CN211947083U - Special liquid supplementing device for sterile cell culture bottle - Google Patents

Abstract

The utility model discloses a special fluid infusion device of aseptic formula cell culture bottle, include the lid with the bottleneck adaptation spiro union of culture medium storage bottle, the upper portion of lid is big end down's toper structure, has horizontal pivoted toper hood through connecting piece sealing connection on the open-top of lid, is provided with the drain pipe that is linked together with the inner chamber of lid on the lateral wall of toper hood, and the drain pipe slope upwards extends the setting, and the pipe diameter of its play liquid end is less than the bottleneck internal diameter of cell culture bottle. The utility model has the advantages of simple structure, simple installation and operation; the culture medium liquid with sufficient amount is directly supplemented safely and aseptically, the operation difficulty is reduced, the workload is reduced, and the human resources are saved; can realize multi-angle, multi-direction adjustment drain pipe, be convenient for make the liquid outlet of drain pipe stretch into the curved type bottleneck of cell culture bottle in, prevent to spatter and spill on table surface, avoid causing pollution and wasting of resources, make going on of experiment safety fast.

Description

Special liquid supplementing device for sterile cell culture bottle

Technical Field

The utility model belongs to the technical field of biological medicine and specifically relates to a special fluid infusion device of aseptic formula cell culture bottle is related to.

Background

At present, in laboratory experiments in the fields of biology, medicine and the like, especially in experiments for carrying out a large amount of in vitro culture on tumor cells, a culture medium liquid is often transferred from a culture medium storage bottle (i.e., a blue-mouth bottle) as shown in fig. 1 into a cell culture bottle as shown in fig. 2, so that the operation batches are more, continuous sterile transfer of the liquid needs to be carried out for multiple times, and the workload is very large; in addition, culture medium storage bottle bottleneck diameter is greater than the bottleneck diameter of cell culture bottle, and the bottleneck of cell culture bottle is mostly the curved opening, directly pours into culture medium liquid and not only appears spilling easily and leaks, can increase cell pollution's risk factor moreover, causes the wasting of resources, the experiment progress in direct influence later stage.

In the conventional pipetting process, a pipettor is mostly adopted for pipetting, and a gun head of the pipettor repeatedly enters and exits a culture bottle, so that the risk of cell pollution is increased; and the rifle head of pipettor has certain length, and the operation is extremely convenient and fast, and work efficiency is low, and the process that operating personnel made a round trip to shift causes hand excessive fatigue easily, and then the phenomenon of liquid feeding error appears.

Disclosure of Invention

The utility model aims to provide a simple structure, the special fluid infusion device of aseptic formula cell culture bottle of simple installation and easy and simple to handle

In order to achieve the above purpose, the utility model can adopt the following technical proposal:

special fluid infusion device of aseptic formula cell culture bottle, include the lid with the bottleneck adaptation spiro union of culture medium storage bottle, the upper portion of lid is big end down's toper structure there is horizontal pivoted toper hood through connecting piece sealing connection on the open-top of lid, be provided with on the lateral wall of toper hood with the drain pipe that the inner chamber of lid is linked together, the drain pipe slope upwards extends the setting, and its play liquid end's pipe diameter is less than the bottleneck internal diameter of cell culture bottle.

The connecting piece comprises an annular sliding chute horizontally arranged on the outer side surface of the opening at the top of the cover body along the circumferential direction, and an annular sliding block which is arranged on the inner side surface of the conical top cap and is in adaptive connection with the annular sliding chute; and an annular sealing ring which is respectively attached to the outer side wall of the cover body and the inner side wall of the conical top cap is arranged above the annular sliding groove.

The pipe diameter of the liquid outlet pipe is gradually reduced from the liquid inlet end to the liquid outlet end.

A sealing cover is movably buckled on the liquid outlet of the liquid outlet pipe.

The utility model has the advantages of simple structure, simple installation and operation. When a large amount of cell culture flasks are replenished, cell culture medium liquid can be poured easily and accurately through the device, so that sufficient culture medium liquid can be replenished safely and aseptically, the operation difficulty is reduced, the workload is reduced, and the human resources are saved; in addition, the drain pipe that upwards extends is supported in rotatable toper, can realize multi-angle, multi-direction adjustment drain pipe, and the liquid outlet that is convenient for make the drain pipe stretches into in the curved bottleneck of cell culture bottle, prevents to spatter and spills on table surface, avoids causing pollution and wasting of resources, makes the quick safe of experiment go on.

Drawings

FIG. 1 is a schematic view of a conventional medium storage bottle.

FIG. 2 is a schematic view of a conventional cell culture flask.

Fig. 3 is a schematic structural diagram of the present invention.

Fig. 4 is an enlarged sectional view of a portion a in fig. 3.

Detailed Description

As shown in figure 3, the special fluid infusion device for sterile cell culture bottle comprises a cover body 1 screwed with the bottle mouth of the culture medium storage bottle shown in figure 1, wherein the upper part of the cover body 1 is of a conical structure with a small upper part and a big lower part, thereby facilitating the outflow of the culture medium liquid.

A conical top cap 2 which rotates horizontally is connected to the top opening of the cover body 1 in a sealing manner through a connecting piece, specifically, as shown in fig. 4, the connecting piece can be an annular sliding groove 4 which is horizontally arranged on the outer side surface of the top opening of the cover body 1 along the circumferential direction, and an annular sliding block 5 which is arranged on the inner side surface of the conical top cap 2 and is in fit connection with the annular sliding groove 4; in addition, still can set up inside and outside respectively with the annular seal 6 of the laminating of lid 1 lateral wall and the inboard wall of toper top cap 2 in the top of annular spout 4 to strengthen this device's leakproofness, effectively avoid culture medium liquid to flow along the gap.

A liquid outlet pipe 3 communicated with the inner cavity of the cover body 1 is arranged on the side wall of the conical top cap 2, the culture medium liquid in the culture medium storage bottle flows out from the liquid outlet pipe 3, the liquid outlet pipe 3 is obliquely and upwardly extended, the pipe diameter of the liquid outlet pipe 3 is gradually reduced from a liquid inlet end to a liquid outlet end, and the pipe diameter of the liquid outlet end is smaller than the inner diameter of the bottle mouth of the cell culture bottle shown in fig. 2, so that the liquid outlet pipe can conveniently extend into the bottle mouth of the cell culture bottle; in addition, activity lock has sealed lid 7 on the liquid outlet of drain pipe 3, effectively avoids culture medium liquid to flow out when culture medium storage bottle emptys.

During the use, only need twist this device soon to the culture medium storage bottle bottleneck that corresponds on, then rotate toper hood 2 as required to adjustment drain pipe 3's angle takes off sealed lid 7, stretches into the liquid outlet of drain pipe 3 to the bottleneck of cell culture bottle in, later alright light topple over culture medium liquid, effectively prevent that culture medium liquid from spattering and spilling, avoid causing pollution and wasting of resources, realize safe, aseptic supplementary culture medium liquid.

Claims (5)

1. The utility model provides a special fluid infusion device of aseptic formula cell culture bottle which characterized in that: including lid (1) with the bottleneck adaptation spiro union of culture medium storage bottle, the upper portion of lid (1) is big end down's toper structure there is horizontal pivoted toper hood (2) through connecting piece sealing connection on the open-top of lid (1), be provided with on the lateral wall of toper hood (2) with drain pipe (3) that the inner chamber of lid (1) is linked together, drain pipe (3) slope upwards extends the setting, and the pipe diameter of its play liquid end is less than the bottleneck internal diameter of cell culture bottle.

2. The special fluid infusion device of aseptic formula cell culture bottle of claim 1, characterized in that: the connecting piece include along circumference level set up in annular spout (4) on lid (1) open-top lateral surface, and set up in annular slider (5) with this annular spout (4) adaptation connection on toper hood (2) medial surface.

3. The special fluid infusion device of aseptic formula cell culture bottle of claim 2, characterized in that: and an annular sealing ring (6) which is respectively attached to the outer side wall of the cover body (1) and the inner side wall of the conical top cap (2) is arranged above the annular sliding groove (4).

4. The special fluid infusion device of aseptic formula cell culture bottle of claim 1, characterized in that: the pipe diameter of the liquid outlet pipe (3) is gradually reduced from the liquid inlet end to the liquid outlet end.

5. The special fluid infusion device of aseptic formula cell culture bottle of claim 1, characterized in that: a sealing cover (7) is movably buckled on the liquid outlet of the liquid outlet pipe (3).

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