CN211791318U

CN211791318U - Charging and discharging circuit for electrotransformation instrument

Info

Publication number: CN211791318U
Application number: CN201921109358.2U
Authority: CN
Inventors: 邹翠云; 江福能; 张鑫; 张蓓; 阴晓丽; 钟惟德; 朱建国
Original assignee: Guangzhou Jiaolong Cell Pharmaceutical Technology Co ltd
Current assignee: Guangzhou Boyao Biotechnology Co ltd
Priority date: 2019-07-15
Filing date: 2019-07-15
Publication date: 2020-10-27
Anticipated expiration: 2029-07-15

Abstract

The utility model relates to a charge-discharge circuit for electrotransformation appearance, include: the charging circuit, the charging capacitor, the discharging circuit and the discharging circuit; the charging capacitor is respectively connected with the charging circuit, the discharging circuit and the discharging circuit; the charging circuit is connected to an external power supply to charge the charging capacitor; the discharge circuit discharges the charged capacitor to output the required pulse; and the electricity discharge circuit releases the residual electric energy of the charging capacitor after the discharging process is finished so as to ensure the safety.

Description

Charging and discharging circuit for electrotransformation instrument

Technical Field

The utility model belongs to the technical field of biomedical equipment technique and specifically relates to a charge-discharge circuit for electrotransformation appearance is related to.

Background

Transfection refers to the introduction of a gene of interest into a eukaryotic cell by biochemical or physical means. Gene transfection, the transfer of specific genetic information into integrated cells, has not only revolutionized the research of many fundamental problems in biology and medicine, but has also driven the development of molecular techniques in diagnosis and therapy, and made gene therapy possible. At present, gene transfection is widely applied to the research of gene structure and function analysis, gene expression and regulation, gene therapy, transgenic animals and the like.

Cell or bacterial electrotransfection is a technique that uses electrical impulses to introduce into cells macromolecules that cannot penetrate the cell membrane. Upon application of a strong electric field, the cell membrane temporarily becomes porous and permeable to foreign materials. Cell membrane electroporation depends on various parameters of the electric field, such as pulse type, pulse voltage, pulse duration, number of pulses, and other experimental conditions. At present, the devices for cell electrotransfection mainly comprise a cell electrotransformation instrument, an electrode cup and the like.

Utility model people discover in studying that the circuit of electrotransformation appearance will realize accurate pulse parameter control to guarantee the security, its cost is than higher. With the generalization of electrode cups and the popularization of electrotransformation experiments in research laboratories, it is necessary to provide a low-cost charging and discharging circuit which can ensure safety and release pulses required by experimental research.

SUMMERY OF THE UTILITY MODEL

In view of the above, there is a need to provide a charge and discharge circuit for an electrical transfer apparatus, which has the advantages of low cost, capability of realizing pulses required by experimental research, and safety.

A charging and discharging circuit for an electrotransformation machine, comprising: the charging circuit, the charging capacitor, the discharging circuit and the discharging circuit; the charging capacitor is respectively connected with the charging circuit, the discharging circuit and the discharging circuit; the charging circuit is connected to an external power supply to charge the charging capacitor; the discharge circuit discharges the charged capacitor to output the required pulse; and the electricity discharge circuit releases the residual electric energy of the charging capacitor after the discharging process is finished so as to ensure the safety.

The charging circuit comprises a charging switch, a first optocoupler and a charging IGBT driven by the first optocoupler; the charging circuit is used for charging the charging capacitor when the charging switch is turned on and the charging IGBT is switched on.

The charging switch is a charging relay.

The discharging circuit comprises a discharging switch, a second optocoupler and a discharging IGBT driven by the second optocoupler; the discharging circuit discharges the charging capacitor when the discharging switch is turned on and the discharging IGBT is turned on.

The discharge switch is a discharge relay.

The discharging circuit further comprises a discharging terminal connected with the discharging IGBT and a discharging resistor connected with the discharging terminal in parallel; the discharge terminals are used for externally connecting two poles of the electric revolving cup.

The discharge line further comprises a fuse tube connected in series with the discharge terminal.

The electricity leakage circuit comprises a third optocoupler and an electricity leakage IGBT driven by the third optocoupler; and when the electricity leakage IGBT is switched on, the electricity leakage circuit releases the electric energy of the charging capacitor.

The utility model provides a charge-discharge circuit for electrotransformation appearance, including charging capacitor, charging circuit, discharge line and discharge line, it has low-cost advantage, can realize the required pulse of experimental study to guarantee the security.

Drawings

Fig. 1 is a schematic structural diagram of a charge and discharge circuit for an electrotransformation machine provided by the present invention;

fig. 2 is a schematic circuit diagram of a charging and discharging circuit for an electrical transfer apparatus according to an embodiment of the present invention.

Detailed Description

Referring to fig. 1, in one embodiment of the present invention, a charging and discharging circuit for an electrotransformation machine is provided. In this embodiment, a charge and discharge circuit for an electrotransformation machine, comprising: the charging circuit, the charging capacitor, the discharging circuit and the discharging circuit; the charging capacitor is respectively connected with the charging circuit, the discharging circuit and the discharging circuit; the charging circuit is connected with an external power supply to charge the charging capacitor; a discharge circuit for discharging the charged capacitor to output the required pulse; and the electricity discharge circuit releases the residual electric energy of the charging capacitor after the discharging process is finished so as to ensure the safety.

Referring to fig. 2, a charging and discharging circuit for an electrical transfer apparatus according to another embodiment of the present invention is provided. Specifically, in fig. 2, reference numeral 1 denotes a charging relay. Reference numeral 2 denotes a charging IGBT, preferably a high-speed high-voltage IGBT, which charges a charging capacitor. Further, the charging IGBT in fig. 2 is driven by the opto-coupler 21. And 3, a charging capacitor, in particular a high-voltage large-capacity capacitor. And 4, a power-off IGBT which controls power-off action and is driven by the optocoupler 41. And 5 is a discharge relay. And 6 is a discharge terminal which is externally connected with two poles of an electric rotating cup, and the electric rotating cup can be a conventional electric rotating cup which is filled with liquid for an electric rotating experiment. 7 is a discharging IGBT, and correspondingly, 71 is an optocoupler for driving the discharging IGBT. And 10 is a discharge resistor.

Next, the operation principle of the charge and discharge circuit for the electrotransport device in the embodiment of fig. 2 will be explained. Briefly, the duty cycle is the charge-discharge process.

Specifically, the charging relay 1 is turned on, the charging IGBT (driven by the corresponding optocoupler) is turned on, the parts 4, 5 and 7 are turned off, and the charging process is started. Typically, the charge capacitance time is about 25 s. Optionally, the charging time may be adjusted according to the high-voltage power supply power and the charging resistor, the circuit includes a voltage feedback to detect the capacitor voltage, and when the voltage reaches a set voltage, the charging is stopped.

When the charging process is finished, the devices 1, 2, 4 are closed, the devices 5, 7 are opened, and the charging capacitor is in the discharging stage. During discharging, the voltage loaded on the discharging terminal decays exponentially.

The discharging time is controllable and adjustable, after the discharging process is finished, the component 4 is opened, the components 1, 2, 5 and 7 are closed, the discharging process is started, the residual electric energy on the charging capacitor is released, the charging capacitor is ensured not to have points after the working cycle is finished, the damage is avoided, and the safety of the next working cycle is also ensured.

The charge and discharge circuit for the electrotransformation instrument provided by the embodiment of fig. 2 has the following advantages:

(1) the circuit is simple and reliable, the structure is clear, the charging circuit, the discharging circuit and the discharging circuit are included, only 3 IGBTs are used for expensive devices in the circuit, and the cost is low.

(2) The pulse waveform required by experimental research can be realized. During discharging, theoretical discharging voltage shows exponential decay, and discharging time is controllable, so that square wave discharging can be approximately obtained by reasonably controlling the discharging time, for example, about 20 microseconds per time. Further, any required pulse waveform can be obtained by superposing square waves with different periods.

(3) The discharge time is short, and the energy is large, and is generally within 1.5 milliseconds.

(4) Realize the heavy current and discharge, can be suitable for the cell or bacterium electricity in laboratory and change the scene.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A charge-discharge circuit for an electrotransport device, comprising: the charging circuit, the charging capacitor, the discharging circuit and the discharging circuit; the charging capacitor is respectively connected with the charging circuit, the discharging circuit and the discharging circuit; the charging circuit is connected to an external power supply to charge the charging capacitor; the discharge circuit discharges the charged capacitor to output the required pulse; and the electricity discharge circuit releases the residual electric energy of the charging capacitor after the discharging process is finished so as to ensure the safety.

2. The charging and discharging circuit for an electric transducer according to claim 1, wherein the charging circuit comprises a charging switch, a first optical coupler and a charging IGBT driven by the first optical coupler; the charging circuit is used for charging the charging capacitor when the charging switch is turned on and the charging IGBT is switched on.

3. The charging and discharging circuit for an electrotransformation machine of claim 2, wherein the charging switch is a charging relay.

4. The charge-discharge circuit for an electrotransformation machine of claim 1, wherein the discharge line comprises a discharge switch, a second optocoupler, and a discharge IGBT driven by the second optocoupler; the discharging circuit discharges the charging capacitor when the discharging switch is turned on and the discharging IGBT is turned on.

5. The charging and discharging circuit for an electrotransformation machine of claim 4, wherein the discharge switch is a discharge relay.

6. The charge-discharge circuit for an electrotransport device of claim 4 wherein the discharge line further comprises a discharge terminal connected to the discharge IGBT and a discharge resistor in parallel with the discharge terminal; the discharge terminals are used for externally connecting two poles of the electric revolving cup.

7. The charge-discharge circuit for an electrotransport device of claim 6 wherein the discharge line further comprises a fuse in series with the discharge terminal.

8. The charging and discharging circuit for an electrotransformation machine of claim 1, wherein the discharging circuit comprises a third optocoupler and a discharging IGBT driven by the third optocoupler; and when the electricity leakage IGBT is switched on, the electricity leakage circuit releases the electric energy of the charging capacitor.