CN111521470A - Folding dyeing rack, horizontal incubation box and using method - Google Patents
Folding dyeing rack, horizontal incubation box and using method Download PDFInfo
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- CN111521470A CN111521470A CN202010235598.8A CN202010235598A CN111521470A CN 111521470 A CN111521470 A CN 111521470A CN 202010235598 A CN202010235598 A CN 202010235598A CN 111521470 A CN111521470 A CN 111521470A
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- 238000011534 incubation Methods 0.000 title claims abstract description 23
- 238000004043 dyeing Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title abstract description 18
- 238000005406 washing Methods 0.000 claims abstract description 7
- 239000011521 glass Substances 0.000 claims description 15
- 238000010186 staining Methods 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 210000003205 muscle Anatomy 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 230000002055 immunohistochemical effect Effects 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
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- 238000011532 immunohistochemical staining Methods 0.000 description 3
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- 238000002360 preparation method Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
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- 102000039446 nucleic acids Human genes 0.000 description 1
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention provides a folding dyeing rack, a horizontal incubation box and a using method. The method realizes the quick switching of the horizontal incubation and the vertical repairing and washing processes in the immunohistochemical experiment process, saves time, is convenient and fast to operate, and improves the treatment efficiency of the sample.
Description
Technical Field
The invention relates to the technical field of medical treatment, in particular to a folding dyeing rack, a horizontal incubation box and a using method.
Background
Immunohistochemical staining (ihc) refers to the binding of fluorescent or chromogenic chemical substances to antibodies, and the detection of the presence of a target antigen in cells or tissues by means of specific binding reactions between antigen and antibody in the immunological principle, which can be used not only to measure the amount of antigen expressed but also to observe the location of the antigen expressed. Any substance capable of binding an antibody, that is, an antigenic substance including a protein, a nucleic acid, a polysaccharide, a pathogen, etc., can be detected.
Immunohistochemistry has advantages of specificity, sensitivity, simplicity, rapidity and low cost, so it is widely used in hospitals and usually screens cancer by means of specific tumor markers. Immunohistochemical staining is a very important method for basic research, prevention and diagnosis.
In immunohistochemical staining, tissues need to be subjected to the steps of slicing, pasting, dewaxing, repairing, sealing, primary antibody incubation, secondary antibody incubation, staining, counterstaining, dehydration, transparentization, mounting and the like, a slide needs to be repeatedly taken out of a slide rack and flattened, and the slide rack is inserted to wash after operation. The use of existing slide holders has the following disadvantages: easy to scrape tissue; the position is easy to be misplaced; too many slides are flattened at one time, and the firstly flattened slides are easy to dehydrate and dry and generate non-specificity; the requirement on the operation proficiency is high; in cases where higher demands are made on the operating speed when the sample size is larger, there is also the possibility of missing reagent.
In addition, in the process of adding reagent for incubation after the slide is flattened, the incubation box needs to be in a horizontal humid environment, so that liquid is prevented from being gathered at the lower end due to the action of gravity, and the possibility of drying the slide is avoided at the other end. It is of course possible to avoid this problem by adding more reagent, but sometimes it is desirable to have the reagent spread on the slide when the reagent is less abundant.
In view of the foregoing, there is a need for a slide rack that can be quickly flattened and retracted, and an incubation cassette that can be mated to maintain the slide horizontal.
Disclosure of Invention
The invention provides a folding staining rack, a horizontal incubation box and a using method, which realize the quick switching of the horizontal incubation and the vertical repairing and washing processes in the immunohistochemistry experiment process, save time, are convenient to operate and improve the sample processing efficiency.
The technical scheme of the invention is realized as follows: the utility model provides a folding dyeing frame, includes a plurality of cardboard, and a plurality of cardboard is the zigzag folding, is provided with folding first connecting piece between the adjacent cardboard, is provided with the dead slot that is used for holding the slide on the cardboard, and the rear end interval of dead slot is provided with the horizontal muscle that is used for supporting the slide, and both ends all are provided with the separation blade group that is used for blocking the slide around the dead slot, and separation blade group includes two baffles, and two baffles are located the both sides of dead slot lower extreme respectively.
Further, a folding dyeing frame still includes two arm-plates, and a plurality of cardboard is arranged in between two arm-plates, and the arm-plate passes through the collapsible continuous of second connecting piece with adjacent card, and first connecting piece and second connecting piece are the hinge that has the pivot.
Furthermore, both sides of the upper end of each clamping plate are provided with magnetic sheets, and the attraction of the adjacent clamping plates is realized through the magnetic sheets.
Furthermore, a taking and placing groove which is convenient for taking out the slide is arranged on the card at the upper end of the empty groove.
Further, a folding dyeing frame, still including accomodating the frame, accomodate the frame including a frame, four sides and the equal interval in bottom of frame are provided with the pin, and the upper end of frame is provided with the handle.
The using method of the folding dyeing stand comprises the following steps:
(1) the glass slides are clamped in the empty grooves, the transverse ribs are used for supporting the glass slides, and the blocking piece groups in front of and behind the empty grooves are used for clamping the glass slides to prevent the glass slides from falling off from the empty grooves;
(2) the clamping plate is folded in a zigzag manner through the pulling plate and is vertically placed in the containing frame;
(3) then, washing the glass slide, and carrying out spin-drying by a handle;
(4) when the glass slide needs to be flattened, the clamping plates are taken out of the storage rack, and the plurality of clamping plates are flattened once through the pulling plate.
A horizontal incubation box comprises the folding staining rack.
Further, a box is hatched to level, still include the box frame and set up in the lid of box frame upper end, the four corners of box frame lower extreme all is provided with height-adjustable's pad foot, the central point at lid top puts and is provided with the level bubble, the both sides of box frame upper end all are provided with the draw-in groove with second connecting piece one-to-one, be provided with the space bar in the box frame between two draw-in grooves, the space bar is perpendicular with the bottom of box frame, the upper end and the box frame bottom and the lid top of space bar keep same horizontal position, the space bar has divided into a plurality of basins with the box frame, be provided with the intercommunicating pore of connecting adjacent basin on the space bar, the cardboard is placed on the space bar along the horizontal direction, the draw-in groove clamps with the.
The use method of the horizontal incubation box comprises the following steps:
1) observing the horizontal bubble of the box cover, and adjusting the heights of the foot pads at the four corners of the box frame to keep the box frame horizontal;
2) unfolding a plurality of clamping plates with slides through a pulling plate, wherein the clamping plates are horizontally arranged at the upper ends of the partition plates;
3) the rotating shaft of the second connecting piece is clamped in the corresponding clamping groove, the pulling plate is arranged on the outer side of the box frame, and finally the box cover is covered.
The invention has the beneficial effects that:
according to the invention, through the foldable staining rack, the quick switching of the horizontal incubation and vertical repair washing processes in the immunohistochemical experiment process is realized; through the incubation box matched with the folding dyeing frame, the visual operability of horizontal incubation is realized, and meanwhile, the support of the clamping plate, the clamping groove and the clamping of the second connecting piece rotating shaft are realized through the spacing plate, so that the placing and application problems of the folding dyeing frame are solved.
In the process from the selected section to the dyeing, the slide does not need to be taken down, and the operation is quick and convenient; the staining rack can save a large amount of operation time for a round of immunohistochemical experiments; in the operation process, the slide does not need to be taken down, the position of the slice is relatively fixed, and errors caused by the discharge problem can be avoided after the arrangement is well set; not worrying that the slide does not place, placing the dyeing frame that expandes on the incubation box that adjusts in advance, whether level can be quick affirmation, need not confirm one by one again, save time, the simple operation avoids appearing the slide that flattens earlier and dewaters the dry piece easily and produce non-characteristic, has improved the handling capacity of efficiency and single sample.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of a folding dyeing stand according to the present invention;
FIG. 2 is a cross-sectional view A-A of FIG. 1;
FIG. 3 is a folding dyer with right-angled baffles;
FIG. 4 is a schematic structural view of the storage rack;
FIG. 5 is a top view of the lid;
FIG. 6 is a top view of the cassette frame with the staining rack;
FIG. 7 is a top view of the box frame with the staining rack removed;
FIG. 8 is a schematic view of the structure of B-B in FIG. 7.
The glass slide packaging box comprises a clamping plate 1, a pulling plate 2, a first connecting piece 3, a second connecting piece 4, a glass slide 5, a transverse rib 6, a baffle 7, a magnetic sheet 8, a taking and placing groove 9, a frame 10, a stop lever 11, a handle 12, a box frame 13, a box cover 14, a pad foot 15, a horizontal bubble 16, a clamping groove 17, a partition plate 18, a water groove 19, a communication hole 20 and an empty groove 21.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
Example one
As shown in fig. 1-3, a folding dyeing rack, including a plurality of cardboard 1 and two arm-tie 2, cardboard 1 adopts engineering plastics preparation, and a plurality of cardboard 1 is arranged in between two arm-tie 2, and a plurality of cardboard 1 is zigzag folding, is provided with folding first connecting piece 3 between the adjacent cardboard 1, and arm-tie 2 passes through second connecting piece 4 collapsible with adjacent card and links to each other, and first connecting piece 3 and second connecting piece 4 are the working of plastics of the hinge form that has the pivot.
The size of arm-tie 2 is 80X 30mm, the size of cardboard 1 is 80X 30mm, cardboard 1's thickness is about 2mm, slide 5's size is 75X 25mm, be equipped with the dead slot 21 that is used for holding slide 5 on the cardboard 1, the rear end interval of dead slot 21 is fixed with two horizontal muscle 6 that are used for supporting slide 5, 25mm and 50mm department if horizontal muscle 6 is fixed in the dead slot 21 rear end, both ends all are fixed with the separation blade group that is used for blocking slide 5 around the dead slot 21, separation blade group includes two baffles 7, baffle 7 can be rectangular plate (2X 10mm), also can be the right angle swash plate, the right angle swash plate is favorable to the drainage, two baffles 7 are located the both sides of dead slot 21 lower extreme respectively, baffle 7 is the rubber slab.
Both sides of the upper end of the clamping plate 1 are fixed with magnetic sheets 8, the attraction of the adjacent clamping plates 1 is realized through the magnetic sheets 8, the stability of the folded clamping plate is ensured, the magnetism is not easy to be too large, and the requirement is easy to expand. The card at the upper end of the empty slot 21 is provided with a taking and placing slot 9 which is convenient for taking out the slide 5.
The application method of the embodiment comprises the following steps: the slide 5 is clamped in the empty slot 21, the transverse ribs 6 are used for supporting the slide 5, and the blocking piece groups at the front and the rear of the empty slot 21 are used for clamping the slide 5 so as to prevent the slide 5 from sliding off from the lower end of the empty slot 21; when the clamping plates 1 need to be folded, the clamping plates 1 can be folded towards the middle through the pulling plates 2, and the magnetic sheets 8 attract the adjacent clamping plates 1 to realize the folding stability; when the clamping plate 1 needs to be unfolded, the clamping plate 1 is pulled open towards two sides through the pulling plate 2 and is driven to be unfolded together.
Example two
This embodiment is substantially the same as the first embodiment, except that: as shown in figure 4, a folding dyeing frame, still including accomodating the frame, accomodating the frame and adopting the engineering plastics preparation, accomodating the frame including frame 10, four sides and the equal interval in bottom of frame 10 are fixed with and are used for blocking pin 11 of cardboard 1, and the upper end of frame 10 is fixed with handle 12, and the side that frame 10 upper end and handle 12 link to each other is higher than adjacent side, if exceed 3 centimetres, the business turn over of cardboard 1 of being convenient for, the washing of the arm-tie 2 of being convenient for of handle is spin-dried.
EXAMPLE III
The use method of the folding dyeing stand in the embodiment II comprises the following steps:
(1) the slide 5 is clamped in the empty slot 21, the transverse ribs 6 are used for supporting the slide 5, and the blocking piece groups at the front and the rear of the empty slot 21 are used for clamping the slide 5 so as to prevent the slide 5 from sliding off the empty slot 21;
(2) the clamping plate 1 is folded in a zigzag manner through the pulling plate 2 and is vertically placed in the containing frame;
(3) then, washing the glass slide 5, and carrying out spin-drying by a handle;
(4) when needing flat slide 5 of exhibition, take out cardboard 1 from accomodating the frame, once flatten a plurality of cardboard 1 through arm-tie 2.
Example four
As shown in fig. 5-8, a horizontal incubation box includes a folded staining rack as described in the first embodiment.
The utility model provides a box is hatched to level, still includes box frame 13 and clamps in box lid 14 of box frame 13 upper end, and the four corners of box frame 13 lower extreme all is fixed with height-adjustable pad foot 15, if the upper end of pad foot 15 links to each other with box frame 13 through adjusting screw, adjusts the height through rotatory adjusting screw, also can be other height-adjusting structure.
The central point at lid 14 top puts and installs horizontal bubble 16, and the both sides of box frame 13 upper end all are equipped with the draw-in groove 17 with second connecting piece 4 one-to-one, and the box frame 13 internal fixation between two draw-in grooves 17 has a space bar 18, and space bar 18 is perpendicular with the bottom of box frame 13, and the upper end of space bar 18 keeps parallel to each other with box frame 13 bottom and lid 14 top plane, and at same horizontal position, be favorable to guaranteeing that a plurality of cardboard 1 keeps same horizontal plane after expanding. The partition plate 18 divides the cartridge frame 13 into a plurality of water tanks 19, and the partition plate 18 is provided with communication holes 20 for connecting the adjacent water tanks 19 to maintain the water levels between the respective areas at the same height.
The clamping plate 1 is placed on the partition plate 18 along the horizontal direction, the clamping groove 17 is connected with the rotating shaft of the second connecting piece 4 in a clamping mode, and the pulling plate 2 is placed on the outer side of the box frame 13. If the pulling plate 2 and the adjacent cards are connected in a foldable manner through the two second connecting pieces 4, the box frame 13 is provided with at least one row of clamping plates 1 along the horizontal direction, the single row is 61 multiplied by 9 multiplied by 3cm in size, and the double row is 61 multiplied by 18 multiplied by 3cm in size, so that two sides of the upper end of the box frame 13 are provided with at least two clamping grooves 17.
EXAMPLE five
The use method of the horizontal incubation box comprises the following steps:
1) observing a horizontal bubble 16 of the box cover 14, and adjusting the heights of the pad feet 15 at four corners of the box frame 13 to keep the box frame 13 horizontal;
2) unfolding a plurality of clamping plates 1 with slides 5 through a pulling plate 2, wherein the clamping plates 1 are horizontally arranged at the upper ends of partition plates 18;
3) the rotating shaft of the second connecting piece 4 is clamped in the corresponding clamping groove 17, the pulling plate 2 is arranged on the outer side of the box frame 13, and finally the box cover 14 is covered.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A folding dyeing stand is characterized in that: including a plurality of cardboard (1), a plurality of cardboard (1) is zigzag folding, be provided with folding first connecting piece (3) between adjacent cardboard (1), be provided with dead slot (21) that are used for holding slide (5) on cardboard (1), the rear end interval of dead slot (21) is provided with horizontal muscle (6) that are used for supporting slide (5), both ends all are provided with the separation blade group that is used for blocking slide (5) around dead slot (21), the separation blade group includes two baffle (7), two baffle (7) are located the both sides of dead slot (21) lower extreme respectively.
2. A folding dyeing stand according to claim 1, characterized in that: still include two arm-tie (2), a plurality of cardboard (1) is arranged in between two arm-tie (2), and arm-tie (2) pass through second connecting piece (4) collapsible the linking to each other with adjacent card, and first connecting piece (3) and second connecting piece (4) are the hinge that has the pivot.
3. A folding dyeing stand according to claim 1 or 2, characterized in that: both sides of the upper end of the clamping plate (1) are provided with magnetic sheets (8), and the attraction of the adjacent clamping plates (1) is realized through the magnetic sheets (8).
4. A folding dyeing stand according to claim 1, characterized in that: the card at the upper end of the empty groove (21) is provided with a taking and placing groove (9) which is convenient for taking out the slide (5).
5. A folding dyeing stand according to claim 1, characterized in that: the storage rack comprises a rack frame (10), stop rods (11) are arranged at the four sides and the bottom of the rack frame (10) at intervals, and a handle (12) is arranged at the upper end of the rack frame (10).
6. Use of a folding dyeing stand according to one of claims 1 to 5, characterized in that it comprises the following steps:
(1) the glass slide (5) is clamped in the empty groove (21), the transverse ribs (6) are used for supporting the glass slide (5), and the blocking piece groups at the front and the rear of the empty groove (21) are used for clamping the glass slide (5) to prevent the glass slide (5) from sliding off the empty groove (21);
(2) the clamping plate (1) is folded in a zigzag manner through the pulling plate (2) and is vertically placed in the containing frame;
(3) then, washing the glass slide (5), and carrying out spin-drying by a handle;
(4) when the glass slide (5) needs to be flattened, the clamping plates (1) are taken out of the storage rack, and the clamping plates (1) are flattened once through the pulling plates (2).
7. A horizontal incubation cassette comprising a folded staining rack according to any one of claims 1 to 4.
8. The horizontal incubation box according to claim 7, further comprising a box frame (13) and a box cover (14) arranged at the upper end of the box frame (13), wherein four corners of the lower end of the box frame (13) are provided with height-adjustable foot pads (15), a horizontal bubble (16) is arranged at the center of the top of the box cover (14), two sides of the upper end of the box frame (13) are provided with clamping grooves (17) corresponding to the second connecting pieces (4) one by one, a vertical spacing plate (18) is arranged in the box frame (13) between the two clamping grooves (17), the box frame (13) is divided into a plurality of water grooves (19) by the spacing plate (18), communication holes (20) for connecting the adjacent water grooves (19) are arranged on the spacing plate (18), the clamping plate (1) is placed on the spacing plate (18) along the horizontal direction, the clamping grooves (17) are connected with the rotating shaft clamp of the second connecting pieces (4), and the pulling plate (2) is placed at the outer side of the.
9. The use of a horizontal incubation cartridge of claim 7 or 8, comprising the steps of:
1) observing a horizontal bubble (16) of the box cover (14), and adjusting the heights of the pad feet (15) at four corners of the box frame (13) to keep the box frame (13) horizontal;
2) unfolding a plurality of clamping plates (1) with slides (5) through a pulling plate (2), wherein the clamping plates (1) are horizontally arranged at the upper ends of partition plates (18);
3) the rotating shaft of the second connecting piece (4) is clamped in the corresponding clamping groove (17), the pulling plate (2) is arranged on the outer side of the box frame (13), and finally the box cover (14) is covered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010235598.8A CN111521470A (en) | 2020-03-30 | 2020-03-30 | Folding dyeing rack, horizontal incubation box and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010235598.8A CN111521470A (en) | 2020-03-30 | 2020-03-30 | Folding dyeing rack, horizontal incubation box and using method |
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| Publication Number | Publication Date |
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| CN111521470A true CN111521470A (en) | 2020-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010235598.8A Withdrawn CN111521470A (en) | 2020-03-30 | 2020-03-30 | Folding dyeing rack, horizontal incubation box and using method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092220A (en) * | 2021-04-07 | 2021-07-09 | 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） | Immunohistochemical wet box |
-
2020
- 2020-03-30 CN CN202010235598.8A patent/CN111521470A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092220A (en) * | 2021-04-07 | 2021-07-09 | 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） | Immunohistochemical wet box |
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Application publication date: 20200811 |