CN211927884U

CN211927884U - Water quality biotoxicity on-line monitoring device

Info

Publication number: CN211927884U
Application number: CN202020247327.XU
Authority: CN
Inventors: 郭翠莲; 孔祥峰; 程永强; 王阳; 王婧茹; 王昭玉; 马海宽; 孙中梁; 吕婧; 张丽; 邹妍; 高楠; 吕美蓉; 张艳敏
Original assignee: Oceanographic Instrumentation Research Institute Shandong Academy of Sciences
Current assignee: Oceanographic Instrumentation Research Institute Shandong Academy of Sciences; Institute of Oceanographic Instrumentation Shandong Academy of Sciences
Priority date: 2020-03-04
Filing date: 2020-03-04
Publication date: 2020-11-13
Anticipated expiration: 2030-03-04

Abstract

The utility model discloses a water quality biotoxicity online monitoring device, which comprises a six-channel selector valve, wherein any four side channels in the six-channel selector valve are respectively connected with a straight bag type filter, a refrigerated bacterial liquid bin, a temperature balance reaction unit and a buffer liquid reagent bottle through Teflon sample tubes, and a common channel of the six-channel selector valve is connected with an injection pump; the temperature balance reaction unit is connected with the photoelectric detection unit; the photoelectric detection unit comprises a darkroom, a detection cell and a photomultiplier, wherein the detection cell and the photomultiplier are installed in the darkroom, and the photomultiplier is installed below the detection cell and is connected with a data processing system. The method has the advantages that the luminous bacteria detection method is combined with flow injection, so that the volume and the reagent dosage of the detection system are reduced, and the monitoring efficiency is improved.

Description

Water quality biotoxicity on-line monitoring device

Technical Field

The application relates to a device for monitoring water quality biotoxicity on line based on luminous bacteria and sequential injection, belonging to the field of water quality monitoring.

Background

The environmental water pollution is increasingly serious, and the conventional chemical analysis method can only qualitatively or quantitatively test the components or the concentration of pollutants and cannot evaluate the comprehensive toxicity effect of the water. The bioanalytical algorithm uses the response of an organism to environmental changes to characterize the toxicity of a contaminant, allowing accurate assessment of the combined toxicity of a single contaminant or multiple contaminants. Among them, the luminescent bacteria method is an early and mature technique applied in the biological analysis method. The technology utilizes the characteristic that the luminous intensity changes linearly after the luminous bacteria contacts with toxic substances, and particularly combines the characteristic with a photoelectric technical means to prepare the water quality toxicity online monitoring system, so that the water quality safety can be evaluated quickly, and the defects of the traditional water quality evaluation method are overcome.

In the nineties, China also placed the Photorhabdus Law into national standards (GT/T15441-1995). However, the related domestic instruments are still relatively backward, the online monitoring instruments generally adopt a mechanical arm mode to suck samples and reagents, the appearance is cabinet type or wall-mounted, the online monitoring instruments are only limited to laboratory use, and the problems of large volume and power, poor timeliness, complex operation, high cost, short storage time of easily consumed luminescent bacteria, large dosage and the like generally exist. Based on the problems, the invention combines the luminous bacteria method with the sequential injection method, develops a device suitable for on-line monitoring of water quality biotoxicity in seawater or fresh water, and can carry out real-time on-line monitoring on comprehensive biotoxicity such as heavy metal, organic pollutant, pesticide residue, antibiotics and the like in water. The device is small in size, high in automation, simple in operation and capable of improving monitoring efficiency, and can be used for monitoring a water source or monitoring the inlet and outlet of a water plant.

Disclosure of Invention

In order to overcome the problems, the application provides an online water quality biotoxicity monitoring device based on luminous bacteria and sequential injection. The device has characteristics such as small, easy operation, automation height. The technical proposal is that the method comprises the following steps,

a water quality biotoxicity online monitoring device comprises six-channel selection valves, wherein any four side channels of the six-channel selection valves are respectively connected with a straight bag type filter, a refrigerated bacterial liquid bin, a temperature balance reaction unit and a buffer liquid reagent bottle through Teflon sample tubes, and a common channel of the six-channel selection valves is connected with an injection pump; the temperature balance reaction unit is connected with the photoelectric detection unit; the photoelectric detection unit comprises a darkroom, a detection cell and a photomultiplier, wherein the detection cell and the photomultiplier are installed in the darkroom, and the photomultiplier is installed below the detection cell and is connected with a data processing system.

Further, the refrigerated bacteria liquid bin comprises a shell made of ABS material, a semiconductor refrigerator inner container and a temperature controller; and a micro shaking table is arranged outside the refrigerated bacteria liquid bin.

Furthermore, the temperature balance reaction unit comprises a temperature control heating rod, the temperature is controlled between 15 and 25 ℃, a Teflon sample tube is wound on the temperature control heating rod, and the outer layer of the Teflon sample tube is wrapped by a heat insulation material.

Furthermore, a folding filter element is arranged in the straight bag type filter, and the aperture of a filter membrane of the straight bag type filter is 0.5-5 μm.

Furthermore, the detection pool is of a cuboid structure and made of quartz, a U-shaped channel is carved in the detection pool, and the U-shaped channel can contain 0.5-4mL of liquid to be detected.

Furthermore, the other two side channels in the six-channel selection valve are respectively connected with a cleaning solution reagent bottle and a quality control sample reagent bottle.

Advantageous effects

1. The luminous bacteria detection method is combined with flow injection, so that the volume and the reagent dosage of a detection system are reduced, and the monitoring efficiency is improved;

2. the water quality pre-filter with small volume and high filtering efficiency is arranged, so that manual operation procedures are reduced, and the automation procedure is high;

3. the micro bacteria liquid storage unit is provided with a refrigeration temperature control function, so that the activity of the bacteria liquid is ensured to the maximum extent;

4. the bacteria liquid storage unit is provided with a micro shaking table to ensure the uniformity of the bacteria liquid before reaction;

5. the photomultiplier is arranged at the lower side of the U-shaped channel, and the light receiving area is increased.

Drawings

FIG. 1 is a schematic structural diagram of the present application;

FIG. 2 shows the luminescence inhibition ratio and the heavy metal Zn measured by the present application²⁺A graph of concentration dependence;

wherein 1-straight bag type filter; 2-quality control sample reagent bottle; 3-cleaning liquid reagent bottle; 4-buffer solution reagent bottle; 5-six channel selector valve; 6-a syringe pump; 7-refrigerating the fungus liquid bin; 8-temperature equilibrium reactor; 9-U-shaped channel; 10-a photomultiplier tube; 11-darkroom; 12-waste liquid bottle; 13-a data processing system; 14-teflon sample tube; 15-detection cell.

Detailed Description

The following further description of the technology, in conjunction with the accompanying figures 1-2 and the specific embodiments, is provided to assist in understanding the present invention.

A water quality biotoxicity online monitoring device comprises a six-channel selection valve 5, any four side channels in the six-channel selection valve 5 are respectively connected with a straight bag type filter 1, a refrigerated bacteria liquid bin 7, a temperature balance reaction unit 8, a buffer solution reagent bottle 4, a quality control sample reagent bottle 2 and a cleaning liquid reagent bottle 3 through Teflon sample tubes 14, and a common channel of the six-channel selection valve is connected with an injection pump 6; a folding filter element is arranged in the straight bag type filter 1, and the aperture of a filter membrane is 0.5-5 mu m; the temperature balance reaction unit 8 is connected with the photoelectric detection unit; the temperature balance reaction unit 8 comprises a temperature control heating rod, the temperature is controlled to be 15-25 ℃, a Teflon sample tube 14 is wound on the temperature control heating rod, and the outer layer of the Teflon sample tube 14 is wrapped with a heat insulation material; the photoelectric detection unit comprises a darkroom 11, a detection pool 15 and a photomultiplier tube 10, wherein the detection pool 15 and the photomultiplier tube 10 are installed in the darkroom 11, the photomultiplier tube 10 is installed below the detection pool 9 and is connected with a data processing system 13, the detection pool 15 is of a cuboid structure and made of quartz, a U-shaped channel 9 is carved in the detection pool, the U-shaped channel 9 can contain 0.5-4mL of liquid to be detected, one port of the U-shaped channel 9 is connected with the temperature balance reaction unit 8, and the other port of the U-shaped channel 9 is connected with the waste liquid bottle 12.

The refrigerated bacteria liquid bin 7 comprises a shell made of ABS material, a liner of a semiconductor refrigerator and a temperature controller; a micro shaking table is arranged outside the refrigerated bacteria liquid bin 7.

An on-line monitoring method for water quality biotoxicity comprises the following steps:

cleaning the system with cleaning solution before the experiment begins, and correcting the system with quality control samples if positive control is needed; the quality control sample is prepared by dissolving zinc sulfate heptahydrate into 2% sodium chloride solution, EC₅₀The value is controlled to be 5-12 mg/L;

the specific experimental process is

S1, starting a micro shaking table for 2min, and shaking the bacterial liquid uniformly; starting a temperature control heating rod, and controlling the temperature to be 20 ℃;

s2, starting an injection pump, pumping 200 mu L of bacterial liquid to a temperature balance reaction unit, pumping 1.8mL of buffer solution to the temperature balance reaction unit, repeatedly pushing and pulling the injection pump for 10 times to promote the buffer solution to be completely mixed with the bacterial liquid, and pumping the buffer solution to a detection unit;

s3, the light intensity received by the photomultiplier at the moment is the original light intensity I of the bacterial liquid₀The photomultiplier is converted into an electric signal and transmitted to a data processing system;

s4, repeating the steps S1-S2, replacing the buffer solution in the step S2 with a filtered water sample to be detected, completely mixing the water sample with the bacterial solution, reacting for 15min under the same conditions, and pumping the mixture into a detection unit;

s5, the light intensity received by the photomultiplier at the moment is the light intensity I after the bacterial liquid reacts with the poison in the water_tThe photomultiplier is converted into an electric signal and transmitted to a data processing system;

and S6, calculating the light-emitting inhibition rate by the data processing system.

2 percent sodium chloride is used as a solvent, zinc sulfate solution with series concentration is prepared as a water quality sample, vibrio fischeri bacterial liquid is used as a detection liquid, and the measured luminescence inhibition rate and heavy metal Zn are measured²⁺The relationship of concentration is shown in figure 2.

By the same method, the comprehensive biotoxicity of heavy metals, organic pollutants, pesticide residues, antibiotics and the like in water can be monitored on line in real time.

Of course, the above description is not intended to limit the present technology, and the present technology is not limited to the above examples, and those skilled in the art should also make changes, modifications, additions or substitutions within the spirit and scope of the present application.

Claims

1. A water quality biotoxicity online monitoring device is characterized by comprising six-channel selection valves, wherein any four side channels of the six-channel selection valves are respectively connected with a straight bag type filter, a refrigerated bacteria liquid bin, a temperature balance reaction unit and a buffer solution reagent bottle through Teflon sample tubes, and a common channel of the six-channel selection valves is connected with an injection pump; the temperature balance reaction unit is connected with the photoelectric detection unit; the photoelectric detection unit comprises a darkroom, a detection cell and a photomultiplier, wherein the detection cell and the photomultiplier are installed in the darkroom, and the photomultiplier is installed below the detection cell and is connected with a data processing system.

2. The water quality biotoxicity online monitoring device according to claim 1, wherein the refrigerated bacterial liquid bin comprises a shell made of ABS material, a semiconductor refrigerator inner container and a temperature controller; and a micro shaking table is arranged outside the refrigerated bacteria liquid bin.

3. The on-line monitoring device for the biotoxicity of the water quality as claimed in claim 1, wherein the temperature balance reaction unit comprises a temperature control heating rod, the temperature is controlled between 15 and 25 ℃, a Teflon sample tube is wound on the temperature control heating rod, and a heat insulation material is wrapped on the outer layer of the Teflon sample tube.

4. The on-line monitoring device for the biotoxicity of water quality as claimed in claim 1, wherein the straight bag type filter is internally provided with a folding filter element, and the aperture of a filter membrane of the straight bag type filter is 0.5-5 μm.

5. A water quality biotoxicity online monitoring device as claimed in claim 1, wherein the detection tank is of a cuboid structure, is made of quartz, is internally provided with a U-shaped channel, and can contain 0.5-4mL of liquid to be detected.

6. The on-line monitoring device for the biotoxicity of water quality as claimed in claim 1, wherein the other two side channels in the six-channel selection valve are respectively connected with a cleaning solution reagent bottle and a quality control sample reagent bottle.