CN211847894U - Cell-adhered PEGDA composite film - Google Patents

Abstract

The utility model discloses a cell adhesion's PEGDA composite film. The film comprises a core layer and an upper surface convex layer, wherein the core layer is formed by mixing a polyethylene glycol diacrylate hydrogel solution and a polystyrene nano-bead dispersion liquid and then curing the mixture after ultraviolet exposure, and the upper surface convex layer is formed by exposing the polystyrene nano-beads on the surface of the core layer. The utility model discloses well accessible changes the concentration of nanometer bobble dispersion, adjusts the surface morphology of solidification back aquogel composite film, roughness, the mechanical properties of aquogel film and the cell to the adhesion characteristic of film. The utility model discloses a film is with low costs, and adjusts the mechanical properties and the cell adhesion characteristic of film well.

Description

Cell-adhered PEGDA composite film

Technical Field

The utility model relates to a cell adhesion's PEGDA composite film relates to transportation, biosensor and the tissue scaffold field of medicine.

Background

Until now, tissue engineering faces a series of challenges, such as more efficient simulation of extracellular environment, and construction of more complex tissue structures. Therefore, hydrogels with controllable physical, chemical, or even biological properties have a positive driving effect on the construction of functional tissues in vitro. Polyethylene glycol diacrylate (PEGDA), a commonly used hydrogel, has been widely used in drug delivery, biosensors, and tissue scaffolds due to its good biocompatibility and excellent mechanical properties. However, the biological inertness of PEGDA, i.e., the non-adherent surface nature of cells, also greatly hinders its further use in tissue engineering.

The hydrogel films are typically treated with a mixed solution of carbodiimide and hydroxy thiosuccinimide (EDC/NHS), but this chemical approach is somewhat toxic to subsequent cell adhesion. Therefore, there is a need to obtain a film that can rapidly achieve the adhesion characteristics of polyethylene glycol diacrylate films without harm.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned weak point that exists among the prior art, PEGDA's biological inertia leads to the problem of cell non-adhesion and the problem of the toxicity that its chemical modification accompanies, the utility model discloses avoid the technical thought of chemical modification method, carry out physical structure design to PEGDA film through nanometer polystyrene bobble, obtain the PEGDA composite film that can effectively adhere the cell, obtain the adhesion effect to the cell through PEGDA composite film physical structure characteristic promptly, overcome the toxicity problem that chemical modification method brought.

In order to achieve the above object, the technical solution of the PEGDA composite film of the present invention is:

a cell-adherent PEGDA composite film comprising a core layer and a raised layer on an upper surface of the core layer, the raised layer adhered to the upper surface of the core layer.

Further, the following steps:

the core layer is formed by mixing polyethylene glycol diacrylate hydrogel solution and polystyrene nano-sphere dispersion liquid, then curing the mixture after ultraviolet exposure, and the convex layer on the upper surface of the core layer is formed by exposing polystyrene spheres on the surface of the core layer.

Further, the following steps: the mass ratio of the polyethylene glycol diacrylate hydrogel solution to the polystyrene pellets is 400-1000: 1.

And the mechanical property and the cell adhesion property of the cell adhesion PEGDA composite film can be realized by changing the concentration of the nano-bead dispersion liquid.

The utility model has the advantages as follows:

1. the utility model discloses cell adhesion's PEGDA composite film obtains the cell to this patent composite film's adhesion characteristic through the surface appearance, roughness, the hydrogel film's that change PEGDA composite film physical structure mechanical characteristic.

2. The cell-adhered PEGDA composite film of the utility model has low cost and low requirement on the environment.

3. The utility model discloses cell adhesion's PEGDA composite film has good biocompatibility, realizes the cell through composite film's physical structure characteristic and adsorbs, and is nontoxic, more green to the adhesion cell.

4. The utility model discloses cell adhesion's PEGDA composite film accessible changes the concentration of nanometer pellet, adjusts the surface morphology of solidification back aquogel composite film, roughness, the mechanical properties of aquogel film and the cell characteristic of adhering to the film.

Meanwhile, the present invention will be further explained with reference to the drawings and the following detailed description.

Drawings

FIG. 1 is a schematic structural view of a cell-adhered PEGDA composite membrane.

In the figure: 1-a raised layer; 2-core layer

FIG. 2 is a photograph of the adhesion fluorescence of cells to a PEGDA composite film to which various cells adhere;

fig. 2 (a) is a diagram showing cell adhesion of a pure hydrogel film, fig. 2 (B) is a diagram showing cell adhesion of a hydrogel film having a nanosphere dispersion concentration of 10%, fig. 2 (C) is a diagram showing cell adhesion of a hydrogel film having a nanosphere dispersion concentration of 20%, fig. 2 (D) is a diagram showing cell adhesion of a hydrogel film having a nanosphere dispersion concentration of 30%, fig. 2 (E) is a diagram showing cell adhesion of a hydrogel film having a nanosphere dispersion concentration of 40%, and fig. 2 (F) is a diagram showing cell adhesion of a hydrogel film having a nanosphere dispersion concentration of 50%.

Detailed Description

Examples

As shown in fig. 1, a cell-adhesive PEGDA composite film includes a core layer 2 and a bump layer 1 on an upper surface of the core layer, wherein the bump layer 1 is adhered to the upper surface of the core layer 2.

In this embodiment, the core layer 2 is formed by mixing a polyethylene glycol diacrylate hydrogel solution and polystyrene beads, and curing the mixture after ultraviolet exposure, and the raised layer 1 on the upper surface of the core layer is formed by exposing the polystyrene beads on the surface of the core layer 2.

The polyethylene glycol diacrylate hydrogel solution is prepared by mixing polyethylene glycol diacrylate, a photoinitiator TPO and 75% alcohol.

In this example, a process for preparing a cell-adherent PEGDA composite membrane is also provided, comprising the following steps:

1. and cleaning one glass slide twice by using absolute ethyl alcohol, and drying the cover glass by using nitrogen for later use.

2. Preparing a mixed solution of polyethylene glycol diacrylate (PEGDA) and a photoinitiator (TPO) by using 75% alcohol, wherein the final volume concentration of the polyethylene glycol diacrylate is 40%, the mass concentration of the photoinitiator TPO is 0.005g/mL, and then taking five prepared solutions, respectively adding a nano-bead dispersion liquid (the bead concentration of the dispersion liquid is 2.5 g/mL), and the volume concentrations of the five nano-bead dispersion liquids are 10%, 20%, 30%, 40% and 50%.

3. 0.5ml of the prepared solution was dropped onto the slide so that the solution was evenly spread on the surface of the slide.

4. Placing the glass slide on a frame, irradiating the glass substrate by ultraviolet light, inducing the mixed solution of the polyethylene glycol diacrylate, the photoinitiator and the nano-beads to perform a crosslinking reaction, and curing and adhering the mixed solution on the surface of the glass to form the composite hydrogel film.

The PEGDA composite membrane of the adherent cells of this example was tested for cell adhesion:

1. the five prepared composite films (the volume concentrations of the nano-bead dispersion solutions are 10%, 20%, 30%, 40% and 50%) and a pure polyethylene glycol diacrylate hydrogel film (control group) were placed in a cell culture dish, respectively.

2. Is divided intoSix culture dishes were added at a concentration of 3X 10⁵Cells per ml were placed in an incubator for one day.

3. It was observed by microscope that the cells did not have any adhesion property to the pure polyethylene glycol diacrylate hydrogel film (fig. 2A), the cell adhesion property became better as the concentration of the dispersion of the membrane-added nanospheres increased (fig. 2B-2E), and the cells almost covered the surface of the film when the concentration of the dispersion of the membrane-added nanospheres was 50% (fig. 2F).

Through the PEGDA composite film test of adhesion cell, the PEGDA composite film of this patent has the adhesion to the cell, moreover, the utility model discloses well accessible changes the concentration of nanometer bobble dispersion, adjusts the surface morphology of solidification back aquogel composite film, roughness, the mechanical properties of aquogel film and the cell characteristic of adhering to the film. Furthermore, the utility model discloses composite film's preparation simple process, it is with low costs, low to the environmental requirement. Moreover, the utility model discloses use polyethylene glycol diacrylate aquogel to have good biocompatibility.

The above-mentioned example is only used for explaining the utility model concept, and not to the limit of the utility model right protection, and all utilize this concept to be right the utility model carries out insubstantial change, all should fall into the scope of protection of the utility model.

Claims (1)

1. A cell-adhesive PEGDA composite film is characterized by comprising a core layer and a bump layer, wherein the bump layer is adhered to the upper surface of the core layer,

and the nano-scale polystyrene nano-spheres are exposed on the upper surface of the core layer to form the convex layer.

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