CN211865069U - Oxidase reagent dropping bottle - Google Patents

Abstract

The utility model belongs to the technical field of the microbiological test, specifically be an oxidase reagent dropping bottle. The oxidase reagent dropping bottle comprises a sealed plastic bottle, wherein one end of the plastic bottle is provided with a section of sealed communicating tubule, distilled water with a certain height is sealed in the sealed plastic bottle, the side wall of the plastic bottle is communicated with a section of plastic pipe, one end of the plastic pipe, which is far away from the plastic bottle, is in threaded connection with a bottle cap, the bottle cap comprises a lower cap ring and an upper cap, and a circle of tearable pull ring is connected between the lower cap ring and the upper cap; the device comprises an upper cover, a plastic pipe and a lower cover, wherein a first piston, a second piston and a push rod are arranged in the plastic pipe, the other end of the push rod is fixed on the lower surface of the upper cover, and a cavity for containing an oxidase reagent is formed between the first piston, the second piston and the inner wall of the plastic pipe. The oxidase reagent dropping bottle of the utility model has low cost, convenient and fast reagent preparation and experiment operation, effectively saves manpower and material resources, reduces the comprehensive cost, and can be popularized and applied to clinic.

Description

Oxidase reagent dropping bottle

[ technical field ] A method for producing a semiconductor device

The utility model belongs to the technical field of the microbiological test, concretely relates to oxidase reagent dropping bottle.

[ background of the invention ]

In the field of microbiology, it is generally necessary to perform oxidase tests on bacteria in order to rapidly identify bacteria of different species, for example for identifying Neisseria and Vibrio, distinguishing bacteria of the Enterobacteriaceae family from non-fermentable bacteria.

At present, the common methods for oxidase testing are:

(1) coating the cultured bacteria on filter paper, preparing an oxidase reagent into an aqueous solution with a certain concentration, dripping the solution on the bacterial colony of the filter paper, and observing the color change of the bacterial colony; (2) or firstly dropping the reagent on a small filter paper, then picking the bacterial colony and smearing the bacterial colony on the filter paper, and observing the color change of the bacterial colony; if the color is unchanged, the color is oxidase negative, and if the color is changed to rose red or blue, the color is oxidase positive.

The above method has the following disadvantages: (1) the preparation of the oxidase reagent needs to be carried out under aseptic conditions, and the preparation of the reagent has a plurality of influence factors, so that false positive or false negative results are easily caused. (2) The oxidase test has high requirements on the professional skills of operators, and the experimental vessels need to be sterile, so that the result is inaccurate due to the irregular operation.

Although the CN 101029873-oxidase test solution and the test tube thereof provide a commercial oxidase reagent, the new problem is brought, mainly after the oxidase reagent is prepared, the test result is inaccurate because the oxidase reagent is easy to oxidize under illumination, and the reagent is generally required to be prepared fresh every week. The pre-prepared commercial reagent cannot be prepared and used immediately, so that great risk is brought, the result is not accurate due to easy oxidative deterioration, and in order to eliminate the risk, a great deal of facilities and energy are required for preserving the reagent, so that resource waste is undoubtedly caused.

[ Utility model ] content

In view of the above, there is a need to provide an oxidase reagent dropping bottle to overcome the above technical problems.

In order to achieve the above purpose, the utility model adopts the technical proposal that:

an oxidase reagent dropping bottle comprises a sealed plastic bottle, wherein one end of the plastic bottle is provided with a section of sealed communicating tubule, distilled water with a certain height is sealed in the sealed plastic bottle, the side wall of the plastic bottle is communicated with a section of plastic pipe, one end of the plastic pipe, which is far away from the plastic bottle, is in threaded connection with a bottle cap, the bottle cap comprises a lower cap ring and an upper cap, and a circle of tearable pull ring is connected between the lower cap ring and the upper cap; the device comprises an upper cover, a plastic pipe, a first piston, a second piston and a push rod, wherein the plastic pipe is internally provided with the first piston, the second piston and the push rod, the first piston and the second piston are arranged in the plastic pipe in a sealing and sliding mode, the first piston is fixed at one end of the push rod and positioned at a pipe orifice of the plastic pipe, the second piston is fixed at the middle of the push rod, the other end of the push rod is fixed on the lower surface of the upper cover, and a cavity used for containing an oxidase reagent is formed among the first piston, the second piston and.

Further, the distance between the first piston and the second piston is equal to the height of the pull ring.

Further, the included angle between the plastic pipe and the axis of the plastic bottle is 15-90 degrees.

Furthermore, the plastic bottle and the thin tube are made of brown plastic materials.

Further, the plastic bottle is made of soft plastic.

Furthermore, a cover cap matched with the thin tube is sleeved on one end, far away from the plastic bottle, of the thin tube through threads.

The utility model discloses following beneficial effect has:

(1) the oxidase reagent dropping bottle contains an oxidase reagent and distilled water, and can be prepared by rotating the bottle cap, so that the preparation process is simplified, the preparation speed and the preparation quality of the reagent are improved, the preparation can be carried out immediately, and the accuracy of the test result of the oxidase is ensured.

(2) The oxidase reagent is dissolved and shaken uniformly in the dropping bottle, the cap is unscrewed during the test, a small section at the end part of the thin tube is cut off to form a dropping hole, then the plastic bottle is extruded, the reagent can be dripped out, the test is finished, the cap is screwed on, the cap can isolate the reagent from air, the oxidation is avoided, the reagent is convenient to store in a sealing way, the reagent can be used for multiple times, the operation of the whole process is simple, and the requirement on the professional technology of an operator is not high.

(3) The body of the oxidase dropping bottle is made of brown plastic, so that the problem of light oxidation is solved.

The utility model discloses an oxidase reagent dropping bottle is with low costs, and reagent is prepared and experiment convenient operation is swift, has effectively practiced thrift manpower, material resources, has reduced the comprehensive cost, can be to clinical popularization and application.

[ description of the drawings ]

Fig. 1 is a schematic structural view of a bottle cap in an unused state.

Fig. 2 is a partially enlarged view of a in fig. 1.

Fig. 3 is a schematic view of the construction of the closure after the ring pull has been torn and the cap has been tightened.

Detailed description of the embodiments reference is made to the accompanying drawings in which:

1. a plastic bottle; 2. a thin tube; 3. distilled water; 4. a bottle cap; 41. a lower cover ring; 42. an upper cover; 5. a pull ring; 6. a first piston; 7. a second piston; 8. a push rod; 9. an oxidase reagent; 10. and (7) capping.

The following detailed description of the invention will be further described in conjunction with the above-identified drawings.

[ detailed description ] embodiments

The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The oxidase reagent dropping bottle shown in fig. 1 and fig. 2 comprises a sealed plastic bottle 1, wherein one end of the plastic bottle 1 is provided with a section of sealed communicating tubule 2, distilled water 3 with a certain height is sealed in the sealed plastic bottle 1, the side wall of the plastic bottle 1 is communicated with a section of plastic tube, one end of the plastic tube, which is far away from the plastic bottle 1, is in threaded connection with a bottle cap 4, the bottle cap 4 comprises a lower cap ring 41 and an upper cap 42, and a circle of tearable pull ring 5 is connected between the lower cap ring 41 and the upper cap 42; a first piston 6, a second piston 7 and a push rod 8 are arranged in the plastic pipe, the first piston 6 and the second piston 7 are arranged in the plastic pipe in a sealing sliding mode, the first piston 6 is fixed at one end of the push rod 8 and located at the pipe orifice of the plastic pipe, the second piston 7 is fixed in the middle of the push rod 8, the other end of the push rod 8 is fixed to the lower surface of the upper cover 42, and a cavity used for containing an oxidase reagent 9 is formed among the first piston 6, the second piston 7 and the inner wall of the plastic pipe.

As shown in figure 2, the oxidase reagent 9 is pre-loaded into a cavity formed by the first piston 6, the second piston 7 and the inner wall of the plastic tube, at the moment, the first piston 6 seals the opening of the plastic tube, so that the oxidase reagent 9 is isolated from the distilled water 3 in the plastic bottle 1, and the oxidase reagent 9 is not affected with damp and deteriorated.

In this embodiment, the distance between the first piston 6 and the second piston 7 is equal to the height of the tab 5.

As shown in figure 3, when the pull ring 5 is torn off, the upper cover 42 can rotate downwards to push the oxidase reagent 9 into the plastic bottle 1, and because the distance between the first piston 6 and the second piston 7 is equal to the height of the pull ring 5, when the upper cover 42 is screwed to contact the lower cover ring 41, the second piston 7 is just positioned at the pipe orifice of the plastic pipe to seal the solution in the plastic bottle 1, so that the use process is prevented from being polluted.

In this embodiment, the included angle between the plastic tube and the axis of the plastic bottle 1 is 15-90 °, and the plastic tube and the axis of the plastic bottle 1 form a certain inclination angle, so that the oxidase reagent 9 can naturally fall into the plastic bottle 1, and the mixing operation can be smoothly completed.

In another embodiment of the present invention, the plastic bottle 1 and the plastic tube are made of brown plastic material, which can prevent the prepared solution from being oxidized by light.

In another embodiment of the present invention, the plastic bottle 1 is made of soft plastic material, so that the operator can squeeze the plastic bottle 1 to drip the reagent. One end of the thin tube 2 far away from the plastic bottle 1 is in threaded sleeve connection with a cap 10 matched with the thin tube 2, and when an operator cuts off a small section at the end of the thin tube 2 to form a dropping hole, the cap 10 is used for sealing the dropping hole to prevent a reagent in the dropping bottle from being oxidized.

In this example, the ratio of the oxidase reagent 9 to the distilled water 3 was 1g to 100 ml.

The utility model discloses the use as follows: tear pull ring 5 open, upper cover 42 rotates downwards, upper cover 42 drives push rod 8 and moves down, second piston 7 promotes in the cavity oxidase reagent 9 gets into plastic bottle 1, can suitably rock plastic bottle 1 this moment and make oxidase reagent 9 and distilled water 3 misce bene, unscrew block 10 after that, cut off a little of tubule 2 tip in order to form the drip hole, extrude plastic bottle 1 after that, can drip out the reagent, it drips on the bacterial colony that awaits measuring to get 1 drop of reagent, both can observe the result immediately, the test is ended, screw up block 10, block 10 can make the isolated air of reagent, avoid the oxidation, be convenient for sealed the saving, can repetitious usage, whole process operation is simple, it is not high to operating personnel's professional technical requirement.

The above description is for the detailed description of the preferred possible embodiments of the present invention, but the embodiments are not intended to limit the scope of the present invention, and all equivalent changes or modifications accomplished under the technical spirit suggested by the present invention should fall within the scope of the present invention.

Claims (6)

1. The utility model provides an oxidase reagent dropping bottle, is equipped with one section sealed intercommunication tubule (2) including sealed plastic bottle (1) in plastic bottle (1) one end, and the encapsulation has distilled water (3) of a take the altitude in sealed plastic bottle (1), its characterized in that: a section of plastic pipe is communicated with the side wall of the plastic bottle (1), one end of the plastic pipe, which is far away from the plastic bottle, is in threaded connection with a bottle cap (4), the bottle cap (4) comprises a lower cap ring (41) and an upper cap (42), and a circle of pull ring (5) which can be torn is connected between the lower cap ring (41) and the upper cap (42); have first piston (6), second piston (7) and a push rod (8) in the plastic tubing, first piston (6), second piston (7) seal up and slide and set up in the plastic tubing, first piston (6) are fixed the one end of push rod (8) is located plastic tubing pipe orifice department, second piston (7) are fixed the middle part of push rod (8), the other end of push rod (8) is fixed the lower surface of upper cover (42), constitute a cavity that is used for holding oxidase reagent (9) jointly between first piston (6), second piston (7) and the plastic tubing inner wall.

2. The oxidase reagent drip bottle of claim 1, wherein: the distance between the first piston (6) and the second piston (7) is equal to the height of the pull ring (5).

3. The oxidase reagent drip bottle of claim 1, wherein: the included angle between the plastic pipe and the axis of the plastic bottle (1) is 15-90 degrees.

4. The oxidase reagent drip bottle of claim 1, wherein: the plastic bottle (1) and the thin tube (2) are both made of brown plastic materials.

5. The oxidase reagent drip bottle of claim 1, wherein: the plastic bottle (1) is made of soft plastic.

6. The oxidase reagent drip bottle of claim 1, wherein: and one end of the thin tube (2) far away from the plastic bottle (1) is in threaded sleeve connection with a cap (10) matched with the thin tube (2).

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