CN211771356U

CN211771356U - Cell culture device for stem cell detection

Info

Publication number: CN211771356U
Application number: CN201922207904.2U
Authority: CN
Inventors: 不公告发明人
Original assignee: Beijing Taisheng Kangyuan Biomedical Research Institute Co Ltd
Current assignee: Beijing Taisheng Kangyuan Biomedical Research Institute Co Ltd
Priority date: 2019-12-11
Filing date: 2019-12-11
Publication date: 2020-10-27
Anticipated expiration: 2029-12-11

Abstract

The utility model discloses a cell culture device for stem cell detection, which comprises a shell, wherein the left side inside the shell is a water sump, the top of the water sump is provided with a water inlet, the bottom of the water sump is provided with a water dropping device, the water tank is internally provided with a water bottle, the bottom of the water bottle is sleeved in the water dropping device, the water dropping device comprises a flow guide pipe, a ball and an elastic membrane, the diameter of the inner part of the flow guide pipe is gradually reduced, the ball is sleeved in the flow guide pipe, the elastic membrane is assembled in the flow guide pipe, the surface of the elastic membrane is provided with an opening, the top of a lower heating bin is a culture bin, a culture dish is arranged inside the culture bin, the ball is matched with the elastic membrane, the surface tension of water is matched with the membrane elasticity and the gravity of the ball, the slow dripping of water flow is realized, the flow velocity is controlled, the upper and lower heating plates provide the temperature, the device is more sanitary, the degerming effect is stronger, and the culture result is more accurate.

Description

Cell culture device for stem cell detection

Technical Field

The utility model relates to the technical field of medical equipment, specifically be a cell culture device is used in stem cell detection.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an indispensable process for both the whole biological engineering technology and one of the biological cloning technologies, the cell culture is a large-scale clone of cells, and in the actual culture process, the condition that the culture dish is polluted to cause inaccurate results is often caused because the sterile environment of the device cannot be completely formed.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a stem cell detects uses cell culture device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a cell culture device for stem cell detection comprises a shell, wherein a water bin is partitioned on the left side inside the shell, a water inlet is formed in the top of the water bin, a water bin cover is hinged to the left side wall of the water inlet, a clamping frame is assembled on the rear side wall of the water bin, a water dropping device is assembled at the bottom of the water bin, a water bottle is sleeved inside the clamping frame, the bottom of the water bottle is sleeved in the water dropping device, the water dropping device comprises a flow guide pipe, balls and an elastic membrane, the bottom of the flow guide pipe is bent rightwards to enter a lower heating bin, the diameter of the inside of the flow guide pipe is gradually reduced, the balls are sleeved in the flow guide pipe, the elastic membrane is assembled in the flow guide pipe, a linear opening is formed in the surface of the elastic membrane, a culture bin is formed in the top of the lower heating bin, a culture bin opening is formed in the right side wall of the culture, and the top of the culture bin is an upper heating bin, and the top of the upper heating bin is a ventilation bin.

Preferably, the upper heating chamber and the lower heating chamber are respectively provided with an electric heating plate, and the electric heating plates are connected with an external power supply.

Preferably, the right side wall of the ventilation bin is provided with an air hole, and the inside of the ventilation bin is filled with activated carbon.

Preferably, the top wall of the left side of the lower heating bin, the top wall of the right side of the culture bin and the top wall of the left side of the upper heating bin are all provided with ventilation holes.

Preferably, gauze is arranged in the air hole and the ventilation hole in the top wall of the left side of the upper heating bin.

Preferably, the front surface of the casing is provided with a window at a position corresponding to the water bottle and the culture dish.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses set up the device that drips that ball and elastic film cooperation were used, utilized the surface tension of water and the cooperation of membrane elasticity and ball gravity, realized the slow drippage of rivers, the control flow rate, the upper and lower hot plate provides the required temperature of cell culture simultaneously, heated air and water, avoided cold air direct contact culture dish, and be the vapor with the water distillation, avoid polluting the culture dish, active carbon adsorbs impurity, it is more sanitary to make the device, the degerming effect is stronger, the culture result is more accurate.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an appearance schematic diagram of the present invention.

Fig. 3 is an enlarged sectional view of the draft tube.

In the figure: 1. the device comprises a shell, 11, a water inlet, 12, a dish opening, 13, an electric heating plate, 14, ventilation holes, 15, gauze, 16, a window, 2, a water bin, 21, a water bin cover, 22, a clamping frame, 23, a water bottle, 3, a water dripping device, 31, a guide pipe, 32, balls, 33, an elastic membrane 4, a lower heating bin, 5, a culture bin, 51, a culture bin cover, 52, a culture dish, 6, an upper heating bin, 7, a ventilation bin, 71, an air hole, 72 and active carbon.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1, the present invention provides a technical solution: a cell culture device for stem cell detection comprises a shell 1, a water bin 2 is arranged on the left side inside the shell 1, a water inlet 11 is formed in the top of the water bin 2, a water bin cover 21 is hinged to the left side wall of the water inlet 11, a clamping frame 22 is assembled on the rear side wall of the water bin 2, a water dripping device 3 is assembled at the bottom of the water bin 2, a water bottle 23 is sleeved inside the clamping frame 22, the bottom of the water bottle 23 is sleeved in the water dripping device 3, the water dripping device 3 comprises a flow guide pipe 31, a ball 32 and an elastic membrane 33, the bottom of the flow guide pipe 31 is bent rightwards to enter a lower heating chamber 4, the diameter inside the flow guide pipe 31 is gradually reduced, the ball 32 is sleeved in the flow guide pipe 31, the elastic membrane 33 is assembled in the flow guide pipe 31, an opening shaped like a Chinese character 'yi' is marked on the surface of the elastic membrane 33, the water bin cover 21 is lifted, the lower portion below the water bottle 23, because of the surface tension of water and the elastic force of the elastic membrane 33, the water will not drop, the liquid level between the elastic membrane 33 and the ball 32 rises as the amount of water seeps more and more, when the water rises to the position of the ball 32, produce ascending thrust to ball 32, roll on ball 32, water flows down rapidly in the honeycomb duct 31 this moment, elasticity film 33 elasticity is less than the gravity of water, water drips from elasticity film 33 surface cut-out department, liquid level descends between elasticity film 33 and the ball 32 this moment, ball 32 rolls down, and is repeated, water lasts the drippage, get into lower heated warehouses 4, lower heated warehouses 4 top is for cultivateing storehouse 5, lower heated warehouses 4 toast vapor into and cultivate storehouse 5, it has dish mouth 12 to cultivate 5 right side walls in storehouse, dish mouth 12 roof articulates there is cultivation storehouse lid 51, cultivate 5 inside culture dish 52 of having placed in storehouse, and cultivate 5 tops in storehouse and be last heated warehouses 6, it is ventilation chamber 7 to go up heated warehouses 6 tops.

Specifically, the upper heating chamber 6 and the lower heating chamber 4 are respectively provided with the electric heating plates 13, the electric heating plates 13 are connected with an external power supply, the power supply is switched on, the electric heating plates 13 start to heat, the temperature required by cell culture is provided, water drops are baked into water vapor and provided for cells, and the phenomenon that the culture dish is polluted by bacteria, dust and the like in water due to the fact that water directly contacts the culture dish is avoided.

Specifically, the air hole 71 is opened on the right side wall of the ventilation bin 7, and the activated carbon 72 is filled in the ventilation bin 7, so that the activated carbon 72 can adsorb dust and impurities in the air, and prevent the culture dish from being polluted.

Specifically, the upper heating chamber 4 left side roof, cultivate 5 right side roofs of storehouse, the upper heating chamber 6 left side roof has all opened the draught hole 14, and lower heating chamber 4 left side roof draught hole 14 can make vapor get into and cultivate storehouse 5, and the draught hole 14 of cultivating 5 right side roofs and the upper heating chamber 6 left side roof makes the air be "it" style of calligraphy entering cultivation storehouse, makes the air pass active carbon 72 earlier and passes through electric plate 13 surface again, avoids cold air direct contact culture dish 52.

Specifically, gauze 15 is arranged in the air hole 71 and the ventilation hole 14 on the left top wall of the upper heating chamber 6, so as to prevent the active carbon 72 from falling out of the ventilation chamber 7.

Specifically, the front surface of the housing 1 is provided with a window 16 at a position corresponding to the water bottle 23 and the culture dish 52, so that the cell culture condition can be observed conveniently and the water can be added in time.

The working principle is as follows: lifting the water bin cover 21, inserting the lower part of the water bottle 23 into the top of the flow guide pipe 31, leading the water to flow in the flow guide pipe 31, slowly seeping from the balls 32 and dropping on the elastic membrane 33, wherein the water cannot drop due to the surface tension of the water and the elastic force of the elastic membrane 33, the liquid level between the elastic membrane 33 and the balls 32 rises as the seeped water amount increases, when the water rises to the position of the balls 32, upward thrust is generated on the balls 32, the water in the flow guide pipe 31 rapidly flows down, the elastic force of the elastic membrane 33 is smaller than the gravity of the water, the water drops from the cut on the surface of the elastic membrane 33, the liquid level between the elastic membrane 33 and the balls 32 drops, the balls 32 roll down, the circulation is repeated, the water continuously drops and enters the lower heating bin 4, the power supply is switched on, the electric heating plate 13 starts to heat, the required temperature for cell culture is provided, the lower heating bin 4 bakes the water into water vapor and then enters, avoid water direct contact culture dish and lead to aquatic bacterium dust etc. to cause the pollution to the culture dish, the required air of cell breathing and produce waste gas and exchange with the external world through gas pocket 71 and scavenge port 14, at this in-process, the air is "the" style of calligraphy and passes active carbon 72 earlier and get into culture bin 5 through electric plate 13 surface again, dust and impurity in the adsorbed air avoid cold air direct contact culture dish 52, prevent to pollute the culture dish.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A cell culture device for stem cell detection, comprising a housing (1), characterized in that: the utility model discloses a water-saving and water-saving device, including shell (1), sump (2), water inlet (11) left side wall are articulated to have sump lid (21), sump (2) rear side wall is equipped with card frame (22), and sump (2) bottom is equipped with water dripping device (3), water-jug (23) have been cup jointed to card frame (22) inside, water-jug (23) bottom cup joints in water dripping device (3), water dripping device (3) include honeycomb duct (31), ball (32) and elastic film (33), honeycomb duct (31) bottom is buckled right and is got into down heating chamber (4), honeycomb duct (31) inside diameter reduces gradually, ball (32) cup joint in honeycomb duct (31), elastic film (33) assembles in honeycomb duct (31), and elastic film (33) surface has drawn "one" style of calligraphy opening, lower heated warehouses (4) top is for cultivateing storehouse (5), it has dish mouth (12) to cultivate storehouse (5) right side wall open, dish mouth (12) roof articulates there is culture storehouse lid (51), culture dish (52) have been placed to culture storehouse (5) inside, and culture storehouse (5) top is last heated warehouses (6), it is storehouse (7) of ventilating to go up heated warehouses (6) top.

2. The cell culture apparatus for stem cell detection according to claim 1, characterized in that: the upper heating chamber (6) and the lower heating chamber (4) are respectively provided with an electric heating plate (13), and the electric heating plates (13) are connected with an external power supply.

3. The cell culture apparatus for stem cell detection according to claim 1, characterized in that: the right side wall of the air exchange bin (7) is provided with an air hole (71), and the inside of the air exchange bin (7) is filled with activated carbon (72).

4. The cell culture apparatus for stem cell detection according to claim 1, characterized in that: the upper heating chamber (6) is provided with a plurality of ventilation holes (14) respectively.

5. The cell culture apparatus for stem cell detection according to claim 3, characterized in that: gauze (15) are arranged in the air hole (71) and the ventilation hole (14) on the left top wall of the upper heating bin (6).

6. The cell culture apparatus for stem cell detection according to claim 1, characterized in that: a window (16) is arranged on the front surface of the shell (1) corresponding to the water bottle (23) and the culture dish (52).