sample to be detected (taking serum as an example) and a sample diluent according to a ratio of 1:50, then opening the sample adding cover 13, adding 80ul of the diluted sample into the sample adding hole 11, covering the sample adding cover 13 after completing sample adding, and moving the sample from the sample pad 2 to the direction of the absorbent paper 5 under the action of the absorbent paper 5. Chromatography is carried out for 15min while avoiding strong light irradiation.
And finally, detecting the detection card by using a chromatography scanner through the observation window 11, acquiring fluorescence signals of a detection line 351 and a quality control line 352 by using the chromatography scanner, transmitting detection data to a client by using the chromatography scanner through Bluetooth, calculating a fluorescence signal intensity value of the detection line and a fluorescence signal intensity value of the quality control line by using the client according to the detection data, acquiring a standard curve of the coronavirus antibody of the detected dog from a cloud platform by using the client by scanning the bar code of the detection card, and calculating the content of the coronavirus antibody of the dog in the liquid to be detected according to the contrast relation between the standard curve and the fluorescence signal intensity values of the quality control line and the detection line by using the client.
According to the novel fluorescence immunochromatographic assay card for coronavirus, the test strip 30 is arranged in the cover body 10 and is in a sealed and light-proof environment, and the operation can be performed in the light-proof environment to prevent the inactivation of the fluorescent group under visible light, so that the error is reduced. When in test, the serum is dripped into the sample adding hole 12, so that the serum can be detected in a closed environment, and the detection result is observed through the observation window 11, thereby reducing the aerosol transmission capability and the infection risk of the new coronavirus during detection.
It is to be understood that the present invention is not limited to the above-described embodiments, and various changes and modifications may be made without departing from the spirit and scope of the present invention, and it is intended to cover such changes and modifications if they fall within the scope of the claims and their equivalents.
Claims (10)
1. A fluorescent immunochromatography detection card for novel coronavirus, which is used for detecting the novel coronavirus and is characterized by comprising the following components:
the base is provided with an installation groove, and the length direction of the installation groove extends along the length direction of the base;
the cover body is made of a shading material and comprises a transparent observation window, a push-pull plate, a sample adding hole and a sample adding cover, and the sample adding cover is connected to the cover body and is embedded into the sample adding hole to seal the sample adding hole; the push-pull plate is movably connected to the cover body and used for opening or shielding the observation window;
the test strip is arranged in the mounting groove and below the observation window so as to be observed through the observation window, the test strip comprises a plastic base plate, a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper, and the sample pad is arranged at one end of the plastic base plate; one end of the marker pad is in contact with the sample pad, and the other end is in contact with one end of the nitrocellulose membrane; the other end of the nitrocellulose membrane is in contact with the absorbent paper, wherein the marker pad comprises a carrier base layer, and a marker formed by lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres is sprayed on the carrier base layer; the nitrocellulose membrane comprises a detection line and a quality control line which are distributed along the length direction and are separated from each other, wherein the detection line is a new coronavirus recombinant antigen coated on the nitrocellulose membrane, and the quality control line is a rabbit anti-chicken lgY antibody coated on the nitrocellulose membrane.
2. The novel fluorescent immunochromatographic test card for coronaviruses according to claim 1, wherein the base further comprises a plurality of card holders, and the card holders are disposed near the mounting groove for holding the test paper.
3. The novel fluorescent immunochromatographic test card for coronaviruses according to claim 2, wherein the base further comprises a guide part provided at an end of the mounting groove for guiding the test strip to move into the mounting groove.
4. The card of claim 3, wherein the cover further comprises a door corresponding to the mounting slot, the door is hinged to the cover, and the door returns to the original position to seal the cover after the strip rotates into the mounting slot.
5. The novel fluorescent immunochromatographic test card for coronaviruses according to claim 4, wherein the area of the cross section of the well decreases in the direction away from the strip.
6. The novel fluorescent immunochromatographic test card for coronaviruses according to claim 5, wherein the cross-sectional area of the well has a conical shape which decreases in the direction away from the strip.
7. The novel fluorescent immunochromatographic assay card for coronaviruses according to claim 6, further comprising a connecting tape, one end of which is connected to the sample addition lid and the other end of which is connected to the lid body.
8. The novel fluorescent immunochromatographic detection card for coronaviruses according to claim 7, wherein the side of the plastic base plate is provided with a pressure-sensitive adhesive, and the sample pad, the marker pad, the nitrocellulose membrane and the absorbent paper are attached to the plastic base plate through the pressure-sensitive adhesive.
9. The novel fluorescent immunochromatographic detection card for coronaviruses according to claim 8, wherein the sample pad is attached to an end portion of the marker pad, the marker pad is attached to an end portion of the nitrocellulose membrane, and the absorbent paper is attached to an end portion of the nitrocellulose membrane.
10. The new type coronavirus immunochromatography test card as claimed in claim 9, wherein a handle is provided at an end of the push-pull plate, the lid body is provided with a groove body, and the push-pull plate is embedded in the groove body and shields the observation window; and the detection line and the quality control line of the nitrocellulose membrane of the test strip are positioned below the observation window.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020454231.0U CN212008621U (en) | 2020-03-31 | 2020-03-31 | Novel fluorescence immunochromatography detection card for coronavirus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020454231.0U CN212008621U (en) | 2020-03-31 | 2020-03-31 | Novel fluorescence immunochromatography detection card for coronavirus |
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| CN212008621U true CN212008621U (en) | 2020-11-24 |
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| CN202020454231.0U Active CN212008621U (en) | 2020-03-31 | 2020-03-31 | Novel fluorescence immunochromatography detection card for coronavirus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112540173A (en) * | 2020-12-02 | 2021-03-23 | 赛莱克斯生物科技(苏州)有限公司 | Sample adding device and auxiliary sample adding type test structure |
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2020
- 2020-03-31 CN CN202020454231.0U patent/CN212008621U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112540173A (en) * | 2020-12-02 | 2021-03-23 | 赛莱克斯生物科技(苏州)有限公司 | Sample adding device and auxiliary sample adding type test structure |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220614 Address after: 510000 room 602, No. 270, Kefeng Road, Huangpu District, Guangzhou City, Guangdong Province Patentee after: Guangzhou KangTeng Biotechnology Co.,Ltd. Address before: No.31 erheng Road, Dongpu Road, Guangzhou, Guangdong Patentee before: Cai Xiangsheng |
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| TR01 | Transfer of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A Novel Fluorescence Immunochromatographic Detection Card for Coronavirus Effective date of registration: 20220928 Granted publication date: 20201124 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: Guangzhou KangTeng Biotechnology Co.,Ltd. Registration number: Y2022980016414 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230922 Granted publication date: 20201124 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: Guangzhou KangTeng Biotechnology Co.,Ltd. Registration number: Y2022980016414 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A fluorescence immunochromatographic detection card for novel coronavirus Effective date of registration: 20230925 Granted publication date: 20201124 Pledgee: Bank of China Limited Guangzhou Development Zone Branch Pledgor: Guangzhou KangTeng Biotechnology Co.,Ltd. Registration number: Y2023980058735 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |