CN211955304U

CN211955304U - Elliptical opposite-insertion type array electrode and flexible detection system thereof

Info

Publication number: CN211955304U
Application number: CN202020290453.3U
Authority: CN
Inventors: 陶文艳; 邵敏华; 李金成
Original assignee: Guangzhou HKUST Fok Ying Tung Research Institute
Current assignee: Guangzhou HKUST Fok Ying Tung Research Institute
Priority date: 2019-12-31
Filing date: 2020-03-10
Publication date: 2020-11-17
Anticipated expiration: 2030-03-10

Abstract

The utility model provides an ellipse circular is to inserting type array electrode, including connecting a plurality of microstrip electrodes that are to inserting the type on two parallel lead wires, microstrip electrode's head end is convex. The utility model discloses ellipse shape is to inserting type array electrode and flexible detecting system thereof can be used to like the detection under the unconventional environment such as high pressure, high temperature, bending, folding, coiling, also can effectively improve the electric conductivity and collect efficiency to reach higher detectivity, and simultaneously detectable multicomponent material.

Description

Elliptical opposite-insertion type array electrode and flexible detection system thereof

Technical Field

The utility model belongs to the technical field of the electrochemistry detects technique and specifically relates to a type array electrode is inserted to ellipse and flexible detecting system thereof is related to.

Background

Electrochemistry is widely applied in the fields of chemical industry, metallurgy, metal corrosion prevention and protection and environmental science, and is a hot point of research at home and abroad. Electrochemical detection typically involves the use of various electrodes, such as working, auxiliary and reference electrodes. Among them, the shape, material and performance of the working electrode are important in electrochemical research.

In recent years, array microstrip electrodes have been widely used. Among the numerous types of array microstrip electrodes, the plug-in type array microstrip electrode (abbreviated as "IDA electrode") is the most attractive, the response current is large, the required detection instrument is simple, and different substances can be simultaneously detected on the two groups of electrodes to carry out 'generation-collection' feedback response, which is similar to a ring disk electrode and superior to the ring disk electrode. The substance generated by the collecting electrode in the IDA electrode can be diffused to the generating electrode for electrochemical reaction, so that the positive feedback effect is achieved, the response current of the generating electrode and the collecting electrode is increased, and the detection sensitivity can be improved. Most of the current common opposite-insertion type array microstrip electrode detection systems mainly use conventional rigid substrates such as quartz, glass, boron-doped diamond materials and the like, and are particularly applied to the field of microstrip electrode sensing. However, in recent years, the fields of flexible materials, artificial intelligence wearable devices and the like are becoming mature. Therefore, the design of a novel flexible plug-in array electrode detection system by taking a flexible material as a substrate has important significance for different application scenes (such as high pressure, high temperature, bending, folding, winding and the like).

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide an ellipse circular is to inserting type array electrode and flexible detecting system thereof can be used to like the detection under the unconventional environment such as high pressure, high temperature, bending, folding, coiling, also can effectively improve the electric conductivity and collection efficiency to reach higher detectivity, and simultaneously detectable multicomponent material.

In order to solve the technical problem, the utility model provides an ellipse circular is to inserting type array electrode, including connecting a plurality of microstrip electrodes to being the type of inserting on two parallel lead wires, microstrip electrode's head end is convex.

Preferably, the microstrip electrode has a length of 80 to 160 μm, a width of 8 to 16 μm, and a height of 0.1 to 0.5 μm.

Preferably, the distance between the adjacent microstrip electrodes is 4-8 μm.

The utility model also provides an use flexible detecting system of ellipse circular to inserting type array electrode, include the PDMS base and locate working electrode, auxiliary electrode, reference electrode on the PDMS base.

As a preferred mode, the working electrode comprises a plurality of pairs of oppositely inserted microstrip electrodes connected to two parallel lead wires, and the head ends of the microstrip electrodes are arc-shaped; the auxiliary electrode is approximately C-shaped and surrounds one side of the working electrode in a non-contact way; the reference electrode is inserted into the working electrode from the other side and is positioned between the two leads in parallel.

Preferably, the thickness of the PDMS substrate is 2-6 mm.

Preferably, the distances between the auxiliary electrode and the microstrip electrode and between the auxiliary electrode and the lead are respectively 2.5mm and 2.5 mm.

Preferably, the distances between the reference electrode and the microstrip electrode and between the reference electrode and the lead are respectively 2.5mm and 2.5 mm.

The utility model relates to an ellipse shape is to inserting type array electrode and flexible detecting system thereof compares with current design, and its advantage lies in: the utility model discloses use ellipse circular to inserting type array electrode, the head end of microstrip electrode is convex, detects the unimolecule in the fluid in the micron order passagewayIn the process, the surface resistance is greatly reduced, so that the detection sensitivity of the substance can be improved by about 3%, and a more effective detection system is provided for the detection of the monomolecular fluid in the micron channel field. The use of the flexible PDMS substrate enables the bending degree of the detection system to reach 360 degrees and the resistivity to be 100 omega cm²And the conduction detection can be realized. The flexible PDMS substrate and the arc-shaped head end of the flexible PDMS substrate are combined for use, so that the detection system can be used for detection under the unconventional environments such as high pressure, high temperature, bending, folding, winding and the like, and the electric conductivity and the collection efficiency can be effectively improved through the design of the size of the inserted array microstrip electrode and the thickness of the PDMS substrate, thereby achieving the purpose of high-sensitivity detection; and multiple component substances can be detected at the same time.

Drawings

Fig. 1 is a schematic view of the elliptical interdigitated array electrode of the present invention.

Fig. 2 is a schematic view of a single electrode of the elliptical opposite insertion type array electrode of the present invention.

Fig. 3 is a schematic diagram of the elliptical opposite insertion type array electrode-flexible detection system of the present invention.

FIG. 4 shows the detection of starch-rich peptides (A. beta.) in example 1 of the present invention₁₆) The inner graph is a linear fit curve.

Detailed Description

The invention is described in detail below with reference to the drawings and specific examples.

The utility model relates to an ellipse circular is to inserting type array electrode, and two rows of microstrip electrode 11 that a plurality of intervals set up are parallel to each other and alternate the range, form the comb type electrode to inserting the type, and microstrip electrode 11's head end is convex, and its tail end welding is on the lead wire 12 of two parallels. The length of each microstrip electrode 11 is 80-160 mu m, the width is 8-16 mu m, the height is 0.1-0.5 mu m, and the distance between every two adjacent microstrip electrodes 11 is 4-8 mu m.

The utility model discloses still relate to a use above-mentioned flexible ellipse shape to inserting type array electrode's flexible detecting system, as shown in FIG. 3, including PDMS basement 100 with locate working electrode 10, auxiliary electrode 20, reference electrode 30 that the PDMS basement arrived.

The PDMS substrate 100 is made of polydimethylsiloxane and has a thickness of 2-6 mm. The PDMS substrate 100 has certain flexibility, the degree of curling can reach 360 degrees, electrochemical signals can be output under the conditions of bending, folding and pressure, and meanwhile, the resistivity of the working electrode 10, the auxiliary electrode 20 and the reference electrode 30 on the PDMS substrate 100 is 100 omega cm²Hereinafter, the PDMS material is classified to have cytotoxicity of 0, which is more environmentally friendly.

The tail ends of the pairs of the inserted microstrip electrodes 11 are connected to a lead wire 12 as a working electrode 10.

The auxiliary electrode 20 is approximately C-shaped and surrounds one side of the working electrode 10 in a non-contact way; the distances between the auxiliary electrode 20 and the microstrip electrode 11 and between the auxiliary electrode and the lead 12 are respectively 2.5mm and 2.5 mm.

The reference electrode 30 is inserted into the position of the working electrode 10 close to the microstrip electrode 11 from the other side and is positioned between the two leads 12 in parallel; the distances between the reference electrode 30 and the microstrip electrode 11 and between the reference electrode and the lead 12 are respectively 2.5mm and 2.5 mm.

Make the utility model discloses detecting system can be used to like high pressure, high temperature, crooked, folding, the detection under the unconventional environment such as curl, the utility model discloses the collection efficiency of IDA electrode can reach more than 93%, is higher than the collection efficiency of traditional IDA electrode.

The method of measuring collection efficiency is as follows: first, cyclic voltammograms of the IDA electrode in the "generation-collection" mode were obtained. When the potential of the collecting electrode is-0.1V, the current of both the generating and collecting electrodes becomes large due to the presence of the positive feedback effect. Thus, the current i of the collecting electrode_CAnd current i of the generating electrode_GThe ratio of the two is defined as the collection efficiency.

Example 1

In this embodiment, the amyloid polypeptide (Abeta) as the primary screening biomarker for rapidly detecting Alzheimer's disease on line is used in the detection system (IDA-PDMS microfluidic chip system for short) with flexible elliptic opposite-insertion type array electrodes₁₆)。

By using matrix eliminationAgent (200. mu.g/mL bovine serum albumin, 0.5M sodium chloride, 500. mu.g/mL dextran and 0.5% Tween 20), A beta₁₆Can be detected in a serum environment of up to 75%. The Au-S bond of the self-assembly layer on the surface of the micro IDA electrode can be broken by high-temperature treatment at 150 ℃, so that the micro IDA-PDMS microfluidic chip system can be recycled.

Therefore, the IDA-PDMS microfluidic chip system in the embodiment has the advantages of reusability, quick response, micro sample consumption and the like. At the same time, we tested the IDA-microfluidic chip system for A beta in the concentration range of 0.025 mu M to 5.2 mu M₁₆In response, the results are shown in fig. 4, resulting in a linear fit equation: i.e. i_c(μA)＝27.87C_Aβ16(nM)+0.33(r＝0.990)。Aβ₁₆The linear range of (A) is 0.1 mu M to 2.8 mu M, which shows that the novel IDA-PDMS microfluidic chip system can be used for A beta in trace serum samples₁₆The method can be applied to clinically, quickly and effectively diagnose the senile dementia.

Claims

1. An ellipse shape is to inserting type array electrode which characterized in that: the micro-strip electrode structure comprises a plurality of pairs of oppositely-inserted micro-strip electrodes (11) connected to two parallel lead wires (12), wherein the head ends of the micro-strip electrodes (11) are arc-shaped.

2. An elliptically interdigitated array electrode according to claim 1, wherein: the length of the microstrip electrode (11) is 80-160 μm, the width is 8-16 μm, and the height is 0.1-0.5 μm.

3. An elliptically interdigitated array electrode according to claim 1 or 2, wherein: the distance between the adjacent microstrip electrodes (11) is 4-8 mu m.

4. A flexible detection system using an elliptic opposite insertion type array electrode is characterized in that: the device comprises a PDMS substrate (100), and a working electrode (10), an auxiliary electrode (20) and a reference electrode (30) which are arranged on the PDMS substrate (100).

5. A flexible detection system using an elliptical interdigitated array electrode as claimed in claim 4, wherein: the working electrode (10) comprises a plurality of pairs of oppositely-inserted microstrip electrodes (11) connected to two parallel leads (12), and the head end of each microstrip electrode (11) is arc-shaped; the auxiliary electrode (20) is approximately C-shaped and surrounds one side of the working electrode (10) in a non-contact way; the reference electrode (30) is inserted into the working electrode (10) from the other side and is positioned between the two leads (12) in parallel.

6. A flexible detection system using an elliptical interdigitated array electrode as claimed in claim 5, wherein: the thickness of the PDMS substrate (100) is 2-6 mm.

7. A flexible detection system using an elliptical interdigitated array electrode as claimed in claim 5, wherein: the distances between the auxiliary electrode (20) and the microstrip electrode (11) and between the auxiliary electrode and the lead (12) are respectively 2.5mm and 2.5 mm.

8. A flexible detection system using an elliptical interdigitated array electrode according to any one of claims 5 to 7, wherein: the distances between the reference electrode (30) and the microstrip electrode (11) and between the reference electrode and the lead (12) are respectively 2.5mm and 2.5 mm.