CN211800940U - Simple detachable capacity bottle rack - Google Patents

Simple detachable capacity bottle rack Download PDF

Info

Publication number
CN211800940U
CN211800940U CN201922360699.3U CN201922360699U CN211800940U CN 211800940 U CN211800940 U CN 211800940U CN 201922360699 U CN201922360699 U CN 201922360699U CN 211800940 U CN211800940 U CN 211800940U
Authority
CN
China
Prior art keywords
volumetric flask
positioning hole
plate
bottom plate
side plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201922360699.3U
Other languages
Chinese (zh)
Inventor
张莹婷
徐进
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Forestry University
Original Assignee
Nanjing Forestry University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Forestry University filed Critical Nanjing Forestry University
Priority to CN201922360699.3U priority Critical patent/CN211800940U/en
Application granted granted Critical
Publication of CN211800940U publication Critical patent/CN211800940U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Abstract

The utility model discloses a simple detachable capacity bottleholder belongs to biological assay equipment technical field. The capacity bottle rack comprises: the device comprises a first side plate, a second side plate, a first volumetric flask positioning hole sheet, a second volumetric flask positioning hole sheet, a bottom plate and a water collecting basin, wherein the two side plates are respectively connected with the two ends of the bottom plate at the bottom ends of the two side plates, so that the bottom plate is parallel to a horizontal plane, the two side plates are vertical to the horizontal plane, and the plate surfaces of the two side plates are parallel to each other, thereby forming a supporting structure of a volumetric flask rack; the middle upper parts of the two side plates are respectively connected with one ends of the two volumetric flask positioning hole sheets, so that the planes of the two volumetric flask positioning hole sheets are parallel to each other and parallel to the bottom plate; a water collecting basin is arranged below the bottom plate, and two ends of the water collecting basin are respectively arranged on the two side plates; the two volumetric flask positioning hole sheets are provided with a plurality of positioning holes, and the bottom plate is provided with a liquid leakage hole. The utility model discloses can store the waste liquid at the fixed volumetric flask of experiment operation in-process, can dismantle and accomodate, the practicality is strong.

Description

Simple detachable capacity bottle rack
Technical Field
The utility model relates to a biological assay equipment technical field, more specifically say, relate to a simple and easy detachable capacity bottleholder.
Background
Filtration is the most common practice for separating the solution from the precipitate in the experiment. When the mixture of solution and precipitate passes through a filter (e.g., filter paper), the precipitate remains on the filter and the solution flows through the filter into a receiving receptacle. In experiments such as measurement of soluble sugars after filtration, a volumetric flask is often used as a container for receiving a solution because a constant volume is often required. At present, in most experiments, divide the volumetric flask to open on the experiment table one by one, filter the constant volume in proper order, nevertheless often cause the volumetric flask to be linked and is knocked over because of the funnel is overweight, the mistake of hand bumps the operation in the operation process to pollute the desktop, influence the process of experiment. Second, laboratory space is limited. Therefore, a simple detachable volumetric flask rack needs to be developed to prevent the volumetric flasks from being overturned in a chain and facilitate storage.
SUMMERY OF THE UTILITY MODEL
In view of the above problems in the prior art, the present invention provides a volumetric flask holder for holding volumetric flasks.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a simple detachable capacity bottle rack comprises: two side plates, two volumetric flask positioning hole sheets, a bottom plate and a water collecting basin; the two side plates comprise a first side plate and a second side plate, and the two volumetric flask positioning hole pieces comprise a first volumetric flask positioning hole piece and a second volumetric flask positioning hole piece; the two side plates are respectively connected with the two ends of the bottom plate at the bottom ends of the two side plates, so that the bottom plate is parallel to the horizontal plane, the two side plates are vertical to the horizontal plane, and the plate surfaces of the two side plates are parallel to each other, thereby forming a supporting structure of the capacity bottle rack; the middle upper parts of the two side plates are respectively connected with one ends of the two volumetric flask positioning hole sheets, so that the planes of the first volumetric flask positioning hole sheet and the second volumetric flask positioning hole sheet are parallel to each other and the bottom plate; a water collecting basin is arranged below the bottom plate, and two ends of the water collecting basin are respectively arranged on the two side plates; a plurality of positioning holes are formed in the two volumetric flask positioning hole sheets; and the bottom plate is provided with a liquid leakage hole.
Preferably, the width of the first side plate, the width of the second side plate, the width of the first volumetric flask positioning hole piece, the width of the second volumetric flask positioning hole piece, the width of the bottom plate and the width of the water collecting basin are the same, and the structure and the size of the first side plate and the second side plate are completely the same.
Preferably, the positioning holes of the two volumetric flask positioning hole pieces are circular, the two volumetric flask positioning hole pieces are arranged in a row, and each volumetric flask positioning hole piece is composed of parts which are divided into two parts from the middle diameter of each row of circular positioning holes along the long side of the volumetric flask positioning hole piece.
Preferably, the length, width, height and the position of connection of the first side plate, the second side plate, the first volumetric flask positioning hole sheet, the second volumetric flask positioning hole sheet, the bottom plate and the water collecting basin of the volumetric flask rack are customized according to the size specification of the volumetric flasks.
Preferably, the two side plates are provided with hollowed-out hand grasping holes.
Preferably, the first side plate and the second side plate are detachably connected with the first volumetric flask positioning hole piece, the second volumetric flask positioning hole piece, the bottom plate and the water collecting basin respectively.
Preferably, the first side plate and the second side plate are respectively detachably connected with the water collecting basin in a specific mode that: the inner side surfaces above the bottoms of the first side plate and the second side plate are respectively provided with two notches which are separated from each other and are parallel to the bottom edge, two short edges of the water collecting basin are respectively provided with two slots which correspond to the two notches, and the slots are protruded to be inserted into the two notches, so that the first side plate, the second side plate and the water collecting basin are connected.
Preferably, first curb plate, second curb plate respectively with first volumetric flask location hole piece, second volumetric flask location hole piece and the bottom plate can dismantle the connection concrete mode do: a plurality of sliding grooves are respectively arranged on the inner side surfaces of the first side plate and the second side plate, the length of each sliding groove is equal to the width of each side plate, the sliding grooves are parallel to the bottom edges of the two side plates, and the sliding grooves are positioned at the middle upper parts of the two side plates; the bottom of the sliding groove is wider than the front opening of the sliding groove; first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate all have the arch with the both ends that two curb plates are connected, and is bigger than first volumetric flask locating hole piece, second volumetric flask locating hole piece and end plate thickness, and bellied size and first volumetric flask locating hole piece, second volumetric flask locating hole piece and end plate thickness can make first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate pass through arbitrary opening at sliding tray both ends slides in the sliding tray inside to can not take out from the front opening of sliding tray, make first curb plate, second curb plate be connected with first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate respectively.
Preferably, the openings at the two ends of the sliding groove are further provided with a lock catch, and the specific structure of the lock catch is as follows: install one in the open-ended side below in both ends of sliding tray can the axle with the rectangular iron sheet of the side parallel rotation of first curb plate, second curb plate, install the magnetic sheet above the open-ended side in both ends of sliding tray, when rectangular iron sheet rotatory to magnetic sheet department, can by the magnetic sheet attracts and fixes, seals the tip opening of sliding tray.
Preferably, the bottom of the sliding groove is provided with a stainless steel gasket.
Has the advantages that: compared with the prior art, the utility model has the advantages that:
the utility model can fix the volumetric flask in the experimental operation process, and prevent the volumetric flask from being overturned or the funnel from being too heavy to cause integral unbalance so as to influence the experiment; secondly, the volumetric flask rack can be disassembled and assembled for containing volumetric flasks or being folded, so that the space is saved; in addition, the water accumulating basin can store waste liquid in the process of airing volumetric flasks, test tubes and experiments, and the tabletop is prevented from being polluted.
Drawings
FIG. 1 is a schematic view of a volumetric flask;
fig. 2 is a front view of the present invention;
fig. 3 is a diagram of the positioning hole sheet of the present invention, the positioning hole is divided into three rows and two columns, which are composed of A, B, C, D four parts, and there are 30 positioning holes;
fig. 4 is a diagram of the positioning hole sheet of the present invention, the positioning hole is divided into three rows and two columns, and is composed of four parts A, B, C, D, and there are 24 positioning holes;
fig. 5 is a diagram of the positioning hole sheet of the present invention, the positioning holes are divided into two rows and two columns, which are composed of A, B, C three parts, and there are 12 positioning holes;
fig. 6 is a schematic view of a bottom plate of the present invention;
FIG. 7 is a schematic view of the side plate and the hand grasping hole on the side plate of the present invention;
FIG. 8 is a schematic view of the water basin of the present invention;
fig. 9 is a schematic view of a side plate three-dimensional structure of the present invention;
fig. 10 is a schematic side view of the positioning hole plate or the bottom plate of the volumetric flask of the present invention.
Detailed Description
The present invention will be further described with reference to the following specific embodiments.
Example 1
Fig. 1 shows a schematic view of the volumetric flask.
Fig. 2-10 are schematic structural views of a simple detachable bottle rack, which includes: two side plates, two volumetric flask positioning hole sheets, a bottom plate 5 and a water collecting basin 6; the two side plates comprise a first side plate 1 and a second side plate 2, and the two volumetric flask positioning hole pieces comprise a first volumetric flask positioning hole piece 3 and a second volumetric flask positioning hole piece 4; the two side plates are respectively connected with the two ends of the bottom plate 5 at the bottom ends thereof, so that the bottom plate 5 is parallel to the horizontal plane, the two side plates are vertical to the horizontal plane, and the plate surfaces of the two side plates are parallel to each other, thereby forming a supporting structure of the capacity bottle rack; the middle upper parts of the two side plates are respectively connected with one ends of the two volumetric flask positioning hole sheets, so that the planes of the first volumetric flask positioning hole sheet 3 and the second volumetric flask positioning hole sheet 4 are parallel to each other and the bottom plate 5; a water collecting basin 6 is arranged below the bottom plate 5, and two ends of the water collecting basin 6 are respectively arranged on the two side plates; a plurality of positioning holes 7 are formed in the two volumetric flask positioning hole sheets; the bottom plate 5 is provided with a liquid leakage hole 8.
The width of the first side plate 1, the second side plate 2, the first volumetric flask positioning hole piece 3, the second volumetric flask positioning hole piece 4, the bottom plate 5 and the water collecting basin 6 is the same, and the structure and the size of the first side plate 1 and the second side plate 2 are completely the same.
The positioning holes 7 of the two volumetric flask positioning hole sheets are circular, the two volumetric flask positioning hole sheets are arranged in a row and column manner, and the two volumetric flask positioning hole sheets are composed of parts which are divided into two parts from the middle diameter part of the circular positioning hole 7 of each row along the long edge of the two volumetric flask positioning hole sheets.
The length, width, height and the positions of the first side plate 1, the second side plate 2, the first volumetric flask positioning hole sheet 3, the second volumetric flask positioning hole sheet 4, the bottom plate 5 and the water collecting basin 6 which are connected with each other of the volumetric flask rack are customized according to the size and specification of the volumetric flasks. For example: setting a first volumetric flask positioning hole piece and a second volumetric flask positioning hole piece to be 60cm long and 21.5cm in total width, wherein the diameter of a positioning hole of the first volumetric flask positioning hole piece is 2.5cm, and the distance from the positioning hole to the bottom plate is 10 cm; the diameter of the positioning hole piece of the second volumetric flask is 2cm, and the distance from the positioning hole piece to the bottom plate is 6cm, so that the requirements of volumetric flasks with different specifications, such as 20mL, 50mL, 100mL, 200mL and 250mL, can be met.
The two side plates are also provided with hollowed-out hand-grasping holes.
The first side plate 1 and the second side plate 2 are detachably connected with the first volumetric flask positioning hole piece 3, the second volumetric flask positioning hole piece 4, the bottom plate 5 and the water basin 6 respectively.
First curb plate 1, second curb plate 2 do with the water basin 6 can dismantle the connection concrete mode respectively: at the medial surface of first curb plate 1 and second curb plate 2 bottom top, all have two notches 10 that part from each other and be parallel with the base, be equipped with two slot archs 11 that correspond two notches respectively at two minor faces of ponding basin 6 and be arranged in inserting two notches 10, and make first curb plate 1, second curb plate 2 and ponding basin 6 be connected.
First curb plate 1, second curb plate 2 do respectively with first volumetric flask location hole piece 3, second volumetric flask location hole piece 4 and bottom plate 5 dismantled the connection concrete mode: the inner side surfaces of the first side plate 1 and the second side plate 2 are respectively provided with a plurality of sliding grooves 12, the length of each sliding groove 12 is equal to the width of each side plate, the sliding grooves are parallel to the bottom edges of the two side plates, and the sliding grooves are positioned at the middle upper parts of the two side plates; the bottom of the sliding groove 12 is wider than the front opening of the sliding groove; first volumetric flask locating hole piece 3, second volumetric flask locating hole piece 4 and bottom plate 5 all have arch 13 with the both ends that two curb plates are connected, and is bigger than first volumetric flask locating hole piece 3, second volumetric flask locating hole piece 4 and bottom plate 5's thickness to protruding 13's size and first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate thickness can make first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate pass through arbitrary opening at sliding tray 12 both ends slides in inside sliding tray 12 to can not take out from the positive opening of sliding tray, make first curb plate, second curb plate be connected with first volumetric flask locating hole piece, second volumetric flask locating hole piece and bottom plate respectively.
The both ends opening part of sliding tray 12 still is provided with hasp 14, and the concrete structure of hasp 14 is: a long iron sheet 17 which can rotate around a shaft 15 and is parallel to the side edges of the first side plate and the second side plate is arranged below the side surfaces of the openings at the two ends of the sliding groove 12, a magnetic sheet 16 is arranged above the side surfaces of the openings at the two ends of the sliding groove 12, and when the long iron sheet 17 rotates to the position of the magnetic sheet 16, the long iron sheet can be attracted and fixed by the magnetic sheet 16 to seal the opening at the end part of the sliding groove 12. The bottom of the sliding groove 12 is also provided with a stainless steel gasket.
The method for installing and using the capacity bottle rack comprises the following steps: connecting a water basin 6 with two side plates through a notch 10, connecting a bottom plate 5 with the two side plates through a sliding groove 12, determining the specification and the quantity of volumetric flasks, if 18 volumetric flasks of 50mL are needed, connecting A, B, C, D sheets in a volumetric flask positioning hole sheet shown in FIG. 4 with the side plates through the sliding groove 12 in the middle of the side plates in sequence, firstly putting an A sheet, then putting 6 volumetric flasks (the long neck of each volumetric flask is arranged in 1 positioning hole), then putting a B sheet, then putting 6 volumetric flasks, then putting a C sheet, then putting 6 volumetric flasks, finally putting a D sheet, combining A, B, C, D sheets together, then fixing the volumetric flasks in the positioning holes on a volumetric flask rack, placing the bottoms of the volumetric flasks on the bottom plate 5, putting a stainless steel gasket (the stainless steel gasket is thin and elastic and has the same length and width as the bottoms of the sliding grooves) at the bottom of the sliding grooves, make the volumetric flask locating hole piece of volumetric flask frame and the connection between two curb plates firm not rock, then buckle 14, the fixed volume bottleholder for the constant volume operation. After the constant volume is finished, the lock catch is firstly opened, A, B, C, D sheets are taken out and part of the volumetric flasks are taken out according to the reverse order of putting in, A, B, C, D sheets are installed on the volumetric flask rack, the lock catch 14 is buckled, the washed volumetric flasks can be reversely buckled on the volumetric flask rack and are fixed by the volumetric flask positioning hole sheets, residual water flows into the water collecting basin 7 through the liquid leakage hole 8 of the bottom plate 5, and the test tubes which are just washed clean can be reversely buckled and inserted into the positioning holes 7 to be aired on the volumetric flask rack. When the capacity bottle rack is not needed to be used, all parts can be detachably stored, and the capacity bottle rack is practical and convenient.

Claims (10)

1. The utility model provides a simple and easy detachable capacity bottleholder which characterized in that includes: two side plates, two volumetric flask positioning hole sheets, a bottom plate (5) and a water collecting basin (6); the two side plates comprise a first side plate (1) and a second side plate (2), and the two volumetric flask positioning hole sheets comprise a first volumetric flask positioning hole sheet (3) and a second volumetric flask positioning hole sheet (4); the two side plates are respectively connected with the two ends of the bottom plate (5) at the bottom ends thereof, so that the bottom plate (5) is parallel to the horizontal plane, the two side plates are vertical to the horizontal plane, and the plate surfaces of the two side plates are parallel to each other, thereby forming a supporting structure of the capacity bottle rack; the middle upper parts of the two side plates are respectively connected with one ends of the two volumetric flask positioning hole sheets, so that the planes of the first volumetric flask positioning hole sheet (3) and the second volumetric flask positioning hole sheet (4) are parallel to each other and the bottom plate (5); a water collecting basin (6) is arranged below the bottom plate (5), and two ends of the water collecting basin (6) are respectively arranged on the two side plates; a plurality of positioning holes (7) are formed in the two volumetric flask positioning hole sheets; and the bottom plate (5) is provided with a liquid leakage hole (8).
2. The simple detachable volumetric flask holder according to claim 1, wherein the first side plate (1), the second side plate (2), the first volumetric flask positioning hole piece (3), the second volumetric flask positioning hole piece (4), the bottom plate (5) and the water collecting basin (6) have the same width, and the first side plate (1) and the second side plate (2) have the same structure and size.
3. The rack as claimed in claim 1, wherein the positioning holes (7) of the two volumetric flask positioning hole pieces are circular, are arranged in a matrix, and are each composed of two parts along the long side thereof from the middle diameter of each row of circular positioning holes (7).
4. The simple detachable volumetric flask holder according to claim 1, wherein the length, width, height and the connecting position of the first side plate (1), the second side plate (2), the first volumetric flask positioning hole piece (3), the second volumetric flask positioning hole piece (4), the bottom plate (5) and the water basin (6) are customized according to the size and specification of the volumetric flasks.
5. The simple detachable capacity vase stand of claim 1, wherein the two side plates are provided with hollow hand-holding holes (9).
6. The simple detachable volumetric flask holder according to any one of claims 1 to 5, wherein the first side plate (1) and the second side plate (2) are detachably connected with the first volumetric flask positioning hole piece (3), the second volumetric flask positioning hole piece (4), the bottom plate (5) and the water collecting basin (6) respectively.
7. The simple detachable volumetric flask holder according to claim 6, wherein the detachable connection between the first side plate (1) and the water basin (6) and the detachable connection between the second side plate (2) and the water basin are as follows: at the medial surface of first curb plate (1) and second curb plate (2) bottom top, all have two to part each other and with two notches (10) that the base is parallel, be equipped with two correspondences respectively at two minor faces of ponding basin (6) the slot arch (11) of two notches are arranged in inserting two notches (10), and make first curb plate (1), second curb plate (2) can be connected with ponding basin (6).
8. The simple detachable volumetric flask holder according to claim 6, wherein the detachable connection between the first side plate (1) and the second side plate (2) and the first volumetric flask positioning hole piece (3), the second volumetric flask positioning hole piece (4) and the bottom plate (5) is as follows: a plurality of sliding grooves (12) are respectively arranged on the inner side surfaces of the first side plate (1) and the second side plate (2), the length of each sliding groove (12) is equal to the width of each side plate, the sliding grooves are parallel to the bottom edges of the two side plates, and the sliding grooves are positioned at the middle upper parts of the two side plates; the bottom of the sliding groove (12) is wider than the front opening of the sliding groove (12); the two ends of the first volumetric flask positioning hole sheet (3), the second volumetric flask positioning hole sheet (4) and the bottom plate (5) which are connected with the two side plates are respectively provided with a bulge (13) which has larger thickness than the first volumetric flask positioning hole sheet (3), the second volumetric flask positioning hole sheet (4) and the bottom plate (5), and the size of the bulge (13) and the thickness of the first volumetric flask positioning hole sheet (3), the second volumetric flask positioning hole sheet (4) and the bottom plate (5) can ensure that the first volumetric flask positioning hole sheet (3), the second volumetric flask positioning hole sheet (4) and the bottom plate (5) can slide into the sliding groove (12) through any opening at the two ends of the sliding groove (12), and the first side plate (1) and the second side plate (2) are respectively connected with the first volumetric flask positioning hole sheet (3), the second volumetric flask positioning hole sheet (4) and the bottom plate (5) because the first side plate cannot be drawn out from the front opening of the sliding groove (12).
9. The simple detachable capacity bottle rack as claimed in claim 8, wherein the openings at both ends of the sliding groove (12) are further provided with a lock catch (14), and the specific structure of the lock catch (14) is as follows: install one in the both ends open-ended side below of sliding tray (12) can pivot (15) with rectangular iron sheet (17) of the parallel rotation of side of first curb plate (1), second curb plate (2), install magnetic sheet (16) in the both ends open-ended side top of sliding tray (12), when rectangular iron sheet (17) rotatory to magnetic sheet (16) department, can by magnetic sheet (16) attract and fix, seal the tip opening of sliding tray (12).
10. The simple detachable capacity bottle rack as claimed in claim 9, wherein the bottom of the sliding groove (12) is provided with a stainless steel gasket.
CN201922360699.3U 2019-12-24 2019-12-24 Simple detachable capacity bottle rack Expired - Fee Related CN211800940U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201922360699.3U CN211800940U (en) 2019-12-24 2019-12-24 Simple detachable capacity bottle rack

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201922360699.3U CN211800940U (en) 2019-12-24 2019-12-24 Simple detachable capacity bottle rack

Publications (1)

Publication Number Publication Date
CN211800940U true CN211800940U (en) 2020-10-30

Family

ID=73033128

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201922360699.3U Expired - Fee Related CN211800940U (en) 2019-12-24 2019-12-24 Simple detachable capacity bottle rack

Country Status (1)

Country Link
CN (1) CN211800940U (en)

Similar Documents

Publication Publication Date Title
US3956125A (en) Filtration apparatus
US6254396B1 (en) Teaching device for science experiments
CN211800940U (en) Simple detachable capacity bottle rack
CN204841776U (en) Portable test -tube rack
CN207704710U (en) A kind of physics teaching buoyancy demonstration equipment
CN206897444U (en) A kind of chemical laboratory table
CN208038450U (en) A kind of multifunction combined gene detecting kit
CN205413110U (en) Small -size EP pipe holds device
CN205965916U (en) Used liquid -transfering gun frame in animal remedy measurement experiment room
CN203184050U (en) Novel test tube rack
CN208043631U (en) Cell rack
CN208695068U (en) Medical science inspection test-tube rack
CN217674134U (en) Multicolor PCR tube placing rack for reagent filling
CN107377030B (en) Laboratory is with three-dimensional volumetric flask rack
CN217132890U (en) Viscometer
CN111521470A (en) Folding dyeing rack, horizontal incubation box and using method
CN208494298U (en) Mobile chemical test operation platform
CN203916717U (en) A kind of Multifunctional test tube is taken in basket
CN217288509U (en) EP pipe support of convenient split and combination
CN211801025U (en) Endocrinology test-tube rack
CN219156888U (en) Tumor cell culture device for anti-tumor experiment
CN208308842U (en) A kind of culture dish rack
CN209123964U (en) A kind of glass bar rest stand easy to disassemble
CN209360522U (en) A kind of paper towel box easy to use
CN211800953U (en) Chemistry experiment room suction ear ball storage device

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20201030

Termination date: 20211224

CF01 Termination of patent right due to non-payment of annual fee