CN211713094U - PCR temperature detection test tube - Google Patents
PCR temperature detection test tube Download PDFInfo
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- CN211713094U CN211713094U CN201922461906.4U CN201922461906U CN211713094U CN 211713094 U CN211713094 U CN 211713094U CN 201922461906 U CN201922461906 U CN 201922461906U CN 211713094 U CN211713094 U CN 211713094U
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- Prior art keywords
- pcr
- temperature
- test tube
- pipe
- temperature sensor
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- 238000001514 detection method Methods 0.000 title claims abstract description 26
- 238000012360 testing method Methods 0.000 title claims abstract description 23
- 238000009529 body temperature measurement Methods 0.000 claims abstract description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 description 17
- 238000000034 method Methods 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
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Abstract
The utility model discloses a PCR temperature detection test tube, PCR temperature detection test tube includes PCR pipe, temperature sensor follows the center pin direction fixed connection of PCR pipe is in the socle center department of PCR pipe, temperature sensor's data line runs through the well upper portion pipe wall connection of PCR pipe is to temperature measurement equipment. The utility model provides a PCR temperature detection test tube, simple structure, the good reliability, stability is good, and the real-time is good to can compatible various PCR appearance commonly used.
Description
Technical Field
The invention relates to the field of temperature performance detection of a PCR instrument, in particular to a test tube for detecting the temperature performance of each hole site of the PCR instrument.
Background
In the PCR, DNA is denatured at high temperature (usually about 95 ℃) to become single-stranded in vitro, and at low temperature (usually about 60 ℃) a primer and a single-stranded DNA are bound by the principle of base complementary pairing, and then the temperature is adjusted to the optimum reaction temperature (usually about 72 ℃) for DNA polymerase, and the DNA polymerase synthesizes a complementary strand in the direction from phosphate to deoxyribose (5 '-3'). The PCR instrument manufactured based on DNA polymerase is actually a temperature control device, and can accurately and timely carry out conversion control among denaturation temperature, renaturation temperature and extension temperature. In order to provide a good PCR environment, a temperature control unit must be included in the PCR instrument, and the unit must also have controlled temperature performance: temperature accuracy, temperature uniformity, rapid temperature rise and fall, and the like. The conventional PCR instruments have 8, 12, 24, 48, 96, 384 and the like fluxes, and the consistency and repeatability of the PCR process can be ensured only by accurately controlling the temperature of the multi-flux PCR instrument.
In order to accurately control the temperature of the flux corresponding positions, real-time and accurate whole-process detection needs to be carried out on the positions, and an effective tool for detecting the temperature control system of the PCR instrument is needed either for evaluating the temperature control performance of the PCR instrument or for after-sales maintenance of the temperature control system of the PCR instrument. A patent with application publication number CN109401956A of 3, 1 and 2019 discloses a test tube for a temperature detector of a PCR instrument, wherein a fluid medium is used for submerging and fixing a temperature sensor, and the medium is used for simulating an actual PCR reaction system; the pin of the temperature sensor is led out from the pipe orifice. The PCR reaction is actually a closed system liquid phase chemical reaction, the design of patent CN109401956A is actually an open system simulating a PCR reaction system, the difference between the actual PCR reaction process and the actual PCR reaction process is large, and although the temperature control system of the PCR instrument can be detected to a certain extent, the detection is not accurate enough.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a make things convenient for accurate detection PCR appearance temperature performance's PCR temperature detection test tube, it can simulate the sample reaction completely, simple structure, and the good reliability, stability is good, and the real-time is good to can compatible various PCR appearance commonly used.
The utility model provides a PCR temperature detection test tube, including PCR pipe, temperature sensor follows the center pin direction fixed connection of PCR pipe is in the socle center department of PCR pipe, temperature sensor's data line runs through the well upper portion pipe wall of PCR pipe is connected to temperature measurement equipment.
In a preferred embodiment of the PCR temperature detection test tube provided in the present invention, the temperature sensor is a thermistor or a thermocouple.
In a preferred embodiment of the PCR temperature detection test tube provided by the present invention, the data line of the temperature sensor and the middle upper tube wall junction of the PCR tube are sealed by silica gel.
Compared with the prior art, the utility model provides a pair of PCR temperature detection test tube has following beneficial effect:
the temperature sensor is fixedly connected to the center of the bottom of the PCR tube along the central axis direction of the PCR tube, the position of the temperature sensor is fixed, the temperature sensor is not influenced by the temperature difference between the tube wall and the center of the PCR tube, the temperature performance of the PCR instrument can be detected by adopting an actual sample to carry out PCR reaction, and the detection result is stable and reliable;
a data line of the temperature sensor penetrates through the middle upper pipe wall of the PCR pipe to be connected to temperature measuring equipment, the temperature sensor can be completely closed to form a closed system for carrying out PCR reaction, the PCR reaction scene is real, transmission data can be collected in real time, the detection result is stable and reliable, and the real-time performance is good;
thirdly, the utility model relates to a simple structure, as long as the PCR pipe of chooseing for use is compatible with PCR structure hole pipe, just can the PCR appearance of the corresponding model of adaptation, and the suitability is good.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive work, wherein:
FIG. 1 is a schematic structural diagram of a PCR temperature detection test tube provided by the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The following describes in detail embodiments of the present invention with reference to the accompanying drawings.
Referring to fig. 1, the present embodiment provides a temperature detector, which has a simple structure, good reliability, good stability, good real-time performance, and is compatible with various commonly used PCR instruments.
The PCR temperature detection test tube provided by the embodiment comprises a PCR tube 1 and a temperature sensor 2, wherein the temperature sensor 2 is fixedly connected to the center of the bottom of the PCR tube 1 along the central axis direction of the PCR tube 1, and a data line of the temperature sensor 2 runs through the middle upper tube wall of the PCR tube 1 and is connected to a temperature measurement device.
In the PCR temperature detection test tube provided in this embodiment, the temperature sensor 2 is not limited to a thermistor or a thermocouple, and is used for testing the temperature in the test tube. The optimal choice provided by the embodiment is a German Heraeus PT 10001/3B-level high-precision +/-0.1 ℃ thin film platinum resistor, and the platinum resistor not only has sensitive temperature response, but also has high repetition precision and good linearity.
In the PCR temperature detection test tube provided by this embodiment, the joint between the data line of the temperature sensor 2 and the middle-upper tube wall of the PCR tube 1 is sealed with silica gel, so as to ensure that the PCR tube 1 is a closed system after being closed.
The utility model provides a PCR temperature detection test tube, PCR pipe 1 be not limited to a certain concrete PCR pipe, as long as it is compatible with the PCR structure hole pipe of corresponding PCR appearance, all the utility model discloses an entitlement scope.
When the temperature of the PCR instrument is detected, firstly, the configured PCR reaction system is loaded into the corresponding PCR tube 1, then the loaded PCR tube 1 is loaded into the PCR instrument, the PCR program is started for reaction, the temperature sensor 2 in the PCR tube 1 collects the temperature data in the tube in real time and transmits the temperature data to the temperature detection equipment, and the real-time and accurate whole-process recording and monitoring of the temperature of the positions corresponding to the fluxes are realized.
Compared with the prior art, the utility model provides a pair of PCR temperature detection test tube has following beneficial effect:
the temperature sensor 2 is fixedly connected to the center of the bottom of the PCR tube 1 along the central axis direction of the PCR tube 1, the position of the temperature sensor 2 is fixed, the temperature sensor is not influenced by the temperature difference between the tube wall and the tube center, the temperature performance of the PCR instrument can be detected by adopting an actual sample to perform PCR reaction, and the detection result is stable and reliable;
a data wire of the temperature sensor 2 penetrates through the middle upper pipe wall of the PCR pipe 1 to be connected to temperature measuring equipment, the closed system can be completely closed to form a closed system for carrying out PCR reaction, the PCR reaction scene is real, data can be collected and transmitted in real time, the detection result is stable and reliable, and the real-time performance is good;
thirdly, the utility model relates to a simple structure, as long as the PCR pipe 1 of chooseing for use is compatible with PCR structure hole pipe, just can the PCR appearance of the corresponding model of adaptation, and the suitability is good.
The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.
Claims (3)
1. The utility model provides a PCR temperature detection test tube, its characterized in that includes the PCR pipe, temperature sensor follows the center pin direction fixed connection of PCR pipe is in the center department of the socle of PCR pipe, temperature sensor's data line runs through the well upper portion pipe wall of PCR pipe is connected to temperature measurement equipment.
2. The PCR temperature detecting test tube according to claim 1, wherein the temperature sensor is a thermistor or a thermocouple.
3. The PCR temperature detecting and testing tube as claimed in claim 1, wherein the junction of the data line of the temperature sensor and the middle and upper tube walls of the PCR tube is sealed with silica gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922461906.4U CN211713094U (en) | 2019-12-31 | 2019-12-31 | PCR temperature detection test tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922461906.4U CN211713094U (en) | 2019-12-31 | 2019-12-31 | PCR temperature detection test tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211713094U true CN211713094U (en) | 2020-10-20 |
Family
ID=72821443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922461906.4U Expired - Fee Related CN211713094U (en) | 2019-12-31 | 2019-12-31 | PCR temperature detection test tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211713094U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2614905A (en) * | 2022-01-24 | 2023-07-26 | Stratec Se | Temperature measuring container, system and method comprising the container |
-
2019
- 2019-12-31 CN CN201922461906.4U patent/CN211713094U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2614905A (en) * | 2022-01-24 | 2023-07-26 | Stratec Se | Temperature measuring container, system and method comprising the container |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201020 |