CN211771226U - A fixing device for cell freeze thawing process - Google Patents
A fixing device for cell freeze thawing process Download PDFInfo
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- CN211771226U CN211771226U CN201922402858.1U CN201922402858U CN211771226U CN 211771226 U CN211771226 U CN 211771226U CN 201922402858 U CN201922402858 U CN 201922402858U CN 211771226 U CN211771226 U CN 211771226U
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Abstract
The utility model relates to a fixing device for cell freeze thawing process, the device includes: the freezing and thawing box comprises a freezing and thawing box body, a freezing and thawing tube placing frame and a freezing and thawing box body, wherein the freezing and thawing box body is detachably connected with the side wall of the freezing and thawing box body and is arranged inside the freezing and thawing box body; the clamping grooves are arranged in the arc-shaped grooves, a plurality of clamping grooves are arranged, each clamping groove is provided with an inserting piece with a corresponding size in a matched mode, and the interval between every two adjacent clamping grooves in each arc-shaped groove is matched with the height of the cryopreservation pipe placed in the arc-shaped groove; and the upper cover is covered on the top opening of the freezing and thawing box. The utility model has the advantages that: placing the freezing tube for containing the cells horizontally for cell freeze thawing to improve the titer of the cells after freeze thawing; meanwhile, the heating device is arranged at the bottom of the freezing storage tube placing groove, so that the operation of taking the freezing storage tube out to the room temperature environment can be omitted, and the operation steps are simplified; in addition cell freeze thawing fixing device can also place different specifications simultaneously and freeze and deposit the pipe and carry out the cell freeze thawing.
Description
Technical Field
The utility model relates to a cell thawing technical field particularly, relates to a fixing device for cell freezing and thawing process.
Background
The cell freeze thawing means that the cells to be crushed are cooled to-15 ℃ to-20 ℃, then are rapidly thawed at room temperature or 40 ℃, and the cell freeze thawing is repeated for many times, so that the cells are swelled and crushed due to the fact that the salt concentration of the residual cell sap is increased because of ice grains formed in the cells. In the existing freeze-thawing process, the freezing tube is basically vertically inserted into the freezing box and placed in a liquid nitrogen tank for freezing, after freezing is finished, the freezing tube is taken out from the liquid nitrogen tank and placed in a room temperature environment, and the whole freeze-thawing process of cells is finished by repeating the steps. On one hand, the operation steps of putting the freezing tube back and forth from the liquid nitrogen tank and taking out the freezing tube are complex, and on the other hand, the titer of the freezing tube after being vertically placed for cell freeze thawing is obviously lower than that of the freezing tube after being horizontally placed for cell freeze thawing.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problems, an object of the present invention is to provide a fixing device for a cell freezing and thawing process, which can facilitate the operation of the whole freezing and thawing process and improve the titer of the cells after freezing and thawing.
The utility model provides a fixing device for cell freeze thawing process, the device includes:
the freezing and thawing box comprises a freezing and thawing box body, a freezing and thawing tube placing frame and a freezing and thawing box body, wherein the freezing and thawing box body is detachably connected with the side wall of the freezing and thawing box body and is arranged inside the freezing and thawing box body;
the clamping grooves are arranged in the arc-shaped grooves, a plurality of clamping grooves are arranged, each clamping groove is provided with an inserting piece with a corresponding size in a matched mode, and the interval between every two adjacent clamping grooves in each arc-shaped groove is matched with the height of the cryopreservation pipe placed in the arc-shaped groove;
and the upper cover is covered on the top opening of the freezing and thawing box.
As a further improvement of the utility model, the outside of cryopreserving pipe rack is equipped with heating device.
As a further improvement, the freezing and storing pipe rack is connected with the side wall of the freezing and thawing box in a detachable manner through the connecting parts arranged at the two ends.
As a further improvement of the utility model, the cryopreserved pipe rack between two adjacent draw-in grooves be equipped with the parallel locking device of draw-in groove, locking device with two lateral walls of cryopreserved pipe rack can dismantle the connection.
As a further improvement of the utility model, the top outside of the upper cover is provided with a handle.
As a further improvement of the utility model, a sealing washer is arranged on the contact surface of the upper cover and the top opening of the freezing and thawing box.
As a further improvement, the freezing and thawing box side wall below the freezing and thawing tube placing rack is provided with a through hole and is communicated with the liquid nitrogen tank through a guide tube.
As a further improvement of the utility model, the heating device is a water bath heating device or an electric heating plate.
As a further improvement of the utility model, locking device is the clamp, the one end of clamp with it is articulated to freeze a lateral wall of depositing a tub rack, the other end of clamp with freeze another lateral wall bolted connection of depositing a tub rack.
As a further improvement of the utility model, be equipped with anti-skidding buffer gasket on the contact surface of locking device and cryopreserving pipe.
The utility model has the advantages that: placing the freezing tube for containing the cells horizontally for cell freeze thawing to improve the titer of the cells after freeze thawing; meanwhile, the heating device is arranged at the bottom of the freezing storage tube placing groove, so that the operation of taking the freezing storage tube out to the room temperature environment can be omitted, and the operation steps are simplified; in addition cell freeze thawing fixing device can also place different specifications simultaneously and freeze and deposit the pipe and carry out the cell freeze thawing.
Drawings
Fig. 1 is a top view of a fixing device for a cell freezing and thawing process according to an embodiment of the present invention;
fig. 2 is a front view of a fixing device for a cell freezing and thawing process according to an embodiment of the present invention;
fig. 3 is a left side view of a cryopreserving tube placing rack in a fixing device for a cell freezing and thawing process according to an embodiment of the present invention.
In the figure, the position of the upper end of the main shaft,
1. freezing and thawing box; 2. freezing and storing the tube rack; 3. a card slot; 4. an upper cover; 5. a handle; 6. an arc-shaped groove; 7. a connecting portion.
Detailed Description
The present invention will be described in further detail below with reference to specific embodiments and with reference to the attached drawings.
As shown in fig. 1-3, an embodiment of the present invention is a fixing device for a cell freezing and thawing process, the device comprising:
the freezing and storing pipe placing frame 2 is detachably connected with the side wall of the freezing and thawing box 1 and is arranged inside the freezing and thawing box 1, and the top of the freezing and storing pipe placing frame 2 is provided with an arc-shaped groove 6 matched with the radian of a freezing and storing pipe.
Because the titer after placing freezing tube horizontally and carrying out cell freeze thawing is obviously higher than the titer that freezing tube vertically placed and carry out cell freeze thawing, this application has set up arc recess 6 at the top of freezing tube rack 2, both can lie freezing tube in arc recess 6 like this to realize freezing tube lie and set up and be fixed in freeze-thaw case 1 and carry out cell freeze thawing. Will freeze and deposit a tub rack 2 and freeze thawing case 1 and set up and dismantle the connection, can be convenient for like this with freeze and deposit tub rack 2 and take out from freeze thawing case 1 and carry out operations such as disinfection, also be convenient for simultaneously from getting in the arc recess 6 and put the freeze and deposit the pipe. The operator does not need to directly stretch hands into the freezing and thawing box 1, so that the risk that the hands of the operator are frostbitten by liquid nitrogen is reduced.
The clamping grooves 3 are arranged in the arc-shaped grooves 6, the clamping grooves 3 are provided with a plurality of clamping pieces, each clamping groove 3 is provided with an inserting piece corresponding to the size in a matched mode, and the interval between every two adjacent clamping grooves 3 in each arc-shaped groove 6 is matched with the height of the freezing storage pipe placed in the arc-shaped groove.
After putting in arc recess 6 with freezing and depositing the pipe tie and lying, need stop fixedly to freezing both ends about depositing the pipe, consequently highly setting up the interval between two adjacent draw-in grooves 3 according to the freezing pipe of depositing placed in arc recess 6, later insert the inserted sheet that the draw-in groove 3 corresponds and insert draw-in groove 3, can be to freezing the fixed effect about depositing the pipe realization. In practical use, in order to facilitate the taking and placing of the freezing storage pipe, the effect of fixing the freezing storage pipe is achieved, and therefore the interval between every two adjacent clamping grooves 3 is larger than the height of the corresponding freezing storage pipe by 1-2 mm. Of course, in order to achieve the effect of fixing the freezing storage pipe by the inserting pieces, the height of the inserting pieces should be set to be 1-2 radiuses of the freezing storage pipe. The specifications of the conventional freezing tubes are mostly 0.5ml, 1.2ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml and the like, and the freezing tubes of the specifications have basically the same diameter and size and are different in height and size. Therefore, in order to facilitate cell cryopreservation on of cryopreservation tubes of various specifications, the lengths of two adjacent clamping grooves 3 in the same arc-shaped groove 6 can be set to be the lengths corresponding to the heights of the cryopreservation tubes of different specifications in actual use; also can set up the interval of two adjacent draw-in grooves 3 in same arc recess 6 to the same to correspondingly place a plurality of cryopreserved pipes rack 2, make each cryopreserved pipe rack 2 on the interval of draw-in groove 3 correspond the height that different specifications cryopreserved pipes. The specific setting mode can be determined according to specific requirements.
And the upper cover 4 is covered on the top opening of the freezing and thawing box 1. The upper cover 4 can seal the freezing and thawing box 1, so that the freezing and thawing box 1 achieves the effects of heat preservation or cold insulation, and meanwhile, the freezing and thawing box 1 can be isolated from the external environment, so that the cell freezing and thawing process is safer and more sanitary, and the leakage of liquid nitrogen is further avoided.
Further, the outside of the freezing storage tube placing frame 2 is provided with a heating device.
Further, the heating device is a water bath heating device or an electric heating plate.
In order to save the cell freeze thawing in-process with the frequent operation step of taking out among the room temperature environment of placing from freeze thawing case 1 of freezing and thawing pipe, this application has set up heating device in the outside of freezing and thawing pipe rack 2, can directly use heating device to carry out heat treatment in the freezing and thawing pipe when realizing the thawing recovery to the cell among the cell freeze thawing process, make the cell thawing recovery in the freezing and thawing pipe. In actual use, a corresponding control device needs to be arranged on the heating device, so that the switching function of the heating device and the temperature control function of the heating device are realized. The heating device should be arranged in the length direction of the outside of the whole freezing storage tube placing frame 2 in a penetrating way. The heating device in this embodiment selects and uses the electric hot plate for heating, and the heating plate cladding is in the outside of whole freezing pipe rack 2 to be connected the electric hot plate with the external control device electricity of freeze thawing case 1 through the wire. When the cells need to be thawed and revived, the control device is started to heat the electric heating plate, and the control device is operated to realize the temperature regulation and control of the electric heating plate. In the practical application process, a suitable heating device, such as a water bath heating device and the like, can be selected according to the type of the thawed cells.
Further, the freezing and storing tube placing frame 2 is detachably connected with the side wall of the freezing and thawing box 1 through the connecting parts 7 arranged at the two ends. Connecting portion 7 is for setting up in freezing the boss on depositing pipe rack 2 left and right ends bottom surface in this embodiment, is provided with the screw hole on the boss, also is provided with corresponding bolt hole simultaneously on the lateral wall of freeze thawing case 1, passes through bolted connection between the lateral wall of boss and freeze thawing case 1 to realize freezing the pipe rack 2 and freeze thawing case 1 between can dismantle and be connected. In the concrete connection process, a sealing ring can be arranged between the bolt and the threaded hole, so that the freezing and thawing box 1 has a better sealing effect. In practical application, other detachable connection modes can be selected, so long as the detachable connection between the cryopreservation tube placing frame 2 and the freezing and thawing box 1 can be realized, and the freezing and thawing effect of cells is not influenced.
Further, the freezing tube placing frame 2 between two adjacent clamping grooves 3 is provided with a locking device parallel to the clamping grooves 3, and the locking device is detachably connected with two side walls of the freezing tube placing frame 2.
Furthermore, an anti-skid buffer gasket is arranged on the contact surface of the locking device and the freezing storage pipe.
Further, locking device is the clamp, and the one end of clamp is articulated with a lateral wall of cryopreserving pipe rack 2, and the other end of clamp and another lateral wall bolted connection of cryopreserving pipe rack 2.
The inserted sheet only can be to freezing the fixed effect at both ends about depositing the pipe realization, in order to make freezing the pipe and also reach fixed effect in the upper and lower direction, consequently set up locking device on two lateral walls of freezing the pipe rack 2, this locking device span on freezing the pipe and be parallel to each other with draw-in groove 3. This locking device should set up to the bellied arc that makes progress, and this radian should with the circularity phase-match of the cryopreserved pipe of placing, and then make locking device can with cryopreserved pipe perfect laminating when locking state, reach better fixed effect. In order to facilitate the taking and placing of the freezing storage tubes, the locking device and the freezing storage tube placing frame 2 are detachably connected. Locking device chooses for use in this embodiment for radian and cryopreserved pipe radian assorted clamp, also can select other locking device in practical application, as long as can satisfy the cryopreserved pipe simultaneously and can dismantle two conditions with cryopreserved pipe rack 2.
Because the freezing pipe is mostly made of glass and plastic materials, the outer surface of the freezing pipe is smooth, and therefore, in order to avoid the relative sliding of the freezing pipe with respect to the locking device, the anti-skidding buffer gasket is arranged on the contact surface of the locking device and the freezing pipe. The anti-slip buffer pad can further overcome the brittleness of glass or plastic materials, and the frozen tube cannot be broken in the locking process. In practical application, a softer material with a larger friction coefficient can be selected to manufacture the anti-skid buffer pad, such as silica gel or rubber.
Further, a handle 5 is arranged at the top of the outer side of the upper cover 4.
Furthermore, a sealing gasket is arranged on the contact surface of the upper cover 4 and the top opening of the freezing and thawing box 1.
In order to facilitate the gripping of the upper cover 4, a handle 5 is provided on the top of the upper cover 4. The arrangement of the sealing washer can ensure that the freezing and thawing box 1 has better heat preservation and cold insulation effects, and can better isolate the pollution of the external environment, so that the cell freezing and thawing process is safer and more sanitary.
Further, the side wall of the freezing and thawing box 1 positioned below the freezing and depositing tube placing rack 2 is provided with a through hole and is communicated with a liquid nitrogen tank through a guide tube. In general, the freezing process in the cell freezing and thawing process is realized by liquid nitrogen in a low-temperature environment, so that through holes are formed in the side wall of the freezing and thawing box 1 to enable the freezing process to be communicated with an external liquid nitrogen tank through a conduit. Liquid nitrogen can be delivered into the freezing and thawing box 1 by a mechanical pump during the freezing and thawing process. The through holes are formed below the freezing and depositing tube placing frame 2, so that liquid nitrogen can be slowly filled from the bottom of the freezing and thawing box 1, and the phenomenon that the liquid nitrogen overflows from the upper part in the filling process is avoided.
The specific application method of the fixing device for the cell freezing and thawing process in this embodiment is as follows: firstly, sterilizing and disinfecting the cryopreserved pipes, the cryopreserved pipe placing frame 2, the locking device, the inserting pieces and the freezing and thawing box 1; then placing the cells to be subjected to cell freeze thawing and the culture medium or the extraction solution required by the cells into a freezing tube and sealing the mouth of the freezing tube; after sealing is finished, the cryopreservation pipe is placed in the corresponding arc-shaped groove 6 in a lying mode, then the inserting pieces with the corresponding sizes are inserted into the inserting grooves 3 at the left end and the right end of the cryopreservation pipe, the clamp hoop is sleeved in the middle of the cryopreservation pipe after the inserting pieces are stably installed, and the tightness degree of bolt connection between the movable end of the clamp hoop and the other side wall of the cryopreservation pipe placing rack 2 is adjusted according to the diameter of the cryopreservation pipe until the cryopreservation pipe is firmly fixed; after all the freezing tubes are fixed in the arc-shaped grooves 6, placing all the freezing tube placing frames 2 inside the freezing and thawing box 1, and connecting the freezing tube placing frames 2 with the freezing and thawing box 1 through the connecting parts 7 and the bolts on the side walls of the freezing and thawing box 1; after all the freezing and depositing tube placing racks 2 are fixed, the upper cover 4 is covered on the opening of the freezing and thawing box 1 to seal the freezing and depositing tube placing racks; starting a mechanical pump to charge liquid nitrogen from the liquid nitrogen tank into the freeze-thaw box 1, and closing the mechanical pump to stop inputting the liquid nitrogen after the liquid nitrogen amount meets the requirement; then, carrying out a freezing process in the cell freezing and thawing process; after the freezing process is finished, an electric heating plate control device is started to heat the electric heating plate and control the temperature of the electric heating plate, and after the set temperature is reached, a thawing and reviving process in the cell thawing process is carried out; repeating the freezing and thawing processes for multiple times until the cells are cracked or in the required cell state, stopping freezing and thawing the cells, and opening the upper cover 4 to take out the freezing and preserving tube.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A fixation device for use in a cell freezing and thawing process, comprising:
the freezing and thawing box comprises a freezing and thawing box (1), a freezing and thawing tube placing frame (2) and a freezing and thawing box (1), wherein the freezing and thawing box is detachably connected with the side wall of the freezing and thawing box (1) and is arranged inside the freezing and thawing box (1), and an arc-shaped groove (6) matched with the radian of a freezing and thawing tube is formed in the top of the freezing and thawing tube placing frame (2);
the clamping grooves (3) are arranged in the arc-shaped grooves (6), a plurality of clamping grooves (3) are arranged, each clamping groove (3) is provided with an inserting piece with a corresponding size in a matched mode, and the interval between every two adjacent clamping grooves (3) in each arc-shaped groove (6) is matched with the height of the cryopreservation pipe placed in the arc-shaped groove (6);
and the upper cover (4) is covered on the top opening of the freezing and thawing box (1).
2. A fixation device for a cell freeze-thaw process according to claim 1, wherein a heating device is provided outside the cryopreservation tube rack (2).
3. The fixing device for a cell freezing and thawing process according to claim 1, wherein the freezing and thawing tube placing rack (2) is detachably connected with the side wall of the freezing and thawing box (1) through connecting parts (7) arranged at two ends.
4. The fixing device for a cell freezing and thawing process according to claim 1, wherein the freezing tube placing rack (2) between two adjacent clamping grooves (3) is provided with a locking device parallel to the clamping grooves (3), and the locking device is detachably connected with two side walls of the freezing tube placing rack (2).
5. A fixation device for a cell freeze-thaw process according to claim 1, wherein a handle (5) is provided at the top of the outer side of the upper cover (4).
6. A fixation device for a cell freeze-thaw process according to claim 1, wherein a sealing gasket is arranged on a contact surface of the upper cover (4) and a top opening of the freeze-thaw box (1).
7. A fixing device for a cell freezing and thawing process according to claim 1, wherein the side wall of the freezing and thawing box (1) below the freezing and thawing tube placing rack (2) is provided with through holes and is communicated with a liquid nitrogen tank through a conduit.
8. A fixation device for use in a cell freeze-thaw process according to claim 2, wherein the heating device is a water bath heating device or an electric hot plate.
9. The fixing device for a cell freezing and thawing process as claimed in claim 4, wherein the locking device is a clamp, one end of the clamp is hinged with one side wall of the freezing tube placing rack (2), and the other end of the clamp is bolted with the other side wall of the freezing tube placing rack (2).
10. The fixing device for cell freezing and thawing process according to claim 4 or 9, wherein the contact surface of the locking device and the freezing tube is provided with an anti-slip buffer gasket.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922402858.1U CN211771226U (en) | 2019-12-27 | 2019-12-27 | A fixing device for cell freeze thawing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922402858.1U CN211771226U (en) | 2019-12-27 | 2019-12-27 | A fixing device for cell freeze thawing process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211771226U true CN211771226U (en) | 2020-10-27 |
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ID=72979771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922402858.1U Active CN211771226U (en) | 2019-12-27 | 2019-12-27 | A fixing device for cell freeze thawing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211771226U (en) |
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2019
- 2019-12-27 CN CN201922402858.1U patent/CN211771226U/en active Active
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