CN211976566U - LED infrared light device for in vitro cell culture - Google Patents
LED infrared light device for in vitro cell culture Download PDFInfo
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- CN211976566U CN211976566U CN202020640237.7U CN202020640237U CN211976566U CN 211976566 U CN211976566 U CN 211976566U CN 202020640237 U CN202020640237 U CN 202020640237U CN 211976566 U CN211976566 U CN 211976566U
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Abstract
The utility model provides a LED infrared light device for in vitro cell culture, wherein the LED lamp bead array is fixedly arranged on the lamp panel; the control circuit is respectively connected with the LED lamp beads and the power supply; the lamp plate sets up the tip of shell, control circuit set up in the inside of shell. The utility model provides a LED infrared light device for external cell culture, the infrared light of transmission can provide infrared irradiation to external animal cell culture systems such as culture medium uniformly. In addition, when animal cells are cultured in vitro, the LED infrared light device can improve the anti-apoptosis capacity of the cells, promote the rapid growth of the cells, improve the cell culture efficiency and solve the problem of low in vitro animal cell culture efficiency.
Description
Technical Field
The utility model belongs to the technical field of the lamps and lanterns technique and specifically relates to a LED infrared light device for external cell culture is related to.
Background
In vitro cell culture refers to a method for simulating in vivo environment (aseptic, proper temperature, pH value, certain nutrient element conditions, and the like) in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions. In vitro cell culture technology is an important and common technology in cell biology research methods, and a large number of cells can be obtained through cell culture, and the metabolism of the cells, the growth and the proliferation of the cells and the like can be researched.
In vitro cell (animal cell) culture often encounters the problems that the cell activity is not enough, some cells die during the culture process, and a sufficient cell number is not obtained. The existing method improves the culture efficiency by adjusting the components of the culture medium, but the effect is limited frequently.
Therefore, it is an urgent technical problem to provide an LED infrared light device for in vitro cell culture capable of forming infrared light with a specific wavelength and light intensity to improve the cell culture efficiency.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a LED infrared light device for external cell culture, the device can form the infrared light of specific wavelength and light intensity and improve the anti apoptosis ability of the animal cell of external cultivation, promotes the cell fast growth, improves cell culture efficiency, has overcome the problem that external animal cell culture is inefficiency.
The utility model provides a LED infrared light device for in vitro cell culture, which comprises a LED lamp bead array, a lamp panel, a control circuit, a power supply and a shell;
the LED lamp bead array is fixedly arranged on the lamp panel;
the control circuit is respectively connected with the LED lamp bead array and the power supply;
the lamp plate sets up the tip of shell, control circuit set up in the inside of shell.
In the utility model, the LED lamp bead array can emit infrared light; the lamp panel is used for supporting the LED lamp bead array, and the size of the lamp panel can be determined according to the size of the in-vitro cell culture system, so that the in-vitro cell culture system can be uniformly irradiated by infrared light; the control circuit and the power supply can control the LED lamp bead array to be switched on and off; the shell can protect inside control circuit to fix the lamp plate. When animal cell culture is carried out in vitro, the LED infrared light device is used for irradiating a cell culture system, so that cell growth can be promoted, cell apoptosis can be prevented, and cell culture efficiency can be improved.
Further, LED lamp pearl array includes the parallelly connected lamp pearl group of multiunit, and each lamp pearl group includes the lamp pearl of a plurality of series connection.
Set up multiunit lamp pearl group, can guarantee even external cell culture system that shines. The lamp bead groups are connected in parallel, and the control circuit of one lamp bead group is prevented from being broken down to influence the use of other lamp tube groups. The lamp beads of each lamp bead group are connected in series, so that the uniformity of opening and closing of each lamp bead group is guaranteed, and uniform irradiation of infrared light is guaranteed.
Further, the lamp bead group comprises a first lamp bead group and a second lamp bead group, the lamp panel is a circular lamp panel, and the first lamp bead group is annularly arranged around the circle center of the circular lamp panel;
the second lamp bead group surrounds the first lamp bead group and is arranged in a circular shape.
The first lamp bead group and the second lamp bead group are arranged in an annular shape. Can act as a light beam concentrator.
Further, the wavelength of the light wave formed by the LED lamp bead array is 760-980 nm.
The wavelength of the light wave is 760-980nm infrared light, which can promote cell metabolism and avoid apoptosis of cells caused by loss of metabolic function. At this wavelength, the cell growth is vigorous.
Further, the light wave intensity formed by the LED lamp bead array is 0.01J/cm2-80J/cm2。
The light wave wavelength is 760-doped 980nm and the light wave intensity is 0.01J/cm2-80J/cm2The efficiency of promoting the growth of the animal cells is stronger.
Further, the shape of the shell is funnel-shaped.
The funnel-shaped shell can reduce the occupied space of the LED infrared light device, and is convenient to install and use.
Further, the lamp plate is aluminium lamp plate.
The aluminum lamp panel is light in weight and does not rust.
The utility model provides a LED infrared light device for external cell culture, the infrared light of transmission can provide infrared irradiation to external animal cell culture systems such as culture medium uniformly. In addition, when animal cells are cultured in vitro, the LED infrared light device can improve the anti-apoptosis capacity of the cells, promote the rapid growth of the cells and improve the cell culture efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of an LED infrared light device for in vitro cell culture;
fig. 2 is a schematic diagram of an LED bead array.
Description of reference numerals:
1: an array of LED lamp beads; 2: a lamp panel; 3: a control circuit; 4: a power source; 5: a housing; 6: and (4) a lamp bead.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms also include the plural forms unless the context clearly dictates otherwise, and further, it is understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, devices, components, and/or combinations thereof.
The technical solution of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
The invention provides an LED infrared light device for in vitro cell culture, which comprises an LED lamp bead array 1, a lamp panel 2, a control circuit 3, a power supply 4 and a shell 5;
the LED lamp bead array 1 is fixedly arranged on the lamp panel 2;
the control circuit 3 is respectively connected with the LED lamp bead array 1 and the power supply 4;
The LED lamp bead array 1 comprises a plurality of groups of lamp bead groups connected in parallel, and each lamp bead group comprises a plurality of lamp beads 6 connected in series.
The lamp bead group comprises a first lamp bead group and a second lamp bead group, the lamp panel 2 is a circular lamp panel 2, the first lamp bead group is annularly arranged around the circle center of the circular lamp panel 2, and the second lamp bead group is circularly arranged around the first lamp bead group.
The wavelength of light waves formed by the LED lamp bead array 1 is 760 nm.
The light wave intensity formed by the LED lamp bead array 1 is 0.01J/cm2。
Example 2
The invention provides an LED infrared light device for in vitro cell culture, which comprises an LED lamp bead array 1, a lamp panel 2, a control circuit 3, a power supply 4 and a shell 5;
the LED lamp bead array 1 is fixedly arranged on the lamp panel 2;
the control circuit 3 is respectively connected with the LED lamp bead array 1 and the power supply 4;
The LED lamp bead array 1 comprises a plurality of groups of lamp bead groups connected in parallel, and each lamp bead group comprises a plurality of lamp beads 6 connected in series.
The lamp bead group comprises a first lamp bead group and a second lamp bead group, the lamp panel 2 is a circular lamp panel 2, the first lamp bead group is annularly arranged around the circle center of the circular lamp panel 2, and the second lamp bead group is circularly arranged around the first lamp bead group.
The wavelength of light waves formed by the LED lamp bead array 1 is 870 nm.
The light wave intensity formed by the LED lamp bead array 1 is 40J/cm2。
The housing 5 is funnel shaped.
The lamp panel 2 is an aluminum lamp panel.
Example 3
The invention provides an LED infrared light device for in vitro cell culture, which comprises an LED lamp bead array 1, a lamp panel 2, a control circuit 3, a power supply 4 and a shell 5;
the LED lamp bead array 1 is fixedly arranged on the lamp panel 2;
the control circuit 3 is respectively connected with the LED lamp bead array 1 and the power supply 4;
In order to improve the illumination intensity, the LED lamp bead array 1 comprises a plurality of groups of lamp bead groups connected in parallel, and each lamp bead group comprises a plurality of lamp beads 6 connected in series.
On the basis of the device, lamp pearl group includes first lamp pearl and second lamp pearl group, and lamp plate 2 is circular lamp plate 2, and first lamp pearl group is cyclic annular arrangement around the centre of a circle of circular lamp plate 2, and second lamp pearl group is the circle around first lamp pearl group and arranges to this realization is to the even shining of external cell culture system.
In order to further improve the in vitro cell culture efficiency, the wavelength of light waves formed by the LED lamp bead array 1 is 980 nm.
Furthermore, in order to promote the growth of in vitro cells, the light wave intensity formed by the LED lamp bead array 1 is 80J/cm2。
On the basis of the above scheme, in order to realize the simplicity of the device in use and installation, the shape of the shell 5 is funnel-shaped.
The lamp plate 2 is an aluminum lamp plate, and can prevent the device from rusting and reduce the weight of the device.
To further illustrate that the LED infrared light device for in vitro cell culture provided by the present invention has the effects of improving the anti-apoptosis ability of cells, promoting rapid growth of cells, and improving the cell culture efficiency, the inventors have developed a conventional culture experiment of Adipose-derived stem cells (ADSCs).
Experiment one: with the LED infrared light device provided in example 1, the emission wavelength was 760nm, and the light intensity was 0.01J/cm2The infrared light irradiates an adipose-derived stem cell culture system, and SRB protein detection is carried out after 24 hours (SRB is a protein binding dye, is combined with basic amino acid in biological macromolecules, and the color change of the SRB is in direct proportion to the living cell protein), so that the number of the SRB protein of the living cells is obviously higher than that of a control group, and the increase amplitude is 26%.
Experiment two: using the LED infrared light device provided in example 2, the emission wavelength was 870nm, and the light intensity was 40J/cm2The number of the SRB protein of the survival cells is obviously higher than that of a control group by using an infrared light device for irradiation and detecting the SRB protein after 24 hours, and the increase amplitude is 40 percent.
Experiment three: using the LED Infrared light device provided in example 3, the wavelength for emission was 980nm, and the light intensity was 80J/cm2The number of the SRB protein of the survival cells is obviously higher than that of a control group by using an infrared light device for irradiation and detecting the SRB protein after 24 hours, and the increase amplitude is 34 percent.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.
Claims (7)
1. An LED infrared light device for in-vitro cell culture is characterized by comprising an LED lamp bead array (1), a lamp panel (2), a control circuit (3), a power supply (4) and a shell (5);
the LED lamp bead array (1) is fixedly arranged on the lamp panel (2);
the control circuit (3) is respectively connected with the LED lamp bead array (1) and the power supply (4);
lamp plate (2) set up the tip of shell (5), control circuit (3) set up in the inside of shell (5).
2. The LED infrared light device for in vitro cell culture according to claim 1, wherein the LED lamp bead array (1) comprises a plurality of parallel lamp bead groups, and each lamp bead group comprises a plurality of lamp beads (6) connected in series.
3. The LED infrared light device for in vitro cell culture according to claim 2, wherein the lamp bead group comprises a first lamp bead group and a second lamp bead group, the lamp panel (2) is a circular lamp panel (2), and the first lamp bead group is annularly arranged around the center of the circular lamp panel (2);
the second lamp bead group surrounds the first lamp bead group and is arranged in a circular shape.
4. The LED infrared light device for in vitro cell culture as claimed in claim 3, wherein the wavelength of the light formed by the LED bead array (1) is 760-980 nm.
5. According to the claimsThe LED infrared light device for in-vitro cell culture is characterized in that the light wave intensity formed by the LED lamp bead array (1) is 0.01J/cm2-80 J/cm2。
6. The LED infrared light device for in vitro cell culture according to claim 5, wherein the housing (5) is funnel-shaped.
7. The LED infrared light device for in vitro cell culture according to claim 6, wherein the lamp panel (2) is an aluminum lamp panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020640237.7U CN211976566U (en) | 2020-04-24 | 2020-04-24 | LED infrared light device for in vitro cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020640237.7U CN211976566U (en) | 2020-04-24 | 2020-04-24 | LED infrared light device for in vitro cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211976566U true CN211976566U (en) | 2020-11-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020640237.7U Active CN211976566U (en) | 2020-04-24 | 2020-04-24 | LED infrared light device for in vitro cell culture |
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| Country | Link |
|---|---|
| CN (1) | CN211976566U (en) |
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2020
- 2020-04-24 CN CN202020640237.7U patent/CN211976566U/en active Active
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Effective date of registration: 20230914 Address after: Room 206, Building 23, No. 298 Shaoshan North Road, Yuhua District, Changsha City, Hunan Province, 410007 Patentee after: Zeng Yuhui Address before: Room 227, 2nd Floor, Building 1, Zone 1, No. 8 Life Garden Road, Huilongguan Town, Changping District, Beijing, 100043 Patentee before: Beijing yannuo Biomedical Technology Co.,Ltd. |
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| TR01 | Transfer of patent right |